Translational Oncology
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2025
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IF 4.1
Zhe Qin,Fangzhou Ye,Jiayi Wang,Jun Jiang,Xiaohong Zhang,Huanqing Li,Li Feng
Background Cisplatin resistance significantly impedes the treatment of gastric cancer (GC). This work examined the possible therapeutic target status and function of BUB1B in controlling cisplatin resistance. Methods Following the identification of differentially expressed genes (DEGs), protein-protein interaction (PPI) network analysis was conducted using datasets from the Cancer Genome Atlas-stomach adenocarcinoma (TCGA-STAD), GSE51575, and GSE79973. Functional tests assessed the effect of BUB1B overexpression and knockdown on the GC cells. Enrichment analysis and RNA-seq identified pathways linked to BUB1B . Additionally, the function of BUB1B in GC cells resistant to cisplatin in regulating DNA repair was examined, as its relationship with Rad51 inhibitor (B02) in regulating cell cycle, proliferation, and apoptosis. The combined effects of Rad51 suppression and BUB1B overexpression on tumor development in cisplatin-resistant GC cells were further validated in vivo xenograft models. Results Significant overexpression of six critical overlapping genes was seen in GC tissues. The GC cell invasion, migration, and proliferation processes were improved by BUB1B overexpression, whereas BUB1B knockdown prevented these outcomes. Genes involved in DNA repair were downregulated by BUB1B knockdown, according to an RNA-seq study. BUB1B overexpression boosted cell survival via modulating cell cycle proteins, but BUB1B knockdown hampered DNA repair and increased death in cisplatin-resistant GC cells. Overexpression of BUB1B enhanced tumor development in vivo and counteracted the inhibitory effects of B02 on cell growth. Conclusion BUB1B enhances cisplatin resistance in gastric cancer by regulating DNA repair and cell cycle progression, suggesting that targeting BUB1B may be a feasible therapeutic strategy.
研究领域:
肿瘤学
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分子生物学
·
癌症治疗
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DOI
PubMed
高影响因子
Coatings
·
2022
·Vol. 12
·期 4
·447
·
IF 3.236
Yingxiang Ma,Dan Jiang,Yuping Yang,Li Ma,Jian Zhou,Guosheng Huang
In this study, the urea-formaldehyde (UF)-tung oil solution of phenolic amide (PA) microcapsules to realize anti-fouling and anti-corrosion integration was synthesized by the in situ polymerization method. The compounds and structures were optimized by investigating six kinds of different emulsifiers. The results showed that high-core-content and narrow-particle-size-distribution microcapsules could be synthesized with sodium dodecyl benzene sulfonate (SDBS)/polyvinyl alcohol (PVA), and the core content of the microcapsules was 75 wt% at microcapsule sizes from 24.07 to 71.33 µm. The results of self-healing coatings showed that when the content of microcapsules in the coating exceeded 10 wt%, the healing agent released from the scratched surface could cover the naked metal effectively, which could pass a 7 day neutral salt spray test without rust at the scratched area. A sufficient dose anti-fouling agent can be provided to prevent diatoms and mussels from adhering. The present work shows that the complex emulsifier can better control the particle size distribution and microstructure of the microcapsules, and the admixture of the microcapsules into the resin epoxy coating can realize excellent anti-corrosion and anti-fouling functions.Keywords:self-healing;microcapsules;anti-corrosion;anti-fouling
研究领域:
材料科学
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化学工程
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DOI
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Mingzhen Zhong,Tong Liao,Zehan Zeng,Jiangang Mei,Bodeng Wu,Shan Lin,Yitao Zhao,Yong Tan,Ningcen Li,Qi Xiu,Chunchen Liu,Xiuhua Wu,Chengtao Nie,Huixian Lin,Ye Zhang,Wenbin Li,Bo Li,Weilun Pan,Lei Zheng
Skin barrier impairment is critical in the development of atopic dermatitis (AD), increasing vulnerability to external pathogens and disrupting cell metabolism, which leads to inflammatory stress and immune imbalance. In this study, a natural turmeric-derived nanovesicle (TDNV)-laden metal polyphenol hydrogel, termed Fe-HD@TDNV is proposed, to synergistically restore the compromised skin barrier in AD through a dual-repair strategy. The TDNV effectively regulates metabolic activity by upregulating the expression of skin barrier proteins, antioxidant enzymes, and antimicrobial peptides (AMPs) in keratinocytes, thereby reinforcing barrier integrity and combating pathogens. Simultaneously, the Fe-HD hydrogel, cross-linked by Fe 3 ⁺ ions and hyaluronic acid-graft-dopamine (HD), provides superior skin compatibility and establishes a low oxidative stress environment for potentiating the therapeutic efficacy of TDNV. By improving skin barrier conditions, the Fe-HD@TDNV hydrogel exhibited desirable performance in maintaining better skin hydration, reducing epidermal thickness, and decreasing abnormal immune responses in acute skin disruption models and AD models. This work is expected to offer insights into the cross-kingdom regulation between plant-derived nanovesicles and mammals, as well as the design of disease-specific dual-functional repair strategies.
