PLANT SCIENCE
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2025
·
IF 4.1
Ruilan Ran,Xiaofeng Li,Jiwei Zhang,Jiecai Zhao,Xin Zhao,Xiaoyun Cui,Guoxiong Chen,Pengshan Zhao
Sand rice ( Agriophyllum squarrosum (L.) Moq.), a species of Amaranthaceae sensu lato , is an annual psammophyte found mainly on sand dunes in arid and semi-arid regions of Central Asia. Trichomes on the leaf surface are a key adaptive feature of sand rice, however, their ecophysiological functions remain to be elucidated. In this study, the anatomical analysis of a leaf reveals that the foliage is characterized by its isobilateral and amphistomatic nature. Together with an average δ 13 C value of −28.57 ‰, these findings indicate that sand rice is a C 3 plant. Utilizing a trichome-defective mutant ( Agriophyllum squarrosum trichomeless1 , astcl1 ) identified from an EMS mutagenesis library by our group, alongside the wild type Shapotou (SPT), we observed that both epidermal surfaces on SPT leaves exhibit dense and dendritic trichomes, forming rough leaf boundary layers. Chlorophyll leaching analysis revealed that SPT has a higher epidermal permeability, with 86.32 % of chlorophylls leaching out within 30 minutes, compared to only 24.18 % for astcl1 . The astcl1 leaf displayed a higher leaf wettability, lower water loss, and thicker cuticle compared to SPT leaves, which may be associated with trichomes serving as the primary pathway for nonstomatal water movement. This is supported by the observation that toluidine blue staining is more intense in trichomes than in pavement cells. Our findings reveal that the correlation between trichomes and the cuticle appears to be a critical factor in managing water loss and overall plant hydration, highlighting the significant role of trichomes in the adaptation of sand rice to desert environments.
研究领域:
植物学
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生态学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 5
·2701
·
IF 4.9
Shizi Zhang,Yunfeng Xu,Fang Wang,Liyun Yang,Lijuan Luo,Lingyan Jiang
Anthracnose caused byColletotrichum gloeosporioidesis a destructive disease ofStylosanthes(stylo). Combination treatment of phloretin and pterostilbene (PP) has been previously shown to effectively inhibit the conidial germination and mycelial growth ofC. gloeosporioidesin vitro. In this study, the effects of PP treatment on the growth ofC. gloeosporioidesin vivo and the biocontrol mechanisms were investigated. We found that exogenous PP treatment could limit the growth ofC. gloeosporioidesand alleviate the damage of anthracnose in stylo. Comparative transcriptome analysis revealed that 565 genes were up-regulated and 239 genes were down-regulated upon PP treatment during the infection byC. gloeosporioides. The differentially expressed genes were mainly related to oxidative stress and chloroplast organization. Further physiological analysis revealed that application of PP afterC. gloeosporioidesinoculation significantly reduced the accumulation of O2•−level and increased the accumulation of antioxidants (glutathione, ascorbic acid and flavonoids) as well as the enzyme activity of total antioxidant capacity, superoxide dismutase, catalase, glutathione reductase, peroxidase and ascorbate peroxidase. PP also reduced the decline of chlorophyll a + b and increased the content of carotenoid in response toC. gloeosporioidesinfection. These results suggest that PP treatment alleviates anthracnose by improving antioxidant capacity and reducing the damage of chloroplasts, providing insights into the biocontrol mechanisms of PP on the stylo against anthracnose.Keywords:phloretin;pterostilbene;ROS scavenging;chloroplasts;anthracnose;stylo
研究领域:
植物病理学
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分子生物学
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植物生理学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Aiping Li,Chen Wang,Yunfeng Chai,Zhiming Huang,Binbin Yao,Chengyin Lu,Miao Yu,Hongping Chen
Validamycin A (VA) and spinosyn A (SA) have been utilized as antibiotic pesticides in tea plantations for the control of pests and diseases. Both pesticides pose significant risks to human health and have been listed as key controlled new pollutants in China. So far, there have been few reports for the risk assessment of antibiotic pesticides in tea. In this study, a hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) method was developed for the simultaneous detection of VA and SA residues in tea. Method validation demonstrated good accuracy and precision. During tea planting, SA and VA in tea shoots degraded fast, with the half-life of 0.36 to 0.43 days for SA and 0.72 to 0.92 days for VA. SA and VA in tea shoots degraded fast initially and then followed by a slower phase. Both SA and VA degraded rapidly during the withering and drying stages. Compared to green tea, the degradation rates of both pesticides were faster during black tea manufacturing. Transfer rates of VA and SA from tea into infusions had transfer rates of 76.7 % to 99.5 % and 1.5 % to 2.7 %, respectively. The chronic risk quotient (RQc) < 1 for both antibiotic pesticides indicated negligible consumer risk from tea.
