Frontiers in Bioengineering and Biotechnology
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2025
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IF 4.8
Yaping Ma,Minchang Liu,Ruifang Gu,Rongya Zhang,Xiaomei Ji,Juan Zhang,Wu Wen,Zheng Peng
Introduction: Laccase exhibits significant applications in food additives, wastewater treatment, and biomass processing. Fungal laccase exhibits high activity, good stability, and excellent performance. However, scale-up production of high-yield laccase from fungi is challenging. This study aimed to identify crucial factors affecting enzyme production and analyze the enzymatic properties of laccase during fermentation.Methods: A laccase-producing white-rot fungus was used for fermentation process optimization in 200 L and 1200 L fermenters. The Plackett–Burman design revealed three significant influencing factors: temperature, aeration ratio, and agitation speed. The steepest ascent experiment was used to approximate the maximum response region, followed by the establishment of a regression model between experimental factors and laccase activity using the Box–Behnken response surface methodology and optimal fermentation condition selection.Results: The optimal conditions for laccase production by Ganoderma lucidum fermentation were 30°C temperature, 0.66 aeration ratio, and 100 rpm agitation speed, achieving a maximum laccase activity of 214,185.2 U/L. Dissolved oxygen (DO) was a crucial factor for high laccase yield, and its maintenance at a high level in the fermentation system significantly enhanced the enzyme activity. Fermentation batches with varying laccase production levels exhibited a trend of decreasing pH and a subsequent increase in the mid-to-late fermentation stages. With decreased pH, the DO level started declining; when DO stabilized, the pH started to rebound, coinciding with the peak laccase activity, indicating a signal of fermentation endpoint in industrial production.Discussion: This study provides valuable theoretical and data support for the industrial production of laccase by fungi through optimized fermentation processes.
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PEST MANAGEMENT SCIENCE
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2025
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IF 3.8
Jiulong Wang,Wenjing Yuan,Xinying Duan,Wuxiong Wang,Jie Song,Shangxing Chen,Hai Luo,Ji Zhang,Zongde Wang,Shengliang Liao,Hongyan Si
BACKGROUND Pear anthracnose, caused by Colletotrichum fructicola , is a highly destructive disease that inflicts substantial economic losses worldwide. However, current antifungal agents mainly target known target proteins, and long-term use leads to increased resistance of C. fructicola . Thus, developing multitargeted antifungal agents based on new targets is an effective way to solve the problem of drug resistance. RESULTS Twenty camphor derivatives containing thiosemicarbazide groups were synthesized and evaluated for antifungal activity against C. fructicola . Among them, compound 1a [median effective concentration (EC 50 ) = 3.76 mg L −1 ] exhibited the strongest antifungal activity, superior to kresoxim-methyl (EC 50 = 9.69 mg L −1 ). The in vivo antifungal test demonstrated that 1a had better protective and curative effects against pear anthracnose than kresoxim-methyl. The toxicity results showed that 1a exhibited low toxicity to LO2 and HEK293 cell lines. Mechanistically, 1a can destroy hyphae morphology and cell ultrastructure, increase cell membrane permeability, affect the normal expression pathway of soluble proteins and destroy the normal metabolic function of phospholipase C. Additionally, the laccase inhibitory activity results showed that 1a [Median inhibitory concentration (IC 50 ) = 7.14 mg L −1 ) had stronger laccase inhibitory activity than cysteine (IC 50 = 23.99 mg L −1 ). Furthermore, molecular docking studies showed that 1a interacted with laccase by forming hydrogen bonds with amino acid residues ASP444 and ARG442. CONCLUSION This study showed that compound 1a can serve as a promising multitarget fungicide candidate for controlling C. fructicola , with its targets at least including the cell membrane and laccase of the C. fructicola . © 2025 Society of Chemical Industry.
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Surfaces and Interfaces
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2025
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IF 6.3
Guolin Zheng,Wentao He,Haonan Cheng,Xinjun Bao,Anlin Zhang,Kun Wang,Bin He,Hengshu Zhou
To our knowledge, the protective coating on textiles has attracted tremendous interest since the outbreak of COVID-19, prompting significant advancements in protective textiles. Herein, the laccase-platinum nanozyme (Lac-Pt) was selected for the first time to catalyze the self-polymerization of epicatechin, in which the epicatechin polymer (PEC) was coated on cotton fabric by in-situ deposition, labeled as CF@PEC. The PEC coating on cotton fabric was confirmed through scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and energy-dispersive X-ray spectroscopy (EDX). Moreover, the self-polymerization mechanism of EC was demonstrated by UV-visible spectra, hydrogen proton nuclear magnetic resonance ( 1 H NMR), X-ray photoelectron spectroscopy (XPS), and FTIR. The laccase and platinum nanozyme exhibited the synergies during catalyzing the oxidation of EC. CF@PEC exhibits good photothermal effect (photothermal temperature of 60.3°C), UV protection ability (UPF of 95.6), and antioxidant properties (94.4 ± 0.3%). In addition, CF@PEC can maintain UV protection ability after undergoing 20 washing cycles. Notably, the Lac-Pt used to create the multifunctional coating not only preserves the strength of the cotton fabric, but also reduces wastewater production. Briefly, this work renders a promising strategy to prepare the protective coating, which may open a sustainable and efficient avenue to room-temperature thermal therapy and can provide heat to the body in cold environments.
