Animals
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2023
·Vol. 13
·期 3
·345
·
IF 3
Hulong Lei,Qian Du,Naisheng Lu,Xueyuan Jiang,Mingzhou Li,Dong Xia,Keren Long
Simple SummaryCopper sulfate and copper glycinate have been used as additives for copper supplementation and growth promotion in the swine feed industry. However, their effects on the gut microenvironment, especially for the fecal microbiota and metabolites, still remain unclear, which are the important indicators of gut health. As well as the nutrient digestibility and physiological and biochemical indices of pigs, the effects of copper sulfate and copper glycinate on fecal microbial communities and metabolic profiles were investigated to understand it better in the present study. Our results suggested that copper sulfate and copper glycinate could differentially affect fecal microbiota and metabolites, by which dietary copper glycinate exerted more beneficial effects on the gut health of pigs.AbstractThis study aims to compare the fecal microbiome-metabolome response to copper sulfate (CuSO4) and copper glycinate (Cu-Gly) in pigs. Twelve Meishan gilts were allocated into the CuSO4group and the Cu-Gly group (fed on a basal diet supplemented with 60 mg/kg copper from CuSO4or Cu-Gly) paired in litter and body weight. After a two-week feeding trial, the Cu-Gly group had a higher copper digestibility, blood hemoglobin, and platelet volume and higher levels of plasma iron and insulin-like growth factor-1 than the CuSO4group. The Cu-Gly treatment increased the abundance of theLachnospiraceaefamily and the generaLachnospiraceae XPB1014,Corprococcus_3,Anaerorhabdus_furcosa_group,Lachnospiraceae_FCS020_group, andLachnospiraceae_NK4B4_groupand decreased the abundance of theSynergistetesphylum andPeptostreptococcaceaefamily compared to the CuSO4treatment. Moreover, the Cu-Gly group had a lower concentration of 20-Oxo-leukotriene E4 and higher concentrations of butyric acid, pentanoic acid, isopentanoic acid, coumarin, and Nb-p-Coumaroyl-tryptamine than the CuSO4group. The abundance ofSynergisteteswas positively correlated with the fecal copper content and negatively correlated with the fecal butyric acid content. The abundance of theLachnospiraceae_XPB1014_groupgenus was positively correlated with the plasma iron level and fecal contents of coumarin and butyric acid. In conclusion, Cu-Gly and CuSO4could differentially affect fecal microbiota and metabolites, which partially contributes to the intestinal health of pigs in different manners.Keywords:copper sulfate;copper glycinate;microbiome;metabolome;pigs;copper source
研究领域:
动物科学
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营养学
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微生物学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Water
·
2021
·Vol. 13
·期 24
·3497
·
IF 3.103
Zhongda Hu,Zijing Fan,Qixuan Song,Rabia Khatoon,Mei Zhang,Ning Wang,Xingzhang Luo
Tetracycline antibiotics (TCs) are a common type of antibiotic found in swine wastewater. Oxytetracycline (OTC) is a significant type of TC. This study mainly examined the influence of OTC on high-temperature anaerobic digestion by adding OTC to collections of swine wastewater at different times during the digestion process. The results showed that high-temperature anaerobic digestion was suitable for the removal of TCs, with an 87% OTC removal efficiency achieved by day 20. Additionally, OTC added from external sources was found to inhibit the chlortetracycline degradation process and affect the first-order degradation kinetic model of TCs. Complexation reactions were the main ways in which OTC affected the heavy metal content of the water. The exogenous addition of OTC was found to inhibit the activity of some digester microbial strains, reduce the proportion of dominant strains, such asMBA03, and kill certain specific strains. This performance alteration was most obvious when OTC was added in the middle of the reaction.Keywords:swine wastewater;high-temperature anaerobic digestion;tetracycline antibiotics;oxytetracycline
研究领域:
环境工程
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微生物学
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DOI
高影响因子
ENERGY
·
2025
·
IF 9.4
Xiaomin Wu,Zhiming Huang,Jingwei Chen
This work investigated the hydrothermal degradation of typical antibiotics present in swine manure (SW) under varying reaction parameters and evaluated the potential of hydrothermal carbonization (HTC) products as high-value carbon materials and liquid fertilizers. The results indicate that doxycycline, sulfadiazine and enrofloxacin were completely degraded under the HTC condition of 260 °C and 40 min, while the optimal HTC conditions for SW were identified as 300 °C, 40 min, and a solid-liquid ratio of 0.1 g/mL. Analysis of the HTC products revealed that hydro-char exhibited favorable surface morphology, a high degree of carbonization, the ability to stabilize heavy metals, a high higher heating value (HHV), and excellent thermal stability. Furthermore, the hydrothermal wastewater complies with discharge standards in terms of heavy metal content, antibiotic concentration, and drug-resistant bacteria content. It also contains significant amount of nitrogen and potassium, along with moderate phosphorus content, highlighting its potential as a liquid fertilizer. This study provides novel insights into the harmless and resourceful utilization of SW and can also serve as a valuable reference for the treatment of other organic waste.
