Biotechnology Journal
·
2025
·Vol. 20
·期 8
·e70096
·
IF 3.1
Xueqin Fu,Zhiqin Zhu,Peipei Yin,Yi Yang,Yunhong Huang,Zhong-er Long,Zhiming Wu,Long Zou,Haiyan Ni
Microbial reduction of toxic Se(IV) oxyanions to biogenic Se(0) has garnered considerable attention for detoxification. This study presents a comprehensive investigation of Se(IV) reduction by environmentally versatile Exiguobacterium genus through integrated physicochemical, genomic, and transcriptomic analyses. Exiguobacterium mexicanum PY14 demonstrated remarkable efficiency, reducing ∼1 mM selenite to extracellular Se(0) within 12 h under aerobic conditions, with broad adaptability to pH (7–9), temperature (30–37°C), and salinity (up to 40 g L −1 NaCl). The produced Se(0) revealed crystalline nanoaggregates with biomolecular coatings. Genomic sequencing identified a chromosome and six plasmids enriched with genes for carbohydrate metabolism, inorganic ion transport, and mobile genetic elements. Transcriptomic profiling under Se(IV) stress unveiled a coordinated stress response: up-regulation of catabolic pathways (glycolysis and citric acid cycle) for energy and NAD(P)H production, bacterial motility, and chemotaxis, alongside down-regulation of energy-intensive biosynthetic processes. Notably, genes for glutathione biosynthesis ( gsh ), NAD(P)H generation ( gntZ ), and ROS scavenging ( btuE ) were significantly up-regulated, along with the evidence of increased GSH levels, implicating a GSH-dependent detoxification pathway driving Se(IV) reduction. These findings deepen mechanistic understanding of Se(IV) reduction mechanism within the understudied Exiguobacterium genus, and the strain's haloalkaliphilic trait underscores its potential for bioremediating Se(IV)-contaminated saline-alkaline environments.
研究领域:
环境微生物学
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生物修复
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微生物生理学
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基因组学
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转录组学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Kai Zhang,Xiaoqing Guo,Qingyu Zhao,Yunsheng Han,Tengfei Zhan,Ying Li,Chaohua Tang,Junmin Zhang
Dietary selenium deficiency is recognized as a global problem. Pork is the most widely consumed meat throughout the world and an important source of selenium for humans. In this study, a reliable approach was developed for analyzing selenium and its speciation in the muscles of pigs after different selenium treatments. The selenium source deposition efficiency was ranked as: selenomethionine > methylselenocysteine > selenite, and the muscle selenium content had a dose effect with selenomethionine supplementation. In total, four species of selenium were detected in the muscles of pigs and the distributions of these selenium species were greatly affected by the dietary selenium supplementation forms and levels. Selenomethionine (>70% of total selenium) and selenocystine (>11%) were the major selenium species, followed by methylselenocysteine and selenourea. Therefore, selenium-enriched pork produced from selenomethionine is a good source for improving human dietary selenium intake.
研究领域:
分析化学
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营养学
·
动物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Yinglei Han,Tongqing Sun,Hongde Liu,Dahuai Zheng,Shiguo Liu,Yongfa Kong,Jingjun Xu
Due to the outstanding performance of iodate systems in the field of nonlinear optics, many iodate compounds have been synthesized. However, exploring novel iodate nonlinear optical (NLO) crystals remains challenging. Base on the mixed anionic groups strategy, we successfully synthesized Ln(H 2 O) 2 (IO 3 )(SeO 4 )·H 2 O (Ln = Y, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu) crystals by the hydrothermal redox reaction. These compounds crystallize in the P 2 1 2 1 2 1 space group of orthorhombic system, and exhibit NLO response. However, their second-harmonic generation (SHG) efficiencies were much weaker than that of KDP due to the reverse arrangement of the anionic groups. Furthermore, the structural adjustment was conducted by the substitution of Ln³⁺ by Al³⁺, which resulted in the discovery of a new compound — [Al(OH) 2 ] 3 (IO 3 )(SeO 4 )·H 2 O. Despite the significant variation in the radii of the substituted cations, the aluminum iodate-selenate retains the structural framework of the original crystals. Moreover, it crystallizes in an NCS space group with higher symmetry — P 6 3 mc . The experiments showed that its SHG efficiency increased to 2 × KDP as a consequence of structural modification. Detailed structural analyses and theoretical calculations indicate that the orientational consistency of the [IO 3 ] − anion groups is the key factor contributing to the enhancement of the SHG response. This work offers new candidate materials and design strategies for the development of novel nonlinear optical materials with high-performance.