研究领域:
皮肤病学
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材料科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Guoqiang Zhao,Jing Zhou,Yanning Tian,Qifeng Chen,Dongmei Mao,Jianchun Zhu,Xing Huang
Fomesafen is a diphenyl ether herbicide used to control the growth of broadleaf weeds in bean fields. The persistence, phytotoxicity, and negative impact on crop rotation associated with this herbicide have led to an increasing concern about the buildup of fomesafen residues in agricultural soils . The exigent matter of treatment and remediation of soils contaminated with fomesafen has surfaced. Nevertheless, the degradation pathway of fomesafen in soil remains nebulous. In this study, Bacillus sp. Za was utilized to degrade fomesafen residues in black and yellow brown soils. Fomesafen's degradation rate by strain Za in black soil reached 74.4%, and in yellow brown soil was 69.2% within 30 days. Twelve intermediate metabolites of fomesafen were identified in different soils, with nine metabolites present in black soil and eight found in yellow brown soil. Subsequently, the degradation pathway of fomesafen within these two soils was inferred. The dynamic change process of soil bacterial community structure in the degradation of fomesafen by strain Za was analyzed. The results showed that strain Za potentially facilitate the restoration of bacterial community diversity and richness in soil samples treated with fomesafen, and there were significant differences in species composition at phylum and genus levels between these two soils. However, both soils shared a dominant phylum and genus, Actinobacteriota, Proteoobacteria, Firmicutes and Chloroflexi dominated in two soils, with a high relative abundance of Sphingomonas and Bacillus . Moreover, an intermediate metabolite acetaminophen degrading bacterium, designated as Pseudomonas sp. YXA-1, was isolated from yellow brown soil. When strain YXA-1 was employed in tandem with strain Za to remediate fomesafen contaminated soil, the degradation rate of fomesafen markedly increased. Overall, this study furnishes crucial insights into the degradation pathway of fomesafen in soil, and presents bacterial strain resources potentially beneficial for soil remediation in circumstances of fomesafen contamination.
研究领域:
环境科学
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微生物学
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土壤学
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农学
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产品关联
DOI
PubMed
高影响因子
WASTE MANAGEMENT & RESEARCH
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2024
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IF 3.7
Yan Zhou,Wenhui Liu,Dandan Chen,Bangwei Liu,Ping Lu,Yiwei Zhang
A novel Fe/Ca-modified biochar (BC) derived from corn stalk was prepared in this study for the simultaneous adsorption and immobilization of arsenic, cadmium and lead in aqueous solution and soil, respectively. The adsorption experiment in aqueous solution indicated that 1Ca-Fe@BC exhibited excellent removal efficiency (R, %) towards arsenic (46.3%), cadmium (76.3%) and lead (93.6%). The presence of cadmium and lead could enhance the adsorption efficiency of arsenic by facilitating the formation of ternary complexes with it. Moreover, both cooperative and competitive effects occurred between cadmium and lead adsorption. When the distribution coefficient of cadmium and lead was equimolar, cooperative adsorption prevailed. However, when the distribution coefficient deviated from this balance, competitive adsorption dominated with a higher affinity for lead. The soil culture experiment demonstrated that the utilization of 1Ca-Fe@BC could enhance the stability of arsenic, cadmium and lead in soil by enhancing soil cation exchange capacity and pH while reducing dissolved organic carbon. The competitive and cooperative effects of arsenic, cadmium and lead in soil were consistent with those found in aqueous solution. The results showed that 1Ca-Fe@BC exhibited an excellent remediation efficiency for both arsenic, cadmium and lead-contaminated aqueous solution and soil.
