Current Research in Food Science
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2026
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IF 7.7
Donglei Zheng,Mengle Li,Ying Qian,Xiangsong Chen,Jianglin Chen,Long Jin,Lili Cao,Min Pang,Jiaying Huo
Sialic acid (Neu5Ac) possesses a wide range of bioactivities; however, its instability under acidic conditions prevents it from effectively reaching the intestine. This underscores the urgent need to develop a stable delivery system capable of protecting Neu5Ac from gastric degradation and achieving its controlled release in the intestine. To enhance the stability and achieve controlled release, Neu5Ac was encapsulated within water-in-oil-in-water (W1/O/W2) emulsion-based microcapsules in this work. S2 emulsions featured a “dual-chamber, tri-phase” structure, comprising a Neu5Ac-chitosan fluid gel (inner aqueous phase), maize oil (oil phase), and an external aqueous phase of 13% sodium starch octenyl succinate, 5% β-cyclodextrin, 1.5% sodium caseinate, and 0.5% carboxymethyl cellulose. Microstructural analyses confirmed spherical morphology with core-shell architecture and bimodal droplet distribution (1-10 μm). In addition, S2 emulsions exhibited an encapsulation efficiency (EE) of 92.44%, a zeta potential of −60.80 mV, a Sauter mean diameter (d 3 , 2 ) of 1.337 μm, shear-thinning behavior and weak gel network stability. S2 emulsions were spray-dried to obtain S2 microcapsules. S2 microcapsules exhibited an EE of 89.43% and a d 3 , 2 of 3.839 μm, while FT-IR and XRD verified physical encapsulation without covalent bonding. In vitro digestion, S2 microcapsules exhibited controlled release behavior during simulated intestinal digestion, with a Neu5Ac release rate of 85.61% after 120 min. In mice, microencapsulated Neu5Ac increased the relative abundance of Akkermansia , Lactobacillus , and Bifidobacterium . This system demonstrates promising potential for the co-delivery and controlled release of Neu5Ac, and shows great promise for expanding its applications in the food industry.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Bohan Zhang,Jinying Lin,Wenzheng Wu,Zhenglong Chen,Ruiyun You,Yudong Lu,Tao Zhang,Yong Fan
A hydrogel SERS platform requiring no pretreatment has been developed. • The LOD for DOX and MTO were as low as 3.4 × 10 −8 M and 3.6 × 10 −9 M. • Machine learning achieved a 100% identification for DOX and MTO plasma samples. Rapid quantitative detection and classification of trace drugs directly in complex biological fluids are crucial in modern analytical chemistry. This paper proposes a label-free, interference-resistant smart surface-enhanced Raman scattering (SERS) sensing strategy. A machine learning-assisted Au@Ag/C-CNF/PEGDA hydrogel SERS sensor is developed for monitoring and identifying anthracycline anticancer drugs in plasma. Addressing the susceptibility to contamination and oxidation inherent in conventional SERS sensors, this sensor leverages the pore-trapping effect and nanoparticle encapsulation properties of hydrogel. This approach not only eliminates macromolecular interference but also maintains a high signal output of 71.05% for the internal Au@Ag SERS probe after 50 days. Leveraging the electrostatic interactions of carboxylated cellulose (C-CNF), the sensor exhibits excellent signal reproducibility with detection limits as low as 3.4 × 10 −8 M for doxorubicin (DOX) and 3.6 × 10 −9 M for mitoxantrone (MTO). Furthermore, this study employed machine learning models for high-dimensional feature extraction of SERS data, achieving up to 100% classification accuracy for DOX and MTO in spiked plasma samples. This approach holds promise as a robust solution for clinical drug concentration monitoring and species identification, potentially advancing precision treatment strategies in clinical practice. Download: Download high-res image (183KB) Download: Download full-size image
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Qi Liu,Wenrou Yu,Anping Liu,Lianjie Zhou,Wen Tang,Yingzhou Huang
