TETRAHEDRON
·
2021
·
IF 2.457
Peixin Niu,Jiaojiao Liu,Yifan Rong,Xingjiang Liu,Liuhe Wei
This work proposes a fluorescent probe that uses coumarin as a chromophore and NBD as a sensing unit to detect hydrogen sulfide . The probe can work well in aqueous media, and can quickly respond to hydrogen sulfide. When detecting hydrogen sulfide, the probe has high selectivity and good anti-interference ability. Besides, in monitoring hydrogen sulfide in living cells and zebrafish, good results have demonstrated the probe’s potential in practical applications.
研究领域:
生物化学
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医学检测
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分子生物学
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DOI
高影响因子
Biomicrofluidics
·
2015
·Vol. 9
·期 2
·
IF 3.357
Li Yinbao,Yang Xiujuan,Chen Zuanguang,Zhang Beibei,Pan Jianbin,Li Xinchun,Yang Fan,Sun Duanping
Investigations were conducted to determine acute effects of Pb 2+ and Cu 2+ presented individually and collectively on zebrafish embryos. Aquatic safety testing requires a cheap, fast, and highly efficient platform for real-time evaluation of single and mixture of metal toxicity. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic effects of Pb 2+ and Cu 2+ using zebrafish ( Danio rerio ) embryos. The microfluidic chip is composed of a disc-shaped concentration gradient generator and 24 culture chambers, which can generate one blank solution, seven mixture concentrations, and eight single concentrations for each metal solution, thus enabling the assessment of zebrafish embryos. To test the accuracy of this new chip platform, we have examined the toxicity and teratogenicity of Pb 2+ and Cu 2+ on embryos. The individual and combined impact of Pb 2+ and Cu 2+ on zebrafish embryonic development was quantitatively assessed by recording a series of physiological indicators, such as spontaneous motion at 22 hours post fertilization (hpf), mortality at 24 hpf, heartbeat and body length at 96 hpf, etc. It was found that Pb 2+ or Cu 2+ could induce deformity and cardiovascular toxicity in zebrafish embryos and the mixture could induce more severe toxicity. This chip is a multiplexed testing apparatus that allows for the examination of toxicity and teratogenicity for substances and it also can be used as a potentially cost-effective and rapid aquatic safety assessment tool.
研究领域:
毒理学
·
环境科学
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生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2018
·Vol. 13
·期 18
·2611-2618
·
IF 3.692
Huan Feng,Qingtao Meng,Yue Wang,Chengchen Duan,Cuiping Wang,Hongmin Jia,Zhiqiang Zhang,Run Zhang
Graphical Sensing and imaging of hypochlorous acid (HOCl) : A new responsive fluorescence probe, PQI , is reported for quantitative fluorescence detection of HOCl in aqueous solution and live organisms. Visualisation of HOCl in live cells and in vivo sensing and imaging of HOCl in zebrafish and nude mice were achieved by using PQI as the fluorescence probe. The development of effective bioanalytical methods for rapid, sensitive and specific detection of HOCl in vitro and in vivo plays a key role for better understanding the roles of this molecule in normal and diseased conditions, but remains challenging due to the highly reactive nature of HOCl and the complicated biological conditions. In this work, a new fluorescence probe, PQI , was developed for monitoring of the HOCl level in biological samples. PQI was easily synthesized by a one-step condensation reaction. Upon addition of HOCl, significant changes in the absorption spectra and the color of the solution were noticed, facilitating the “naked eye” detection of HOCl in PBS buffer. The fluorescence of PQI was found to be significantly increased within a few seconds, leading to “OFF-ON” fluorescence response towards HOCl. The sensing mechanism, oxidation of thioether by HOCl, was confirmed by HRMS titration analysis. PQI features a large Stokes shift, high sensitivity and selectivity, and rapid fluorescence response towards HOCl. Quantitative detection of HOCl in single live cells was demonstrated through fluorescence imaging and flow cytometry analysis. PQI was then successfully used in visualisation of HOCl in live zebrafish and nude mice.