研究领域:
环境科学
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土壤学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 14
·11248
·
IF 5.6
Yang Liu,Chuanlei Zhang,Yanhui Kong,Huiyu Liu,Cheng Chen,Wenyu Gao,Xiaowei Xi,Hui Yang,Linhong Deng
Blindness is frequently caused by corneal abnormalities, and corneal transplantation is the most effective treatment method. It is extremely important to develop high-quality artificial corneas because there are not enough donor corneas accessible for cornea transplantation. One of the most-often utilized materials is collagen, which is the primary component of natural cornea. Collagen-based corneal repair materials have good physicochemical properties and excellent biocompatibility, but how to promote the regeneration of the corneal nerve after keratoplasty is still a big challenge. In this research, in order to promote the growth of nerve cells on a collagen (Col) substrate, a novel collagen-based material was synthesized starting from the functionalization of collagen with unsaturated methacryloyl groups that three-dimensionally photopolymerize to a 3D network of chemically crosslinked collagen (ColMA), onto which taurine molecules were eventually grafted (ColMA-Tr). The physicochemical properties and biocompatibility of the Col, ColMA and ColMA-Tr films were evaluated. By analyzing the results, we found that all the three samples had good moisture retention and aq high covalent attachment of methacryloyl groups followed by their photopolymerization improved the mechanical properties of the ColMA and ColMA-Tr. Most importantly, compared with ColMA, the taurine-modified collagen-MA film significantly promoted the growth of nerve cells and corneal epithelial cells on its surface. Our preliminary results suggest that this novel ColMA-Tr film may have potential use in cornea tissue engineering in the future.Keywords:collagen;corneal repair;nerve regeneration
研究领域:
生物材料学
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眼科医学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Jianli Jia,Ben Zhang,Aoran Li,Weiran Wang,Bing Xiao,Xiaolong Gao,Haokun Yuan,Yuxin Han,Xiwang Zhao,Ravi Naidu
Microbial technologies hold great promise for in situ remediation of polycyclic aromatic hydrocarbons (PAHs)-contaminated soils. However, the selection of enhancement measures and corresponding remediation strategies remains insufficiently understood. In this study, a series of enhancement treatments, including bacterial consortium inoculation (comprising Achromobacter denitrificans BP1, Rhodococcus aetherivorans BW2, and Lysinibacillus sp. BS3), nutrient addition, and bio-ventilation, were implemented to develop effective in situ remediation strategies for PAHs-contaminated soil. Over a 60-day incubation, the enhancement treatments achieved phenanthrene (PHE) degradation efficiencies of 68.0–94.7 % and benzo[a]pyrene (BaP) degradation efficiencies of 12.9–82.4 %. Degradation rates across soil layers followed the pattern: upper layer > lower layer > middle layer. Enhancement treatments significantly boosted soil dehydrogenase (DH) and fluorescein diacetate (FDAH) activities. Among these, the sequential consortium inoculation with nutrient addition treatment (T6) demonstrated the highest degradation efficacy. In the treatment T6, the relative abundance of consortium genera was significantly increased, playing critical roles in PAHs degradation. The connectivity and stability of the soil bacterial network were enhanced, providing greater resilience to pollutants. Quantitative PCR analysis showed that the enhancement strategy increased RHDα-GN gene abundance by 1.98-fold at the initial and maintained a positive correlation with PAHs residues throughout the process (p < 0.05), and the phe gene exhibited a continuous upward trend during remediation, ultimately reaching 1.61–1.96 times its initial abundance. Overall, this study provides a strong candidate of integrated enhancement strategies to advance in situ bioremediation of PAH-contaminated sites.
