ANALYTICAL CHEMISTRY
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2014
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IF 5.825
Qiaoling Yu,Xu Wang,Yixiang Duan
A novel capillary-based three-dimensional (3D) fluoroimmunosensor for carcinoembryonic antigen detection was explored for the first time. The immunosensor was designed in symmetrical cylinder structure and fabricated with capillary tubes encapsulated in a quartz tube. The 3D design of the sensor increased the area of sensing surface, flexibility in light path design and efficiency of fluorescence collection by aluminum foil, resulting in analytical performance improvement. The CEA immunosensor was constructed in double antibody sandwich format. Fluorescence signals from DyLight 550-labeled antibody were measured using a laser-induced fluorescence spectrometry. There is an obvious improvement in the linear detection range of 0.7–80 ng/mL. This novel 3D immunosensor dramatically improved the detection limit (1.1 pmol/L CEA) and sensitivity. Assay validation studies indicated that the correlation coefficient reached 0.9935, with recoveries of 92.82–118.81%. Furthermore, the immunosensor was successfully applied to CEA determination in actual saliva specimens with high sensitivity and acceptable precision. Regarding accuracy, the results obtained by 3D immunosensor were not significantly different (t test) from those obtained by validated electrochemiluminescence immunoassay. This new 3D CEA immunosensor was demonstrated to be a high-performance tool for CEA diagnostics.
研究领域:
生物医学工程
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 2
·1-10
·
IF 5.3
Shi Yuechan,Hu Beibei,Liu Baoyuan,Li Xiaomeng,Jing Yongshuai,Li Zhongqiu,Li Zhiwei,Sun Shiguo
An aptamer-antibody sandwich electrochemical immunosensor was studied. Fe 3 O 4 /MWCNTs-COOH/Nafion was modified and fixed on a glassy carbon electrode to amplify electrical signals. The antibody was coupled with AuNPs to form conjugates. It captures the target free thyroxine (FT4) and forms antibody-AuNPs/FT4 macromolecular complexes. The complex is then captured by the aptamer on the glassy carbon electrode and detected by the electrochemical workstation using differential pulse voltammetry (DPV). Under the best conditions, the prepared sensor showed a linear relationship in the concentration range 0.1 to 10 5 pg/mL and the detection limit (LOD) was 0.04 pg/mL. The sensor can enhance the adsorption capacity of target FT4 and detect FT4 stably and with high selectivity. The aptamers and antibodies are free from additional modifications. In addition, the high recovery of the proposed sensor was verified by testing the human serum samples. The immunosensor has the advantages of simple operation, rapid recognition process, and short response time. These results indicate that the immunosensor is expected to achieve rapid and effective detection of FT4 in early pregnancy and evaluate thyroid function in the future. The research ideas of this work will also be applicable to the detection of other small molecules and provide ideas for the detection of other substances. Graphical
研究领域:
生物医学工程
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分析化学
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DOI
PubMed
高影响因子
Analytical Methods
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2025
·
IF 2.6
Yu Wang,Xiaoming Ma
The development and research of electrochemical immunoassay have attracted considerable attention. However, traditional electrochemical immunoassays inevitably require the participation of biological enzymes, which suffer from high cost, poor stability, inconvenient storage and easy inactivation. Herein, we constructed a bio-enzyme-free electrochemical immunoassay system specifically for the detection of aflatoxin B1(AFB1). This method was based on the traditional antigen-antibody immune recognition system while utilizing nanosilver particles (NSP) as signal markers to replace conventional natural enzymes. Subsequently, the labeled NSP was transferred to silver ions in the presence of nitric acid. The concentration of silver ions was detected using anodic stripping voltammetry (ASV), which correlates closely to the concentration of the target. The current intensity measured by the bio-enzyme-free electrochemical sensor exhibited a negative correlation with the concentration of AFB1. Under optimized conditions, the electrochemical sensor was used to detect AFB1 in the dynamic concentration range of 0.01~100 ng/mL, and the limit of detection was 0.4882 pg/mL. The recovery range of AFB1 spiked in corn starch was determined to be between 100.98% and 109.42%, while the relative standard deviation (RSD) range was found to be from 0.34% to 3.82%. These results indicate that the electrochemical immunosensor without biological enzyme labeling has reliable accuracy, and the sensor has a broad application prospect in AFB1 detection.
