POLYMERS FOR ADVANCED TECHNOLOGIES
·
2014
·Vol. 25
·期 6
·613-623
·
IF 1.964
Yiting Xu,Jianjie Xie,Lingnan Chen,Hui Gao,Conghui Yuan,Cong Li,Weiang Luo,Birong Zeng,Lizong Dai
Organic/inorganic hybrid amphiphilic block copolymer poly(methacrylate isobutyl POSS)- b -poly( N -isopropylacrylamide- co -oligo(ethylene glycol) methyl ether methacrylate) (PMAPOSS- b -P(NIPAM- co -OEGMA)) was synthesized via reversible addition–fragmentation chain transfer polymerization. The self-assembly behavior of this block copolymer in aqueous solution was investigated by dynamic light scattering (DLS) and transmission electron microscopy. The results indicate that the novel block copolymer can self-assemble into spherical micelles with PMAPOSS segment as the hydrophobic part and P(NIPAM- co -OEGMA) segment as the hydrophilic part. The temperature-responsive characteristics of the assemblies were tested by UV–Vis spectra and DLS. Some factors such as the concentration, molecular weight, and copolymer generation that may affect the cloud point were studied systematically. The results reveal that this copolymer exhibits a sharp and intensive lower critical solution temperature (LCST). The essentially predetermined LCST can be conveniently achieved by adjusting the content of NIPAM or OEGMA domain. In addition, these novel hybrid micelles can undergo an association/disassociation cycle with the heating and cooling of solution and the degree of reversibility displaying a tremendous concentration dependence, as a novel organic/inorganic hybrid material with distinctive virtues can be potentially used in biological and medical fields, especially in drug nanocarriers for targeted therapy. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
材料科学
·
高分子化学
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纳米技术
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生物医学工程
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DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Suli Hu,Wenhong Hu,Zhenguang Li,Lixia Ren,Yunhui Zhao,Xiaoyan Yuan
Biomedical applications of the synthetic polymers with amino acid side moieties have been studied in light of their biocompatibility. In this work, poly(amino acid-hydroxyethyl methacrylate)s with lysine (Lys)/leucine side moieties were synthesized via reversible addition-fragmentation chain transfer polymerization, and were verified with intrinsic chirality. For biomedical uses, their antibacterial abilities as well as hemolysis and cytotoxicity were investigated. The results indicated that there were similar inhibitions for d - and l -enantiomeric polymers against Staphylococcus aureus when the polymer concentrations were lower than ~ 300 μg/mL, while the polymer containing d -Lys moiety was more effective against Escherichia coli . The d -enantiomers at concentrations < 500 μg/mL showed relatively lower hemolysis, and all the polymers showed good in vitro cell viability with concentrations lower than 200 μg/mL. It was indicated that the antibacterial amino acid-related synthetic polymers could be potentially applied for biomedical applications.
研究领域:
生物材料
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生物医学工程
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药学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 17
·4155-4163
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IF 3.637
Li Jinge,Zhao Liying,Wen Dongxiao,Li Xiaofei,Yang Huaixia,Wang Dazhong,Kong Jinming
In this work, a new method of CYFRA21-1 DNA (tDNA) detection based on electrochemically mediated atom transfer radical polymerization (e-ATRP) and surface-initiated reversible addition-fragmentation chain transfer polymerization (SI-RAFT) cascade polymerization and AgNP deposition is proposed. Firstly, the peptide nucleic acid (PNA) probe is captured on a gold electrode by Au-S bonds for specific recognition of tDNA. After hybridization, PNA/DNA strands provide high-density phosphate groups for the subsequent ATRP initiator by the identified carboxylate-Zr 4+ -phosphate chemistry. Then, a large number of monomers are successfully grafted from the DNA through the e-ATRP reaction. After that, the chain transfer agent of SI-RAFT and methacrylic acid (MAA) are connected by recognized carboxylate-Zr 4+ -carboxylate chemistry. Subsequently, through SI-RAFT, the resulting polymer introduces numerous aldehyde groups, which could deposit many AgNPs on tDNA through silver mirror reaction, causing significant amplification of the electrochemical signal. Under optimal conditions, this designed method exhibits a low detection limit of 0.487 aM. Moreover, the method enables us to detect DNA at the level of PCR-like and shows high selectivity and strong anti-interference ability in the presence of serum. It suggests that this new sensing signal amplification technology exhibits excellent potential of application in the early diagnosis of non-small cell lung cancer (NSCLC).
