ANALYTICA CHIMICA ACTA
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2019
·
IF 5.256
Huili Tian,Nuo Duan,Shijia Wu,Zhouping Wang
Spermine contamination ranks as one of the food safety issues, it will cause some adverse reactions if the intake of spermine is excessive in human body. So it is of great significance to establish fast and efficient analysis method to detect spermine in foods. In this study, the spermine aptamers with high affinity and specificity were obtained by the capture systematic evolution of ligands by exponential enrichment (Capture-SELEX) technique. Forty-one aptamer sequences were obtained by cloning and sequencing, and were divided into eight families based on homology and secondary structure analysis. The affinity and specificity of candidate aptamers was analyzed by isothermal titration calorimetry (ITC) and fluorescence assay . The aptamers named APJ-6 was picked out as the optimal aptamer that recognizes spermine specifically with the Kd value of 9.648 ± 0.896 nM. In order to verify the practicability of the selected aptamers, the sensitive aptamer-based fluorescene assay was designed. Under optimized conditions, this aptasensor exhibited a low detection limit of 0.052 nM, as well as a linear within the range of 0.1–20 nM. Besides, it has been further applied for the determination of spermine in pork samples and the recoveries ranged from 86.45% to 98.15%, showing its great potential for sensitive analysis in food safety control.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2019
·Vol. 136
·期 45
·48186
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IF 2.188
Xiaoguang Ying,Anshun Kang,Xiaomei Zhu,Xiao Li
Electrospinning is an important tool for generating continuous fibers with large surface area and high pore ratio. The combination of nanofiber membrane and molecular imprinting can effectively measure specific adsorption of putrescine with coloring procedure. In this work, molecularly imprinted electrospun chromogenic membrane was synthesized with 1,4-butanediol (BDO) as imitate template. Poly(vinyl alcohol) (PVA) is used as spinning solution and ninhydrin as chromogenic agent for visualizing detection of target molecular putrescine. The adsorption concentration on molecularly imprinted polymer is illustrated by blue-purple colored stains and recorded by interactive three-dimensional surface plot of ImageJ. The results indicated that a uniform and bead-free PVA nanofiber was obtained using 10% (w/w) PVA solution, 15 kV voltage, 15 cm spinning distance, and 600 rpm roller speed. Also, the adsorption performance of molecular imprinting chromogenic membrane was investigated in detail, showing a higher adsorption capacity of 33.16 μg cm −2 and maximum imprinting efficiency of 2.30 under the best recognition conditions of 315 μL ninhydrin, 0.25% (w/w) BDO, and 0.4 cm sensing distance, as well as a short adsorption equilibrium time of 40 min. In addition, three amine competitors were discussed to reveal the better selectivity and higher specific recognition ability in the imprinted membrane. HIGHLIGHTS The molecular imprinting and coloring structure is constructed in nanofiber membrane with an enhanced specific surface area. The adsorption on imprinted membrane is visualized by chromogenic reaction between putrescine and ninhydrin to form a three-dimensional interactive surface proceeded using the ImageJ software. The imprinted nanofiber membrane with large area and high pore ratio is applied in detecting target in trace concentration within shortened time. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 48186.
