JOURNAL OF MEMBRANE SCIENCE
·
2017
·
IF 6.035
Kuibo Zhang,Shoutao Gong,Baolin Zhao,Yanxiang Liu,Naeem Akhtar Qaisrani,Lingdong Li,Fengxiang Zhang,Gaohong He
Inadequate hydroxide conductivity of anion exchange membrane (AEM) is one of the obstacles restricting practical application of anion exchange membrane fuel cell. In this work, we report the design, fabrication and properties of AEMs based on bent-twisted non-coplanar block copolymer . The bent-twisted polymer backbone creates high free volume size so that hydroxide ion transport is facilitated. The free-volume effect can also facilitate microphase separation due to enhanced chain segment motion. With the free-volume holes working synergistically with microphase-separation enabled ion channels, the membrane showed a high conductivity (35 mS cm −1 at room temperature) at a relatively low ion exchange capacity (IEC, 1.25 meq g −1 ); it also exhibited a good dimension stability, the swelling ratio being 4.7% at room temperature and kept virtually unchanged with temperature because of relatively low IEC. A H 2 /O 2 fuel cell employing the fabricated AEM yielded a high power density (262 mW cm −2 at 50 °C). Our work opens up a new route to fabrication of highly conductive AEM with relatively low IEC.
研究领域:
材料科学
·
燃料电池工程
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Ming Li,Wenying Lu,Yuhao Mao,Xuchun Qiu,Daolin Du
It is desired and urgently needed to improve the sensitivity of immunochromatography assay (ICA) for hazardous chemicals. In this work, an enhanced ICA strip was established and evaluated for simultaneous, semi-quantitative and quantitative detection of ochratoxin A (OTA) and aflatoxin B 1 (AFB 1 ). The signal strategy based on gold growth on the surface of the E. coli K12 carrier was recommended, which was successfully self-assembled on the enhanced ICA with a double test line pattern. When used as a novel carrier, the E. coli K12 could provide a larger surface area, better biocompatibility and high loading capacity, which was of great help to improve the performance of the ICA. By the naked eye, the semi-quantitative limit of detection (semi-Q-LOD) reached 0.03 ng/mL for both OTA and AFB 1 (17-fold and 33-fold lower than the conventional ICA strip). By the digitized strip reader, the quantitative LODs (Q-LODs) were all identified as 0.01 ng/mL for two mycotoxins (10-fold improvement), with detection ranges of 0.01–0.5 ng/mL for OTA and 0.01–0.2 ng/mL for AFB 1 . Furthermore, the high reliability and applicability of the ICA were confirmed by its good correlation with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The advantages of the improved sensitivity, high efficiency and cost savings had been reflected for the ICA. This study could provide an important reference method for the sensitive, simultaneous, rapid and on-site monitoring of multicomponent contaminants.
研究领域:
食品安全检测
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Chen Zong,Fan Jiang,Xiaoyu Wang,Ping Li,Linru Xu,Hua Yang
A novel chemiluminescence (CL) immunosensor array in combination with a dual-signal amplification strategy was developed for rapid and ultrasensitive detection of multiple mycotoxins in herbal medicine. The multi-component immunosensor array was constructed by immobilizing different bovine serum albumin combined mycotoxins on the corresponding sites of the aldehyde-modified glass slide. After competitive immunoreactions, CL triggered by signal tags captured on all the sensing sites can be collected by a charge-coupled device simultaneously for joint detection of several mycotoxins. By assembling a high ratio of horseradish peroxidase (HRP) and IgG on the surface of gold nanoparticles (AuNPs), a biofunctionalized complex named [email protected] was prepared and served as the primary signal tag to amplify the CL signals. Then, by introducing tyramine signal amplification (TSA) technique, a new round of HRP could deposit around the [email protected] , which brought the secondary CL amplification. As a proof of concept , the CL imaging sensor array was applied to the trace detection of citrinin , aflatoxin B 1 and ochratoxins A in red yeast rice samples. Under optimal conditions, it exhibited wide linear ranges over 4 orders of magnitude and much lower limits of detection than previous works. Owing to the excellent sensitivity (50–57-fold signal amplification and detection limits down to sub-pM level), acceptable throughput (20 tests h −1 ), small amounts of reagents (3.5 μL for each test), simple sample pretreatment (no necessary of separation for 3 mycotoxins), high selectivity , acceptable stability and accuracy, the proposed sensing platform showed broad prospects in the joint monitoring of low-abundant mycotoxins and safety evaluation of herbal medicines.