研究领域:
环境科学
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废弃物资源化
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生物工程
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DOI
高影响因子
JOURNAL OF SURGICAL RESEARCH
·
2012
·
IF 2.247
Jiaoming Dong,Yu Li,Xiumei Mo
Background The use of extracellular matrix (ECM) derived from decellularized tissue is increasingly frequent in regenerative medicine and tissue engineering . However, it is recognized that currently used decellularization procedures have negative effects on ECM integrity. The objective of this study was to investigate the impact of a decellularization protocol with a new detergent on the ECM integrity of porcine pericardium (PP) compared with other traditional detergents. Materials and methods Fresh PP were decellularized by sodium deoxycholate in combination with Triton X-100 (SDT), sodium dodecyl sulfate (SDS), and octyl-glucopyranoside (OGP), respectively. Histologic analysis and scanning electron microscopy were performed to confirm the removal of cells and to examine the structure of ECM. DNA content was examined by the method of DNA extraction . Mechanical properties and biochemical compositions of ECM were also studied. Results Histologic analysis and DNA determination demonstrated that SDS and OGP completely removed the cells, and the major ECM structure was preserved well for PP treated with 1% (wt/vol) OGP but disrupted for PP treated with SDS; whereas treatment with SDT was insufficient to remove cells from PP. Uniaxial tensile tests showed that PP decellularized by OGP had similar mechanical properties to native PP, whereas the mechanical properties of PP decellularized by SDS and SDT decreased. The biochemical compositions of PP decellularized by OGP were also well conserved, except that glycosaminoglycans markedly decreased. Moreover, the results obtained in the MTT study further indicated that the cytotoxicity of PP decellularized by OGP was significantly lower than that decellularized by SDS and SDT. Conclusion It is suggested that the environmentally friendly and nontoxic OGP can be used as a decellularizing agent. The OGP method could achieve both complete removal of cells from native PP and preservation of the matrix structure; thus, it might be a suitable approach to preparation of tissue engineering heart valve scaffold.
研究领域:
再生医学
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组织工程
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生物材料学
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产品关联
DOI
PubMed
高影响因子
Food Packaging and Shelf Life
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2025
·
IF 10.6
Liangliang Zhang,Hanbo Yi,Saisai Feng,Mengmeng Zhang,Yu Wu,Jiahui Hao,Xuefang Liu,Jianguo Xu
Chitosan (CS) and polylactic acid (PLA) are both commonly used packaging materials in the food industry. However, a bilayer film made from CS and PLA, simultaneously incorporated with a complex essential oil-loaded Pickering emulsion (PE) system, have not been widely investigated. Therefore, this study designed bilayer films consisting of PLA layer and a PE layer. In the PE layer, binary nanoparticles composed of soybean protein isolate (SPI) and CS were used as solid supports to load increasing amounts (0 %, 2 %, 4 %, 6 %, 8 %, v/v) of complex essential oils (carvacrol and thymol, CT). The films were labeled BF-CT0, BF-CT2, BF-CT4, BF-CT6, and BF-CT8. The characteristics and functional properties were evaluated, along with the quality changes in refrigerated pork wrapped with the films (BF-CT0 or BF-CT8). Results indicated that CT addition significantly enhanced the stability of the PE. The PE layer with higher CT doses exhibited a denser microstructure, more hydrophobic surface, improved elongation at break, and greater water resistance. CT addition significantly improved light and gas barrier functions. Notably, the BF-CT8 film demonstrated extremely low water vapor and oxygen permeability (1.25 ± 0.06 kg•m⁻¹•s⁻¹•Pa⁻¹ and 0.24 ± 0.01 kg•m⁻¹•s⁻¹•bar⁻¹, respectively). All films showed a similar semi-crystalline structure, and CT addition decreased crystallinity. As expected, BF-CT8 displayed enhanced antioxidant and antibacterial performance. Finally, BF-CT8 extended the shelf life of refrigerated pork from 3 days to 6 days compared to BF-CT0 and unwrapped pork. In conclusion, the bilayer film shows great potential for use in pork preservation.