研究领域:
食品安全
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分析化学
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生物传感
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
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2024
·
IF 2.9
Cheng Ming,Zhang Duo,Cui Jianli,Chen Min,Mi Lei
A novel immunodetection sensing platform for Salmonella based on a CoFe-MOF-carbon nanotube composite was developed. The CoFe-MOF-CNT composite exhibited excellent electrochemical performance, providing a highly conductive and stable matrix for antibody immobilization and signal amplification. Under optimized conditions, the immunosensor demonstrated a wide linear range of 10 2 to 10 7 CFU/mL, with a low detection limit of 37 CFU/mL and a high sensitivity of 6.061 µA/log(CFU/mL). The immunosensor showed excellent specificity towards Salmonella, with a relative response of 100% for Salmonella and less than 10% for non-target bacteria. The immunosensor exhibited good reproducibility (RSD of 4.2%) and stability, retaining 88.7% of its initial response after 30 days of storage at 4 °C. The practical applicability of the immunosensor was demonstrated by analyzing spiked milk samples, with recoveries ranging from 96.7% to 105.2% and RSD less than 6.5%.
研究领域:
生物医学工程
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分析化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-12
·
IF 5.3
Ouyang Ruizhuo,Feng Meina,Liu Jinyao,Wang Caihong,Wang Zhongmin,Hu Xin,Miao Yuqing,Zhou Shuang
Rapid and sensitive detection of carcinoembryonic antigen (CEA) is of great significance for cancer patients. Here, molybdenum (Mo) was doped into bismuth oxide (Bi 2 O 3 ) by one-pot hydrothermal method forming porous tremella Bi 2 MoO 6 nanocomposites with a larger specific surface area than the spherical structure. Then, a new kind of hydrangea-like TiO 2 /Bi 2 MoO 6 porous nanoflowers (NFs) was prepared by doping titanium into Bi 2 MoO 6 , where titanium dioxide (TiO 2 ) grew in situ on the surface of Bi 2 MoO 6 nanoparticles (NPs). The hydrangea-like structure provides larger specific surface area, higher electron transfer ability and biocompatibility as well as more active sites conducive to the attachment of anti-carcinoembryonic antigen (anti-CEA) to TiO 2 /Bi 2 MoO 6 NFs. A novel label-free electrochemical immunosensor was then constructed for the quantitative detection of CEA using TiO 2 /Bi 2 MoO 6 NFs as sensing platform, showing a good linear relationship with CEA in the concentration range 1.0 pg/mL ~ 1.0 mg/mL and a detection limit of 0.125 pg/mL (S/N = 3). The results achieved with the designed immunosensor are comparable with many existing immunosensors used for the detection of CEA in real samples. Graphical abstract
研究领域:
生物医学工程
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分析化学
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材料科学
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DOI
PubMed
高影响因子
Carbon Letters
·
2023
·Vol. 34
·期 3
·907-915
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IF 4.5
Yang Jing,Wang Xingyuan,Zhou Tingting,Wei Liangjun,Guo Meiling,Liu Yanan,Sun Xiaoqi,Wang Yanjie
This paper presents the construction and characterization of an amperometric immunosensor based on the graphene/gold nanoparticles (AuNPs/GO) nanocomposite for the detection of the bladder cancer biomarker, apolipoprotein A1 (Apo-A1). The morphological analysis of the AuNPs/GO nanocomposite demonstrated an almost spherical shape of AuNPs and the successful coverage of their surface by graphene oxide. An increased G peak and decreased D peak after the association of AuNPs with GO, implied a reduction in graphene defects. The X-ray photoelectron spectroscopy (XPS) indicated a significant decrease in the quantity of oxygen-containing functional groups in the AuNPs/GO nanocomposite, as compared to the original GO. Furthermore, the developed sensor demonstrated commendable sensitivity and selectivity, with a wide linear range for Apo-A1 detection. Importantly, the immunosensor exhibited remarkable stability over a period of 14 days, signifying its potential for practical applications.
研究领域:
生物医学工程
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分析化学
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癌症研究
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DOI
高影响因子
TALANTA
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2020
·
IF 5.339
Wenjun Li,Yue Yang,Chaoyun Ma,Yiju Song,Xiuwen Qiao,Chenglin Hong
Traditional sandwich-type electrochemical immunosensors can only detect single tumor markers because signal interference occurs when detecting multiple tumor markers. In this work, an electrical signal difference strategy was proposed for the accurate detection of multiple tumor markers. We labeled PdAgCeO 2 mesoporous nanospheres with a carcinoembryonic antigen (CEA) secondary antibody and MnO 2 nanosheets labeled with an alpha-fetoprotein (AFP) secondary antibody. The two electrical signal tags were mixed and incubated on a prepared immunosensor to catalyze H 2 O 2 and generate an electrical signal I 1 (i-t ampere curve). When 2.5 mM ascorbic acid solution (AA) was added to 20 mL of PBS solution at pH = 6.5 for 180 s, an electrical signal I 2 was generated. I 2 was the current response of the CEA antigen concentration, and the electrical signal difference ΔI = I 1 – I 2 was the current response of the AFP antigen. Thus, the immunosensor accurately detected the AFP and CEA tumor markers. This method was called the electrical signal difference strategy. The proposed single-use immunosensor detected CEA antigens in a range of 0.001 ng/mL-40 ng/mL, and the detection limit was 0.5 pg/mL; the detection range of the AFP antigen was 0.005 ng/mL-100 ng/mL, and the detection limit was 1 pg/mL. Therefore, this study provides new ideas and strategies for accurate clinical detection of multiple tumor markers.
