Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Qiming Zhang,Jiajun Hu,Chen Yang,Jixiang Li,Nan Liu,Wenshan Guo,Chaomeng Dai,Liang Wang,Yun Tian,Huu Hao Ngo
In this study, researchers successfully developed a novel Magnetic Molecularly Imprinted Polymer of Boronate Affinity Controllable-Oriented Dextran (MMIP-Dextran) to enhance membrane flux by selectively adsorbing polysaccharide-type substances. The structure and components of the MMIP-Dextran were thoroughly investigated using various techniques such as Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM), Brunauer-Emmett Teller (BET), Fourier Transform Infrared (FT-IR), and Thermogravimetric Analysis (TGA). Batch experiments were conducted to assess the adsorption performance and mechanisms. Results showed that the MMIP-Dextran exhibited a high adsorption capacity of 31.2 mg g −1 and good specific recognition performance (imprinting factor, IF=2.83) for the Dextran template. Furthermore, the MMIP-Dextran had a superior polysaccharide adsorption capacity of 22.10 mg g −1 compared to the Non-Imprinted Polymer (NIP) in soluble microbial products (SMPs) derived from a bioreactor , showing a 2.45-fold improvement. Meanwhile, the Specific Filtration Resistance (SFR) tests indicated that the membrane flux improvement by MMIP-Dextran (15.2 %) is 2.1 times better than NIP (7.2 %). Adsorption kinetics and thermodynamics analyses revealed that the spontaneous adsorption process was predominantly governed by chemically rate-limiting steps. Density Functional Theory (DFT) analysis further revealed that the spatial configuration transformation of the Boric Acid Ligand (BAL) played a crucial role in the adsorption/desorption process. Overall, this study offers a promising approach to promoting the permeate flux of membrane and provides a deeper understanding of the antifouling mechanism using MMIPs.
研究领域:
环境科学
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材料科学
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生物工程
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 19
·3066
·
IF 5.561
Xianghui Yan,Xiaofeng Gong,Zheling Zeng,Maomao Ma,Junxin Zhao,Jiaheng Xia,Meina Li,Yujing Yang,Ping Yu,Deming Gong,Dongman Wan
TheCinnamomum camphoraseed kernel (CCSK), with high contents of medium-chain oil (~59%) and protein (~19%), is an excellent source for a plant-based food ingredient. To broaden the application of the protein isolate (PI) from CCSK in the food industry, the Maillard reaction products (MRPs) were prepared by PI and dextran (DX) under mild wet-heating conditions (60 °C, 5 h), and the structural and functional properties of the PI-DX conjugates were investigated. The covalent bond between PI and DX was confirmed by the degree of grafting and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Compared with the heated PI, the PI-DX conjugates had more ordered structure, with the decreased random coil content. The changes in tertiary structure of PI-DX conjugates were reflected by the results of intrinsic fluorescence and surface hydrophobicity. Moreover, PI-DX conjugates showed better solubility, emulsifying properties, thermal stability and antioxidant activities. These results provided a theoretical basis for the development of PI-based MRPs with desirable characteristics.Keywords:plant protein;Maillard reaction;conjugate;structural property;functional property
研究领域:
食品科学
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蛋白质化学
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生物技术
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Yanmei Shi,Ronghua Tan,Chan Yu,Ying Wan
Image-guided chemo-photothermal therapy based on near-infrared (NIR) theranostic agents has found promising applications in treating tumors. In this multimodal treatment, it is of critical importance to image real-time distribution of photothermal agents in vivo and to monitor therapeutic outcomes for implementing personalized treatment. In this study, an optimally synthesized dextran-polylactide (DEX-PLA) copolymer was assembled with doxorubicin (DOX) and DiR, a kind of NIR dye, to construct desirable micelles ((DiR + DOX)/DEX-PLA) for performing image-guided chemo-photothermal therapy. These (DiR + DOX)/DEX-PLA micelles had good physical and photothermal stability in aqueous media and showed high photothermal efficiency in vivo. Based on the H22-tumor-bearing mouse model, (DiR + DOX)/DEX-PLA micelles were found to accumulate inside tumors sustainably and to emit strong fluorescence signals for more than three days. The (DiR + DOX)@DEX-PLA micelles together with NIR laser irradiation were able to highly inhibit tumor growth or even eradicate tumors with one injection and two dose-designated 5-minute laser irradiations at the tumor site during 14 days of treatment. Furthermore, they showed almost no impairment to the body of the treated mice. These (DiR + DOX)@DEX-PLA micelles have confirmative translational potential in clinical tumor therapy on account of their persistent image-guided capacity, high antitumor efficacy and good in vivo safety.