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Food Chemistry-X
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2024
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IF 6.5
Xueli Wang,Xueting Liu,Chunmei Sun,Yanwei Cheng,Zhen Li,Shuyi Qiu,Yongguang Huang
Daocai is a traditional salted pickle in the southeastern region of Guizhou with a unique aroma, color, and taste. The quality of Daocai is greatly influenced by the fermentation temperature. In this study, high-throughput sequencing and headspace-gas chromatography-ion mobility spectrometry were used to investigate the changes in microbial community succession and volatile flavor compounds during Daocai fermentation under temperature-controlled (D group) and non-temperature-controlled (C group).We found that the predominant genera in the C group samples were Latilactobacillus (40.57 %), Leuconostoc (21.25 %), Cystofilobasidium (22.12 %), Vishniacozyma (23.89 %), and Leucosporidium (24.95 %), whereas Weissella (29.39 %), Lactiplantibacillus (45.61 %), Mucor (68.26 %), and Saccharomyces (23.94 %) were the predominant genera in the D group. A total of 92 VFCs were detected in Daocai samples, including 5 isothiocyanates, 16 esters, 14 alcohols, 24 aldehydes, 17 ketones, 3 acids, 2 pyrazines, 1 pyridines, 1 thiazoles, 3 furans, 4 alkenes, and 2 nitriles. Further analysis revealed Latilactobacillus , Leuconostoc , Lactococcus , Cystofilobasidium , Leucosporidium , Holtermanniella , and Dioszegia as key bacteria involved in flavor formation. They are closely related to the formation of flavors such as aldehydes, furans, pyridines, and alkenes. This study contributes to our understanding of the relationship between bacterial communities and the flavor formation during Daocai fermentation.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 2.6
Wanli You,Si Chen,Jinglin Zhang,Xueyin Ru,Feng Xu,Zhengguo Wu,Peng Jin,Yonghua Zheng,Shifeng Cao
Summary The effects of ultrasound (US) pretreatment combined with drying process on storage quality maintenance and bioactive compound accumulation of broccoli stems were investigated. Results indicated that US pretreatment (λ = 8.5 mm) delayed the decline of L* value and ascorbic acid content but inhibited the increase of a* and b* values, browning index, and microbial counts of fresh-cut broccoli stems. Furthermore, the US application enhanced total phenolics content by activating phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, and 4-coumarate-CoA ligase in phenylpropane metabolism. Meanwhile, it also promoted γ-aminobutyric acid (GABA) accumulation by upregulating activities of diamine oxidase, polyamine oxidase, and aminoaldehyde dehydrogenase in GABA shunt and polyamine degradation pathway, respectively, which in turn, accelerated the biosynthesis of GABA from polyamines and glutamate. In addition, compared with other three drying methods, the combined vacuum freeze drying (VFD) could further retain the sensory attributes and bioactive compounds to a maximum extent in dehydrated broccoli stems. Hence, current findings illustrated that US pretreatment combined with VFD process is beneficial for commercial quality maintenance and nutritional value enhancement, thus providing new perspectives on the comprehensive utilisation of broccoli stems.
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MOLECULES
·
2024
·Vol. 29
·期 11
·2461
·
IF 4.2
Wei He,Xinya Zhang,Yangxiaoyu Zou,Ji Li,Chong Wang,Yucai He,Qiuheng Jin,Jianren Ye
mRNA vaccines are entering a period of rapid development. However, their synthesis is still plagued by challenges related to mRNA impurities and fragments (incomplete mRNA). Most impurities of mRNA products transcribed in vitro are mRNA fragments. Only full-length mRNA transcripts containing both a 5′-cap and a 3′-poly(A) structure are viable for in vivo expression. Therefore, RNA fragments are the primary product-related impurities that significantly hinder mRNA efficacy and must be effectively controlled; these species are believed to originate from either mRNA hydrolysis or premature transcriptional termination. In the manufacturing of commercial mRNA vaccines, T7 RNA polymerase-catalyzed in vitro transcription (IVT) synthesis is a well-established method for synthesizing long RNA transcripts. This study identified a pivotal domain on the T7 RNA polymerase that is associated with erroneous mRNA release. By leveraging the advantageous properties of a T7 RNA polymerase mutant and precisely optimized IVT process parameters, we successfully achieved an mRNA integrity exceeding 91%, thereby further unlocking the immense potential of mRNA therapeutics.
