JOURNAL OF NANOPARTICLE RESEARCH
·
2014
·Vol. 16
·期 11
·1-13
·
IF 2.278
Liu Wenjuan,Yao Jun,Cai Minmin,Chai Hankuai,Zhang Chi,Sun Jingjing,Chandankere Radhika,Masakorala Kanaji
A new nanopesticide, carboxymethyl-β-cyclodextrin-Fe 3 O 4 magnetic nanoparticles-Diuron (CM-β-CD-MNPs-Diuron), was synthesized from an inclusion complex of CM-β-CD-MNPs as host and diuron as guest molecules. The transmission electron microscopy revealed it had an average diameter of 25 nm which is more or less the same as that of MNPs (average diameter ~23 nm). The CM-β-CD grafting was confirmed by infrared spectroscopy, and the amount of CM-β-CD grafted on the surface of MNPs was determined to be 144.1 mg/g by thermogravimetry. The feasibility of using CM-β-CD-MNPs as a nanocarrier for loading diuron was verified by investigating the formation of inclusion complex. The complexation of CM-β-CD-MNPs with diuron followed the Langmuir adsorption isotherm. In this work, the potential toxic effect of CM-β-CD-MNPs-Diuron on soil microbial was evaluated by microcalorimetry, urease enzyme and real-time quantitative PCR (qPCR). The thermokinetic parameters were observed to decrease with increase in the loading of CM-β-CD-MNPs-Diuron in soil. The urease activity data showed that there was a significant effect ( p < 0.05) of CM-β-CD-MNPs-Diuron on the enzyme activity. The microcalorimetric analysis was in agreement with qPCR, confirming the toxic effect of this nanopesticide on microorganism in soil.
研究领域:
环境科学
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微生物学
·
纳米技术
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产品关联
DOI
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2021
·
IF 1.751
Yu Zhang,Yong-Hang Li,Shi-Bin Jiang,Shi-Ling Wang,Shao-Song Qian,Jie Qin,Jian-Ping Ma,Juan Li
Reactions of 2-{[5-(Pyridin-2-yl)-4H-1,2,4-triazol-3-yl]sulfanyl}acetic acid (H2L) with manganese(II), zinc(II) and cadmium(II) acetate in CH3OH/H2O mixture result in the corresponding metal complexes. All the obtained complexes were characterized by IR spectroscopy, elemental analyses, ESI-MS, and single-crystal X-ray diffraction. Structural analyses reveal the dinuclear complexes with the compositions [Mn2L2(CH3OH)2(H2O)2]·2CH3OH (1), [Zn2L2(CH3OH)2(H2O)2]·2CH3OH (2), and 1D chain structure [CdL(H2O)]n (3) in all complexes, H2L is doubly deprotonated (L2-) and serves as equilibrium anion. The carboxylate acts as monodentate ligand in 1 and 2, While μ1,1-bridging ligand in 3. The measurement of Jack Bean urease inhibitory activity showed that 1 and 3 exhibited considerable inhibitory effects for Jack Bean urease with IC50 Values of 12.4 and 2.7 μM, respectively. In addition, bioactive complexes 1 and 3 can effectively bind to bovine serum albumin (BSA), which was assessed by tryptophan emission quenching measurement
研究领域:
配位化学
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生物无机化学
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药物化学
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DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2018
·
IF 3.466
Qi Liu,Wei-Wei Ni,Zhen Li,Cai-Fu Bai,Dan-Dan Tan,Chang-Jun Pu,Dong Zhou,Qing-Peng Tian,Ni Luo,Kai-Li Tan,Le Dai,Yuan Yan,Yong Pei,Xian-Hui Li,Zhu-Ping Xiao,Hai-Liang Zhu
The continuing emergence of drug-resistant Helicobacter pylori (HP) drives the ongoing need for the development of new and effective anti-HP drugs. Urease inhibitor has now gained strong interest as an alternative approach for HP infections . 3-Chlorophenyl-3-hydroxypropionylhydroxamic acid (CPH), a novel urease inhibitor identified in our group, shows impressive potency, which was optically separated for a further exploration. Here, we report in vitro / in vivo pharmacological evaluation of (±)-CPHs and the enantiomers. The raceme and the individual enantiomers significantly suppress gastritis at 32 mg/kg b.i.d dose with lower toxicity to mammalian cells (with CC 50 s ≥ 3.16 mM) and mice (LD 50 s ≥ 2338 mg/kg) than the clinically used agent acetohydroxamic acid . Furthermore, a significant increase of eradication of HP is observed for the combination of (±)-CPHs or the enantiomers with an antimicrobial. These studies revealed that CPH is a promising candidate for an alternative treatment of HP-dependent conditions by targeting virulence factor urease, and CPH may be used as a raceme.