Multiple Synergistic SERS Enhancement of 3D micro-nano Ni-foam@MoS₂ • Overcomes EBC matrix interference for ultrasensitive 2.95 nM lactate detection • AI algorithms decode complex SERS spectra for non-invasive health monitoring. To address the critical demand for highly sensitive sensing interfaces in non-invasive exhaled breath condensate (EBC) analysis, this study develops a high-performance SERS substrate featuring interlaced, needle-like MoS₂ nanoclusters grown in situ on a 3D nickel foam scaffold (Ni-foam@MoS₂). This architecture effectively overcomes the severe signal heterogeneity and poor wearable compatibility that typically plague traditional plasmonic metal substrates. The system synergizes multiple coupled SERS enhancement mechanisms: the 3D porous scaffold and interwoven needle-like arrays form abundant optical microcavities. These microcavities effectively confine incident light and significantly enhance light-matter interactions, thereby inducing electromagnetic enhancement. Concurrently, the interlaced MoS₂ arrays not only efficiently concentrate trace analytes but also induce substantial chemical mechanism via vigorous charge transfer. Consequently, the substrate achieves an outstanding average enhancement factor of 1.88 × 10 9 , alongside superb signal reproducibility and long-term environmental stability. By deploying this Ni-foam@MoS₂ substrate for the rapid off-line analysis of simulated exhaled breath condensate (EBC), we achieved the ultrasensitive detection of lactate (LA), a critical metabolic biomarker, with a limit of detection as low as 2.95 nM. Furthermore, coupling these complex spectral datasets with advanced artificial intelligence algorithms enabled the highly accurate classification of concentration-dependent LA spectral variations, demonstrating significant proof-of-concept potential for mask-integrated biomolecular sensing and non-invasive physiological monitoring.
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ANALYTICAL BIOCHEMISTRY
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2026
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IF 3.1
Yanfeng Wang,Yuzhen Wang,Peng Fei,Duoliang Shan
A simple and sensitive method was developed for the electrochemiluminescence (ECL) determination of DNA. • Au-luminol NPs addressed the issues of weak solubility and ECL signals of luminol in neutral conditions. • The target DNA induced the formation of heme and G-quadruplexes as biomimetic enzymes, thereby enhancing the electrochemical luminescence intensity of luminol. A novel signal amplification approach was developed for DNA detection, leveraging the synergistic effect of G-quadruplex/hemin DNAzyme and gold nanoparticles (Au NPs) to enhance the electrochemiluminescence (ECL) of luminol. The optimized system exhibited excellent linear response toward target DNA with a linear detection range from 10 fM-50 pM and achieved fM-level DNA detection. The spiked recoveries of serum samples ranged from 95.1% to 107.6%, demonstrating its reliable performance for practical sample analysis. Compared with recently reported analogous ECL sensors for DNA detection, this system featured a wider linear range, lower limit of detection and stronger anti-matrix interference capability, offering a new strategy for rapid and ultrasensitive detection of target analytes in complex matrices. Download: Download high-res image (235KB) Download: Download full-size image
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Advanced Sensor and Energy Materials
·
2026
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IF 12.3
Tingting Wang,Hao Song,Xiaohui Gong,Zongyu Han,Taoming Xu,Yanan Xu,Dan Zhao,Chuanxia Chen,Yizhong Lu,Jian Sun
Biothiol analysis is crucial for evaluating redox homeostasis and monitoring various physiological processes. However, conventional fluorescence-based strategies often rely on structurally complex probes and lack straightforward multi-target discriminative capabilities. Herein, we present a versatile, pre-synthesis-free fluorogenic platform driven by the target-responsive inhibition of in-situ polymer dot (PD) formation. We discovered that dopamine (DA) spontaneously condenses with N -[3-(trimethoxysilyl)propyl]diethylenetriamine (AEEA) to yield robustly fluorescent PDs. Biothiols, acting as highly reactive competitors, intercept the DA precursor via a preferential Michael addition, fundamentally suppressing PD formation and inducing a marked fluorescence decrease. This mechanism enables the rapid, highly sensitive quantification of glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), with low limits of detection (LODs) of 0.095, 0.115, and 0.083 μM, respectively. Significantly, the introduction of a structurally distinct DA-DAMO analog facilitated the discrimination of these biothiols. This reaction-interference strategy was then successfully coupled with enzymatic cascades to evaluate glutathione reductase (GR) activity within 20 min, achieving a LOD of 0.046 mU/mL. The programmable analyte-dependent fluorescence outputs were successfully employed as signal readouts to construct molecular logic gates. This work establishes target-regulated in-situ nanomaterial formation as a powerful paradigm for biothiol sensing, enzyme assays, and advanced logic operations.