研究领域:
分析化学
·
生物技术
·
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Jiawei Zhang,Jie Zhao,Xinxin Shi,Zhiwei Sun,Jinmao You
Mycotoxins are frequently found in food samples and have become a major concern in the maintenance of food safety and human health. In this study, we developed an isotope-coded derivatization (ICD) method for the quantification of six mycotoxins in various complex matrices by using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). The targeted mycotoxins were ochratoxin A, ochratoxin B, fumonisin B1, fumonisin B2, zearalenone , and zearalanone . The mycotoxins in the actual samples and standards were derivatized with d 0 -dansyl chloride and its heavy form, d 4 -dansyl chloride. A mixture of light-isotope- and heavy-isotope-labeled derivatives was subjected to UHPLC-MS/MS, and isotopic internal calibration quantification of all targeted mycotoxins was achieved. The retention time difference between light-isotope- and heavy-isotope-labeled derivatives was less than 0.02 min, and the analysis could be completed within 7.5 min. The developed method was validated in terms of its sensitivity, precision, and accuracy. The obtained limits of detection were in range of 31.03–38.15 ng/L, and the accuracy was 97.13–104.1% with a precision of less than 3.42%. This method was also successfully applied to analyze mycotoxins in three selected cereal samples (corn, wheat, and sorghum), achieving recoveries of 71.3–92.6%. The ICD strategy provides an alternative assay to isotopic internal standards, thereby helping to effectively minimize matrix effects during mass spectrometry analysis . The proposed ICD-based UHPLC-MS/MS method has high potential for the determination of amino- and phenol-containing mycotoxins in various food samples.
研究领域:
食品安全
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
Bioanalysis
·
2025
·
IF 1.8
Kaiwei Luo,Danrui Liu,Shengshuo Zhang,Xin Zhang,Qunan Cheng,Jie Lin,Yonghua Yu
Aims This study aimed to establish a robust LC-MS/MS method for quantifying trazodone in human plasma and investigate its pharmacokinetic profile in healthy Chinese subjects under fasting and fed conditions.Materials & methods A validated LC-MS/MS method using protein precipitation was applied to analyze trazodone in 68 healthy subjects (34 fasting/34 fed), with full method validation performed.Results This method exhibited high selectivity, precision, and accuracy. The results showed good linearity in the range of 5–3000 ng/mL and matrix effect results showed no matrix interference. The pharmacokinetic data revealed notable differences between the fasting and postprandial states.Conclusions The validated LC-MS/MS method proved reliable for trazodone quantification. The observed food effects on absorption suggest clinical dosing should consider administration conditions to ensure optimal therapeutic outcomes.
研究领域:
分析化学
·
药理学
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yifeng Lan,Yingqi Ma,Yingwen Xu,Yuan Zhang,Rujia Deng,Lulu Liu,Boya Hou,Haiyan Cui,Keming Yun,Zhiwen Wei,Yanli Wei
Effective truncation and mutation can obtain highly active nucleic acid aptamers for the target, especially avoiding non-specific binding, uncertainty to the tertiary structure and low binding affinity of the lengthy aptamer. In this work, a new aptamer, T-AF28 (28 nt) with better affinity to AFB1 was obtained by truncating 22 bases and mutating 3 bases of the reported AFB1 aptamer (50 nt), and the thermodynamic information, structural changes and binding domains of aptamer T-AF28 in the presence of AFB1 or AFM1 were further investigated by isothermal titration calorimetry, circular dichroism, and molecular dynamics. On this basis, Using optimized aptamers as recognition elements, a novel label-free and enzyme-free fluorescent aptasensor for AFB1 and AFM1 was developed by synergistic amplification effect of hybridization chain reaction (HCR) coupled molybdenum disulfide nanosheets and copper nanoparticles in situ synthesized (CuNPs) on an extra-long double-stranded template generated by HCR as the fluorescent probe. Under the optimal experimental conditions, sensitive and selective fluorescent detection of AFB1 and AFM1 was achieved with wide linear detection ranges of 0.030 ng/mL-46.8 ng/mL and 0.030 ng/mL-32.8 ng/mL, respectively. And this aptasensor can be applied to accurate detection of AFB1 and AFM1 in grains, edible oil, tea, and milk with the recovery in the range of 85.20 %-117.0 %. This work not only provides a theoretical basis for the design of AFB1 and AFM1 aptasensors and expands the application of AFB1 aptasensors in the field of food safety, but also breaks a new path for the development of HCR-MoS 2 Ns CuNPs aptasensors.