研究领域:
环境科学
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微生物学
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生物技术
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土壤科学
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生态毒理学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Pengcheng Xiao,Hui Yuan,Yiting Lei,luhan Bao,Fei Wang,Juan Wang,Wenguo Cui,Wei Huang
The lubrication barrier, constituted by hydrated structures, serves as an essential protective mechanism in dynamic tissues. Its absence renders interfacial tissues susceptible to mechanical stress and inflammation, thereby impeding the repair processes, as seen in osteoarthritis. This study presents a microsphere-based lubrication barrier that forms a stable, hydrated layer, effectively blocking inflammatory communication between the surrounding matrix and deep cartilage layers. Hydrogel microspheres (CL@Lipo-w@AHAMs) are developed using gas-shear microfluidics and photopolymerization, incorporating a collagen-targeting liposome, aldehyde-functionalized hyaluronic acid methacrylate, and methacrylated alginate. These liposomes stick to cartilage, creating a hydration barrier that prevents inflammation communication. Ex vivo tests on bovine cartilage reveal that microspheres reduce friction to 0.036 ± 0.002 and decrease inflammation-related friction damage by 70.64%. In vivo, these microspheres improve cartilage repair and reduce inflammation. Overall, this lubrication barrier system prevents inflammatory communication between injured tissues, presenting a promising strategy for dynamic tissue repair.
研究领域:
生物医学工程
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组织工程
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材料科学
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炎症生物学
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骨科
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Zhiguo Wu,Dan Liu,Ying Deng,Runyi Pang,Jinjin Wang,Tian Qin,Zongzheng Yang,Rongliang Qiu
In this research, a new material, chitosan/polypyrrole (CS/PPy), was synthesized and linked with the Cr(VI)-reducing bacterial strain YL3 to treat Cr(VI)-polluted soil. The findings demonstrated that the synergistic application of strain YL3 and CS/PPy achieved the greatest reduction (99.6 %). During the remediation process, CS/PPy served as a mass-storage and sustained release agent in the soil, which initially decreased the toxic effects of high concentrations of Cr(VI) on strain YL3, thereby enhancing the Cr(VI) reduction efficiency of strain YL3. These combined effects significantly mitigated Cr(VI) stress in the soil and restored enzyme activities . Furthermore, wheat growth in the treated soil also significantly improved. High-throughput sequencing of the microorganisms in the treated soil revealed that CS/PPy was not only effective at removing Cr(VI) but also at preserving the original microbial diversity of the soil. This suggests that the combined treatment using strain YL3 and CS/PPy could rehabilitate Cr(VI)-contaminated soil, positioning CS/PPy as a promising composite material for future bioremediation efforts in Cr(VI)-contaminated soils.
研究领域:
环境科学
·
生物技术
·
土壤学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Kaiming Zhang,Jiawen Sun,Jingyao Song,Chuanhui Gao,Zhe Wang,Chengxin Song,Yumin Wu,Yuetao Liu
Flexible conductive composites can be used as wearable strain sensors, which are widely used in the fields of new-generation robotics, electronic skin, and human detection. However, how to make conductive composites that simultaneously possess flexibility, stretchability, self-healing, and sensing capability is challenging research. In this work, we innovatively designed and prepared a silicone polymer conductive composite. MXenes and amino poly(dimethylsiloxane) were modified by small biomolecules via an esterification reaction and a Schiff base reaction, respectively. The modified MXenes are uniformly dispersed, which endows the composite with good electrical conductivity. The reversibility of multiple hydrogen bonds and imine bonds in the composite system makes it have ideal tensile properties and high-efficiency self-healing ability without external stimulation. The conductive composite containing 10 wt % A-MXenes showed an elongation of 81%, and its mechanical strength could reach 1.81 MPa. After repair, the tensile properties and the electrical conductivity could be restored to 98.4 and 97.6%, respectively. In addition, the conductive composite is further evaluated for the value of wearable strain sensors. Even after cut-healed processes, the conductive composite can still accurately detect tiny human movements (including speaking, swallowing, and pressing). This kind of self-healing MXene/PDMS elastomers based on the modification of small biomolecules has great potential as wearable strain sensors. This simple preparation method provides guidance for future multifunctional flexible electronic materials.