研究领域:
药理学
·
微生物学
·
感染病学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 2
·1-8
·
IF 5.7
Li Shenghua,Xiao Lingyu,Xiao Longqian,Tan Hongliang
The potential of coordination polymers (CPs) as a host of integrating multiple guest species to construct a fluorescence resonance energy transfer (FRET) nanoprobe was demonstrated. The ZnCPs built from zinc(II) and adenine was employed as a model of CPs to integrate carbon dot (CD) and phenol red (PR) for producing the FRET nanoprobe (CD/PR@ZnCPs). Benefiting from the confinement effect of ZnCPs, the integrated CD and PR can be brought in close proximity to favor the occurrence of FRET process from CD to PR, which leads to the quenching of CD fluorescence. However, the FRET process was disrupted upon the red-shift of PR absorption from 428 to 562 nm in alkaline medium, and consequently switches on the fluorescence of CD/PR@ZnCPs. Based on this finding, by utilizing urease to hydrolyze urea and mediate medium pH, a turn-on fluorescent method was established for the detection of urease activity. This fluorescent method has a linear response that covers 5 to 150 U/L urease with a detection limit of 0.74 U/L and exhibits an excellent selectivity over other enzymes. The successful determination of urease in saliva samples demonstrates the applicability of the fluorescent nanoprobe in complex biological matrix. Graphical Abstract
研究领域:
分析化学
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生物传感器
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纳米技术
·
酶学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Jialin Sai,Lu Zhou,Lin Jiang,Dongguo Xue,Renjun Pei,Aihua Liu,Lijun Xu
Signal amplification techniques are highly desirable for the analysis of low-level targets that are closely related with diseases and the monitoring of important biological processes. However, it is still challenging to achieve this goal in a facile and economical way. Herein, we developed a novel dual signal amplification strategy by combining urease catalysis with the release of Ag+ from silver nanoparticles (AgNPs). This strategy was used for quantifying a DNA sequence (HIV-1) related with human immunodeficiency virus (HIV). DNA target HIV-1 hybridizes with the capture DNA probe on magnetic beads and the reporter DNA probe on AgNPs, forming a sandwich complex. The captured AgNPs are then transformed into numerous Ag+ ions that inactivate numerous ureases. Without catalytic production of ammonia from urea, the substrate solution shows a low pH 5.8 that will increase otherwise. The pH change is monitored by a pH indicator (phenol red), which allows for colorimetric detection. The proposed approach is sensitive, easy to use, economic, and universal, exhibiting a low detection limit of 9.7 fM (i.e., 1.94 attomoles) and a dynamic linear range of 4 orders for HIV-1 sequence detection.
研究领域:
分析化学
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生物化学
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纳米技术
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病毒学
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DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
·
2014
·
IF 2.041
Yong-Peng Xu,Jie Qin,Shi-Min Sun,Tong-Tong Liu,Xiao-Lin Zhang,Shao-Song Qian,Hai-Liang Zhu
Three novel complexes, [Ni II (dpp) 2 (L) 2 ] ( 1 ), [Ni II (eda) 2 (L) 2 ] ( 2 ) and [Ni II (deda) 2 (L) 2 ] ( 3 ) (L = 3-pyridinyl-4-amino-5-mercapto-1,2,4-triazole, dpp = 1,3-diaminopropane, eda = ethanediamine, deda = N,N-dimethyl ethylenediamine), were synthesized by reacting 3-pyridinyl-4-amino-5-mercapto-1,2,4-triazole with diamines and nickel(II) salt. The complexes were structurally determined by single-crystal X-ray diffraction. The inhibitory activity was tested in vitro against jack bean urease. Molecular docking was investigated to insert complexes into the crystal structure of jack bean urease at the active site to determine the probable binding mode. The experimental values and docking simulation exhibited that complexes 1 , 2 , 3 had better inhibitory activity than the positive reference acetohydroxamic acid, showing IC 50 values of 48.16, 32.35 and 15.22 μM, respectively. These complexes exhibited inhibitory activities as potent urease inhibitor.