研究领域:
食品安全
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Microbiology Spectrum
·
2023
·Vol. 11
·期 4
·
IF 3.7
Liuke Liang,Wei Zhang,Jing Hao,Yanyu Wang,Shan Wei,Shuaibing Zhang,Yuansen Hu,Yangyong Lv
A variety of essential oils and edible compounds have been widely recognized for their antifungal activity in recent years. In this study, we explored the antifungal activity of estragole from Pimenta racemosa against Aspergillus flavus and investigated the underlying mechanism of action. The results showed that estragole had significant antifungal activity against A. flavus, with a minimum inhibitory concentration of 0.5 μL/mL against spore germination. Additionally, estragole inhibited the biosynthesis of aflatoxin in a dose-dependent manner, and aflatoxin biosynthesis was significantly inhibited at 0.125 μL/mL. Pathogenicity assays showed that estragole had potential antifungal activity against A. flavus in peanut and corn grains by inhibiting conidia and aflatoxin production. Transcriptomic analysis showed that the differentially expressed genes (DEGs) were mainly related to oxidative stress, energy metabolism, and secondary metabolite synthesis following estragole treatment. Importantly, we experimentally verified reactive oxidative species accumulation following downregulation of antioxidant enzymes, including catalase, superoxide dismutase, and peroxidase. These results suggest that estragole inhibits the growth and aflatoxin biosynthesis of A. flavus by modulating intracellular redox homeostasis. These findings expand our knowledge on the antifungal activity and molecular mechanisms of estragole, and provide a basis for estragole as a potential agent against A. flavus contamination.IMPORTANCE Aspergillus flavus contaminates crops and produces aflatoxins, carcinogenic secondary metabolites which pose a serious threat to agricultural production and animal and human health. Currently, control of A. flavus growth and mycotoxin contamination mainly relies on antimicrobial chemicals, agents with side effects such as toxic residues and the emergence of resistance. With their safety, environmental friendliness, and high efficiency, essential oils and edible compounds have become promising antifungal agents to control growth and mycotoxin biosynthesis in hazardous filamentous fungi. In this study, we explored the antifungal activity of estragole from Pimenta racemosa against A. flavus and investigated its underlying mechanism. The results demonstrated that estragole inhibits the growth and aflatoxin biosynthesis of A. flavus by modulating intracellular redox homeostasis.
研究领域:
真菌学
·
食品科学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 2
·266
·
IF 4.7
Zhuzhu Qiu,Fenghua Wu,Hao Hu,Jian Guo,Changling Wu,Peng Wang,Jiangang Ling,Yan Cui,Jing Ye,Guanyu Fang,Xingquan Liu
Different storage conditions can influence microbial community structure and metabolic functions, affecting rice grains’ quality. However, the microbiological mechanisms by which different storage conditions affect the quality of rice grains are not yet well understood. This study monitored the quality (the content of starch, protein, etc.) and microbial community structure of rice grains stored under different storage conditions with nitrogen gas atmosphere (RA: normal temperature, horizontal ventilation, RB: normal temperature, vertical ventilation, RC: quasi-low temperature, horizontal ventilation). The results revealed that the rice grains stored under condition RB exhibited significantly lower quality compared to condition RA and RC. In addition, under this condition, the highest relative abundance ofAspergillus(16.0%) andPenicillium(0.4%) and the highest levels of aflatoxin A (3.77 ± 0.07 μg/kg) and ochratoxin B1 (3.19 ± 0.05 μg/kg) were detected, which suggested a higher risk of fungal toxin contamination. Finally, co-occurrence network analysis was performed, and the results revealed that butyl 1,2-benzenedicarboxylate was negatively correlated (p< 0.05) withMoesziomycesandAlternaria. These findings will contribute to the knowledge base of rice storage management and guide the development of effective control measures against undesirable microbial activities.Keywords:rice grains storage;mycotoxins;microbial communities
研究领域:
食品科学
·
微生物学
·
农业工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROTECTION
·
2025
·
IF 2.8
Yaoyao Su,Jun Huang,Xiaoman Chen,Gang Yang,Kewei Chen,Muying Du,Zsolt Zalán,Ferenc Hegyi,Sameh Awad,Jianquan Kan
The contamination of spices with aflatoxin (AF) raises concerns regarding its health risks. In this study, 51 samples of dried red chili peppers (including 10 varieties collected from three regions) were used to investigate the AF contamination of dried red chili peppers sold in China, and the toxigenic characteristics of Aspergillus flavus strains isolated from chili peppers. AFs were identified using immunoaffinity column purification, derivatization, and high-performance liquid chromatography with fluorescence detection. The AF content in various types of dried red chili peppers sold in the Chinese market was generally low (≤ 7.19 µg/kg), below the limit of the relevant standard (10 µg/kg). The frequency of suspected A. flavus strains isolated from chili peppers was high (24/51), with a high toxigenic strain ratio of 29.17%. Further investigation revealed that 25–30 ℃ was the optimal temperature for toxin production by these toxigenic strains. This indicated that dried chilled peppers have a significant risk of AF contamination. Therefore, the findings of this study suggest that long-term storage of dried chili peppers should be conducted at temperatures below 25 ℃ or above 37 ℃ to mitigate the problem of AF contamination exceeding the levels allowed by the standards, providing new insights for the prevention and control of AF contamination of dried red chili peppers.
