COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Xiaofei Chen,Liujun Song,Xiang Jiang,Xinya Zhang
A novel morphology structure of red blood cell-like (RBC) particles with protuberances (P-RBC) was prepared through dispersion polymerization process. Based on these particles, a superhydrophobic coating film with strong water adhesion and a superhydrophilic film was fabricated depending on orientation of particles during self-assembly progress. The superhydrophobic coating film with water contact angle (CA) of 163.6° was fabricated when the direction of particle cavity was facing-down. Strong water adhesion was resulted from the larger micro-nano structure of particles. Waterdrop tightly attached to the surface of the coating film even if it was taken upside down. Whilst if the cavity of particles was upward, the coating film was transferred to be superhydrophilic of CA of 3.8° under co-efforts of storage ability of cavities, penetration capacity of channel formed by well-ordered particles, and increased surface roughness . This work gives that anisotropy colloidal particles have potential application with their superior wetting characteristics in many fields, such as material transfer process, waterproof protection and oil-water separation.
研究领域:
材料科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Chunru Wang,Qingyang Lu,Yingjie Xiang,Yulan Yin,Junyao Li,Yalu Liu,Xiaochen Wu
Caffeic acid (CA) is an antioxidant phenolic compound that enriched in coffee beans, however, its administration often restrains by the instability and low solubility. Nanoparticle encapsulation is an effective approach to improve the therapeutic activity of CA . For example, silk sericin (SS), a natural biomaterial finds applications in food, cosmetics and biomedical fields, is proved here to be an appropriate encapsulation agent for CA, and a SS/CA composite nanoparticle has been fabricated. To further improve the biocompatibility of SS/CA, a red blood cell membranes (RM) cloaking strategy is adopted. The as-formed SS/CA/RM preserves the antioxidant activity of CA, and shows satisfactory biocompatibility especially under high concentration. Hope this can provide a potential appropriative strategy to adjust the chemical stability of insoluble drugs and to improve their biocompatibility.
研究领域:
生物医学工程
·
药剂学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
Drug Delivery and Translational Research
·
2020
·Vol. 10
·期 5
·1278-1287
·
IF 2.664
Chu Yuqi,Zhang Jinfeng,Pan Hao,Shi Jinyan,Wang Jinglei,Chen Lijiang
Recently, biomimetic hybrid drug delivery systems, especially erythrocyte membrane-based drug delivery systems, have been utilized to achieve high bioavailability, and biocompatibility, in the meantime, to reduce immunogenicity and effectively evade phagocytosis of the host immune system. Here, we developed a novel drug delivery system of red blood cell membrane-derived vesicles (RDVs) cloaked poly (acrylic acid)-cystamine hydrochloride-D-α-tocopherol succinate (PAAssVES) nanoparticles. The PAAssVES nanoparticles were prepared via emulsification and solvent volatilization method, followed by loading of the model anti-cancer drug, sorafenib (SFN). Then RDVs and SFN-PAAssVES nanoparticles were uniformly mixed and co-extruded through polycarbonate membrane. The prepared RDV-coated nanoparticles (RDV-NPs) had good stability, with a zeta potential of − 10.7 mV and particle size of 113.5 nm. MTT assay was used to analyze the effects of RDV-NPs on cell viability in two kinds of gastric cancer cell lines BGC-823 and MKN-45. The results showed that RDV-NPs significantly decreased cell viability. In vitro drug release investigation showed that RDV-NPs had good sustained release properties and the cumulative release was 71.5% in 72 h. In pharmacokinetic studies, SD male rats’ intravenous injection with RDV-NP solution showed a more smooth plasma concentration-time profile. Compared with free SFN treatment and SFN-PAAssVES group, RDV-NPs enhanced the AUC by about 4.1-fold and 2.0-fold. The MRT and t 1/2 of RDV-NPs were increased to 23.670 ± 2.347 h and 24.450 ± 2.652 h. Our study demonstrated the promise of using RDV-NPs as a long circulating anti-cancer drug delivery system.
