JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Qing Ning,Dali Wang,Jiahui An,Qi Ding,Zhiyi Huang,Yue Zou,Fan Wu,Jing You
Toxicological effects of nanoplastics have been demonstrated in a variety of organisms, yet their impacts on bacteria, especially on the antibiotic resistance evolution remain under explored. Herein, we report individual and combined effects of nano-polystyrene (nano-PS) and erythromycin (ERY) on growth and resistance mutations of Escherichia coli . The toxicity of nano-PS was dependent on size and functional modifications, with 30 nm and amino-modified PS (PS-NH 2 , 200 nm) showing the greatest toxicity. Adsorption of nano-PS onto bacterial surface and the subsequent increase of intracellular ROS or the probable mechanical damage were considered as the primary toxic mechanisms. Furthermore, nano-PS increased the bacterial resistance mutations, which was due to the oxidative damage to DNA and the SOS response. In addition, PS-NH 2 presented synergistic effects with ERY while non-modified PS had no impact, although both of them showed adsorption capacity to ERY. This was likely because the positively charged PS-NH 2 acted as a carrier of ERY and enhanced the interactions between ERY and the bacteria. Our findings raised the concerns about the risk of nanoplastics in accelerating the bacterial resistance evolution, and highlighted the necessity of including combined effects of nanoplastics and co-contaminants in risk assessment.
研究领域:
环境科学
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2021
·
IF 3.498
Can Tao,Fang Zhao,Zi-Wei Tang,Luxi Zhang,Qiang Niu,Gao Cao,Li-Min Zhao,Wenhuan Huang,Ping Zhao
Magnetic mesoporous ZrO 2 (Fe 3 O 4 @ZrO 2 , MMZr) nanoparticles blocked by bismuth oxide (Bi 2 O 3 ) are presented and efficiently deliver chemotherapeutic agent daunomycin (DNM) to the targeted tumor cells. Bi 2 O 3 not only rapidly dissolves in the acidic condition of cancer cells to release the loaded chemotherapeutic drug but also enhances the anti-cancer effect through synergistic effects of the chemotherapy and Bi 3+ ions. By monitoring the treatment process, compared with the free drug DNM, the drug loaded DDS exhibit more significant suppression against cancerous cells growth such as A549 and MCF-7 cancer cells and lower cytotoxicity against normal Hacat cells. Mechanism research indicates that the drug loaded DDS can arrest the cell cycle at G0/G1 phase, change the mitochondrial membrane potential and finally induced the apoptosis of the cancerous cells. These results indicate that the Bi 2 O 3 -gated ZrO 2 platform is a promising approach to serve as a pH-sensitive DDS in the treatment of various cancers by chemo/ionic synergistic effects.
研究领域:
纳米技术
·
癌症治疗
·
药物递送系统
·
材料科学
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Yanxiang Zhang,Huiling Liu,Xiaohu Dai,Jing Wang,Yunpeng Shen,Peng Wang
Erythromycin fermentation residue (EFR) is one kind of biological waste with high organic matter content. The recycling of EFR is not only beneficial to the environmental protection, but also to the economic development. In this study, the release of organic matter, nitrogen (N), phosphorus (P) and heavy metals (HMs) from EFR under heat-activated persulfate (PS) oxidation conditioning was investigated. Results indicated that oxidation conditioning promoted the release of soluble chemical oxygen demand (SCOD). Heat-activated PS oxidation conditioning boosted the release of total nitrogen (TN), ammonia‑nitrogen (NH 4 + -N) and nitrate‑nitrogen (NO 3 − -N) into the supernatant, as well as the decomposition of organic nitrogen (ON). Concurrently, heat-activated PS oxidation conditioning facilitated the release of total phosphorus (TP), orthophosphate (PO 4 3− -P) and organic phosphorus (OP) into the supernatant, and the decomposition of OP. Furthermore, heat-activated PS oxidation conditioning resulted in the increase of release efficiencies of HMs. Therefore, heat-activated PS oxidation conditioning was beneficial to the release of organic matter, nutrients and HMs.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2017
·Vol. 13
·期 12
·1647-1659
·
IF 4.521
Cao Jun,Zheng Haoran,Hu Rui,Liao Jianhong,Fei Zengming,Wei Xuan,Xiong Xiong,Zhang Fanglin,Zheng Hua,Li Dan
The drug controlled release responding to tumour microenvironment is a hotspot for cancer therapy research. Herein, pH-sensitive shell-core nanoparticles (NPs) were devised and fabricated for tumour treatment. The detailed characterisations of their structures demonstrated the successful
formation of the precursors and NPs. More importantly, the NPs show excellent features, i.e., negative surface potential, narrow size distribution, and suitable dimension for cell penetration. And they have superior stability in pH 7.4 buffer (i.e., blood environment). In addition, owing to
the existence of the pH-labile imine bond, a controlled release of drugs from NPs was achieved in drug release test. Confocal laser scanning microscopy (CLSM) and flow cytometry proved the internalization of NPs into HeLa cells and their further translocation into cell nuclei. The cytotoxicity
results also revealed a remarkable cytotoxicity of the NPs to HeLa cells. Thus, this type of NP may be potentially useful for the efficient drug delivery.
