TALANTA
·
2024
·
IF 5.6
Yuan Gao,Bingfeng Bai,Zenghui Mao,Xiao Yang,Yi Shi,Bo Zhang,Ke Quan,Zhihe Qing
The daily consumption of foods abundant in Glutathione (GSH) can be supplemented to maintain the homeostasis of GSH in human health and alleviate pathologies resulting from abnormal GSH levels. The fluorescence-based visual determination of GSH has gradually attracted the attention of researchers due to its robust performance and versatile implementation. However, the current GSH visual strategy primarily relies on variations in fluorescence intensity at a single emission wavelength, which poses challenges for naked-eye and portable readout, as well as distorted signals caused by complex matrix effects in real samples. Herein, a ratiometric fluorescence sensor based on carbon dots (CDs) combined with an all-in-one 3D-printed smartphone-based device was successfully developed for low-cost, visual and rapid detection of GSH without the need for an external excitation light source. The ratiometric fluorescent materials were synthesized by conjugating blue carbon dots (B-CDs) with yellow carbon dots (Y-CDs) through the utilization of selected Cu 2+ ions. The resulting mechanism demonstrated that the coordination interaction between Cu 2+ and residual aromatic amino groups in Y-CDs (Y-CDs-Cu 2+ ) contributed to a newly emitted peak at 580 nm, thereby inducing fluorescence resonance energy transfer from B-CDs to Y-CDs-Cu 2+ . A linear correlation was found between GSH concentrations and R/B values in the range of 10–100 μM, with a limit of detection observed at 4.8 μM. By utilizing this portable device in combination with RGB analysis, the quantitative detection of GSH can be achieved even in complex food matrices such as tomatoes and grapes. The universality of this all-in-one device was further validated by pre-spraying CDs onto a paper strip for visual measurement of GSH. This work offers a portable, visual, and accessible approach to evaluating food safety and nutrition, thereby demonstrating significant economic value and holding profound implications for human health.
研究领域:
分析化学
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生物医学工程
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食品安全
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-9
·
IF 5.3
Guo Dongying,Yang Hailiang,Huang Yuankui,Hu Yaoping
A novel ratiometric fluorescence sensor based on a hydrophilic/hydrophobic carbon dot (CD) pair is presented for sensitive detection of water in tetrahydrofuran (THF). Hydrophilic blue-emissive CDs (B-CDs) and hydrophobic orange-emissive CDs (O-CDs) are synthesized via a one-pot hydrothermal method, exhibiting distinct structural and optical properties. While B-CDs maintain stable fluorescence in both THF and water, O-CDs undergo aggregation-induced fluorescence quenching upon incorporation of water in THF. Leveraging this contrast, the B-CDs/O-CDs dual-emission system enables ratiometric quantification of water content in a wide range of 0–70% (v/v) with a low detection limit of 0.016%. The intensity ratio ( I orange / I blue ) and emission peak shift (Δ λ orange ) provide dual-mode detection, ensuring high accuracy. Additionally, a portable polyvinyl alcohol (PVA) film embedded with the CD pair allows smartphone-assisted on-site determination of water content, achieving recoveries of 91.0–114.0% in commercial THF samples. This work offers a cost-effective and instrument-free approach for water determination, with potential applications in solvent quality control and industrial process optimization. Graphical
研究领域:
分析化学
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材料科学
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环境监测
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DOI
PubMed
高影响因子
CARBON
·
2014
·
IF 6.16
Yu Zou,Fanyong Yan,Linfeng Dai,Yunmei Luo,Yang Fu,Ning Yang,Jingyun Wun,Li Chen
Highly blue fluorescent carbon nanodots (CNDs) were synthesized by the hydrothermal method from citric acid and diethylenetriamine with the quantum yield of about ∼86%. Quercetin (QCT) and its fluorescent metal-ion complex (QCT-Al 3+ ) can efficiently coordinate on the surface of CNDs, the emission of CNDs at 429 nm completely overlapped with the absorption peak of QCT-Al 3+ at 428 nm. Therefore, a ratiometric fluorescence sensor for Al 3+ was developed based on fluorescence resonance energy transfer (FRET) with CNDs as donor and QCT-Al 3+ as acceptor. A linear calibration of F 429 / F 481 versus aluminum ion concentration was obtained within 1–20 and 20–60 μM, along with a detection limit of 558 nM. Moreover, the ESIPT–FRET ratiometric fluorescence system based on the CNDs/QCT-Al 3+ was applied for fluorescent test papers with satisfying resolution.