Graphical abstract
研究领域:
药学
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肿瘤学
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生物材料学
✓ 已验证
DOI
高影响因子
Nutrients
·
2022
·Vol. 14
·期 15
·3095
·
IF 6.706
Zhi Huang,Ping Guo,Ying Wang,Ziming Li,Xiaochen Yin,Ming Chen,Yong Liu,Yuming Hu,Bo Chen
Objective: The present study aims to measure docosahexaenoic acid (DHA) in both the plasma and erythrocyte of a child population and compares them with respect to their associations with dietary and metabolic risk patterns. Methods: A cross-sectional study was conducted, and a total of 435 children ages 5–7 years old were recruited. Diet information was collected using a food frequency questionnaire (FFQ). The physical indicators, blood pressure, and glycolipid metabolic indicators were determined. The plasma and erythrocyte DHA were analyzed using a gas chromatography mass spectrometer. Principal component analysis was used to identify dietary and metabolic risk patterns. Multivariate regression analyses were used to investigate the associations of DHA status with dietary and metabolic risk patterns. Results: A significant correlation between plasma and the erythrocyte DHA concentration was found (r = 0.232,p< 0.001). A diversified dietary pattern characterized that a high intake of diversified foods had a positive association with the plasma DHA level (β = 0.145, 95% CI: 0.045~0.244,p= 0.004). Children of obesity risk patterns with a high weight, pelvis breadth, BMI, upper arm circumference, and chest circumference had lower plasma DHA concentrations (OR = 0.873, 95% CI: 0.786~0.969,p= 0.011). Children with higher plasma and erythrocyte DHA concentrations were adhered to blood lipid risk patterns with high CHOL and LDL-C levels. The plasma DHA (OR = 1.271, 95% CI: 1.142~1.415,p< 0.001) had a stronger association with a blood lipid risk pattern than erythrocyte (OR = 1.043, 95% CI: 1.002~1.086,p= 0.040). Conclusions: The diversified dietary pattern had a higher plasma DHA concentration. Lower levels of plasma DHA were positively associated with obesity in children. DHA in plasma appears to be more strongly associated with blood lipid metabolism than erythrocyte. Plasma DHA may be a more sensitive bidirectional biomarker to evaluate the recently comprehensive diet intake and metabolic risk of children.Keywords:docosahexaenoic acid;plasma;erythrocyte;dietary patterns;metabolic risk patterns
研究领域:
营养学
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儿科医学
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代谢研究
✓ 已验证
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2019
·
IF 4.018
Jinjin Guo,Xiaoyu Wang,Shuxian Zhu,Lu Liu,Xiaofeng Jiang,Dongdong Ban,Lidong Li
We combined the unique natural oxygen transporting function of red blood cells (RBCs) with photosensitive nanocomposites to create biomaterials with strong singlet oxygen ( 1 O 2 ) generation. During cold storage for a long time, structural changes to RBCs may occur, which reduce their oxygen carrying capacity. The nanocomposite developed herein featured dual emission characteristic, which enabled evaluation of the status of the RBCs. On the basis of energy transfer from the conjugated polymer to iridium(III) complexes, the formed nanocomposites show intense, long-lived and oxygen-sensitive emission. When modified with 1,2-distearoyl- sn -glycero-3-phosphoethanolamine- N -[methoxy(polyethyleneglycol)-2000], the nanocomposites non-invasively entered RBCs through hydrophobic interactions . We measured the quenching effect of oxygen on the emission of the iridium(III) complexes. The differential fluorescent signals and fluorescence lifetime images indicated the storage history of the RBCs. RBCs in good condition stored oxygen. When engineered with nanocomposites, RBCs generated large amounts of cytotoxic 1 O 2 upon irradiation. These decorated RBCs might serve as a new kind of photosensitive biomaterial.
研究领域:
生物材料学
·
医学工程
·
光动力治疗
✓ 已验证
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2025
·
IF 4.5
Hongyu Zhong,Yichen Li,Faisal Raza,Jiyuan Xie,Ruonan Rong,Mingfeng Qiu,Jing Su
Colorectal cancer (CRC) is a malignant epithelial tumor with high morbidity and mortality. In CRC treatment, irinotecan (CPT-11) as a chemotherapeutic drug is widely applied. However, its half-life is short, leading to large dosages and severe side effects. Red blood cells (RBCs) are biocompatible drug carriers with high capacity, avoiding premature drug degradation and achieving slow drug release. Nanoalumina (AN) is an emerging immune adjuvant that can enhance the immune response. Here, we used RBCs as carriers and absorbed AN to construct AN–CPT-11–RBCs. CPT-11 would induce tumor cell death, releasing much tumor antigen, while AN would activate immune cells to recognize newly released antigens and induce lymphocyte proliferation, enhancing the antitumor effect simultaneously. With loading amounts of 4 mg of CPT-11 and 3 mg of AN per 109 RBCs, AN–CPT-11–RBCs had similar properties to natural RBCs. In vivo, AN–CPT-11–RBCs could circulate for 9 days and stimulate the proliferation of lymphocytes in the spleen and tumor tissue, having a higher tumor growth inhibition rate of 74.01% and a lower frequency of administration. In conclusion, AN–CPT-11–RBCs attain the co-delivery of CPT-11 and AN for the synergistic treatment of CRC.