研究领域:
药剂学
·
肿瘤学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yi He,Xiaoyu Yang,Shunbi Xie,Runzi Zhang,Yao Liu,Mengjun Wang
Roxithromycin (ROX) is an effective antibiotic, but its prolonged administration may lead to hepatotoxicity and other side effects. To address this concern, the present study introduced a novel strategy that integrated a CHA-mediated dual-end DNA walker with hydrogen-bonded organic frameworks (HOFs) combined with Au nanoparticles (NPs) as efficient SERS substrates, enabling highly sensitive detection of ROX in food. Notably, HOF possessed a large specific surface area and strong adsorption properties that making it excellent platforms for the in-situ synthesis of abundant Au NPs and contributed to robust and uniform SERS signals. When ROX was present, it can trigger the activation of CHA and resulting the formation of a dual-end DNAzyme structure. Meanwhile, under the catalytic action of Mg 2+ , DNAzyme initiated the DNA walker mechanism and generated a substantial amount of Output DNA. Moreover, the Au@4-NTP@Ag core-shell structure generated numerous SERS “hot spots” and combined with the signal-amplifying capability of HOF@Au, leading to a remarkable improvement in SERS sensitivity. Based on this mechanism, the developed sensor employs a “signal-on” strategy for ultra-sensitive detection of ROX, achieving a detection range from 1.0 × 10 −6 to 1.0 × 10 −13 mol/L with a detection limit of 3.72 × 10 −14 mol/L. Furthermore, the method had been successfully applied to the detection of ROX in real food samples, including milk and honey, offering a promising and innovative framework for the development of next-generation SERS biosensors. This approach demonstrated significant potential for highly selective and sensitive antibiotic monitoring in food safety applications.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
食品安全
·
抗生素检测
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Bing-Jie Ni,Shuting Zeng,Wei Wei,Xiaohu Dai,Jing Sun
The macrolide antibiotic roxithromycin is widely detected in varying aquatic environments, especially in the wastewater systems, as an emerging contaminant and leads to significant impacts on the microorganisms involved. In this study, the impact of a shock load of roxithromycin on waste activated sludge (WAS) anaerobic digestion was comprehensively investigated. The biochemical methane potential tests showed that the methane production from WAS anaerobic digestion was significantly inhibited by roxithromycin. With the dosage of roxithromycin increasing from 0 to 1000 μg/L, the maximum cumulative methane production decreased from 163.5 ± 2.6 mL/g VS to 150.9 ± 4.5 mL/g VS. In particular, roxithromycin inhibited the acidogenesis and methanogenesis in WAS anaerobic digestion, leading to the decreased methane production. The methanogenic archaea in the studied system mainly belonged to the genera of Methanoseata, Candidatus Methanofastidiosum and Methanolinea and their relative abundances also decreased with roxithromycin addition. The analysis of antibiotic resistance genes (ARGs) in the digested sludge indicated that the abundances of most ARGs detected in this study were increased with roxithromycin exposure, suggesting the potential of growing antibiotic resistance, which was probably caused by enhancing the effect of esterases, methylases and phosphorylases. This work reveals how roxithromycin affects the WAS anaerobic digestion and the change of ARGs in the anaerobic digestion with roxithromycin exposure, and provides useful information for practical operation.
研究领域:
环境工程
·
微生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 27
·18651-18657
·
IF 3.9
Jie Yu,Danwei Cen,Yongcheng Chen,Hailan Zhao,Mengyue Xu,Sulan Wu,Shuo Wang,Qiao Jin,Ting Shen
Antibiotic resistance is a big threat to public health. How to improve the therapeutic efficacy of conventional antibiotics is an effective way to address this issue. In order to enhance the antibacterial activity of conventional antibiotic erythromycin (EM), EM is conjugated to positively charged ε-poly-L-lysine (EPL) to obtain EPL modified EM (EPL-EM). The grafting ratio of EM can be calculated from the 1H NMR spectrum. EPL-EM is stable in physiological environment, while EM can be readily released from EPL-EM upon incubating with esterase which can be secreted by most bacteria. Because of the presence of cationic EPL, EPL-EM showed much stronger antibacterial activity than free EM, with much lower minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Moreover, compared to free EM, the development of drug resistance can be slowed down if EPL-EM is used, which can be ascribed to the reduction of EM dosage. Meanwhile, EPL-EM cannot induce hemolysis and cytotoxicity, which indicates that EPL-EM exhibits excellent biocompatibility. The design of EPL-EM with enhanced antibacterial activity and excellent biocompatibility provides an innovative way to combat antibiotic resistance.