研究领域:
生物化学
·
分析化学
·
医学影像
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF APPLIED TOXICOLOGY
·
2024
·
IF 2.7
Boyang Xu,Jun Yan,Yangtao Zhou,Feng Zhang,Binjie Wang,Jiye Wang,Yuanzhao Wu,Yu Xu
As global regulations on synthetic cannabinoids tighten, illicit vendors increasingly turn to new structures of synthetic cannabinoids to evade legal scrutiny. MDA-19, a novel synthetic cannabinoid, exhibited significant agonistic effects on type 2 cannabinoid receptors in vivo and showed emerging trends of abuse in illicit markets. However, research on the toxicological effects of MDA-19 remains scarce. In this study, we examined the effects of MDA-19 on neurodevelopment, behavior, oxidative stress, and metabolomics by exposing zebrafish embryos to MDA-19 solutions with concentrations of 1, 10, and 20 mg/L over 5 days. Results revealed that exposure to 10 and 20 mg/L of MDA-19 accelerated hatching in zebrafish embryos but led to reduced body length without affecting mortality or malformation. Furthermore, exposure to all concentrations of MDA-19 resulted in diminished swimming ability and reduced activity time in zebrafish. Transgenic zebrafish ( hb9 -GFP) exposed to MDA-19 exhibited impaired development of spinal motor neurons. Notably, exposure to 20 mg/L MDA-19 increased the levels of reactive oxygen species (ROS) in zebrafish and elevated the activity of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT), while the levels of the lipid oxidation product malondialdehyde (MDA) remained unaffected. Nontargeted metabolomics analyses showed that MDA-19 interfered with multiple metabolic pathways affecting energy metabolism, such as alanine, aspartate, and glutamate metabolism; the citric acid cycle (TCA cycle), pantothenate, and coenzyme A biosynthesis; and purine metabolism. In conclusion, the present study provided the essential evidence for the neurotoxic effects of MDA-19, which was associated with impaired neurodevelopment, dysregulation of oxidative stress homeostasis, and altered energy metabolism.
研究领域:
毒理学
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神经生物学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Yanan Sun,Ping Ding,Jiayi Zhang,Kexin Sun,Xintong Li,Qing Ge,Yao Dang,Yunjiang Yu,Guocheng Hu
Microplastics (MPs) pollution, together with its consequential effect on aquatic biota, represent a burgeoning environmental concern that has garnered significant scholarly attention. Thiamethoxam (TMX), a prevalently utilized neonicotinoid insecticide, is renowned for its neurotoxic impact and selective action against targeted pests. The aquatic environment serves as a receptacle for numerous pollutants, such as MPs and neonicotinoid insecticides. However, there is currently a lack of comprehensive understanding regarding the toxic effects of co-exposure to aged MPs and neonicotinoid insecticides in aquatic organisms. Therefore, we endeavor to elucidate the deleterious impacts of aged polystyrene (PS) and TMX on zebrafish ( Danio rerio ) larvae when present at environmentally relevant concentrations, and to reveal the underlying molecular mechanisms driving these effects. Our study showed that exposure to aged PS, TMX, or their combination notably inhibited the heart rate and locomotion of zebrafish larvae, with a pronounced effect observed under combined exposure. Aged PS and TMX were found to diminish the activity of antioxidative enzymes (SOD, CAT, and GST), elevate MDA levels, and disrupt neurotransmitter homeostasis (5-HT, GABA and ACh). Notably, the mixtures exhibited synergistic effects. Moreover, gene expression related to oxidative stress (e.g., gstr1, gpx1a, sod1, cat1, p38a, ho-1, and nrf2b ) and neurotransmission (e.g., ache, ChAT, gat1, gabra1, 5ht1b, and 5ht1aa ) was significantly altered upon co-exposure to aged PS and TMX in larval zebrafish. In summary, our findings support the harmful effects of aged MPs and the neonicotinoid insecticides they carry on aquatic organisms. Results from this study enhance our understanding of the biological risks of MPs and insecticides, as well as help fill existing knowledge gaps on neonicotinoid insecticides and MPs coexistence toxicity in aquatic environment.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biology-Basel
·
2025
·Vol. 14
·期 1
·51
·
IF 3.5
Ferry Saputra,Tzu-Ming Tseng,Franelyne P. Casuga,Yu-Heng Lai,Chih-Hsin Hung,Chung-Der Hsiao
Simple SummaryMany studies have been developed to calculate the blood flow velocity in adult zebrafish. However, the current method requires high-cost equipment a lot of training before it can be replicated. In this study, we provide a simple, cheap, and non-invasive method to enable the measurement of zebrafish blood flow velocity. By applying the same method as the one previously developed for larvae zebrafish, blood flow velocity can be measured and its sensitivity validated by performing an experiment under several ambient temperature conditions, as well as the presence of a toxicant. The applicability of this method was also checked by applying the same method to other fish. Overall, this method provides an easy, cheap, and robust method that can be used by low- to medium-scale laboratories to contribute to knowledge in the relevant fields.Blood flow is an important physiological endpoint to measure cardiovascular performance in animals. Because of their innate transparent bodies, zebrafish is an excellent animal model for assessing in vivo cardiovascular performance. Previously, various helpful methods for measuring blood flow in zebrafish larvae were discovered and developed. However, an optimized method to measure blood flow in adult zebrafish has not been reported. In this paper, the tail fin region was selected as target for blood flow measurements using the Trackmate method, provided by ImageJ platform. Based on power statistic calculations, the aortic vessel at the tail base was selected, and other parameters, such as ambient temperature, were investigated for method standardization, in order to minimize experimental variation. The method was also validated using fenpropathrin and ponatinib, which showed some cardiac alterations in a previous zebrafish study. We also checked the versatility of this method by following the same setup in black tetra and medaka and found that this method performed well. However, our results show that heavy pigmentation, like that found in tiger barb, and overlapping vessels, like those in parrot fish, make it hard for this method to perform well. Overall, an optimized protocol was used for the first time to measure blood flow velocity in adult wild-type zebrafish without the aid of transgenic lines or fluorescent dye.