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FOOD CHEMISTRY
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2024
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IF 8.5
Yanan Liu,Jiacheng Gan,Fei Chen,Jian Chen,Huan Li,Yanbo Wang
Dual-enzyme co-embedded materials have shown high potential for achieving efficient detection due to the convenience of two-enzyme cascade reactions. Herein, we developed a dual-enzyme hybrid microsphere (HM) based biosensor to detect diamines (histamine was included for ease of description) in aquatic products. The HM was made from diamine oxidase , horseradish peroxidase , and copper phosphate through the biomineralization method. Under optimal conditions, the system displayed linear color response to histamine of different concentrations ranging from 0 to 200 μg/mL. The detection limit of histamine was 0.15 μg/mL, showing higher sensitivity than the two-step free enzyme assay . Moreover, the detection system exhibited good specificity to diamines. The method was used to detect diamines in commercial samples, and the results were compared with those measured by the high-performance liquid chromatography method. Overall, the proposed assay exhibited high potential in diamine quantification and was readily extended to other cascade enzymatic reaction-based detection strategies.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2024
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IF 8.2
Lian Xia,Feng Luo,Xiaojuan Niu,Yue Tang,Yuangen Wu
Pb(II) is a prevalent heavy metal ion classified as a 2B carcinogen. Excessive intake of Pb(II) in the human body can damage the central nervous system, kidneys, liver, and immune system, leading to permanent brain damage, anemia, and cancer. Colorimetry can be applied to rapidly determine Pb(II) residues, but there are still many challenges in the accuracy and sensitivity of detection. Based on the inhibitory impact of Pb(II) on the oxidase-like activity of octahedral silver oxide (Ag 2 O), a colorimetric sensor with smartphone-assisted analysis for the Pb(II) detection was first developed. Herein, it has been found that Pb(II) can adsorb onto the surface of octahedral Ag 2 O, hindering the production of O 2 − in the reaction system. This ultimately results in the suppression of oxidase-like activity, leading to a lighter purple appearance of the colorimetric reaction solution. The sensor exhibits a high degree of sensitivity and a limit of detection (LOD) for Pb(II) was calculated as 2.2 μg L −1 . Hence, the developed colorimetric sensor with high sensitivity, excellent specificity, and high tolerance to sodium ions is hopeful to have practical applications in Pb(II) detection in environmental water samples. Moreover, the sensor will provide a novel strategy for heavy metal ion detection and other substances.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Nian Shi,Wenxuan Li,Yuwei Liu,Shumei Yan,Xia Xu,Di Chen
The determination of biogenic amines (BAs) in alcoholic beverages is crucial for assessing their health impact, ensuring beverage quality, and guaranteeing safety. Herein, a rapid one-pot derivatization/magnetic solid-phase extraction (OPD/MSPE) method was proposed using 6-aminoquinolinyl-N-hydroxysuccinimide carbamate as the derivatization reagent and magnetic hydroxyl-functionalized multi-walled carbon nanotubes as the extraction material. Integration of derivatization and extraction steps simplifies the sample preparation process, taking only three minutes and eliminating the need for centrifugation by utilizing magnetic sorbent. The resulting desorption solution was directly analyzed by high-performance liquid chromatography-fluorescence detection (HPLC-FLD) without any evaporation or reconstitution steps. The integrated OPD/MSPE-HPLC-FLD method demonstrates excellent linearity (R 2 > 0.992), accuracy (relative recoveries: 85.1–109.2%), precision (RSDs≤9.7%) and detection limits (limits of detection: 0.3–2 ng/mL). It has been successfully applied to determine free BAs in various alcoholic beverages, including red wine, Baijiu, Huangjiu, and beer. This method enables rapid, sensitive and precise analysis of BAs in alcoholic beverages.
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ADVANCED FUNCTIONAL MATERIALS
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2024
·
IF 18.5
Cong Li,Ruizeng Luo,Yuan Bai,Jiajia Shao,Jianying Ji,Engui Wang,Zhe Li,Hongyu Meng,Zhou Li
Biodegradable polymers (BPs)-based triboelectric nanogenerators (TENGs) have advantages, including biocompatibility and biodegradability. However, biodegradable TENGs (BD-TENGs) possess relatively low output performance, limiting their application due to the small differences in triboelectric polarities for most BPs. Molecular doping is a simple and effective method in altering the triboelectric polarity of triboelectric materials and improving the output performance of BD-TENGs. However, there is a lack of laws and mechanism about the differences in triboelectric polarities of different doped molecules, especially of molecules with biodegradability and biocompatibility. Here, kinds of doping molecules are selected for doping in tribopositive and tribonegative materials, respectively, and their triboelectric polarities and related laws are researched. In particular, the output performance of BD-TENG is improved several times by doping small amounts of poly (propylene glycol) (PPG) and ethyl cellulose (EC), with enhanced resolution in abnormal respiratory signal monitoring. This work not only provides theoretical guidance for the selection of triboelectric molecules but also conducts in-depth research in molecular-level triboelectric mechanisms.
