RSC Advances
·
2021
·Vol. 11
·期 57
·35994-36006
·
IF 3.361
Jiale Cai,Xiaoting Zhong,Jiayin Liang,Can Xu,Huanzheng Yu,Minghua Xian,Chunyan Yan,Shumei Wang
Diabetes seriously endangers public health and brings a heavy economic burden to the country. Inflammation is one of the main inducing factors of type-2 diabetes (T2D) and may cause some complications of diabetes, such as diabetic encephalopathy and peripheral neuropathy. In-depth research and development of drugs to cure diabetes and complications are of great significance. Pueraria lobate is a medicinal herb used in several countries to treat many diseases. Here, two new polysaccharides (PLB-1-1 and PLB-1-2) were isolated and purified from the root of Pueraria lobata with molecular weights of 9.1 × 103 Da and 3.8 × 103 Da, respectively. The structure was evaluated by monosaccharide composition, GC-MS and NMR spectroscopy. It was determined that PLB-1-1 comprised →4)-α-D-Glcp-(1→, α-D-Glcp-(1→, →6)-β-D-Galp-(1→, →3)-α-L-Araf-(1→, →3,6)-β-D-Manp-(1→ and →4,6)-β-D-Manp-(1→, and PLB-1-2 consisted of →4)-α-D-Glcp-(1→, β-D-Glcp-(1→, →4,6)-β-D-Glcp-(1→, →3,6)-β-D-Manp-(1→ and α-L-Fucp-(1→. Furthermore, both PLB-1-1 and PLB-1-2 showed anti-inflammatory and inhibitory activities of α-glucosidase and α-amylase in vitro. Therefore, the new polysaccharides, i.e., PLB-1-1 and PLB-1-2, may be considered candidates for the treatment of diabetes and its related complications.
研究领域:
药理学
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生物化学
·
糖尿病研究
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产品关联
DOI
PubMed
高影响因子
Food Science and Technology
·
2021
·
IF 1.718
LI Tian-Xiao,LUO Cheng,GENG Zong-Ze,JIANG Zhong-Rong,JI Ling-Bo,SHENTU Hong-Qian,XIE Yun-Fei,HU Jun,LIU Yuan-Fa,LI Dong-Liang
To study the caramel reactions of disaccharides with water, the type I maltose caramel products (MCPs) and sucrose caramel products (SCPs) at three reaction stages were yielded and their reaction rates, UV absorptions, flavor compounds, and antioxidant activities were also detected. It was found that the caramel reactions of sucrose were faster than those of maltose and their reaction rates quickly increased to a high level in the middle stage. UV absorption of SCP in the late stage at 305 nm was much stronger than those of MCPs. Both the MCP and SCP obtained at 180 °C and 4 min had the highest concentrations of 5-hydroxymethylfurfural and total flavor compounds. Moreover, the total flavor concentrations of SCPs were much larger than those of MCPs and the antioxidant effects of SCPs were also stronger. Thus, a preferred condition for both two disaccharide caramel reactions was suggested at 180 °C for 4 min and the sucrose caramels could give better flavors than maltose caramels.
研究领域:
食品科学
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化学
·
营养学
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DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2025
·
IF 2.5
Huaqun Chen,Wennian Li,Chunyu Xiao,Xiaotao Hou,Kefeng Wu,Longyan Zhao,Qingxia Yuan
Pectic polysaccharides in A. marina fruit exhibit excellent prebiotic effects and may be the key to treating constipation and dysentery; however, their detailed structures still need to be clarified. In this study, we obtained two pectic polysaccharide fractions (AMFPs-3-2 and AMFPs-3-4) from this fruit for the first time and comprehensively investigated their fine structures using chemical methods, GC–MS, and 1D- and 2D-NMR. AMFPs-3-2 was identified as a type I rhamnogalacturonan (RG-I) with a backbone composed of →2)-α-Rha p -(1→4)-α-Gal p A-(1→, and the side chains mainly consisted of arabinan with non-reducing ends of α/β-Ara f . Meanwhile, AMFPs-3-4 was characterized as a homogalacturonan (HG) composed of galacturonic acid (Gal p A) and methyl-esterified Gal p A, with Gal p A, (un)saturated Gal p A and methyl-esterified (un)saturated Gal p A as non-reducing ends, and α/β-Gal p A or methyl-esterified α/β-Gal p A as reducing ends. These findings demonstrate that different types of pectic polysaccharides from A. marina fruit can be effectively separated and distinguished. The elucidation of these clear structural features provides a solid structural basis for further investigating the pharmacological activity and structure-activity relationship of polysaccharides from A. marina fruit.