研究领域:
分析化学
·
材料科学
·
生物传感
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Huiping Wang,Huaizhong Xing,Wei Liu,Yumin Hao,Li Zhang,Zhenhua Yang,Qin Hu,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
Ratiometric fluorometry is an effective method for the quick and sensitive detection of the target objects by reducing the background interference. Carbon dots (Cdots), especially metal-doped carbon dots (M-Cdots), have been extensively developed as ratiometric fluorescence sensors and widely used in materials, chemistry , biomedicine, etc. Herein, we reported a simple and economical one-step hydrothermal method for the synthesis of gadolinium-doped carbon dots (Gd-Cdots) by treating Gd(NO 3 ) 3 .6H 2 O and 3-aminothiophenol. The Gd-Cdots afford good fluorescence properties, high T 1 relaxivity, and excellent biocompatibility, making them an intrinsical nano-sensor and dual-mode imaging reagent. Based on the unique chelation between Gd and morin, a ratiometric fluorescence nano-sensor for the detection of morin was constructed with the linear range of 0.04–80.0 µM and the detection limit of 25.0 nM. Meanwhile, the color of the Gd-Cdots varied from colorless to deep-yellow, so a colorimetric nano-sensor was developed for morin detection with the linear ranges and the detection limits of 0.04–15.0 µM and 10.5 nM and 15.0–93.0 µM and 21.2 nM, respectively. Compared with the reported fluorescent or colorimetric sensors for the detection of morin, the constructed ratiometric fluorometry and colorimetry nano-sensor based on the Gd-Cdots owns higher sensitivity, lower detection limit, wider linear range, and less background interference. More importantly, the nano-sensor based on the Gd-Cdots was successfully applied to detect morin in human urine and living cells. Overall, the present study shows that the Gd-Cdots possess distinct optical and toxicological properties and are outstanding nanomaterials for the construction of nano-sensors and cellular imaging.
研究领域:
材料科学
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分析化学
·
生物医学工程
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DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·
IF 1.193
Kun WANG,Er-Fei DONG,Min FANG,Ting CHEN,Wei-Ju ZHU,Cun LI
A new ratio fluorescence sensor based on mercaptamine capped cadmium telluride quantum dots (Mea-CdTe) and benzocoumarin-3-carboxylic acid (BTA) were constructed. The ratio fluorescence sensor (BTA@Mea-CdTe) was synthesized by amidation reaction between the amino groups on the surface of Mea-CdTe quantum dots and the carboxyl groups of BTA. It is found that Hg 2+ ions react with the amino groups on the surface of the quantum dots in the BTA@Mea-CdTe sensor to quench the fluorescence, but it has no effect on the fluorescence of grafted BTA. The sensor has good selectivity and high sensitivity for Hg 2+ ions detection. The detection linear range is 0.4–1.2 µM, and the detection limit is 0.03 µM. This ratio fluorescence sensor can be used for Hg 2+ visual recognition in water sample detection with obvious color change.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-13
·
IF 5.3
Li Chao,Ma Yangmin,Fan Cheng,He Chong,Ma Siyue
A dual-emission ratiometric fluorescence sensor (CDs@CdTe@MIP) with a self-calibration function was successfully constructed for AMO detection. In the CDs@CdTe@MIP system, non-imprinted polymer-coated CDs and molecule-imprinted polymer-coated CdTe quantum dots were used as the reference signal and response elements, respectively. The added AMO quenched the fluorescence of the CdTe quantum dots, whereas the fluorescence intensity of the CDs remained almost unchanged. The AMO concentration was monitored using the fluorescence intensity ratio (log(I 647 /I 465 ) 0 /(I 647 /I 465 )) to reduce interference from the testing environment. The sensor with a low detection limit of 0.15 μg/L enabled detection of the AMO concentration within 6 min. The ratiometric fluorescence sensor was used to detect AMO in spiked pork samples; it exhibited a high recovery efficiency and relative standard deviation (RSD) of 97.94–103.70% and 3.77–4.37%, respectively. The proposed highly sensitive and selective platform opens avenues for sensitive, reliable, and rapid determination of pharmaceuticals in the environment and food safety monitoring using ratiometric sensors. Graphical Abstract