研究领域:
生物医学工程
·
临床诊断
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
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IF 7.145
Guanghui Li,Min Zhu,Lu Ma,Junrong Yan,Xiaoling Lu,Yanfei Shen,Yakun Wan
A phage display library of variable domain of the heavy chain only antibody or nanobody (Nb) was constructed after immunizing a bactrian camel with testosterone. With the smaller molecular size (15 kDa), improved solubility, good stability, high affinity, specificity, and lower immunogenicity, Nbs are a promising tool in the next generation of diagnosis and medical applications. Testosterone is a reproductive hormone, playing an important role in normal cardiac function and being the highly predictive marker for many diseases. Herein, a simple and sensitive immunosensor based on electrochemical impedance spectroscopy (EIS) and Nbs was successfully developed for the determination of testosterone. We successfully isolated the antitestosterone Nbs from an immune phage display library. Moreover, one of the Nbs was biotinylated according to in vivo BirA system, which showed the highest production yield and the most stable case. Further, the EIS immunosensor was set up for testosterone detection by applying the biotinylated antitestosterone Nb. As a result, the biosensor exhibited a linear working range from 0.05 to 5 ng mL–1 with a detection limit of 0.045 ng mL–1. In addition, the proposed immunosensor was successfully applied in determining testosterone in serum samples. In conclusion, the proposed immunosensor revealed high specificity of testosterone detection and showed as a potential approach for sensitive and accurate diagnosis of testosterone.
研究领域:
生物技术
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医学诊断
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分析化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 11
·4269-4277
·
IF 4.58
Zheng Yun,Wang Huiqiang,Ma Zhanfang
The authors describe a method for signal amplification of label-free voltammetric immunosensors. A glassy carbon electrode (GCE) was modified with Prussian Blue-platinum nanoparticles (PB-PtNPs) as a redox-active species that gives a strong amperometric signal at 0.18 V (vs. Ag/AgCl). Benefitting from the excellent electrical conductivity and the strong catalytic activity to H 2 O 2 , the modified GCE gives a strongly enhanced signal. The PB-PtNPs were incorporated into a polyaniline (PANI) hydrogel to further enhance the signal. The signal response of the PB-PtNP-PANI/GCE is larger by a factor of 7.6 than that of PB-PtNP/GCE. In order to further improve electrical conductivity and immobilize antibody, gold nanoparticles (AuNPs) were deposited on the surface of the PB-PtNP-PANI hydrogel. The AuNP-PB-PtNP-PANI hydrogel nanocomposite on the GCE was used in an immunosensor for the model analyte carcinoma antigen 125 (CA125), a biomarker for epithelial ovarian cancer, by immobilizing the respective antibody on the modified GCE. A linear response found for the 0.01 to 5000 U mL −1 CA125 concentration range, with a detection limit of 4.4 mU mL −1 (at an S/N ratio of 3). The electrochemical sensitivity is as high as 119.76 μA·(U/mL) −1 ·cm −2 . The detection of CA125 in human serum showed satisfactory accuracy compared to a commercial chemiluminescent microparticle immunoassay (CMIA).
Schematic of a nanocomposites consisting of gold nanoparticles, Prussian Blue, platinum nanoparticles and polyaniline hydrogel as a signal multi-amplification sensing substrate for the ultrasensitive immuno detection of carcinoma antigen 125 (CA125).