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BIOELECTROCHEMISTRY
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2026
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IF 4.9
Xiangli Ru,Ke Guo,Xuelian Li,Guanglei Ma,Lele Wei,Zhenning Fu,Yuming Guo,Xiaoming Ma,Lin Yang
Overcoming the inherent limitations of electron transmembrane transfer is a challenge faced by cell-based biosensors. Exploiting conductive nanomaterials to modify cell membranes offers a promising strategy to address this issue. Herein, we report a hybrid system consisting of multiwall carbon nanotubes (MWCNTs) integrated with red blood cells (RBCs), where the MWCNTs serve as artificial electrical conduits across the cell membrane to facilitate electron transmembrane transfer. Consequently, the MWCNT-engineered RBCs (RBC@MWCNTs) hybrids exhibit significantly enhanced electrochemical activity, enabling highly sensitive and selective simultaneous detection of coexisting essential biomolecules dopamine (DA) and uric acid (UA), both of which are involved in diverse physiological processes and pathological pathways. The electrochemical platform achieves the linear detection ranges of 4–400 μM for DA and 2–500 μM for UA, with corresponding detection limits of 0.10 μM and 0.68 μM, respectively. Furthermore, the successful application of RBC@MWCNTs hybrids for the detection of DA and UA in real biological serum samples validates the effectiveness of this membrane modification strategy in overcoming electron transfer limitations for practical bioelectrochemical sensing applications.
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npj Flexible Electronics
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2026
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IF 15.4
Lin Kanglong,Yang Baili,Yan Guizhi,Li Changhao,Zhao Guoxu,Zou Yang,Li Zhou,Wang Dong
Chronic diabetic wounds present a significant clinical challenge due to antibiotic abuse and limited understanding of the wound microenvironment. Real-time monitoring of key biomarkers in the wound microenvironment can provide critical insights into wound status and guide therapeutic interventions. In this study, we developed an intelligent wound patch by integrating a near-infrared (NIR)-responsive photothermal antibiotic-releasing hydrogel (PPQTA) with a multichannel electrochemical biosensor array, which takes sensor monitoring-guided precise drug administration as its core. The sensing module can continuously monitor the key biomarkers in the wound in situ with high sensitivity and strong anti-interference capability within the physiological range (pH: 4–9; uric acid: 0–700 μM; glucose: 5–30 mM), providing a quantitative basis for drug release. The PPQTA hydrogel can release drugs on demand via NIR irradiation based on the sensing results, achieving 100% antibacterial efficacy against both Gram-negative and Gram-positive bacteria. Experiments in a diabetic rat wound model confirmed that this patch can monitor the dynamic changes of biomarkers in situ and guide drug administration within 7 days. After terminating drug administration on day 4, the bacteria in the wound were effectively eliminated on day 5, and the wound healing process was significantly promoted. By combining real-time diagnostics with responsive therapy, this patch offers a closed-loop strategy for personalized management of diabetic chronic wounds.
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LUMINESCENCE
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2026
·Vol. 41
·期 7
·e70592
·
IF 2.8
Lanlan Wu,Xiaochun Deng,Jiyang Liu
The detection of metabolites, such as uric acid (UA), is crucial for diagnosing and monitoring various metabolic disorders. Herein, an electrochemiluminescence (ECL) sensor for uric acid (UA) detection was constructed via integrating a cascade of bioenzyme (urate oxidase [UOx]) and confined nanozyme reactions. Gold nanoparticles (AuNPs) were confined within amino‐functionalized vertical mesoporous silica film (NH 2 ‐VMSF) via electrochemical deposition. The NH 2 ‐VMSF was synthesized on indium tin oxide (ITO) through electrochemical‐assisted self‐assembly (EASA) method. Amino groups on the NH 2 ‐VMSF surface served as anchoring sites for AuNPs, which were electrochemically reduced and confined within the nanochannels. The confined AuNPs exhibited strong pseudo‐peroxidase‐like activity, boosting ECL response of luminol‐H 2 O 2 . Compared to the NH 2 ‐VMSF/ITO electrode, the AuNPs‐deposited electrode produced a 3.4‐fold increase in ECL intensity. Urate oxidase catalyzed the oxidation of uric acid, producing H 2 O 2 , which acted as a co‐reactant for luminol, enabling indirect uric acid detection. The sensor demonstrated a detection range of 0.1–20 μM with a low limit of detection (LOD, 85 nM). The sensor was used for UA detection in complex biological samples (urine). The nanoconfined AuNPs provided a stable and effective environment for nanozyme‐catalyzed reactions, ensuring high sensitivity and long‐term stability. A cascade electrochemiluminescence sensor integrating urate oxidase catalysis with nanochannel‐confined AuNPs nanozyme enables highly sensitive and selective detection of uric acid. The confined AuNPs exhibit strong peroxidase‐like activity, amplifying the luminol‐H 2 O 2 signal by 3.3‐fold and achieving a low limit of detection.