研究领域:
农业科学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2024
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IF 4.1
Bowen Zha,Xinhui Huang,Liangbin Lin,Hongyu Guo,Fafu Yang
Although some detection methods for folic acid have been developed based on the conventional instruments, the rapid, simple and in-situ detection for folic acid was rarely reported. In this work, a “turn-on” and “color-changing” fluorescence sensor ( TBCS ) on detecting folic acid was designed and synthesized. TBCS emits the green fluorescence, which transferred to the strong blue fluorescence after binding folic acid. The high sensing selectivity was observed among various ions and biomolecules. The detection limitation of TBCS for folic acid was 4.32 × 10 −7 M. The sensing mechanism was proposed as that the ICT effectiveness of TBCS was interrupted by sensing folic acid. The application experiments on test paper, folic acid tablets and simulated sample displayed the good in-situ detecting ability of TBCS for folic acid, suggesting the good application prospect on rapid, simple and in-situ detecting folic acid in real environments.
研究领域:
分析化学
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生物医学
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DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2025
·Vol. 2025
·期 1
·8616106
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IF 4.2
Chen Chen,Zengrong Ye,Jianan Li,Na Liu,Bo Wang,Guoying Zhou
Rheum tanguticum petiole juice, widely consumed in Tibet as both a vegetable and an herb, exhibits varying chemical compositions depending on the production methods. This study aims to identify the bioactive components in raw juice using ultra-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry (UPLC–QTOF–MS–MS) and analyze their antidiabetic properties through network pharmacology. In addition, the effects of the three types of membrane clarification were evaluated. A total of 36 compounds were identified, and a components–targets–pathways–disease network was constructed for diabetes treatment. Among the membranes tested, the 0.2 μm microfiltration membrane exhibited the highest permeate flux and retained significant amounts of total flavonoids, phenolics, and proanthocyanidins, making it the optimal choice for juice clarification. These findings lay the groundwork for the industrial processing and utilization of R . tanguticum petiole juice in China.
研究领域:
植物化学
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分析化学
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药理学
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食品科学
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膜技术
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糖尿病研究
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产品关联
DOI
高影响因子
Water
·
2024
·Vol. 16
·期 9
·1295
·
IF 3
Hualong He,Sulin Cai,Siyuan Chen,Qiang Li,Yunchao Luo,Xiaoyi Zeng,Rumeng Ye,Pengwei Wan,Xingjun Tian
This study investigates the combined effect of microplastics and cadmium on the decomposition of litter, the structure of fungal communities, and the feeding behavior of invertebrates in an aquatic ecosystem. Through a series of microcosm experiments, we demonstrate that exposure to MPs and Cd significantly reduced the decomposition of leaf litter. Notably, the cumulative impact of combined MP and Cd exposure was found to be greater than their individual effects. During this process, the carbon–nitrogen ratio of the litter increased, while dehydrogenase activity and fungal biomass were inhibited. Additionally, the relative abundance of Ascomycota and Basidiomycota fungi decreased, weakening their role in the decomposition of leaf litter. Conversely, MPs and Cd reduced the relative content of leaf litter lignin, improving its quality as food, thereby leading to an increase in the feeding rate of invertebrates. This dual effect indicates that micropollutants suppress the decomposition of litter by regulating microbial metabolic activity and fungal community structure but promote invertebrate feeding. Our findings provide crucial insights into the adverse effects of MPs and Cd on the structure and diversity of aquatic fungal communities, which could have long-term impacts on the food webs and nutrient cycling progress of aquatic ecosystems.