Electrochemical detection principle for CYFRA21-1 DNA based on e-ATRP and SI-RAFT signal amplification technology.
研究领域:
生物化学
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分析化学
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癌症诊断
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2022
·
IF 4.855
Maolin Wang,Yuting Min,Jinjin Huang,Yi Shi,Xuming Dong,Xiaoli Zhou,Xiaoqin Yu,Dongming Qi,Zan Hua,Tao Chen
Polymer-based catalytic nanoreactors, with the characteristics of easy preparation, good dispersion, and facile modulation of molecular structures, have been widely applied for various organic transformations. Usually, polymeric nanoreactors are fabricated via the self-assembly of amphiphilic copolymers in water, while the disassembly and instability of the relevant nanoreactors often compromise their potential applicability. Molecular brushes (MBs), as a kind of polymer with high-density grafted side chains on the linear polymer main chain, can be rapidly self-assembled into highly ordered nanostructures even at low concentrations. This study reports the fabrication of catalytic nanoreactors from molecular brushes of poly[norbornene–poly(bromoethyl methacrylate-co-methyl methacrylate)]-co-poly[norbornene polyethylene glycol monomethyl ether] (P[NB-(BEMA-co-MMA)]-co-P[NB-PEG]). The amphiphilic molecular brush was synthesized by combining reversible addition–fragmentation chain transfer (RAFT) polymerization and ring-opening metathesis polymerization (ROMP) techniques. Homogeneous catalysts, such as triethylenediamine and 4-(dimethylamino)pyridine analogues, were introduced by nucleophilic substitution with alkyl bromide on the side chain of molecular brushes. Furthermore, micellar catalytic nanoreactors were fabricated via self-assembly in deionized water. The resulted nanoreactors display high catalytic activities toward the Knoevenagel condensation reaction and acylation reaction of alcohol in water, respectively. This contribution describes a general method for constructing highly efficient molecular brush-based catalytic nanoreactors utilizing postpolymerization modification (PPM) for aqueous catalysis.
研究领域:
材料科学
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高分子化学
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催化
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纳米技术
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DOI
高影响因子
Polymer Chemistry
·
2017
·Vol. 9
·期 5
·548-557
·
IF 5.375
Sheng Chen,Xuguang Lv,Xianghui Han,Hang Luo,Chris R. Bowen,Dou Zhang
Dielectric nanocomposites incorporating core–shell structured nanoparticles have drawn increasing attention in recent years for energy storage applications. Among the many key factors, the thickness and the chemical structure of the interfacial layer are rarely investigated. This work presents a novel approach to precisely tailor the interfacial layer thicknesses by modulating the polymerization degree of a rigid liquid-crystalline fluoro-polymer. BaTiO3@rigid-fluoro-polymer nanoparticles with a range of interfacial thicknesses were prepared by a surface-initiated reversible-addition–fragmentation chain transfer polymerization method. The frequency dependent dielectric properties and energy storage capability of dielectric nanocomposites based on a poly(vinylidene fluoride-trifluoroethylene-chlorotrifluoroethylene) P(VDF-TrFE-CTFE) matrix and the modified BaTiO3 nanofiller were investigated. The results demonstrated that the permittivity, breakdown strength and energy density of the polymer nanocomposites were significantly affected by the thickness of the rigid-fluoro-polymer shell around BaTiO3. Moreover, a high discharged energy density of 16.18 J cm−3 was achieved in nanocomposites containing 5 vol% BaTiO3, when the shell thickness was approximately 11 nm. The findings provide a new and innovative approach to prepare dielectric composites with high energy density, and enable a deeper understanding of the influence of the interfacial layer thickness on the dielectric performance.