研究领域:
生物学
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医学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Bin Li,Chenglong Sun,Yanan Yang,Chenqinyao Li,Te Zheng,Jiashuo Zhou,Yun Zhang
This study focuses on the potential hazards of borneol (BO) to aquatic organisms and human health. BO has antibacterial, anti-inflammatory and antioxidant activities, and is widely used in medicine, cosmetics, and detergents. In this study, zebrafish was used as a model organism to systematically evaluate the effects of BO on the heart, liver, kidney, and nervous system. The effects of BO on metabolites of zebrafish were studied using MALDI-MSI. The results showed that a high concentration of BO (500 μM) could induce morphological abnormalities (swim-bladder loss, spinal curvature, body-length shortening), cardiotoxicity (decreased heart rate, increased SV-BA distance), hepatotoxicity (reduced liver area index), and neurotoxicity (impaired behavioral ability, and dopamine neuron development deficits), but there was no renal toxicity observed in zebrafish. Additionally, MALDI-MSI analysis showed that BO exposure significantly altered the levels of metabolites, including phospholipids, fatty acids, choline, and amino acids. The contents of PC-34:1, PC-34:2, PI-36:4, PE-36:1, LysoPE-22:5, LysoPC-18:1, FA-18:2, phenylalanine, lysine and glutathione were significantly increased, while the contents of PC-38:6 and PC-40:6 were significantly decreased. Notably, BO elicited a significant alteration in the mRNA expression levels of genes associated with phospholipid metabolism, fatty acid metabolism, choline metabolism, and amino acid metabolism (such as elovl5 , chpt1 , chka , setd7 , hgd ). This study revealed that BO exerted toxicity on multiple organs and demonstrated that BO causes metabolic dysregulation in zebrafish. These findings provide a novel insight into the toxicity of BO.
研究领域:
毒理学
·
环境科学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Yilong Zou,Yu Zhou,Xianglian Wang,Guiqing Gao,Gang Chen,Zhanmeng Liu,Mi Deng,Xueping Huang,Yongming Wu
Concerns regarding the possible health and environmental hazards of sodium p -perfluorous nonenoxybenzene sulfonate (OBS), a new substitute for perfluorooctane sulfonate, have been raised by its detection in a range of environmental matrices, animals, and people. Despite its widespread presence, research on the toxic effects and toxicokinetics of OBS remains limited. Therefore, in this study, we aimed to provide the first detailed investigation into how tissue distribution, bioaccumulation, and OBS exposure cause oxidative damage in adult zebrafish. Zebrafish were exposed to OBS at doses of 0, 10, and 100 μg/L for 28 days, following a 28-day depuration phase. Our findings indicate that OBS accumulated rapidly in zebrafish, with log kinetic bioconcentration factor (logBCF k ) values ranging from 2.48 to 2.70 and slow elimination rates ( k e = 0.0490–0.0643 d −1 ). The blood, liver, and muscle were responsible for over 50 % of the total body burden of OBS, with tissue concentrations decreasing in the following order: blood > liver > intestine > gills > gonad > brain > muscle, suggesting a tissue-specific accumulation pattern. Molecular docking analysis showed that OBS exhibited binding energies of −2.71 and −3.82 kcal/mol with organic anion transporter 1 and human serum albumin, respectively. Additionally, chronic exposure to OBS resulted in significant liver damage in zebrafish, including ballooning, cytoplasmic vacuolisation, and oxidative stress. This study provides valuable experimental data on the toxicokinetics and in vivo toxicology of OBS in zebrafish, offering important insights for environmental risk assessments.