研究领域:
食品科学
·
微生物学
·
毒理学
·
农业科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2024
·
IF 1.2
Yuzheng CAI,Ge GUO,Yankun FU,Xianqing HUANG,Tianlin WANG,Tiange LI
Simultaneous and rapid detection of various mycotoxins in food holds significant practical importance in the field of food processing and safety. In this study, a fluorescent aptasensor based on functionalized graphene oxide (FGO) is developed for simultaneous detection of aflatoxin B 1 (AFB 1 ) and aflatoxin M 1 (AFM 1 ). The two aptamers specific to AFB 1 and AFM 1 are labeled with Cy3 and Cy5 respectively. Both the aptamers can be adsorbed onto the surface of FGO through π - π stacking, resulting in fluorescence resonance energy transfer (FRET) between the fluorophore and FGO. The absence of target leads to quenching of fluorescence while presence of either aflatoxin causes interaction between corresponding aptamer and target, leading to release from FGO surface thereby turning on fluorescence signal. The limit of detection (LOD) for AFB 1 is determined as 8.7 pg/mL whereas for AFM 1 it is found to be 20.1 pg/mL, demonstrating fast and sensitive detection capability using this approach. Furthermore, the aptasensor exhibits good specificity and selectivity even under influence from other common interfering toxins. With its simplicity in operation and portability features, this sensor has potential applications for establishing sensitive and portable on-site detection methods for various mycotoxins.
研究领域:
食品安全
·
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-10
·
IF 16.6
Du Wei,Gao Feng,Cui Peng,Yu Zhiwu,Tong Wei,Wang Jihao,Ren Zhuang,Song Chuang,Xu Jiaying,Ma Haifeng,Dang Liyun,Zhang Di,Lu Qingyou,Jiang Jun,Wang Junfeng,Pi Li,Sheng Zhigao,Lu Qingyi
The reversible transformation of a nanohelix is one of the most exquisite and important phenomena in nature. However, nanomaterials usually fail to twist into helical crystals. Considering the irreversibility of the previously studied twisting forces, the reverse process (untwisting) is more difficult to achieve, let alone the retwisting of the untwisted crystalline nanohelices. Herein, we report a new reciprocal effect between molecular geometry and crystal structure which triggers a twisting-untwisting-retwisting cycle for tri-cobalt salicylate hydroxide hexahydrate. The twisting force stems from competition between the condensation reaction and stacking process, different from the previously reported twisting mechanisms. The resulting distinct nanohelices give rise to unusual structure elasticity, as reflected in the reversible change of crystal lattice parameters and the mutual transformation between the nanowires and nanohelices. This study proposes a fresh concept for designing reversible processes and brings a new perspective in crystallography.
研究领域:
纳米材料
·
晶体学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Qiuxiang Tang,Yi Zhang,Jun Huang,Rongqing Zhou
Tri-colored Jiangqu , including white (WQ), yellow (YQ), and black (BQ)-color Daqu , significantly influence fresh Baijiu yield and quality. The differences in attributes of these Jiangqu types, sourced from two renowned Baijiu enterprises, were investigated using multi-omics approaches. Color intensity negatively correlated with ammonia nitrogen content, but positively with pyroglutamic acid content. 182 volatiles and 291 non-volatiles were identified, with each types exhibiting unique metabolites. Esters, pyrazines, ketones and phenols were predominant in WQ, YQ, and BQ, respectively. The content of peptides, amines, and amino acids with derivatives also showcased the differences of microbiota and metabolic pathways among tri-colored Jiangqu . BQ's characteristic components of L-Tyrosine and acetyl tributyl citrate were closely associated with melanin formation. In YQ, Scopulibacillus and Rhizopus correlated positively with tetramethylpyrazine. Moreover, the bacterial community significantly influenced the metabolic profiles, and synergistic interactions between fungal and bacterial communities were crucial in determining metabolite abundance.
研究领域:
微生物学
·
食品科学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）