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
Food Bioscience
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2024
·
IF 4.8
Haoyue Ning,Lixin Lu,Yuemei Zhang
To achieve long-term effective protection of food by continuously controlling the release of active substances in an active film, IRMOF-3 was post-synthetic modified with benzoic acid (BA) to obtain BA-IRMOF-3. This modification enhanced the loading capacity of carvacrol (CA) and slowed down its release rate. Research on IRMOF-3 synthesis has found that increasing the proportion of metal nodes can improve its crystallinity. The post-synthesis modification of IRMOF-3 through amide reaction successfully enhanced the binding energy with CA from −5.1 kcal/mol to −6 kcal/mol, resulting in an improved CA loading rate of 28.62% ± 0.15% and delayed release. Furthermore, the BA-IRMOF-3/CA assembly was added to the sodium alginate matrix to prepare the active film BMC@SA, which improved the mechanical and physical properties of the film. The release and antibacterial/antioxidant properties were investigated. The results demonstrated that the active film BMC@SA exhibited significant sustained-release properties and antibacterial/antioxidant activities, reducing the growth of E. coli and S. aureus by 61.95% and 49.33% respectively. In refrigerated pork experiments, the film BMC@SA slowed down the color, weight, pH changes and inhibited microbial growth of fresh pork effectively, and extended the shelf life of pork from 6 d to 12 d, which demonstrated the potential application of active film BMC@SA in food preservation.
研究领域:
食品科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Current Drug Delivery
·
2021
·Vol. 18
·期 2
·224-233
·
IF 2.565
Xie Shuyu,Zhang Xiaoqiu,Luo Wanhe,Meng Kuiyu,Chen Dongmei,Pan Yuanhu,Tao Yanfei,Huang Lingli,Liu Zhenli,Wang Yulian,Yuan Zonghui
Objective: A ceftiofur hydrochloride long-acting oily suspension with no irritation was prepared by testing and optimizing the types and amounts of organic solvents, suspending agents, and surfactants. Methods: Its properties, stability, injection site irritation, in vitro
release, and pharmacokinetics in pigs were evaluated. The optimum formulation was used ethyl oleate, aluminum monosterate, and span-80 as organic solvents, suspending agents, and surfactant, respectively. The drug microparticles were uniform long strip with size of 1.53 ± 0.11 μm
and no agglomerations, and were evenly dispersed. The re-dispersed time, sedimentation rate and pH value of the suspension were 4 s under a magnetic shaker rotating at 20 r/min, 1 and 5.0, respectively. It could go through 7-gage needle smoothly with withdrawal volume of 9.9 mL/min. Results:
The suspension showed good stability when stored away from light, no irritation at the injection site and sustained release in PBS buffer. After intramuscular administration, the drug concentration above 0.15 μg/mL was last for 120 h. Its elimination half-life (T1/2ke), mean
residence time (MRT), and bioavailability were increased by 1.73, 1.62, and 2.16 times compared to Excenel®. Conclusion: The results suggested that the suspension had excellent sustained-release and will make ceftiofur hydrochloride more effective and convenient to use.
研究领域:
兽医学
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药物制剂
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药理学
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DOI
PubMed
高影响因子
PLoS One
·
2014
·Vol. 9
·期 9
·e108314
·
IF 3.534
Xiang Hou,Jingfei Zhang,Hussain Ahmad,Hao Zhang,Ziwei Xu,Tian Wang
The aim of this study was to investigate the antioxidant potential of ampelopsin (APS) by using various methods in vitro , as well as to determine effects of APS on LPS-induced oxidative stress in piglets. The results showed that APS exhibited excellent free radical scavenging by DPPH, ABTS, O 2 • − , H 2 O 2 and ferric reducing antioxidant power. Ampelopsin also protected pig erythrocytes against AAPH-induced apoptosis and hemolysis, decreased total superoxide dismutase activity, and increased lipid peroxidation. Furthermore the results demonstrated that APS enhanced the total antioxidant capacity and decreased the malondialdehyde and protein carbonyl contents in LPS-treated piglets. The results of the present investigation suggest that APS possesses a strong antioxidant activity and alleviates LPS-induced oxidative stress, possibly due to its ability to prevent reactive oxygen species.