研究领域:
无机化学
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材料科学
·
晶体工程
·
非线性光学
·
固体化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Jinling Cui,Mingzhu Yan,Xiaoyi Liu,Shutao Yin,Shangyun Lu,Lihong Fan,Hongbo Hu
Previous studies have shown that selenite, a representative of inorganic form selenium, exerts its anticancer effect by inducing apoptosis in androgen-dependent LNCaP prostate cancer cells, but few studies have determined the nature of cell death induced by selenite in metastatic androgen-refractory PC-3 cells. Our study showed that necrosis-like cell death rather than apoptosis, pyroptosis, or autophagic cell death was caused by selenite in PC-3 cells. Mechanistically, this type of cell death was caused by ATP depletion (26.28 ± 3.39 nmol/mg of control versus 9.12 ± 2.44 nmol/mg of 10 μM selenite treatment) that resulted from phosphofructokinase activity reduction (100.17 ± 0.17% of control versus 21.74 ± 6.65% of 10 μM selenite treatment). Our study also showed that ROS production is necessary for the decrease in cellular ATP levels and in phosphofructokinase activity. To our knowledge, this is the first study showing that selenite can induce necrosis-like cell death in PC-3 cells. Our findings support selenite as an effective compound for the therapy of apoptosis-resistant prostate cancer.
研究领域:
肿瘤学
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细胞生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2016
·
IF 2.857
Guang Wang,Liying Wu,Hong Zhang,Wenjia Wu,Mengmeng Zhang,Xiaofeng Li,Hui Wu
To clarify the mechanisms of selenium (Se) tolerance in peanut seedlings, we grew peanut seedlings with sodium selenite (0, 3, and 6 mg/L), and investigated the phenylpropanoids metabolism in seedling roots. The results showed that selenite up-regulated the expression of genes and related enzyme activities involving in the phenylpropanoids biosynthesis cascade, such as phenylalanine ammonia-lyase, trans-cinnamate-4-hydroxylase, chalcone synthase, chalcone isomerase, and cinnamyl-alcohol dehydrogenase. Selenite significantly increased phenolic acids and flavonoids, which contributed to the alleviation of selenite-induced stress. Moreover, selenite enhanced the formation of endodermis in roots, which may be attributed to the up-regulation of lignin biosynthesis mediated by the selenite-induced changes of H2O2 and NO, which probably regulated the selenite uptake from an external medium. Accumulation of polyphenolic compounds via the phenylpropanoid pathway may be one of the mechanisms of the increasing selenite tolerance in plants, by which peanut seedlings survived in seleniferous soil, accompanied by accumulation of Se.