研究领域:
天然产物化学
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糖生物学
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药用植物学
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分子结构解析
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多糖化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2018
·
IF 4.513
Mengjie Wu,Wei Zhou,Christian Marcus Pedersen,Hui Ma,Yan Qiao,Xiaoya Guo,Xianglin Hou,Yingxiong Wang
As counter parts of ILs , DESs have received general interest because of their comparable physicochemical properties to ILs and facile preparation from readily available chemicals. In this work, the isomeric distributions of fructose, glucose, xylose and GlcNAc in CCU, CCG and CCO , i.e. basic, neutral and acidic DES, have been determined by 13 C NMR. Higher furanose fraction of fructose was observed in CCO compared to CCG and CCU. The glucose was chosen as model compound to reveal the effect of DES on the mutarotation behavior with DMSO‑ d 6 as co-solvents. The experimental results showed that a high ratio of CCU to glucose speed up the mutarotation rate of glucose in DMSO‑ d 6 , but have little influence on the anomeric distribution. Furthermore, the mutarotation of glucose in DESs is mainly driven by the HBD , whereas HBA provides little influence. However, HBD and HBA display a synergistic effect on the glucose mutarotation process. Hydrogen bonding between hydrogen of amino in urea and oxygen of hydroxyl in glucose is the driving force for the interconversion of anomers.
研究领域:
化学
·
物理化学
·
分析化学
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产品关联
DOI
高影响因子
RENEWABLE ENERGY
·
2023
·
IF 8.7
Shao-Chao Sun,Ying Xu,Cheng-Ye Ma,Chen Zhang,Cheng Zuo,Dan Sun,Jia-Long Wen,Tong-Qi Yuan
The development of green and efficient pretreatment strategies for renewable biomass valorization is required and remains challenging. In this study, a green, efficient, and profitable pretreatment strategy using hydrothermal combined with alkaline deep eutectic solvents (HT-ADESs) was developed for the fractionation and valorization of bamboo biomass. The results demonstrated that HT pretreatment not only selectively valorized hemicellulose into functional xylooligosaccharides (XOS), accounting for 65.9% of hydrolyzed xylan , but also facilitated the subsequent delignification with ADESs. Furthermore, 29% of hemicellulose was recovered from the prehydrolyzate, revealing a branched structure of O -acetyl-4- O -methyl-glucurono- β -(1 → 4)-ᴅ-xylan. Following delignification with choline chloride/monoethanolamine containing 25 wt% peroxide, up to 98.4% glucose yield and 99.2% xylose yield were realized by enzymatic hydrolysis of pretreated residue, which were significantly higher than those of unpretreated bamboo (14.6% and 8.1%, respectively). The incorporation of an appropriate amount of water or peroxide into pure ADES facilitated lignin fractionation and reduced biorefinery costs. Overall, this work presented a promising pathway that could valorize 1 kg of dry bamboo into 490 g of fermentable sugars and 78 g of XOS, accompanied by 54 g of hemicellulose and 200 g of lignin fractions.