研究领域:
分析化学
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食品安全
·
环境科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Dong Zhang,Ping Yan,Hong Yu,Jing Sun,Shuyun Zhu,Xian-En Zhao
Development of accurate, convenient and portable methods for monitoring 4-aminophenol (4-AP) is extremely important because of its strong toxicity. Here, a ratiometric fluorescence sensor based on Ag + -enhanced luminescence of Tb-DNA complexes has been presented for the detection of 4-AP. The luminescence of Tb-DNA complexes is enhanced about 30 times by Ag + , which can trigger energy transfer from DNA to Tb 3+ more efficiently. In the presence of 4-AP, Ag + can be reduced into Ag 0 owing to its strong reducibility, inducing the decrease of Tb-DNA complexes at 545 nm remarkably. Besides, 4-AP exhibits intrinsic fluorescence at 375 nm under the same conditions. Therefore, ratiometric detection of 4-AP can be achieved using F 375 /F 545 as readout with a detection limit of 0.4 μM. Real water samples have been tsted to evaluate this method in practical application and satisfactory results have been obtained. Furthermore, the addition of 4-AP into Tb-DNA-Ag + system induces a visible color variation from green to bluish violet, enabling visual detection of 4-AP under the assistance of a smartphone.
研究领域:
分析化学
·
环境科学
·
生物传感
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zhe Li,Xiaohui Wang,Xiuli Fu,Jinhua Liu,Yuanan Liu,Hongxin Zhang
The pH and O 2 are the two important parameters to evaluate environmental conditions, healthcare, and industrial processes. Herein, we developed a ratiometric fluorescent capillary-based dual-functional sensor for simultaneous monitoring of pH and O 2 fluctuations. The fluorescent sensor consists of a pH sensing unit and an O 2 sensing unit on both ends of the capillary inner wall. The ratiometric fluorescent pH sensing unit includes pH-sensitive probe HPTS and the reference dye Ce6, while the O 2 sensing unit is composed of O 2 -sensitive probe RuDP and the reference dye C6. Under single-wavelength excitation at 430 nm, the ratiometric fluorescent dual-functional sensor exhibits highly sensitive, selective and reproducible responses to both pH and O 2 . As for practical aqueous samples, the results about pH and O 2 levels obtained by our sensor are considerable consistent with commercial devices. Meanwhile, the dynamic changes of pH and O 2 levels during glucose catalysis by glucose oxidase can be detected in real-time and accurately. The dual-functional ratiometric fluorescent capillary transducer provides a significant potential platform for environmental monitoring and medical diagnosis.
研究领域:
生物医学工程
·
环境科学
·
化学传感
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Linli Hou,Yonghai Song,Yingjie Xiao,Rong Wu,Li Wang
Lanthanide-based fluorescence sensor in the detection of major Anthrax biomarker dipicolinic acid (DPA) is attracting wide attention. In this work, we proposed a new strategy for ratiometric fluorescence detection of DPA based on microporous Ln/melamine-terephthaladehyde Schiff base networks (Ln/MTSNW) complex for the first time. The microporous MTSNW was prepared by amine-aldehyde condensation between melamine and terephthaladehyde and presented lamellar and octahedral structure. Lanthanide ions, Eu 3+ or Tb 3+ were coordinated with N atoms of MTSNW to form Ln/MTSNW complex. The microporous Ln/MTSNW complex not only provided large surface area to improve the sensitivity of DPA detection, but also constructed ratiometric fluorescence sensors to eliminate environmental effects and instrument fluctuation. DPA was a highly efficient antenna molecule for Eu 3+ and Tb 3+ and transferred the energy to Eu 3+ or Tb 3+ to sensitize their fluorescence. The Ln/MTSNW complex were uniformly and stably dispersed in aqueous solution for DPA detection with a linear range from 15 nM to 7 μM and low detection limit of 5.2 nM for Eu/MTSNW and a linear range from 4 nM to 2.5 μM and low detection limit of 1.4 nM for Tb/MTSNW. Due to the simple preparation of Ln/MTSNW complex and low technical requirement, the ratiometric fluorescence DPA sensor based on Ln/MTSNW complex might show great potential in practical applications.