研究领域:
动物医学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2020
·
IF 7.053
Arianna Dick,Bhesh Bhandari,Xiuping Dong,Sangeeta Prakash
The effect of the independent (0.36%) and combined (0.36% in proportion 0.5:0.5, 0.7:0.3, and 0.3:0.7) addition of xanthan gum and guar gum on the rheological, textural, and microstructural properties of 3D printed cooked pork paste was examined. Shear-thinning behaviour and self-supporting ability were observed for all the samples, resulting in upright printing performance. Upon post-processing by freezing at −18 °C, heating at 100 °C for 7 min, and cooling to 37 °C, significant (P < 0.05) textural differences were recorded. The control sample displayed higher hardness, chewiness , and cohesiveness than the samples with added hydrocolloids , elucidated by a lower loss tangent (more elastic-like behaviour), and a denser microstructure observed in the cryo-SEM micrographs. Whereas, the samples with hydrocolloids presented a visible extended network with heterogeneous cavities-size, suggesting additional water retention and a more viscous-like behaviour than the control sample after post-processing, thus categorized as potential transitional foods within the International Dysphagia Diet Standardisation Initiative (IDDSI) Framework.
研究领域:
食品科学
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生物材料学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Qian Wang,Li Zhang,Wu Ding
Chilled pork is widely consumed in China. However, various contaminants during storage directly lead to a decline in the quality of chilled pork products. An extract of natural plant sources, eugenol (Eug) exerts good antibacterial and antioxidant effects. Nanometerization was used in this study to improve the insoluble and volatile characteristics of Eug. Eug nanocapsules embedded with gelatin/chitosan (Eug-Gel-CS NPs) were used to preserve chilled pork. Results indicated that Eug-Gel-CS NPs could effectively inhibit increases in the pH, total volatile basic nitrogen (TVB-N), and thiobarbituric acid-reactive substances (TBARS) of chilled pork than that of the Eug group ( p < 0.05). The L * and a * values of the Eug-Gel-CS NPs group were significantly higher than those of the Eug and gelatin-chitosan (CS-Gel) groups ( p < 0.05). The total number of colonies (TBC) showed that the storage period of the Eug-Gel-CS NPs group could be extended to 15 d, which was significantly different from that of the CK group (8 d) ( p < 0.05). The Eug-Gel-CS NPs also effectively delayed the decline in the water- holding capacity (WHC), springiness, and cohesiveness of the chilled pork. Therefore, Eug-Gel-CS NPs exert good antiseptic, antibacterial, and antioxidative effects on preserved chilled pork.
研究领域:
食品科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Leiyu Feng,Xuyao Li,Xutao Chen,Yujun Huang,Kangshou Peng,Yuxuan Huang,Yuanyuan Yan,Yinguang Chen
Ordered nitrogen-doped mesoporous carbon (NMC) was successfully synthesized with pig manure as the precursor. The resulting NMC materials exhibited excellent capacity of adsorption and potassium persulfate (PS) activation when used as catalysts for the oxidative degradation of tetracycline antibiotics (tetracycline hydrochloride (TH) as the target). For an initial TH concentration of 35 mg/L, the maximum adsorption capacity of NMC material prepared at 700 °C (NMC700) was 122.0 mg/g, and the degradation efficiency in the PS reaction system was 94.8% within 120 min. Investigation of the mechanism indicated that the NMC700 material with specific surface area (SSA) of 275.5 m 2 /g and 0.7% graphitic N content, provided a large amount of active sites for adsorption and catalytic oxidation of TH. Based on the results of selective degradation and electron paramagnetic resonance (EPR) experiments, a non-radical pathway for the degradation of pollutants was proposed. Chronoamperometry evaluation also supported the conclusion that the NMC material enhanced electron transfer to activate persulfate, accelerating the removal of TH.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2016
·Vol. 97
·期 7
·2023-2029
·
IF 2.076
Biao Suo,Huarong Li,Yuexia Wang,Zhen Li,Zhili Pan,Zhilu Ai
BACKGROUND There has been limited research on the use of ZnO nanoparticle-coated film for the quality preservation of pork meat under low temperature. In the present study, ZnO nanoparticles were mixed with sodium carboxymethyl cellulose (CMC-Na) to form a nanocomposite film, to investigate the effect of ZnO nanoparticle-coated film on pork meat quality and the growth of bacteria during storage under low temperature. RESULTS When ZnO nanoparticle-coated film was used as the packaging material for pork meat for 14 days of cold storage at 4 °C, the results demonstrated a significant effect on restricting the increases in total volatile basic nitrogen and pH levels, limiting the decreases of lightness (increased L * value) and redness (increased a * value), and maintaining the water-holding capacity compared to the control pork samples ( P < 0.05). The present study also discovered that the ZnO nanoparticle-coated film restrained the increase in total plate count (TPC). When Staphylococcus aureus was used as the representative strain, scanning electron microscopy revealed that ZnO nanoparticles increased the occurrence of cell membrane rupture under cold conditions. CONCLUSION ZnO nanoparticle-coated film helps retain the quality of pork meat during cold storage by increasing the occurrence of microorganism injury. © 2016 Society of Chemical Industry
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）