研究领域:
肿瘤学
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药理学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2025
·
IF 4.8
Sha Li,Si-Ming Yan,Li-Wei Zhang,Xiao-Yan Yang,Zhong Guo
Introduction: Polydopamine nanoparticles (PDA NPs) exhibit numerous outstanding characteristics, including simple preparation, broad light absorption, drug binding ability, excellent biocompatibility and adhesive properties, making them suitable for biomedical application. However, the limited information on their hemocompatibility may hinder their progression from laboratory research to clinical application.Methods: In this study, we investigated comprehensively the hemocompatibility of PDA NPs, assessed the effects of PDA NPs on red blood cells (RBCs) morphology and lysis, fibrinogen structure and conformation, blood coagulation, platelet activation, complement system activation, and organ toxicity.Results: The results indicated that PDA NPs can induce morphological changes and hemolysis in RBCs in a concentration-dependent manner. Interactions with fibrinogen suggested a disturbance in the protein’s microenvironment without significantly altering its secondary structure. This study also revealed that PDA NPs have a concentration-dependent effect on blood coagulation, platelet activation, and complement system activation. Additionally, PDA NPs showed no significant acute toxicity after intravenous injection.Conclusion: The findings offer important insights into the hemocompatibility of PDA NPs, which is essential for their safe and effective clinical use. Understanding their interactions with blood components is key to ensuring their compatibility in biomedical applications. These results are vital for guiding the development of PDA NPs for medical use, particularly in blood-contacting applications.
研究领域:
生物医学工程
·
材料科学
·
血液学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Xuejie Xie,Zhenyu Hou,Guangxin Duan,Shitong Zhang,Hong Zhou,Zaixing Yang,Ruhong Zhou
Boron nitride (BN) nanosheets have emerged as promising nanomaterials in a wide range of biomedical applications. Despite extensive studies on these bio-nano interfacial systems, the underlying molecular mechanisms remain elusive. In this study, we used hemolysis assays and morphology observations to demonstrate for the first time that BN nanosheets can cause damages to the red-blood-cell membranes, leading to significant hemolysis. Further molecular dynamics simulations revealed that BN nanosheets can penetrate into the cell membrane and also extract considerable amount of phospholipid molecules directly from the lipid bilayer. The potential of mean force calculations then showed that their penetration effect was thermodynamically favorable due to the strong attractive van der Waals interactions between BN nanosheets and phospholipids. Overall, these findings provided valuable insights into the interaction of BN nanosheets with cell membranes at the atomic level, which can help future de novo design of BN-based nanodevices with better biocompatibility.
研究领域:
生物医学
·
纳米科学
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2022
·
IF 11.467
Mingming Song,Shuqi Dong,Xiaofei An,Wenxiang Zhang,Ning Shen,Yanbo Li,Caixia Guo,Chang Liu,Xiao Li,Siyu Chen
Chemotherapy is a difficult treatment for cancer patients because of the low effective accumulation of chemo-drugs and their detrimental side effects. Nanoparticles have shown promise as a solution to these problems. However, the known differences in the porosity and vascularization of tumor vessels, and other factors, including the potential formation of a “protein crown,” the short half-life time in circulation, and the low drug distribution, often limit their application. To address these problems, biomimetic nanoparticles coated with cell membranes have been developed and shown to have advantages such as prolonged circulation, high biocompatibility, and enhanced targeting abilities in drugs and nanoparticles, thus exhibiting good application prospects in cancer therapy for liver, lung, and melanoma cancers. Accordingly, we designed a PH-sensitive biomimetic nanodrug delivery system with a delicate “core–shell” structure based on red blood cell membranes. Briefly, core nanoparticles were synthesized by the self-assembly of natural amphoteric polymers, including hydrophilic carboxymethylcellulose sodium and hydrophobic stearic acid . For the shell structure, red blood cell membranes were modified using folic acid by a lipid tether (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) to increase tumor-targeting ability, whereas polyethylene glycol was inserted to decrease lipid tether modification-induced potential sequestration by either the mononuclear phagocyte system or the reticuloendothelial system. Via a series of formulation optimizations, paclitaxel was packaged into the red blood membrane-based core–shell nanoparticles with an average size of 226.9 ± 2.75 nm and a negative Zeta potential of −14.5 ± 0.3 mV. More importantly, the examinations focusing on CD47, a representative red blood cell membrane protein, revealed not only the successful establishment of the membrane shell but also the right-side-out membrane orientation on our core–shell nanoparticles. Our nanodrug delivery system showed good biocompatibility and sensitivity to acidic tumor microenvironments while effectively prolonging the circulation time of paclitaxel and further enhancing its antitumor effects on epithelial malignancies, including liver, lung, and melanoma cancers. In particular, our nanodrug delivery system significantly alleviated paclitaxel-induced renal toxicity . Taken together, our findings highlight that the red blood membrane-based core–shell nanoparticle is a promising biomimetic nanodrug delivery system for functionally delivering chemotherapeutic drugs, and it has promise in clinical applications.