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Frontiers in Nutrition
·
2026
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IF 5.5
Bingye Xu,Xiaoxiao Li,Rong Liu,Ying Han,Pan Zhen,Hanyue Fu,Huan Lv,Fan Wei,Haoxin Cui,Bowei Zhang,Shuo Wang
IntroductionHyperuricemia (HUA) is a major risk factor for gout and renal injury, underscoring the need for safe and effective natural dietary interventions. This study investigated the chemical composition and antihyperuricemic effects of dealcoholized Zhuyeqing liquor extract (ZLE) using both cellular and animal models.MethodsThe nutritional composition and phytochemical profile of ZLE were characterized by quantitative analysis and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The antihyperuricemic activity of ZLE was evaluated in adenosine-induced HepG2 cells and in a hypoxanthine/potassium oxonate-induced mouse model of HUA by assessing uric acid (UA) metabolism, hepatorenal function, urate transporter expression, and renal inflammatory signaling.ResultsQuantitative analysis showed that ZLE is rich in polysaccharides, terpenes, and flavonoids. UPLC–MS/MS identified 389 phytochemical constituents, predominantly iridoid glycosides, organic acids, and flavonoids. In adenosine-induced HepG2 cells, ZLE significantly reduced UA production and xanthine oxidase (XOD) activity. In HUA mice, ZLE dose-dependently decreased serum UA levels, improved renal and hepatic function by reducing serum creatinine, blood urea nitrogen, alanine aminotransferase, and aspartate aminotransferase levels, and inhibited hepatic XOD activity. Furthermore, ZLE restored renal urate homeostasis through coordinated regulation of key urate transporters and alleviated HUA-induced renal inflammation and histopathological injury by suppressing the lipopolysaccharide-mediated NLRP3/Caspase-1 signaling pathway.DiscussionThese findings demonstrate that ZLE exerts antihyperuricemic and renoprotective effects through coordinated modulation of uric acid production, renal urate transport, and inflammatory signaling. The results support the potential application of ZLE as a functional food ingredient for dietary management of HUA and provide a scientific basis for translating traditional dietary resources into modern nutritional interventions.
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BIOCHEMICAL PHARMACOLOGY
·
2026
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IF 6.5
Cheng Cao,Mubin Sun,Jiabao Li,Zhenyu Yuan,Yuwei Gu,Yufan Shao,Lisheng Wang,Xiaohua Jin,Yijie Cheng,Bo Fan
Urate transporter 1 (URAT1, encoded by SLC22A12 ) is a key mediator of renal urate reabsorption and an important pharmacological target for urate-related disorders. Sodium butyrate (BuNa), a gut microbiota derived short-chain fatty acid, has been implicated in host metabolic regulation, but its role in renal urate transport remains unclear. Here, using molecular docking, cellular thermal shift assays, immunoprecipitation-mass spectrometry and multi-omics analyses, we identified free fatty acid receptor 2 (FFAR2) as a direct target of BuNa in renal proximal tubular HK2 cells . BuNa binding upregulated FFAR2 and promoted URAT1 expression and urate uptake via an FFAR2-dependent pathway. Mechanistically, BuNa/FFAR2 signaling acts via GNAQ to activate PLCβ, generating diacylglycerol and engaging a PKC-dependent p38 MAPK/ELK1 cascade that upregulates arrestin beta 2 (ARRB2). ARRB2 directly binds to IκB, preventing its phosphorylation-dependent degradation, thereby stabilizing IκB and inhibiting NF-κB nuclear translocation. Within the SLC22A12 promoter, NF-κB (RELA/p50) and hepatocyte nuclear factor 1 beta (HNF1β) compete for overlapping binding sites. By blocking NF-κB nuclear entry, BuNa relieves this competition, permitting HNF1β-driven SLC22A12 transcription. Thus, BuNa enhances URAT1-mediated urate reabsorption in HK2 cells via an FFAR2/GNAQ/p38 MAPK/ELK1/ARRB2 axis that stabilizes IκB, suppresses NF-κB, and relieves the competitive binding between NF-κB and HNF1β on the SLC22A12 promoter. These findings establish a previously unrecognized gut microbiota–kidney axis linking microbial BuNa to renal urate homeostasis through a novel transcriptional regulatory circuit.