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JOURNAL OF CHROMATOGRAPHY A
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2024
·
IF 3.8
Yamin Liu,Xunjiang Wang,Jiaojiao Wei,Kangning Fu,Yilin Chen,Linnan Li,Zhengtao Wang,Li Yang
The severe respiratory dysfunctions associated with acute lung injury (ALI) and its sequelae have a high morbidity and mortality rate, are multifactorial, and lack a viable treatment. Considering the critical function that amino acids and derivatives play in the genesis of illnesses and the regulation of metabolic processes, monitoring the levels of metabolites associated with amino acids in biological matrices is necessary and interesting to study their pathological mechanisms. Exploring the dynamics of amino acid and derivatives levels and searching for biomarkers provides improved clinical ideas for the diagnosis and treatment of ALI. Therefore, we developed an ultra-high-performance liquid chromatography-electrospray tandem mass spectrometry (UHPLC-MS/MS) method that can simultaneously determine the amino acid and derivatives metabolic levels to study amino acid profiles in different biological samples to facilitate clinical research of ALI. In this study, 48 amino acids and derivatives, including neurotransmitters, polyamines, purines, and other types, were quantified simultaneously in a fast, high-throughput, sensitive, and reliable manner within a 15-minute run time without derivatization. No relevant studies have been reported to quantify these 48 amino acid metabolites in three biological samples simultaneously. Satisfactory linearity (R > 0.995), inter-day and intra-day accuracy (85.17–112.67% and 85.29–111.60%, respectively), inter-day and intra-day precision (RSD < 13.80% and RSD < 12.01%, respectively), matrix effects (81.00%–118.00%), recovery (85.09%–114.65%) and stability (RSD < 14.72%) were all demonstrated by the optimized method's successful validation for all analytes. In addition, the suggested method was effectively implemented in plasma, urine, and lung tissue from normal mice and mice with ALI, with the aim of finding potential biomarkers associated with ALI. Potential biomarkers were screened through multivariate statistical analysis and volcanic map analysis, and the changes of markers in ALI were again identified through heat map analysis and correlation analysis with biochemical indicators, which provided ideas and references for subsequent mechanism studies. Here, the technique created in this work offers a quick and dependable way to perform an integrated analysis of amino acids in a variety of biological materials, which can provide research ideas for understanding the physiopathological state of various diseases.
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COMPOSITES COMMUNICATIONS
·
2024
·
IF 6.5
Minghui Xu,Xiaoru Yao,Yichen Wang,Haihan Liu,Wenjia Yang,Pingping Zhang
In this paper, core-sheath nanofiber membrane (miRNA-21@DCH) co-loaded with microRNA-21 and doxycycline (DCH) was prepared by coaxial electrospinning technology for the treatment of diabetic wounds. DCH could inhibit the growth of Gram-negative and Gram-positive bacteria that easily cause infection at the wound site, thus promoting the epidermal layer thickening and dermal connective tissue hyperplasia. The cumulative release rate of DCH was 89 % after 72 h. In vitro antibacterial experiments at 24 h showed that the bacteriostatic zone diameter of miRNA-21@DCH nanofiber membrane was about 3.82 cm for Escherichia coli and 4.54 cm for Staphylococcus aureus , indicating that miRNA-21@DCH nanofiber membrane had good antibacterial effect. The microRNA-21 could promote angiogenesis and collagen deposition at the wound site, providing a favorable growth environment for tissue cell proliferation. The synergistic effect of DCH and microRNA-21 confers miRNA-21@DCH core-sheath nanofiber membrane the advantage of rapid healing of diabetic wounds. After 16 days treatment with miRNA-21@DCH nanofiber membrane, the wound basically healed and the wound surface was smooth. In addition, in vitro cytotoxicity assays showed that miRNA-21@DCH nanofiber membrane had good biocompatibility. The prepared miRNA-21@DCH nanofiber membrane provides a new and effective strategy for the synergistic treatment of diabetic wounds.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Chengtuo Niu,Yiyang Liu,Heng Li,Chunfeng Liu,Qi Li
This study aimed to elaborate the biochemical and chemosensory characteristics of doubanjiang fermentation via a two-stage controlled temperature. HL group with variable temperature (40 °C → 30 °C) and NTF group fermented following traditional technique were prepared and their volatile and non-volatile metabolites were compared through multivariate statistical analysis. HL group favored the accumulation of amino acid nitrogen, free amino acids and organic acids in the early stage and maintained adequate total acids and biogenic amines in the mid-late stage. HL group also had preferred jiang and fruity flavor through sensory evaluation. A total of 116 volatile metabolites were identified in HL or NTF groups and 22 marker volatile metabolites were screened through the combinational use of OPLS-DA and Random Forest analysis. Stronger anti-oxidant ability was observed in HL group while adequate number of acidic compounds and biogenic amines were ensured. This indicated that the two-stage controlled temperature fermentation was beneficial for doubanjiang fermentation.
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