研究领域:
药剂学
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肿瘤学
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生物医学工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2025
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IF 5.2
Yunmei Li,Huilin Ma,Huan Shi,Biaobiao Wang,Desheng Li,He Tian,Xifan Mei,Chao Wu
Ulcerative colitis is an inflammatory disease that primarily involves intestinal inflammation and epithelial damage. The nano-targeted drug delivery system delivers drugs to the disease site, exerting effects such as inhibiting inflammatory response and reducing reactive oxygen species expression, thereby promoting recovery from ulcerative colitis. In this experiment, dextran sulfate-coated curcumin nanocrystals (NBD) were prepared for the oral treatment of ulcerative colitis (UC). NBD not only significantly enhances the water solubility and stability of curcumin but also possesses the ability of sustained release and targeting inflammatory macrophages. The sustained release effect of NBD was demonstrated by in vitro release experiments. In simulated gastric fluid, the cumulative release amount of NBD at 2 h was 21.99 ± 1.93 %, while in simulated colonic fluid, the cumulative release amount of NBD at 12 h was 84.98 ± 2.02 %. The ability of NBD to target inflammatory macrophages was verified through the transwell system, rat one-way intestinal perfusion experiment and in vivo imaging system. The in vitro and in vivo (mice) anti-inflammatory and antioxidant capacities of NBD were validated using immunofluorescence experiment, ELISA kits and reactive oxygen species-related detection kits. The results indicated that NBD could reduce inflammatory responses, promote macrophage polarization and inhibit oxidative stress. In addition, the therapeutic effect of NBD was further confirmed in this experiment by the clostridium perfringens-induced necrotizing enteritis model in chickens. In conclusion, NBD might be a potential pharmaceutical preparation for the treatment of UC.
研究领域:
药理学
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纳米医学
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消化病学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2018
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IF 2.441
Hao Wang,Honglei Chen,Jie Geng,Yi Zheng,Zhongyu Zhang,Lin Sun,Guihua Tai,Yifa Zhou
Dextran is a biocompatible glucose-based polymer that is widely used clinically as a plasma volume expander , anti-thrombotic agent, macromolecular carrier, peripheral blood flow enhancer, and artificial tears promoter. Because dextran has polydisperse molecular weights and tends to produce innumerable multi-charged precursor ions in the ion source, it is difficult to analysis this polymer using conventional liquid chromatography mass spectrometry . In this assay, all ion fragmentation strategy is used to solve this problem by allowing all dextran precursor ions generated in the ion source to enter the collision cell. Then serial dextran-specific fragments can be effectively generated from all precursor ions by higher energy collision dissociation and scanned by Orbitrap detector. These high resolution fragment ions provide high specificity and sensitivity for the quantitation of dextran in rat plasma. Here, we report a new quantitative method using size exclusion chromatography combined with Q Exactive mass spectrometry based on an all ion fragmentation strategy. Assay validation showed that this method is linear over the concentration range from 3 to 300 μg/mL. Our approach has been successfully applied to a pharmacokinetic study of dextran in rat and will promote the development of studies with other polysaccharides .
研究领域:
生物化学
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药理学
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分析化学
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2014
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IF 3.05
Liqiang Zou,Shengfeng Peng,Wei Liu,Xing Chen,Chengmei Liu
A novel delivery system was developed by coating dextran sulfate (DS) on the surface of amphiphilic chitosan derivative nanoliposome (DCMC-NL) through electrostatic interaction. DS coated DCMC-NL (DS-DCMC-NL) exhibited larger average particle size, lower polydispersity index, and more negative zeta potential, compared with DCMC-NL. DS-DCMC-NL exhibited good in vitro digestion stability, with no obvious variations on particle size under gastrointestinal tract environment, while DCMC-NL immediately formed sediment after being mixed with simulated intestinal fluid (SIF). EGCG as a hydrophilic model drug was encapsulated in DS-DCMC-NL, with a high encapsulation efficiency (90.8%). EGCG loaded DS-DCMC-NL (EG-DS-DCMC-NL) exhibited a relative good sustaining release property. The degradation of EGCG during SIF was effectively slowed by the encapsulation with DS-DCMC-NL. This study expects to provide a promising carrier for further application of EGCG.