研究领域:
配位化学
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生物无机化学
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药物化学
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产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Zhixiang Zhu,Yuanjing Zou,Ling Ou,Meiyun Chen,Yujiang Pang,Hui Li,Yajie Hao,Bingmei Su,Yuqian Lai,Liping Zhang,Junwei Jia,Ruixia Wei,Guimin Zhang,Meicun Yao,Zhong Feng
Background: Triphala, is a composite of three individual botanical drugs: Terminalia chebula, Terminalia bellirica, and Emblica officinalis. It exhibits properties such as heatclearing, anti-inflammatory, anti-fatigue, antioxidant, and antibacterial effects,making it extensively utilized in India and Tibet. It has been found to exhibitinhibitory effects on Helicobacter pylori (H. pylori); however, further comprehensive research is still needed to elucidate its specific antibacterial mechanism. The present study investigates the in vitro antibacterial activity and antibacterial mechanism of Triphala against H. pylori.Methods: Ours research investigates the in vitro inhibitory activity of Triphala on multiple standard and clinical strains using microdilution broth method, time-kill curve, time-bactericidal curve and scanning electron microscopy (SEM). Furthermore, the antibacterial mechanism of Triphala is further explored through experiments on urease activity, biofilm formation, anti-adhesion properties, virulence actor assays using RT-qPCR and Western Blotting techniques.Results: The research findings indicate that Triphala exhibits a minimum inhibitory concentration of 80–320 μg/mL against both standard and clinical strains of H. pylori. Triphala exerts its anti-H. pylori effect by perturbing the microstructure of H. pylori, downregulating adhesion-associated genes (alpA, alpB, babA), urease-related genes (ureA, ureB, ureE, ureF), and flagellar genes (flaA, flaB); inhibiting bacterial adhesion, biofilm formation, urease activity as well as CagA protein expression.Discussion: These findings suggest that Triphala exerts inhibitory effects on H. pylori activity through multiple mechanisms, underscoring its potential as a new drug for the prevention and treatment of H. pylori infection.
研究领域:
药理学
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微生物学
·
传统医学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 10
·1-10
·
IF 5.3
Wang Yuhan,Zhang Ping,Zhou Chuxian,Zheng Zicong,Quan Qiang,He Guangbo,Zhai Hening,Song Qifang,Zhang Xiaoli,Li Heng,Peng Lanhuan,Liao Yuqin,Fang Wei,Li Minmin,Liang Jiajie,Tang Yong
Helicobacter pylori ( H. pylori ) is a major pathogen associated with gastric ulcers and gastric cancer, infecting approximately half of the global population. Therefore, developing sensitive, rapid, and cost-effective detection methods for H. pylori is critical for public health. We developed an innovative microscale colorimetric microsensor integrated with smartphone ambient-light sensors for portable, on-site quantification of H. pylori- associated urease activity in dental plaque. This low-cost device (< $ 5 fabrication) employs 3D-printed components and a custom app to measure transmittance changes from urease-catalyzed urea hydrolysis with phenol red, achieving a robust correlation with commercial microplate reader absorbance ( R 2 = 0.9989). The system demonstrates exceptional Analytical sensitivity, with limits of detection of 9.75 U/L for urease And 1.709 × 10 3 CFU/mL for H. pylori , alongside high reproducibility (CV < 10%) And stability over 14 days. When applied to the on-site detection of dental plaque among volunteers, the smartphone-based colorimetric microsensor exhibited detection results that were highly aligned with H. pylori infections confirmed by the urea breath test. It outperformed commercial test strips And achieved 100% sensitivity, 95.5% specificity, And 97.1% accuracy. This miniaturized, smartphone-based platform offers a promising solution for home self-testing and demonstrates significant potential for enhancing H. pylori preliminary screening at the community level.
Graphical abstract
研究领域:
微生物学
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消化病学
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分子诊断
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即时检测
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生物传感
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口腔微生物组
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转化医学
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产品关联
DOI
PubMed
高影响因子
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
·
2023
·
IF 5.6
Liangliang Cheng,Wanlu Zheng,Ya-Nan Zhang,Xuegang Li,Yong Zhao
A highly sensitive fiber-optic urea sensor was designed and experimentally demonstrated based on surface plasmon resonance (SPR) effect and metal-organic zeolite framework (MOF), [zeolite imidazole skeleton-8 (ZIF-8)]/urease modification. The SPR effect was excited by plating gold film on the thin core fiber (TCF) in multimode-thin core-multimode fiber (MMF) structure, which behaved high refractive index sensitivity. The ZIF-8/urease composite film was grown on the gold film by rapid aqueous phase synthesis method, in which the ZIF-8 could immobilize more urease and made urease and urea fully contact. Since the urease catalyzed urea hydrolysis would change the refractive index, the measurement of urea concentration was realized with high sensitivity and good selectivity. Experimental results showed that the urea measurement sensitivity of the proposed sensor could reach 6 nm/mM within the concentration range of 1–7 mM, which has great application potential in the field of human health monitoring.