研究领域:
生物医学工程
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电化学传感
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肿瘤标志物检测
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高影响因子
ANALYST
·
2015
·Vol. 140
·期 12
·3989-3995
·
IF 4.107
Yuanjian Liu,Linqun Zhang,Wei Wei,Hongyu Zhao,Zhenxian Zhou,Yuanjian Zhang,Songqin Liu
Early and accurate diagnosis is considered the key issue to prevent the further spread of viruses and facilitate influenza therapy. Herein, we report a colorimetric immunosensor for influenza A virus (IAV) based on gold nanoparticles (AuNPs) modified with monoclonal anti-hemagglutinin antibody (mAb). The immunosensor allows for a fast, simple, and selective detection of IAV. In this assay, influenza-specific antibodies are conjugated to AuNPs to create mAb–AuNP probes. Since IAV has multiple recognition sites for probes on the surface, the mAb–AuNP probes can be specifically arranged on the virus surface due to their very specific antigen recognition. In this case, this aggregation of the mAb–AuNP probes produces a red shift in the absorption spectrum due to plasmon coupling between adjacent AuNPs, and it can be detected with the naked eye as a color change from red to purple and quantified with the absorption spectral measurements. The aggregate formation is also confirmed with transmission electron microscopy (TEM) imaging and dynamic light scattering (DLS). Under the optimal conditions, the present immunoassay can sensitively measure H3N2 IAV (A/Brisbane/10/2007) with a detection limit of 7.8 hemagglutination units (HAU). This proposed immunosensor revealed high specificity, accuracy, and good stability. Notably, it is a single-step detection using AuNP probes and UV-vis spectrophotometer for readout, and no additional amplification, e.g., enzymatic, is needed to read the result. This assay depends on an ordered AuNP structure covering the virus surface and can be applied to any virus pathogen by incorporating the appropriate pathogen-specific antibody.
研究领域:
病毒学
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纳米生物学
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医学诊断
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Dongmiao Qin,Xiaohua Jiang,Guichun Mo,Jinsu Feng,Biyang Deng
A label-free electrochemiluminescence (ECL) immunosensor is developed to detect alpha-fetoprotein (AFP). Ru (bpy) 3 2+ -doped silica nanoparticles (Ru–SiO 2 ) and gold nanoparticle-modified reduced graphene oxide (rGO@Au) are integrated to form an rGO@Au@Ru–SiO 2 composite. The composite is employed as a carrier for immobilizing the target antibody, and a label-free ECL immunosensor is constructed via antigen–antibody interaction. Owing to the numerous Ru (bpy) 3 2+ molecules in the Ru–SiO 2 , a high ECL signal is acquired. With excellent conductivity and a large surface area, the rGO@Au composite not only accelerates electron transfer but also improved the ECL signal and captures antibodies, thus enhancing ECL response and sensitivity. Moreover, boron nitride quantum dots (BNQDs) are used as rGO@Au@Ru–SiO 2 coreactants, achieving high ECL intensity. The proposed ECL system exhibits significant potential in bioanalysis , which is attributed to the excellent biocompatibility and good stability of BNQDs. Under optimal experimental conditions, the sensor has a linear range of 0.0001 ng mL −1 to 100 ng mL −1 and a limit of detection equal to 0.03 pg mL −1 (S/N = 3). The proposed technique is successfully applied in the susceptible detection of AFP in human serum. The recovery ranges from 99% to 100%, and the RSD is less than 2.8%. The constructed ECL immunosensor exhibits acceptable performance during actual application.
研究领域:
生物分析
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医学诊断
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产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Jie Liu,Zixuan Zhang,Jie Dong,Anyi Chen,Jingfu Qiu,Chaorui Li
As a reliable biomarker to evaluate the severity of sepsis, sensitive and accurate detection of procalcitonin (PCT) is essential. In this study, a dual-mode electrochemical immunosensor based on Au/ZIF-8 as substrate and Pt@Cu 2 O as signal label was constructed for the detection of PCT. By loading Au nanoparticles onto rhombic dodecahedral ZIF-8, the substrate (Au/ZIF-8) has large specific surface area and can immobilize antibody (Ab 1 ) by Au–N bonds. Meanwhile, hollow Pt@Cu 2 O nanocomposite with excellent peroxidase-like activity and electrocatalytic activity were synthesized as signal label. In the process of electrochemical testing, Pt@Cu 2 O catalyzed the reduction of hydrogen peroxide (H 2 O 2 ) and further promotes the oxidation of hydroquinone (HQ) to achieve the synergistic amplification of electrochemical signals. The proposed immunosensor detected PCT by amperometric i-t and differential pulse voltammetry (DPV) tests with a good linear response and low limit of detection (i-t: 0.70 fg/mL and DPV: 0.40 fg/mL) in the range of 10 fg/mL∼100 ng/mL. The immunosensor exhibited excellent sensitivity and accuracy, indicating the potential application of this method for PCT detection.
研究领域:
生物医学工程
·
分析化学
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免疫学
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DOI
PubMed
高影响因子
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