研究领域:
药学
·
纳米技术
·
肿瘤学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Regeneration
·
2023
·Vol. 12
·期 1
·1-18
·
IF 0
Liu WenYe,Lin ShuHui,Li LingYa,Tai ZhiPeng,Liu Jing-Xia
EAF1 and EAF2, the eleven-nineteen lysine-rich leukemia (ELL)-associated factors which can assemble to the super elongation complex (AFF1/4, AF9/ENL, ELL, and P-TEFb), are reported to participate in RNA polymerase II to actively regulate a variety of biological processes, including leukemia and embryogenesis, but whether and how EAF1/2 function in hematopoietic system related hypoxia tolerance during embryogenesis remains unclear. Here, we unveiled that deletion of EAF1/2 (eaf1−/− and eaf2−/−) caused reduction in hypoxia tolerance in zebrafish, leading to reduced erythropoiesis during hematopoietic processes. Meanwhile, eaf1−/− and eaf2−/− mutants showed significant reduction in the expression of key transcriptional regulators scl, lmo2, and gata1a in erythropoiesis at both 24 h post fertilization (hpf) and 72 hpf, with gata1a downregulated while scl and lmo2 upregulated at 14 hpf. Mechanistically, eaf1−/− and eaf2−/− mutants exhibited significant changes in the expression of epigenetic modified histones, with a significant increase in the binding enrichment of modified histone H3K27me3 in gata1a promoter rather than scl and lmo2 promoters. Additionally, eaf1−/− and eaf2−/− mutants exhibited a dynamic expression of canonical WNT/β-catenin signaling during erythropoiesis, with significant reduction in p-β-Catenin level and in the binding enrichment of both scl and lmo2 promoters with the WNT transcriptional factor TCF4 at 24 hpf. These findings demonstrate an important role of Eaf1/2 in erythropoiesis in zebrafish and may have shed some light on regeneration medicine for anemia and related diseases and on molecular basis for fish economic or productive traits, such as growth, disease resistance, hypoxia tolerance, and so on.
研究领域:
发育生物学
·
血液学
·
分子生物学
✓ 已验证
DOI
Laser & Photonics Reviews
·
2024
·
IF 9.8
Mengxuan Niu,Yijin Wang,Liting Duan,Rui Sun,Renjie Zhou
The Compact Morpho-Molecular Microscopy (CM 3 ) is proposed and demonstrated for multi-functional imaging and characterization of living cells and material structures by simultaneously offering quantitative phase imaging (QPI), dispersion characterization, and fluorescence imaging. The compactness and stability of CM 3 are realized by propagating lasers of different wavelengths in specially treated optical fibers and fiber-based beam splitters and wavelength division multiplexers, as well as simplifying the detection scheme through Fourier-space multiplexing and implementing a single camera for both QPI and fluorescence imaging. Quantitative phase maps of two wavelengths are retrieved from a multiplexed interferogram, and a synthesized wavelength phase map is derived to guide the height profiling of samples with extended depth. With the wavelength resolved phase maps, a physical model is derived to obtain sample dispersion parameters, which further enables us to quantify the hemoglobin concentration of red blood cells in real time. By inserting an appropriate emission color filter, fluorescence imaging is realized using the same camera, which significantly broadens the cell imaging applications of CM 3 . As a cost-effective and multifaceted imaging method, CM 3 may find many promising applications in live-cell imaging and material characterization.
CAS / MDL:
研究领域:
生物医学工程
·
显微成像
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2016
·Vol. 27
·期 8
·085106
·
IF 3.573
Lang Rao,Jun-Hua Xu,Bo Cai,Huiqin Liu,Ming Li,Yan Jia,Liang Xiao,Shi-Shang Guo,Wei Liu,Xing-Zhong Zhao
Suppression of the reticuloendothelial system (RES) uptake is one of the most challenging tasks in nanomedicine. Coating stratagems using polymers, such as poly(ethylene glycol) (PEG), have led to great success in this respect. Nevertheless, recent observations of immunological response toward these synthetic polymers have triggered a search for better alternatives. In this work, natural red blood cell (RBC) membranes are camouflaged on the surface of Fe3O4 nanoparticles for reducing the RES uptake. In vitro macrophage uptake, in vivo biodistribution and pharmacokinetic studies demonstrate that the RBC membrane is a superior alternative to the current gold standard PEG for nanoparticle 'stealth'. Furthermore, we systematically investigate the in vivo potential toxicity of RBC membrane-coated nanoparticles by blood biochemistry, whole blood panel examination and histology analysis based on animal models. The combination of synthetic nanoparticles and natural cell membranes embodies a novel and biomimetic nanomaterial design strategy and presents a compelling property of functional materials for a broad range of biomedical applications.
研究领域:
纳米医学
·
生物材料
·
药代动力学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）