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Advanced Science
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2025
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IF 14.1
Xingdie Zhou,Zhendong Lv,Zehao Chen,Yiming Xu,Chao Lin,Li Liu,Hao Chen,Bing Niu,Wenguo Cui,Yuhui Zhang
Disruption of low oxygen tension homeostasis during intervertebral disc degeneration inhibits endogenous stem cell viability and function, posing a challenge for endogenous regeneration. Here, to achieve sustained hypoxia manipulation, constructed hypoxia-inducible interpenetrating polymer network (IPN) hydrogel microspheres (HIMS) are constructed by microfluidics to integrate the hypoxic system with a stabilizing network. The IPN is synthesized through a two-step polymerization process, consisting of rapid photo-crosslinked gelatin methacrylate anhydride (GM) polymer I and slow enzyme-crosslinked vanillin-grafted gelatin (GV) polymer II. The enzymatic reaction between GV and laccase is able to create a hypoxic microenvironment to modulate oxygen tension in situ within the injured region. HIMS can reduce microenvironmental oxygen tension by 1/3 and maintain a hypoxic microenvironment for up to 5 days, thereby activating the PI3K/AKT/HIF-1α signaling pathway in endogenous stem cells to promote differentiation into nucleus pulposus-like cells. Additionally, NSC-Exos are loaded onto HIMS to trigger endogenous progenitor/stem cell recruitment and migration. Both in vitro and in vivo assays demonstrate that NSC-Exos@HIMS facilitates stem cell recruitment, targets differentiation, and stimulates extracellular matrix synthesis. Overall, the microspheres established herein provide a novel strategy for manipulating oxygen tension and enhancing endogenous tissue regeneration in injured regions during intervertebral disc degeneration.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Jiachun Wang,Juntao Hu,Wantong Jiang,Dongwei Zhu,Yinuo Yin,Yu Qin,Ao Song,Yibing Huang,Quan Luo
Photoconvertible fluorescence probes are indispensable tools for rapid and accurate tissue diagnosis. Herein, we developed peptide-coated gold nanoclusters (AuNCs) capable of fully reversible red/green fluorescence switching. Mechanistic studies revealed that the photoconversion behavior of AuNCs is dictated by the interdependent effects of Au valencies, tyrosine phenol forms, and the interactions between Lys/Arg amino groups and the gold cores. This fluorescence switching involves two key processes: (1) a gradual red-to-green shift regulated by a slow reduction reaction that modulates the Au(I)/Au(0) ratio and phenol oxidative states; (2) an instantaneous green-to-red transition triggered by mildly acidic pH, which promotes protonation of phenol and amino groups, thereby disrupting their interactions with the gold cores. Due to their high pH sensitivity, these AuNCs exhibit distinct and cell-type-specific fluorescence responses, allowing unambiguous discrimination between normal and tumor cells based on intracellular pH differences. In vivo experiments further confirmed the probe's ability to selectively and rapidly delineate tumor tissues, highlighting its potential as a fluorescence-guided navigation tool for minimally invasive cancer surgery.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Guolin Zheng,Zhe Jiang,Yifan Cui,Man Zhou,Yuanyuan Yu,Ping Wang,Qiang Wang
Multifunctional textiles have attracted widespread attention with the improvement of awareness of health. Especially, the fluorine-free superhydrophobic and conductive cellulose fiber-based fabrics have received intensive interest due to their broad and high-value applications. Herein, the copper sulfide nanoflowers were in-situ deposited on cotton fabric followed by polydimethylsiloxane (PDMS) treatment for encapsulating CuS nanoflowers and obtaining superhydrophobicity, recorded as Cot@PTA@CuS@PDMS. Cot@PTA@CuS@PDMS possesses superhydrophobicity with contact angles of 153.0 ± 0.4°, photothermal effect, excellent UV resistance, good conductivity, and anti-fouling. Interestingly, the resistance of Cot@PTA@CuS@PDMS is significantly reduced from 856.4 to 393.1 Ω under simulated sunlight irradiation with 250 mW/cm 2 . Notably, the resistance can be slightly recovered after shutting off simulated sunlight. Besides, Cot@PTA@CuS@PDMS has efficient oil-water separation efficiency for corn germ oil and castor oil, respectively. Briefly, this work provides a novel, facile, and promising strategy to fabricate multifunctional fiber-based textiles with the reversible change of resistance under simulated sunlight irradiation, inspiring more scholars to control the resistance change of textiles by light irradiation.
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