研究领域:
植物生理学
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环境胁迫响应
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2020
·
IF 4.825
Yun-Xiang Ma,Ya-Ping Gong,Chun-Li Hu,Fang Kong,Jiang-Gao Mao
The search of novel second-harmonic generation crystals in gallium selenite systems results in three new compounds of MGa2(SeO3)4 (M = Sr, Pb) and BiGa(SeO3)3. The isostructural MGa2(SeO3)4 (M = Sr, Pb) are centrosymmetric (P1̅, no. 2). The structures represent a complicated three-dimensional skeleton containing two different types of Ga4Se4 8-member polyhedral ring tunnels with the counter cations located at the broad ones. When the divalent counter cations were substituted by a trivalent lone pair cation of Bi(III), a novel noncentrosymmetric compound of BiGa(SeO3)3 was achieved. BiGa(SeO3)3 displays a 3D gallium selenite framework with 1D bismuth oxide chains filling in the 12-member polyhedral ring tunnels. Frequency doubling measurements showed that BiGa(SeO3)3 presented an apparent SHG signal comparable to the commercial KDP and can realize phase matching. The lone pair anionic groups of SeO32– made the major contribution according to the SHG density analyses. BiGa(SeO3)3 exhibits a wide transparent range and large optical bandgap (3.90 eV). The laser-induced damage threshold of BiGa(SeO3)3 was estimated to be 98.68 MW/cm2, about 44 × AGS.
研究领域:
材料科学
·
光学
·
晶体学
✓ 已验证
DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2018
·
IF 2.179
Yun-Xiang Ma,Ya-Ping Gong,Chun-li Hu,Jiang-Gao Mao,Fang Kong
Three new d 10 transition metal selenites containing PO 4 tetrahedron, namely, Cd 7 (HPO 4 ) 2 (PO 4 ) 2 (SeO 3 ) 2 ( 1 ), Cd 6 (PO 4 ) 1.34 (SeO 3 ) 4.66 ( 2 ) and Zn 3 (HPO 4 )(SeO 3 ) 2 (H 2 O) ( 3 ), have been synthesized by hydrothermal reaction . They feature three different structural types. Compound 1 exhibits a novel 3D network composed of 3D cadmium selenite open framework with phosphate groups filled in the 1D helical tunnels. The structure of compound 2 displays a new 3D framework consisted of 2D cadmium oxide layers bridged by SeO 3 and PO 4 groups. Compound 3 is isostructural with the reported solids of Co 3 (SeO 3 ) 3-x (PO 3 OH) x (H 2 O) when x is equal to 1.0. Its structure could be viewed as a 3D zinc oxide open skeleton with SeO 3 and HPO 4 polyhedra attached on the wall of the tunnels. They represent the only examples in metal selenite phosphates in addition to the above cobalt compounds. Optical diffuse reflectance spectra revealed that these solids are insulators, which are consistent with the results of band structure computations based on DFT algorithm.
研究领域:
无机化学
·
材料科学
·
固体化学
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2021
·
IF 12.479
Si-Yuan Peng,Xin-Hua Liu,Qi-Wen Chen,Yun-Jian Yu,Miao-Deng Liu,Xian-Zheng Zhang
The overexpression of glutathione (GSH) in cancer cells has long been regarded as the primary obstacle for reactive oxygen species (ROS)-involved anti-tumor therapies. To solve this issue, a ferric ion and selenite-codoped calcium phosphate (Fe/Se–CaP) nanohybrid here is fabricated to catabolize endogenous GSH, instead of directly deleting it, to trigger a ROS storm for tumor suppression. The selenite component in Fe/Se–CaP can catabolize GSH to superoxide anion (O 2 •− ) and hydroxyl radicals (•OH) via cascade catalytic reactions , elevating oxidative stress while destroying antioxidant system. The doped Fe can further catalyze the soaring hydrogen peroxide (H 2 O 2 ) originated from O 2 •− to •OH via Fenton reactions. Collectively, Fe/Se–CaP mediated self-augmented catabolism dynamic therapy finally induces apoptosis of cancer cells owing to the significant rise of ROS and, combined with CaP adjuvant, evokes adaptive immune responses to suppress tumor progression, providing an innovative train of thought for ROS-involved anti-tumor therapies.