研究领域:
水生生态学
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环境科学
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微生物学
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生态毒理学
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 22
·3691
·
IF 4.7
Haicui Suo,Jitao Liu,Li Wang,Chengchen Li,Jianwei Shan,Kang An,Kun Yang,Xiaobo Li
Individual carotenoids provide significant health benefits to humans, and potatoes are recognized as some of the most stable crops, distinguished by their substantial carotenoid content. To elucidate the accumulation patterns of individual carotenoids in potatoes, we quantified the carotenoid content in yellow- and white-fleshed genotypes across five developmental stages using LC-MS/MS. A total of 22 carotenoids were identified in yellow potatoes, whereas 18 were detected in white potatoes. The yellow-fleshed genotype was characterized by high levels of individual carotenoids and xanthophyll esters, with violaxanthin as the dominant component. The white-fleshed genotype exhibited low concentrations of individual carotenoids and xanthophyll esters, with lutein as the predominant compound. Notably, lutein, violaxanthin, zeaxanthin, antheraxanthin, neoxanthin, violaxanthin myristate, and lutein myristate were consistently detected during the developmental period in both genotypes. Violaxanthin myristate was identified as the dominant xanthophyll ester in both genotypes, showing an increasing trend throughout the tuber development stages. In contrast, xanthophyll esters maintained stable low levels in white-fleshed potatoes while exhibiting increasing types and contents in yellow-fleshed potatoes as the tubers continued to grow. Additionally, violaxanthin exhibited a significant correlation with b*, H°, and C*, suggesting that it may play an important role in forming yellow flesh.
研究领域:
农学
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营养科学
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植物生理学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 12
·2805-2813
·
IF 3.637
Li Wang,Zhang Xiaoyue,Miao Chunyue,Li Ruijun,Ji Yibing
Paper-based devices have been very much in the foreground of analytical science recently. This work innovatively proposed a fluorescent paper–based sensor (FPS) constructed on a hybrid polydimethylsiloxane (PDMS)/paper platform where cellulose papers functionalized with carbon dots (CDs) as fluorophores by Schiff base chemistry were loaded on the grooves array of a designed PDMS plate. As a proof of concept, the performance of FPS was investigated with folic acid (FA) as the target analyte. Under optimal conditions, FPS enabled a rapid fluorescence quenching response to FA via inner filter effect in a wide range of 1–300 μmol L −1 with the limit of detection of 0.28 μmol L −1 . The feasibility of FPS was further verified by the detection of FA in orange juice and urine samples with satisfactory results. The covalent modification of CDs on paper endowed the FPS with good assay reproducibility and stability. Interestingly, FPS achieved a more sensitive assay of FA than the conventional strategy, by which the same CDs were directly used to detect FA in a solution-based system. The FPS illuminated a novel strategy for construction of reliable and sensitive assays based on paper-based devices. It is of paramount importance for its practical application in biosensing and clinical diagnosis.
Graphical abstract
研究领域:
分析化学
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生物传感
·
临床诊断
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DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 15
·2842
·
IF 3.06
Li Duan,Chenmeng Zhang,Chenjing Zhang,Zijing Xue,Yuguang Zheng,Long Guo
TheArtemisia argyileaf (AL) has been used as a traditional medicine and food supplement in China and other Asian countries for hundreds of years. Phytochemical studies disclosed that AL contains various bioactive constituents. Among bioactive constituents, phenolic acids have been recognized as the main active compounds in AL. To the best of our knowledge, no research has been focused on extraction method for the bioactive phenolic acids from AL. Nowadays, deep eutectic solvents (DESs) are emerging as a new type of green and sustainable solvent for efficient extraction of bioactive compounds from natural products. In the present study, an environmentally friendly extraction method based on DESs was established to extract bioactive phenolic acids from ALs. Diverse tailor-made solvents, including binary and ternary DESs, were explored for simultaneous extraction of four phenolic acids (3-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid) from AL. The results indicated that the ternary DES