研究领域:
材料科学
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纳米技术
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能量存储
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聚合物科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2023
·
IF 4.8
Zhiwei Liu,Nan Ma,Shuaibing Yu,Jinming Kong,Xueji Zhang
Ultrasensitive protein detection has considerable potential for early diagnosis and screening of diseases. Here, based on Hemin-catalyzed surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization, this work developed an assay for ultrasensitive fluorescent biosensing of proteins and established a thrombin fluorescent sensor. First, the amino-modified aptamer 1 was immobilized on Fe 3 O 4 magnetic beads, and then thrombin was added to form the aptamer 1-thrombin bioaffinity complex. Another thiol-modified aptamer 2 binds to bound thrombin to form a sandwich structure. Fluorescein O-methacrylate was used as the monomer, acetylacetone (ACAC) as the initiator, and Hemin as the catalyst. Through Hemin-catalyzed SI-RAFT polymerization, a large number of thrombin molecules are labeled with fluorescein O-methacrylate, resulting in severalfold amplification of the fluorescent signal. By detecting the fluorescent signal in thrombin from 1.0 fM to 0.1 nM, the proposed super sandwich strategy can detect thrombin at 0.98 fM, which is lower than the detection limit of most other methods.
研究领域:
分析化学
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生物传感器
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高分子科学
·
蛋白质检测
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DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
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2015
·Vol. 33
·期 3
·456-464
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IF 1.835
Xiao Jun-jie,Li Xiu-bing,Wang Xi,Yi Chun-wang,Su Sheng-pei
The effect of temperature-responsive solution behavior of PNIPAM- b -PPEOMA- b -PNIPAM on its inclusion complexation with α -cyclodextrin was studied. The triblock polymer was prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization and formed inclusion complexes (ICs) after selective threading of the PEO segment of the triblock polymer through the cavities of α -CD units. For comparison, PPEOMA homopolymer was prepared and the inclusion complexation with α -CD was also studied. The ICs were prepared with α -CD when the polymer was in different conformations by changing the temperature, and the formed ICs were characterized by XRD, 1 H-NMR, TGA and DSC. The solutions of the ICs show temperature-responsive clear/turbid transition or fluidic emulsion/gel transition depending on the concentration of the α -CD added, and the stoichiometry determined by 1 H-NMR and TGA indicates that the stoichiometry of EO to α -CD of the resulted ICs increases with increasing of temperature.
研究领域:
高分子科学
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材料化学
·
物理化学
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产品关联
DOI
高影响因子
Polymer Chemistry
·
2016
·Vol. 8
·期 6
·1028-1038
·
IF 5.687
Liyi Fu,Pan Yuan,Zheng Ruan,Le Liu,Tuanwei Li,Lifeng Yan
An amphiphilic copolymer of poly(oligo(ethylene glycol) methacrylate) (POEGMA) and poly(benzyl-L-aspartic acid) (PBLA) has been prepared by means of reversible addition–fragmentation chain transfer (RAFT) polymerization, ring-opening polymerization, and click reaction. After the PBLA segment was aminolyzed by the primary amine, N,N-diisopropylethylenediamine (DIEDA) and N,N-dibutylethylenediamine (DBEDA) with different ratios, amphiphilic block copolymers with various ionizable tertiary amines have been prepared, which can form stable micelles in neutral pH by self-assembly and disassembly under weak acidic conditions. After conjugation of Cy5.5/quencher to the copolymer, the as-formed micelles exhibit low fluorescence due to the Fluorescence Resonance Energy Transfer (FRET) between Cy5.5 and the quencher. However, an ultra-pH-sensitive fluorescence can be switched-on when it was in weak acidic conditions. The as-prepared nanoparticles have potential application in highly sensitive detection of the pH of tissues and cells, and can also work as a theranostic for cancer with drug delivery and imaging ability.
研究领域:
高分子化学
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纳米医学
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生物医学工程
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癌症研究
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2022
·
IF 9.224
Su Liu,Mingqiang Tang,Jun Pang,Jintao Hu,Weijie Chen,Jie Cheng,Zewei Liu,Haihong Zhao,Rong Tan
Water-soluble single-chain polymeric nanoparticles (SCPNs), which isolated catalytic sites within a hydrophobic interior, made water-incompatible organometallic catalysis highly efficient in water. However, it is still a great challenge to conveniently control their hydrophilicity, so as to combine reactivity and recovery of the catalytic SCPNs in aqueous systems. Herein, we have developed a series of catalytic SCPNs, which possessed CO2-switchable hydrophilic/hydrophobic behavior, to realize the gas-controlled reaction and separation for asymmetric sulfa-Michael addition (SMA) in water. A novel series of CO2-switchable random copolymers were thus synthesized by copolymerization of CO2-responsive amidine derivatives with hydrophobic chiral salen FeIII monomers via reversible addition-fragmentation chain transfer polymerization. Characterization suggested their CO2-controlled self-collapse behavior in water due to CO2-switched change in hydrophilicity/hydrophobicity of the amidine moiety. The resultant CO2-switchable SCPNs provided hydrophobic, catalytic compartments for asymmetric SMA in water upon CO2 addition, giving various chiral β-keto sulfides with almost quantitative yields (90–98%) and high enantioselectivities (93–99%). When CO2 is removed by N2 bubbling, they were collapsed and spontaneously precipitated from the aqueous system for steady reuse. The gas-controlled reaction-separation approach provides an energy-efficient way to combine reactivity and recovery of catalytic SCPNs in aqueous systems, which should be quite practical in large-scale industrial applications.