研究领域:
生物化学
·
分析化学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Ying Cao,Jiaojiao Liu,Lina Zou,Baoxian Ye,Gaiping Li
In this work, a facile ratiometric fluorescence sensor for GSH measurement was designed based on MnO 2 nanosheet (NS), carbon dots (CDs), as well as a simple substrate o-phenylenediamine (OPD). Herein, MnO 2 NS played triple essential roles in the sensing system. First, it could be reduced by GSH through a special reaction, and therefore served as GSH recognizer. Second, it played as a fluorescence nanoquencher to strongly quench the fluorescence of CDs. Third, it could directly oxidize OPD to yield a luminescent product 2, 3-diaminophenazine (DAP) via the intrinsic oxidase-like activity. It revealed that MnO 2 NS could be reduced to Mn 2+ in the presence of GSH. Thus its oxidase-like activity and fluorescence quenching abilities were inhibited, which then restricted the generation of DAP and recovered the fluorescence of CDs. Based on this phenomenon, a novel ratiometric fluorescence sensor for GSH determination was fabricated by measuring the ratio of fluorescent intensity of DAP to that of CDs. Besides, the constructed ratiometric fluorescent sensor, which could be facilely operated with single-wavelength excitation, exhibited high sensitivity and selectivity with a wider linear range and a lower detection limit.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Chao Li,Yangmin Ma,Cheng Fan,Yu An,Siyue Ma
The problem of excessive use and abuse of antibiotics in the environment and food biology is becoming increasingly prominent, and norfloxacin (NOR) is widely used as an inexpensive and broad-spectrum antibiotic drug. Therefore, rapid and effective detection of antibiotics and residues in biological samples has become a demand of the times. This article describes a ratiometric fluorescence sensor based on molecular imprinting technology for the rapid and visual detection of NOR. A linear relationship (R 2 = 0.9977) was established between the ratio of self-fluorescence of NOR to the reference fluorescence from rhodamine B and NOR concentration (0–400 μg/L), with a detection limit as low as 0.38 ng/mL, the detection time is as short as 8 min, and a high imprinting factor of 4.5. Visual detection of NOR was achieved through the change of fluorescence color from red to blue-purple. Satisfactory detection accuracy (RSD < 3.5 %) and recovery rate (90.03–102.10 %) were obtained through real sample spiking experiments and were highly consistent with HPLC-UV results. This ratiometric fluorescent sensor based on molecularly imprinting technology with high selectivity, sensitivity, rapidity, and visualization for detecting NOR in complex matrices has broad application prospects in the fields of rapid screening and on-site detection.
研究领域:
环境科学
·
分析化学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 5
·532-540
·
IF 3.532
Hua-Rui Nan,Yun-Hai Liu,Wen-Juan Gong,Hong-Bo Peng,You-Qun Wang,Zhi-Bin Zhang,Xiao-Hong Cao
In this work, a ratiometric fluorescence system was designed for the detection of trace UO22+ in water based on the inner filter effect (IFE) between gold nanoparticles (AuNPs) and gold nanoclusters (AuNCs). IFE-induced fluorescence quenching was achieved due to the enhanced complementary overlap between the absorption spectra of AuNPs and the emission spectrum of AuNCs after the addition of UO22+. Blue carbon dots (B-CDs) were added to serve as reference fluorophores to expand the color tonality and make human eye recognition easier. The ratiometric fluorescent sensor demonstrated a unique fluorescence color change from red to blue when different doses of UO22+ were added, with a detection limit of 8.4 nM. Furthermore, the ratiometric fluorescent sensor was effectively used for UO22+ determination in real-world water samples, with acceptable recoveries.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
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