研究领域:
光学传感
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生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2016
·
IF 1.78
Zhi-Jian Chen,Ya-Na Chen,Chun-Na Xu,Shan-Shan Zhao,Qi-Yue Cao,Shao-Song Qian,Jie Qin,Hai-Liang Zhu
Three novel mononuclear complexes, [M Ⅱ ( L ) 2 ·2H 2 O], (M = Cu, Ni or Cd; HL = 4-(3,4-dichlorophenyl)piperazine-1-carboxylic acid)were synthesized and structurally determined by single-crystal X-ray diffraction. Molecular docking study preliminarily revealed that complex 1 had potential urease inhibitory activity. In accordance with the result of calculation, in vitro tests of the inhibitory activities of complexes 1–3 against jack bean urease showed complex 1 (IC 50 = 8.17 ± 0.91 μ M) had better inhibitory activities than the positive reference acetohydroxamic acid (AHA) (IC 50 = 26.99 ± 1.43 μ M), while complexes 2 and 3 showed no inhibitory activities., kinetics study was carried out to explore the mechanism of the inhibiting of the enzyme , and the result indicated that complex 1 was a competitive inhibitor of urease. Albumin binding experiment and in vitro toxicity evaluation of complex 1 were implemented to explore its Pharmacological properties .
研究领域:
生物无机化学
·
药物化学
·
酶学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 10
·期 14
·2584-2596
·
IF 6.331
Qin Yao,Binghai Chen,Jiaxiang Bai,Wenbo He,Xu Chen,Dechun Geng,Guoqing Pan
Urinary tract infection (UTI) represents one of the most common nosocomial infections, which is mainly related to indwelling catheters or stents. In addition to the formation of biofilms to reduce antibiotic sensitivity, the urease-producing bacteria can also increase urine pH, causing Ca2+ and Mg2+ deposition and finally catheter obstruction. The prevention of UTIs and its complication (i.e., encrustation) thus is a great challenge in design of catheters and ureteral stents. In this work, a metal-catechol-assisted mussel chemistry (i.e., dopamine self-polymerization) was employed for surface functionalization of commercially available catheters with antimicrobial peptides (AMP), for the purpose of long-term anti-infection and encrustation prevention. To improve the stability of the polydopamine coating on polymeric stents, we used Cu2+-coordinated dopamine self-polymerization. Then, a cysteine-terminated AMP was introduced on the polydopamine coating through Michael addition. We found that the Cu2+-coordinated polydopamine coating showed improved stability and antibacterial effect. The cytotoxicity test confirmed that the bioinspired antibacterial coating showed good biocompatibility and no obvious toxicity. The results confirmed that the stents with AMP could in situ inhibit bacterial growth and biofilm formation, and finally reduce the deposition of struvite and hydroxyapatite crystals both in vitro and in vivo. We anticipate that this bioinspired strategy would develop a safe, stable and effective antibacterial coating on urinary tract medical devices for long-term bacterial inhibition and encrustation prevention.
研究领域:
生物材料
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泌尿外科
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感染病学
·
表面工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jing Zhao,Shibo Cheng,Xin Wang,Ruilian Yu,Bin Du,Jiajun Tong,Yiwei Liu,Taiping Qing,Xiaofeng Liu
In different contexts of Alzheimer's disease (AD), specific criteria for detecting toxic soluble amyloid β (Aβ) oligomers are crucial: Early detection requires highly-sensitive and accurate technology, while assessing treatment efficacy requires qualitative analysis using predefined thresholds. However, traditional methods, particularly single signal output, struggle to fulfill these criteria. Here, we present a urease-mediated homogeneous dual-mode biosensor that combines colorimetry and field-effect transistor (FET) through dual-aptamer recognition for the analysis of Aβ oligomers. This approach overcomes the challenges associated with surface modification of FET biosensors, allowing for both qualitative on-site detection (at pM levels) and highly-sensitive quantitative analysis (at fM levels) of Aβ oligomers in undiluted samples. The dual-mode biosensor displayed excellent performance in fetal bovine serum (FBS), artificial cerebrospinal fluid (aCSF) and rabbit whole blood samples, achieving a high recovery rate ranging from 96.1 % to 101 % and a low relative standard deviation between 1.7 % and 5.1 % when detecting Aβ oligomers in undiluted samples. Furthermore, clinical tests of serum and plasma samples from AD patients and healthy individuals also verified the sensitivity and specificity of the proposed dual-mode biosensor, demonstrating its potential application in early screening and monitoring of AD treatment processes.
研究领域:
生物传感器
·
神经退行性疾病
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分析化学
·
生物医学工程
·
阿尔茨海默病研究
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）