composed of a 2:1:2 molar ratio of choline chloride, malic acid, and urea showed enhanced extraction yields for phenolic acids compared with conventional organic solvents and other DESs. Subsequently, the extraction parameters for the four phenolic acids by selected tailor-made DESs, including liquid–solid ratios, water content (%) in the DESs, and extraction time, were optimized using response surface methodology and the optimal extraction conditions were: extraction time, 23.5 min; liquid–solid ratio, 57.5 mL/g (mL of DES/g dry weight of plant material); water content, 54%. The research indicated that DESs were efficient and sustainable green extraction solvents for extraction of bioactive phenolic acids from natural products. Compared to the conventional organic solvents, the DESs have a great potential as possible alternatives to those organic solvents in health-related areas such as food and pharmaceuticals.Keywords:deep eutectic solvents;Artemisia argyileaves;phenolic acids;extraction;response surface methodology
研究领域:
植物化学
·
药学
·
食品科学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Hong-Kai Xie,Ao Li,Man-Tong Zhao,Min Zhang,Zhong-Yuan Liu,Fa-Wen Yin,Xiao-Yang Liu,Da-Yong Zhou,Fereidoon Shahidi,Bei-Wei Zhu
Phospholipids (PLs), the main lipid component in marine shellfish, consist mainly of glycerophosphocholine (GPC) and glycerophosphoethanolamine (GPE). This work compared the oxidative susceptibility of GPC and GPE in dried shellfish during storage and also investigated the effects of natural phenolic antioxidant on such oxidative susceptibility. The content of four representative polyunsaturated fatty acids (C18:3 n-3, C20:4 n-6, C20:5 n-3, and C22:6 n-3) esterified onto GPC or GPE in dried scallop adductor muscle during storage at two temperatures were matched with first-order kinetic models, in order to ascertain the oxidation reaction rate constant ( k ). The results showed that GPE possessed greater oxidation rate than GPC during storage, and GPC could be more effectively protected by the antioxidants of bamboo leaves (AOB, polar polyphenolic antioxidants) compared with GPE. This study provides a practical method for accurate evaluation of oxidative susceptibility of different PL classes in complex food systems.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
Heliyon
·
2023
·
IF 4
Chuan Jiang,Chaonan Wang,Weizhong Qu,Yuanyuan Wang,Hua Wang,Xin Wei,Mingyan Wang,Qianqian He,Yihan Wang,Lirong Yuan,Yonggang Gao
Depression is a complex and prevalent mental illness. Cherry leaf is a traditional Chinese herbal medicine, which has confirmed to exert a certain antidepressant effect, but its potential neural regulation mechanism is not clear. This paper aims to investigate the improved action of cherry leaf decoction (CLD) on chronic unpredictable mild stress (CUMS) rats and its potential neural regulation mechanism by verifying the role and function of NMDAR regulatory target α2δ-1 in depression due to CUMS. Male SD rats were subjected to random stressors persisting for 5 weeks to establish the CUMS depression rat model. CLD could effectively alleviate depression-like behaviors of CUMS rats in behavioral tests including sucrose preference test, forced swimming test, tail suspension test and open field test. After the administration of the CLD, the expression of corticotropic-releasing hormone (CRH) in the hypothalamus was inhibited. Moreover, the levels of CRH, adrenal cortical hormone (ACTH) and corticosterone (CORT) in serum also decreased significantly. CUMS upregulated the expressions of α2δ-1, N-methyl- d -aspartate receptor 1 (NR1), NR2A and NR2B, and enhanced the binding ability to of α2δ-1 and NR1, which were reversed by CLD. The results demonstrated that CLD could ameliorate depression-like behaviors due to CUMS, which was related to the fact that CLD down-regulated α2δ-1 level and interfered with α2δ-1 binding to NR1, thereby reducing NMDAR expression and ultimately inhibiting HPA axis activity.
研究领域:
神经科学
·
中药学
·
精神医学
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2011
·
IF 2.089
Hengde Li,Tianfei Zhang,Weiqin Fu,Kazuhiro Tamura
Liquid–liquid equilibrium (LLE) data of the binodal curves and tie-line compositions were examined for ternary mixtures of (water + methanol + geraniol) and (water + ethanol + geraniol) at T = (283.15, 298.15, and 313.15) K. The immiscible area of the (water + methanol + geraniol) system is wider than that for the ethanol system at the same temperature. The experimental liquid–liquid equilibrium data have been represented by using the original UNIQUAC, extended UNIQUAC, and modified UNIQUAC models.
研究领域:
化学工程
·
物理化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）