研究领域:
食品科学
·
药剂学
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DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2015
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IF 3.088
Luyan Wu,Liping Zhang,Gang Shi,Caihua Ni
Zwitterionic nanoparticles have excellent serum stability. In this study, pH/redox responsive polymer was synthesized through a modification of dextran using succinic acid, followed by crosslinking with cystamine. The polymer could self-assemble into stable nanoparticles (NPs) in aqueous solution. The NPs carried certain amount of free carboxyl and amino groups on the surface, which endowed the NPs excellent anti-protein adsorption ability. The surface charge was negative at pH 7.4 and was converted to positive at pH 5.0. It was revealed that the NPs showed little non-specific protein adsorption and had excellent serum stability, and the NPs could be internalized in Hela cells rapidly. This result was ascribed to the charge reversible feature of the NPs. Doxorubicin (DOX) was loaded in the NPs for release studies in vitro. The DOX-loaded NPs exhibited obvious pH and reduction sensitivities in response to the environment in tumor cells due to the introduction of carboxyl groups, amino groups and disulfide bonds in the NPs. The NPs were biocompatible, biodegradable, and could be potentially applied as anticancer drug carriers for enhancement of tumor intercellular uptake of doxorubicin.
研究领域:
药剂学
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生物材料
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Li Wang,Xinbo Zhuang,Chenlinrui Yue,Hailu Liao,Yanchu Chen,Yinji Chen,Hongkai Xie
Scallops are renowned for their delicious taste and rich nutritional profile, including high contents of eicosapentaenoic acid and docosahexaenoic acid. However, they are highly susceptible to spoilage caused by lipid oxidation and microorganism proliferation. Therefore, in this study, trilayer films based on gelatin/gelatin-dextran/gelatin with different concentrations (0%, 0.3%, 0.6%, 0.9%, and 1.2%, w/w) of active fillers (citronellal and α-tocopherol) were developed to preserve scallop adductor muscle during cold storage. Scanning electron microscopy images showed that each layer was well-bonded with no gaps, indicating the successful preparation of the trilayer films. The addition of citronellal and α-tocopherol resulted in a porous cross-section with a slightly rough surface and increased hydrophobicity of the trilayer films. With increase in concentrations of citronellal and α-tocopherol, the thickness, opacity, antioxidant capability, and antibacterial activity of the trilayer films increased, while water vapor permeability and mechanical properties decreased. Both citronellal and α-tocopherol showed controlled release properties in four food simulants, with the longest release times in 95% (v/v) ethanol solution. Furthermore, the incorporation of 1.2% (w/w) of active fillers into the trilayer film significantly enhanced the chemical and microbiological stabilities of the scallop adductor muscles, thereby prolonging their shelf life by six days during cold storage. The developed trilayer films showed excellent controlled release, antibacterial properties, and antioxidant capabilities, indicating their potential for effective preservation of aquatic food products.
研究领域:
食品科学
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材料科学
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生物技术
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产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2024
·
IF 3.7
Yun Liu,Ao Huang,Xiaowei Wen
Phospholipase D (PLD) is essential for the bioconversion of phosphatidylcholine (PC) to phosphatidylserine (PS), a process valuable in functional food and medicine. This study explores the stability and catalytic properties of PLD immobilized on chitosan-encapsulated magnetic nanoparticles (CMNPs), utilizing oxidized dextran (DX) and glutaraldehyde (Glu) as cross-linkers. The cross-linker concentration and immobilization time were optimized to assess their effects on PLD catalytic performance. PLD immobilized on CMNPs with DX (DX-CMNPs-PLD) exhibited optimal activity at pH 8.0 and 30 °C, retaining over 40 % activity after 14 cycles, while Glu-cross-linked PLD (Glu-CMNPs-PLD) retained approximately 65 %. DX-CMNPs-PLD demonstrated superior pH, temperature, and operational stability compared to free PLD. Additionally, the immobilized PLD was characterized using transmission electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. Kinetics parameters (V max and K m ) of the immobilized PLD were also studied with free PLD serving as a control. Conformational analyses indicated a significant change in PLD's secondary structure, particularly in β-sheet content, which likely contributed to the enhanced stability and activity. These findings suggest a promising approach for PLD immobilization on CMNPs, with notable implications for biotechnological applications.