研究领域:
环境科学
·
毒理学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 52
·33870-33875
·
IF 4.036
Sheng-Lin Pei,Jin Zhang,Wanyun Ge,Chao Liu,Ruilong Sheng,Lintao Zeng,Ling-Hui Pan
Biogenic amines (BAs) are a family of nitrogen-bearing natural organic molecules with at least one primary amine, which play an important role in living organisms. Elevated concentration of BAs may cause neuron disorder, Parkinson's disease and many other diseases. Therefore, it is essential to monitor BAs in living organisms. Herein, we reported a resorufin-based fluorescence probe for sensing of various BAs. Upon nucleophilic substitution reaction with BAs, the probe released resorufin, affording to strong fluorescence emission at 592 nm with rapid response (<8 min), good selectivity and a low detection limit (LOD = 0.47 μM). The probe has low cytotoxicity and good membrane permeability, and has been successfully used to visualize BAs in living cells and zebrafish with good performance.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
eLife
·
2024
·
IF 6.4
Xiaoning Wang,Jinxiang Zhao,Jiehuan Xu,Bowen Li,Xia Liu,Gangcai Xie,Xuchu Duan,Dong Liu
Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these noncaloric monosaccharides on blood vessel function remain inadequately understood. We have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling the hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, we observed that glucose and noncaloric monosaccharides could induce excessive formation of blood vessels, especially intersegmental vessels (ISVs). The excessively branched vessels were observed to be formed by ectopic activation of quiescent endothelial cells (ECs) into tip cells. Single-cell transcriptomic sequencing analysis of the ECs in the embryos exposed to high glucose revealed an augmented ratio of capillary ECs, proliferating ECs, and a series of upregulated proangiogenic genes. Further analysis and experiments validated that reduced foxo1a mediated the excessive angiogenesis induced by monosaccharides via upregulating the expression of marcksl1a. This study has provided new evidence showing the negative effects of noncaloric monosaccharides on the vascular system and the underlying mechanisms.
研究领域:
分子生物学
·
血管生物学
·
糖尿病研究
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Zhengliang Lu,Yanan Lu,Wenlong Fan,Chunhua Fan,Yanan Li
As the second most abundant transition metal after iron in biological systems, Zn 2+ takes part in various fundamental life processes such as cellular metabolism and apoptosis, neurotransmission. Thus, the development of analytical methods for fast detection of Zn 2+ in biology and medicine has been attracting much attention but still remains a huge challenge. In this report, we develop a novel Zn 2+ -specific light-up fluorescent probe based on intramolecular charge transfer combined with chelation enhanced fluorescence induced by structural transformation. Addition of Zn 2+ in vitro can induce a remarkable color change from colorless to green and a strong fluorescence enhancement with a red shift of 43 nm. Moreover, the probe shows an extremely low detection limit of 13 nM and ultra-fast response time of less than 1 s. The Zn 2+ sensing mechanism was fully supported by TDDFT calculations as well as HRMS and 1 H NMR titrations . The recognition of Zn 2+ in living Hela cells as well as the MTT assay demonstrate that the probe can rapidly light-up detect Zn 2+ in vivo with low cytotoxicity and good cell-permeability. Furthermore, the probe can also be successfully applied to bioimaging Zn 2+ in living zebrafish.
研究领域:
生物化学
·
医学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Meichen Liu,Yingxia Guo,Ziyi Niu,Yufeng Guo,Shuang Feng,Xiaoyan Zhang,Jiansong You,Lei Yin,Hongyu Xue,Meiyun Shi
A sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry cubed (UPLC-MS 3 ) assay was established for detection of fipronil in zebrafish tissue samples. Following protein precipitation pretreatment, the concentrations of fipronil in various zebrafish tissue samples were quantified by the developed UPLC-MS 3 assay. Fipronil was monitored by MS 3 transition ( m/z 435.0 → 398.9 → 330.0) with a ± 1.0 Da isolation window for secondary product ions. Chromatographic separation was achieved with 0.1 % formic acid-water ( v /v) as the aqueous phase and acetonitrile as the organic phase. Gradient elution with C 18 column was employed for separation of fipronil and probenecid (internal standard, IS). Method validation demonstrated excellent linearity over the range of 0.1–10 ng/mL (r 2 > 0.995), with the accuracies ranging from −10.33 % to 3.17 % and precisions between 3.25 % and 11.34 %. Consistent extraction recoveries (89.92–113.49 %) and acceptable matrix effects (92.54–110.27 %) were observed across all tested matrices. The implementation of MS 3 scanning significantly enhanced specificity by eliminating endogenous interference compared to conventional MRM detection. The successful application of this method in zebrafish tissue distribution studies confirmed its reliability for environmental toxicology research. This MS 3 -based approach provides a robust analytical platform for investigating biodistribution of fipronil in aquatic models.
研究领域:
分析化学
·
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）