研究领域:
生物化学
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可再生资源工程
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Guangyu Yan,Yaru Wang,Lei Yu,Jun Bo,Hua Fang,Weizhu Chen,Yiping Zhang,Hui Chen,Zhuan Hong
Glycosaminoglycans are a class of naturally occurring macromolecular heteropolysaccharides with α-glucosidase inhibitory effects, which play an essential role in regulating postprandial hyperglycemia. However, the significant molecular weight hinders their inhibitory activity. In this study, oyster glycosaminoglycans were used for purification and depolymerization studies to obtain oyster glycosaminoglycan oligosaccharides (OGAG-Oli) and to study their inhibitory activity against α-glucosidase in vitro and hypoglycemic effect on hyperglycemic zebrafish in vivo . The results showed that the OGAG-Oli with optimal α-glucosidase inhibition obtained by the β-elimination mechanism of chondroitinase ABC had a molecular weight of about 2400 Da with five disaccharide repeating units, and their inhibitory effect on α-glucosidase was significantly enhanced with an IC 50 value of 0.12 mg/mL, which was related to the strong binding capacity with α-glucosidase as evidenced by molecular docking. FT-IR and NMR results showed that the conformation of OGAG-Oli was predominantly in the α-configuration, which indicated that its inhibitory effect on α-glucosidase mainly depended on the α-glucosidic bond therein. The enzymatic depolymerization process of glycosaminoglycans was optimized, and the process conditions were determined as phosphate buffer solution pH 8.0, reaction time 3 h, reaction temperature 30 °C, chondroitin enzyme ABC activity 0.5 mU. The hypoglycemic ability of OGAG-Oli was evaluated in diabetic zebrafish, and the results showed that OGAG-Oli at a high-dose (0.12 mg/g, bw/d) could extremely significantly reduce fasting blood glucose in zebrafish, with an effect close to that of acarbose ( p > 0.05), and exceptionally significantly ( p < 0.01) reduced the elevation of TC, TG, and LDL-C caused by prolonged hyperglycemia, and extremely significantly ( p < 0.01) increased the HDL-C level. This study showed that the structurally modified oyster glycosaminoglycans had intense α-glucosidase inhibitory activity, and exhibited biological activity of reducing glucose uptake and consequently improving body weight and lipid metabolism in diabetic zebrafish. As a bioactive ingredient of natural origin, this study provides a theoretical basis for the development and utilization of glycosaminoglycans with hypoglycemic effects in foods and drugs.
研究领域:
生物化学
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药理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Ya Li,Xingzi Wang,Jiangyan Chen,Luyun Sun,Debing Pu,Lisha Lin,Lan Luo,Xi Gong,Junxue Pu,Mingyi Wu
Diabetic foot ulcers (DFUs) as a nonhealing wound remain a clinical challenge, and the development of pro-healing and cost-effective drugs is in urgent need. Herein, we reported a novel galactosylated glycosaminoglycan (GAG) from the snail Helix lucorum , as an effective pro-healing compound. The snail GAG is composed of a heparan sulfate-like main chain and galactose side chains at C-3 of GlcNAc residue. Its main chain has a repeating disaccharide structure of → 4)-α-D-GlcNAc-(1 → 4)-α-L-IdoA 2S (1 →. This is the first example of glycosaminoglycan with galactose branches from mollusks. Pharmacological experiments showed that the H. lucorum GAG significantly promoted skin wound healing in both healthy and diabetic mice by accelerating granulation tissue regeneration, angiogenesis, and collagen deposition. The distinctive galactosylated substitution may play an important role on its pro-healing activity. Our discovery enriches the diversity of non-anticoagulant heparan sulfate-like glycosaminoglycans, and provides a potential candidate of pro-healing drug for treating diabetic wound.
研究领域:
生物化学
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药理学
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
·
2018
·Vol. 90
·期 2
·1503-1519
·
IF 0.956
JIN CUILI,YU ZHAOWEI,PENG SHUYA,FENG KE,ZHANG LIKUI,ZHOU XIAOJIAN
Exopolysaccharide (EPS) of two benthic diatoms, Amphora sp. and Stauroneis sp., with different biofilm formation abilities were investigated. The ratio of suspension-cells/biofilm-cells was employed to indicate the diatom biofilm formation abilities. The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively. The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions. Amphora sp. produced more (1.5-fold) total exopolysaccharides, but less (<0.4-fold) BF-TB-EPS than Stauroneis sp. The monosaccharides of the EPS from Amphora sp. were more diverse than those of Stauroneis sp., with 13 and 10 monomers, respectively. Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp. Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp. It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.
研究领域:
微生物学
·
海洋生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Kui Zhao,Bo Li,Dongmei He,Can Zhao,Zhengjun Shi,Binbin Dong,Duo Pan,Rahul Rangrao Patil,Zhuyun Yan,Zhanhu Guo
Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides . The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy. The structure model of hemicellulose-based polysaccharides (HBPs) was derived by combining one-dimensional and two-dimensional NMR. Monosaccharides difference and correlation were performed by partial least square analysis (PLS). Seven H-8s exhibited optimal inhibitory activities, which varied based on different sources of Danshen. The backbone structure indicated that 4-β-D-Xylp served as the main chain connected by 3-α-L-Araf or 5-α-L-Araf-1, 4-β-D-Galp, and β-D-Glcp branch, as well as α-L-Rhap, α-D-GalpA and α-D-GlcpA fragments. The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules .
研究领域:
植物化学
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天然产物化学
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药用植物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Jingwen Zhang,Binge Zhang,Feiyu Duan,Zehui Xuan,Tong Sun,Lili Lu
β-Galactosidases are important enzymatic tools for glycosylation, but their properties vary greatly with the source. Here, ten putative β-galactosidase genes, designated as bga 1 to bga 10, encoding proteins Bga1 to Bga10, were mined from an environmental metagenomic dataset comprising 119,152 sequences. Five of the encoded enzyme proteins exhibited less than 80% sequence similarity to known enzymes, but displayed conserved catalytic sites in their predicted three-dimensional models. After heterologous expression and characterization, two recombinant enzymes showed specific hydrolysis activity toward o -nitrophenyl-β- d -galactopyranoside. One of them, Bga4R, exhibited remarkable activity at pH 7.4 and 50℃, with excellent alkaline stability. Notably, Bga4R tolerated a wide range of acceptors for transglycosylation. It catalyzed galactosyl transfer to various monosaccharides and sugar alcohols, and enabling the synthesis of diverse glycosylated derivatives. This study identifies a novel GH 1 β-galactosidase as a powerful tool for glycosylation engineering, with promising potential for synthesizing galactosides valuable to food and pharmaceutical industries.
研究领域:
生物化学
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微生物学
·
合成生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Nannan Zhang,Hongkai Tian,Wenyi Zong,Qingao Fan,Jinlian Hua,Jinyi Wang,Qin Tu
The management of diabetic wounds represents a pivotal aspect of clinical care, as the hyperglycemic milieu within these wounds significantly impedes the healing process and predisposes to severe complications. Moreover, uncontrolled hemorrhage post-injury further exacerbates the impediment to wound healing. Cryogels, characterized by their porous architecture, exhibit exceptional capabilities in absorbing blood exudates, facilitating rapid hemostatic and augmenting wound healing. In this study, we utilized a sulfobetaine-modified chitosan and graphene oxide (SCG) composite as the cryogel matrix, which was subsequently impregnated with zeolitic imidazolate framework-67 (ZIF-67) to enhance the cryogel's adsorption capacity and optimizing hemostatic properties. Concurrently, glucose oxidase (GO X ) was encapsulated within ZIF-67, leveraging the framework's propensity to degrade under the acidic conditions prevalent in diabetic wounds. This controlled release of GO X in the hyperglycemic and acidic environment of diabetic wounds reduces local glucose concentrations, thereby fostering wound healing. The hemostatic efficacy of the SCG/ZIF-67/GO X cryogel was rigorously assessed through three distinct animal hemorrhage models. Furthermore, in a full-thickness skin wound model in type I diabetic mice, the SCG/ZIF-67/GO X cryogel demonstrated significant efficacy in promoting the healing of diabetic wounds. These findings highlight the SCG/ZIF-67/GO X cryogel's potential as a promising therapeutic intervention for rapid hemostasis and diabetic wounds healing.
研究领域:
生物材料学
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医学
·
药剂学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2019
·Vol. 63
·期 12
·1801008
·
IF 4.653
Haihong Chen,Qixing Nie,Jielun Hu,Xiaojun Huang,Ke Zhang,Shaoping Nie
Scope This study is aimed to investigate the kidney protective effects of glucomannans from Dendrobium officinale stem, konjac, and Aloe vera leaves on type 2 diabetic rats and explore its potential mechanisms. Methods and results Pathological and metabolomics analysis methods are adopted in this study. Compared with the model group, lower levels of fasting blood glucose, serum insulin, and glycated serum protein are observed in glucomannan-treated groups. Concentrations of serum low-density lipoprotein cholesterol, total cholesterol, triacylglycerols, and nonesterified fatty acid are significantly decreased by glucomannan treatment. Furthermore, glucomannan treatment significantly decreased the levels of uric acid, creatinine, urea in serum, and glycosuria, ketone body, and protein in the urine. Histopathological analysis showed that glucomannan treatment normalized the architecture of glomerulus. Metabolomic analysis indicated glucomannan treatment could improve urea cycle, metabolism of lipid, glucose, and amino acids on diabetes. In particular, the konjac glucomannan treatment is more effective in lipid and glucose regulation. Glucomannan from D. officinale is more effective in balancing the urea cycle and amino acid metabolism. Conclusions The disturbance of lipid, glucose, and amino acid metabolism is closely associated with the advancement of diabetic kidney disease, and glucomannan treatment could be efficient in the management of diabetic kidney disease.
研究领域:
医学
·
药理学
·
代谢生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）