研究领域:
生物技术
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食品科学
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医学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 15
·5692-5701
·
IF 3.288
Hailing Han,Ying Zhang,Shunzi Jin,Ping Chen,Shi Liu,Zhigang Xie,Xiabin Jing,Zhanfeng Wang
Gliomas are the most common malignant brain tumors and notorious due to the blood–brain barrier (BBB). In this study, an RVG peptide is used as a targeting ligand to construct multi-targeting micelles (RVG–Dex–PTX) towards the brain (passing the BBB) and brain glioma. In vivo imaging and fluorescence imaging were carried out. The results showed that Cy5-labeled M(RVG, PTX) can cross the BBB and reach the intracranial C6 glioma, prolonging the median mean survival time. Specifically, the elevated RVG29 could enhance the uptake of the micelles by C6 glioma cells and thus enhance the PTX efficacy. In conclusion, RVG29 is a promising targeting peptide for the BBB and intracranial tumors, and the M(RVG, PTX) micelles prepared from dextran, PTX, and RVG29 are a potential formulation for C6 glioma treatment.
研究领域:
神经肿瘤学
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纳米医学
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药剂学
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癌症研究
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
QiHong Wu,RongRong Jin,Ting Feng,Li Liu,Li Yang,YuHong Tao,James M Anderson,Hua Ai,Hong Li
Superparamagnetic iron oxide nanoparticles have been widely used in biomedical applications, but understanding of their interactions with the biological immune system is relatively limited. This work is focused on dextran-coated iron oxide nanoparticles and their induced autophagy in human monocytes. We found that these nanoparticles can be taken up by human monocytes, followed by localization within vesicles or free in cytoplasm. Autophagosome formation was observed with increased expression of LC3II protein, the specific marker of autophagy. The autophagy substrate p62 was degraded in a dose-dependent manner, and autophagy was blocked by autophagy (or lysosome) inhibitors alone or along with iron oxide nanoparticles, indicating that autophagosome accumulation was mainly due to autophagy induction, rather than blockade of autophagy flux. Interestingly, iron oxide nanoparticles increased the viability of human monocytes, but the mechanism was not clear. We further found that inhibition of autophagy mostly attenuated the survival of cells, with acceleration of the inflammation induced by these nanoparticles. Taken together, autophagic activation in human monocytes may play a protective role against the cytotoxicity of iron oxide nanoparticles.
研究领域:
纳米技术
·
免疫学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Food Frontiers
·
2023
·
IF 9.9
Chenlinrui Yue,Fengjiao Fan,Yinji Chen,Yong Fang,Hongkai Xie
Biodegradable active packaging films with antimicrobial and antioxidant functions have received much attention due to the food safety and plastic pollution issues. This study prepared and characterized a novel bioactive binary composite film based on gelatin (Ge) and dextran (Dx) incorporated with various concentrations (0%, 0.3%, 0.6%, 0.9%, 1.2%, w/w) of citronellal (Cit) and α-tocopherol (Toc). The thickness, L *, b *, total color difference, water contact angle, opacity, and crystallinity of film significantly increased, whereas the film solubility, swelling degree, water vapor permeability, tensile strength, elongation at break, and a * of film significantly decreased with the increase of Cit and Toc concentrations. However, the addition of Cit and Toc had no significant effect on the film's moisture content and thermal stability. Fourier transforms infrared spectral pattern indicated that physical interactions (H-bonding) between biopolymer matrix and active fillers were formed. Scanning electron microscope images showed that the film added with active fillers had a rough surface and a spongelike cross-section structure compared to control film. Moreover, the release curves of Cit and Toc showed that the film in 95% ethanol solution had the longest release time. Furthermore, the film added with Cit and Toc effectively inhibited the growth of pathogenic and spoilage bacteria ( Shewanella putrefaciens and Escherichia coli ) and quenched the free radicals (2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)). Overall, it can be inferred that the Ge–Dx-based composite film incorporated with Cit and Toc is a potential antibacterial and antioxidant biodegradable material for food packaging applications to maintain food quality.
研究领域:
材料科学
·
食品科学
·
生物技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）