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Journal of Bioresources and Bioproducts
·
2025
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IF 13
Qian Zhang,Hui’e Jiang,Zhijian Li,Lijuan Chen,Fengqian Yang,Jiamin Zhang,Bo Zhang,Xinhua Liu
Uric acid (UA) level is a pivotal clinical human-health biomarker providing predictive feedback for multitudinous well-known kidney, cardiovascular and metabolic syndrome diseases. Off-the-shelf UA detection methods clinically rely on uricase suffer from limitations such as high costs, longstanding result acquisition, circumscribed testing locations, rigorous expertise requirements, and difficulty in home-detecting due to serum testing systems. Here, inspired by the pH-paper, a scaleable, rapid, non-invasive/-enzymatic/-serodiagnostic, and home-detecting “abnormal UA alarm” platform for UA detection in saliva was developed by strategically integrating the proposed paper-based fluorescent sensing-materials (NIFP-SM) with a user-orientated intelligent red-green-blue (RGB) analysis device. Therefore, NIFP-SM is nano-engineered through straightforward interfacial interactions of functional building blocks of on-demand naphthyl imide-derived fluorescent self-assembled micro-particles (NIFS) with lamellar structure and commercially-used filter paper. The NIFS possesses dominantly wide detection range (0–5 000 µmol/L) and high sensitivity (limit of detection = 0.91 µmol/L). Surprisingly, NIFS exhibited outstanding identifiability for uric acid even in the presence of 16 interferents, substantiating accurate detection-capability in intricate environments. Thus NIFP-SM equipped with NIFS resoundingly achieved efficient, rapid, and on-site visual detection of UA in saliva, urine-simulants, and foods. Further, the NIFP-SM-based automatic analysis platform integrated with an intelligent RGB analysis device was manufactured and enabled accurate quantitative, low-cost, non-invasive/-enzymatic/-serodiagnostic, rapid, home-detecting for UA, eliminating the need for costly equipment and specialized personnel and thereby facilitating early-warning detection of abnormal UA-levels associated diseases.
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Advanced Healthcare Materials
·
2025
·
IF 9.6
Yuxuan Lin,Zhenglin Zhu,Zhengjiang Xu,Junkang Chen,Zhiqiang Li,Hui Huang,Yuan Zhang,Di Chen,Bo Liang,Guocheng Wang
Acute gout, characterized by recurrent inflammatory flares caused by monosodium urate (MSU) crystal deposition, presents a therapeutic challenge due to the limited efficacy of current drugs in concurrently eliminating MSU crystals and resolving inflammation. To overcome this, a manganese-based nanozyme derived from a ZIF-8 metal-organic framework (MOF) is developed, which is designed to avoid the reliance on precious metals seen in existing urate oxidase-mimetic systems. By leveraging competitive coordination between zinc and manganese during MOF synthesis, atomic-level dispersion of manganese active sites with precise modulation of the Mn 4+ /Mn 2+ redox equilibrium is achieved. Coupled with optimized pyrrolic/graphitic nitrogen configurations, the nanozyme demonstrates triple enzymatic activities: urate oxidase-like activity for uric acid degradation (preventing MSU crystallization), catalase-like activity for H 2 O 2 scavenging, and superoxide dismutase-like activity to alleviate oxidative stress. Notably, the nanozyme directly destabilizes preformed MSU crystals via surface charge modulation and lattice disruption. In an acute gout model, the nanozyme surpasses colchicine in accelerating MSU clearance—through simultaneous dissolution of existing crystals and inhibition of new formation—while reducing inflammatory cytokine levels without systemic toxicity. This work introduces a redox-adaptive nanozyme design strategy for managing crystalopathies, providing a dual-action solution to combat both MSU-driven inflammation and crystallization.
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