研究领域:
材料科学
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纳米技术
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可穿戴技术
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电子皮肤
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机器人技术
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 41
·62272-62280
·
IF 5.19
Wang Xiaowen,Wang Yanjie,Zhao Xiaoyan,Chen Bingqiong,Kong Nannan,Shangguan Lingfei,Zhang Xiaobin,Xu Yingchun,Hu Feng
It has been well documented that polycyclic aromatic hydrocarbon (PAHs) can be taken up from the environment by the plants and translocated into the shoots. However, the mechanisms underlying this process are poorly understood. Nelumbo nucifera L. (lotus) is a highly ornamental aquatic plant known to possess strong phytoremediation capability. In the present study, the association between phenanthrene (Phe) and nutrients, including nitrogen (N) and phosphorus (P), in lotus was investigated. Over 2 years, all eight lotus cultivars tested accumulated Phe to various degrees when grown in PAH-polluted sediment (0.46 mg/kg Phe). Cluster analysis showed N. nucifera 'Zhongguo Hong Beijing (ZHB)' was the one with the highest Phe levels in the leaves and petals in 2 years. The Phe concentrations in the tissues of 'ZHB' were 3.14 mg/kg and 1.63 mg/kg on average in the first and second year, respectively. Interestingly, 'ZHB' was also the cultivar with the lowest N and P levels considering 2 years and tissues. Hydroponic studies further revealed a negative association between the concentrations of Phe and those of N and P in the aerial tissues under 0.5 and 1.0 mg/L Phe treatments in 'ZHB'. Furthermore, the significant reductions of the roots number (72.6%), longest root length (75.8%), and petiolar height (34.6%) in 'ZHB' seedlings exposed to 1.0 mg/L Phe were observed, indicating that Phe retarded the growth of lotus. These results provide a new understanding of the accumulation of Phe in plants and the association with nutrients and enrich the basis of phytoremediation to the contaminated environment.
研究领域:
环境科学
·
植物学
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DOI
高影响因子
Materials
·
2022
·Vol. 15
·期 23
·8671
·
IF 3.748
Yuan Li,Xirong Lin,Zhanpeng Li,Jinyun Liu
A composite composing of TiO2nanoparticles load on biomass rice husk (RH) is developed by directly growing TiO2nanoparticles on RH. The in-situ growth of the nanocrystals on RH is achieved by a low-cost and one-step homogeneous precipitation. Rapid hydrolysis proceeds at 90 °C by using ammonium fluotitanate and urea to facilitate the selective growth of TiO2. The method provides an easy access to the TiO2-RH composite with a strong interaction between TiO2nanoparticles and the underlying RH. The structure and composition of TiO2-RH are characterized by using X-ray diffraction, X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, and UV-vis absorption spectroscopy. TiO2nanoparticles-RH exhibits a good photocatalytic degradation of methyl orange. The results show that 92% of methyl orange (20 mg L−1) can be degraded within three hours in visible light. The catalytic activity of the composite is not reduced after 6 cycles, and it still reaches 81% after 6 cycles. The enhanced performance is ascribed to the suitable particle size the good dispersibility. It is expected that the high photocatalytical performance and the cost-effective composite presented here will inspire the development of other high-performance photocatalysts.Keywords:TiO2;rice husk;photodegradation;methyl orange;ecological restoration
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）