研究领域:
纳米技术
·
癌症治疗
·
生物化学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY OF MATERIALS
·
2025
·
IF 7
Yurui Wang,Xuehua Dong,Wei Zeng,Ling Huang,Zhien Lin,Hongmei Zeng,Guohong Zou
Designing nonlinear optical (NLO) materials that simultaneously exhibit enhanced second-harmonic generation (SHG), moderate birefringence (Δn), and short-wavelength ultraviolet (UV) transparency remains a significant challenge. This study presents a stepwise structural regulation strategy using the International Zeolite Association (ABW)-type zeolite NH4BePO4 (I) as the parent material. By systematically modulating the anionic units, we successfully synthesized two compounds: KBe2(HPO3)2F (II) and NH4Be2(OH)(SeO3)2·H2O (III). Compound III represents a breakthrough, demonstrating an SHG intensity 4.0 times greater than that of KH2PO4, a birefringence of 0.080 at 546 nm, and a UV cutoff at 203 nm. The enhanced optical anisotropy and NLO efficiency of compound III arise from the stereochemically active lone pairs in the [SeO3] groups. By optimizing SHG intensity, short-wavelength UV transparency, and birefringence simultaneously, compound III addresses the key challenge of designing high-performance short-wavelength UV NLO materials. This work not only validates the stepwise regulation strategy but also provides a design framework for achieving balanced NLO performance, paving the way for the development of next-generation optical materials.
研究领域:
材料科学
·
非线性光学
·
无机化学
·
晶体工程
·
光学材料
✓ 已验证
产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2020
·Vol. 49
·期 40
·14046-14051
·
IF 4.174
Huanke Zhao,Pifu Gong,Xinyuan Zhang,Zheshuai Lin,Zhanggui Hu,Yicheng Wu
Two new isomorphic selenite bromides, namely Pb2GaF2(SeO3)2Br (1) and Pb2NbO2(SeO3)2Br (2), were synthesized by mild hydrothermal reactions. These two compounds crystallize in the noncentrosymmertric space group P21 and feature 3D structures consisting of 1D (Pb2Br)3+ cationic chains and 2D [Ga2F4(SeO3)4]6−/[Nb2O4(SeO3)4]6− anonic chains. As measured, 1 and 2 are phase-matchable and exhibit excellent second harmonic generation (SHG) responses of 4.5 and 1.4 times that of KDP, respectively. Moreover, these two compounds possess wide bandgaps (>3.17 eV) and high thermal stabilities (>425 °C), indicating their suitable performances as potential nonlinear optical materials. First-principle density functional theory (DFT) calculations were also performed to explicate the structure–property relationship.
研究领域:
材料科学
·
非线性光学
·
晶体学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Shuojia Liang,Junlei Yu,Meng Zhao,Sha Chen,Xiang Lu,Fayin Ye,Jia Chen,Guohua Zhao,Lin Lei
Selenium bioavailability is critically influenced by gut microbiota, yet the interaction dynamics with selenocompounds remain unexplored. Our study found that L-Selenomethionine (SeMet) and Se-(Methyl)seleno-L-cysteine (MeSeCys) maintained stability during in vitro gastrointestinal digestion. In contrast, Selenite and L-Selenocystine (SeCys 2 ) were degraded by approximately 13% and 35%. Intriguingly, gut microflora transformed MeSeCys, SeCys 2 , and Selenite into SeMet. Moreover, when SeCys 2 and Selenite incubated with gut microbiota, they produced red selenium nanoparticles with diameters ranging between 100 and 400 nm and boosted glutathione peroxidase activity. These changes were positively associated with an increased relative abundance of unclassified_g__Blautia (Family Lachnospiraceae ), Erysipelotrichaceae_UCG-003 (Family Erysipelatoclostridiaceae ), and uncultured_bacterium_g__Subdoligranulum (Family Ruminococcaceae ). Our findings implied that differential microbial sensitivities to selenocompounds, potentially attributable to their distinct mechanisms governing selenium uptake, storage, utilization, and excretion.
研究领域:
营养学
·
微生物学
·
生物化学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）