研究领域:
材料科学
·
化学工程
·
催化
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聚合物化学
·
纳米技术
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产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2022
·
IF 6.978
Jun Hu,Kunyan Lu,Chuan Gu,Xingyu Heng,Fangjian Shan,Gaojian Chen
The search for novel fluorescent materials has attracted the attention of many researchers. Numerous bioimaging materials based on the aggregation-induced emission (AIE) units have been surging and could be employed in wide areas during the past two decades. In recent few years, the appearance of nonconventional fluorescence emitters without aromatic conjugated structures provides another bioimaging candidate which has the advantage of enhanced biodegradability and relatively low cost, and their luminescent mechanism can be explained by clustering-triggered emission (CTE) like AIE. In our contribution, we utilize nonaromatic sugar as a monomer to prepare a series of glycopolymers with designed components through sunlight-induced reversible addition fragmentation chain transfer polymerization; these glycopolymers can be employed in bioimaging fields due to the bioactivity coming from sugar and CTE capacity.
研究领域:
材料科学
·
生物医学工程
·
化学
·
生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 36
·期 6
·681-685
·
IF 1.618
Liu Wenwen,Ma Ligang,Guo Zhuangzhuang,Liu Tao,Liu Yanju,Wang Dazhong,Kong Jinming
We propose a novel, ultrasensitive and low-cost sensor using reversible addition-fragmentation chain transfer (RAFT) polymerization as a signal amplification strategy for the detection of CYFRA 21-1 DNA fragment, a tumor marker of non-small cell lung carcinoma. The peptide nucleic acid (PNA) probes were firstly immobilized on magnetic beads (MBs) to capture the CYFRA 21-1 DNA specifically. After hybridization, CPAD was tethered to the hetero duplexes through carboxylate-Zr 4+ -phosphate chemistry. Subsequently, a number of fluorescent tags were introduced to the heteroduplexes through RAFT polymerization, leading to an amplification of the fluorescence signal. The sensor demonstrates a low limit of detection (LOD) of 0.02 fM. It has great selectivity with respect to base mismatch DNA, and high anti-interference ability in normal human serum. Overall findings of the study suggest that proposed sensor holds enormous potential to be used as a tool for the early-stage diagnosis of lung cancers.
研究领域:
生物技术
·
癌症诊断
·
纳米技术
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Junjun Chen,Zhenqiang Shi,Xijing Yang,Xiaoyu Zhang,Dongdong Wang,Shengxu Qian,Wenjing Sun,Cunli Wang,Qiongya Li,Zhengjian Wang,Yanling Song,Guangyan Qing
Blood infection can release toxic bacterial lipopolysaccharides (LPSs) into bloodstream, trigger a series of inflammatory reactions, and eventually lead to multiple organ dysfunction, irreversible shock, and even death, which seriously threatens human life and health. Herein, a functional block copolymer with excellent hemocompatibility is proposed to enable broad-spectrum clearance of LPSs from whole blood blindly before pathogen identification, facilitating timely rescue from sepsis. A dipeptide ligand of histidine–histidine (HH) was designed as the LPS binding unit, and poly[(trimethylamine N-oxide)-co-(histidine–histidine)], a functional block copolymer combining the LPS ligand of HH and a zwitterionic antifouling unit of trimethylamine N-oxide (TMAO), was then designed by reversible addition–fragmentation chain transfer (RAFT) polymerization. The functional polymer achieved effective clearance of LPSs from solutions and whole blood in a broad-spectrum manner and had good antifouling and anti-interference properties and hemocompatibility. The proposed functional dihistidine polymer provides a novel strategy for achieving broad-spectrum clearance of LPSs, with potential applications in clinical blood purification.
研究领域:
生物医学工程
·
材料科学
·
药学
·
血液学
·
传染病学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）