研究领域:
药理学
·
微生物学
·
治疗学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2023
·
IF 2
Xiaoyu Zhang,Peiran Meng,Wei Wang,Xiwen Li,Zuan Yang,Shaokai Du,Yue Sun
Simultaneous determination of erythromycin (Ery) and hemoglobin (Hb) is of great significance for clinical diagnosis of many diseases. ZnO nanorods modified with Au nanoparticles were fabricated as an interface on the flexible carbon cloth (CC) and utilized to simultaneously determine Ery and Hb by a simple electrochemical method. The results demonstrated that Au/ZnO/CC displayed excellent analytical performance for Ery with a low detection limit of 3.38 × 10−7 mg/L and a linear range from 1 × 10−6 to 1 × 10−1 mg/L. At the same time, the sensor also showed a sensitive response to Hb with a low detection limit of 2.07 × 10−7 mg/L and a linear range from 1 × 10−6 to 1 × 10−1 mg/L. This work provides a viable approach for designing a flexible electrode and sensitive simultaneous determination of Ery and Hb.
研究领域:
分析化学
·
纳米技术
·
电化学
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
DENTAL MATERIALS
·
2022
·
IF 5.687
Ludan Qin,Shuo Yao,Wenqing Meng,Jiajia Zhang,Ruirui Shi,Chuanjian Zhou,Junling Wu
Objectives Secondary caries is the primary issue that causes restoration failure. The objectives of this study were to: (1) synthesize silanized hydroxyapatite nanofibers loaded with erythromycin ( [email protected] ); (2) evaluate the mechanical property, antibacterial activity, and remineralization capability of the novel dental resin containing [email protected] Methods s-HAFs were prepared by the solvothermal approach and loaded with EM. Characterization and antibacterial activity were evaluated. Subsequently, [email protected] were incorporated into dental resin at different mass fractions (5 %, 10 %, 15 %, and 20 %), and then they were submitted to characterization, including mechanical property, antibacterial activity, remineralization capability, and cytotoxicity. Results [email protected] were successfully synthesized, and they exhibited excellent antibacterial activity. Resin containing 15 % [email protected] exhibited the best flexural strength (118.67 ± 15.71 MPa) and elastic modulus (2.02 ± 0.30 GPa) (P < 0.05), which were increased by 65.43 % and 90.7 %, compared to those of neat resin, respectively. Resin with 15–20 % [email protected] showed high antibacterial rate (>85 %) when compared control group (P < 0.05). Furthermore, resin also exhibited a definite remineralization capability and good biosafety in vitro . Significance This novel multifunctional resin with improved mechanical property, desirable antibacterial activity and remineralization capability is promising to combat secondary caries.
研究领域:
牙科材料
·
纳米技术
·
生物材料
·
材料科学
·
牙科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Lei Cao,Yuanling Li,Peining Li,Xueting Zhang,Lei Ni,Li Qi,Haitao Wen,Xinbo Zhang,Yufeng Zhang
The present study operated the novel moving bed biofilm reactor-nanofiltration-membrane bioreactor (MBBR-NF-MBR) with loose polyamide NF membranes for the first time to treat roxithromycin (ROX) wastewater. Results showed that both MBBR-NF-MBRs achieved superior COD removal of 98.4% and 97.2% and excellent removal of ROX at 74.1% and 65.5%, respectively. The main membrane fouling mechanism was reversible fouling caused by the combination of abundant polysaccharides, proteins and Ca-P precipitates, which could be effectively removed by acidic cleaning. Sorption and biodegradation were the main removal routes of ROX in MBBR . Partial retention of loose NF membrane contributed to microbial metabolism and increased microbial diversity, especially the genera Hyphomicrobium in attached biofilm, which was reasonable for ROX removal. The cleavage of cladinose, demethylation, phosphorylation and β-oxidation in macrolactone ring were the main biotransformation reactions of ROX. This study provides novel insights for micropollutants wastewater treatment by using loose NF membrane in MBR.
研究领域:
环境工程
·
废水处理
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2019
·
IF 4.175
Fen-Ying Kong,Lei Yao,Rong-Fang Li,Heng-Ye Li,Zhong-Xia Wang,Wei-Xin Lv,Wei Wang
Nitrogen-doped reduced graphene oxide loading with Au-Ag bimetallic nanoparticles (NG-Au-Ag) was synthesized by a facile two-step strategy. The successful formation of NG-Au-Ag nanocomposites were confirmed by transmission electron microscopy (TEM), Raman spectroscopy , X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) analysis. Furthermore, NG-Au-Ag nanocomposites were modified onto the electrode surface for the voltammetric detection of daunorubicin (DNR). The results showed that the NG-Au-Ag modified electrode expressed high electrocatalytic activity toward the redox of DNR, allowed detection of DNR with wide linear range (0.01 μg mL −1 to 15 μg mL −1 ) and low detection limit (3 ng mL −1 ). The modified electrode also exhibited satisfactory performance for the detection of DNR in human serum samples.
研究领域:
材料科学
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 48
·23039-23049
·
IF 3.925
Yongchang Ouyang,Miao Liang,Fangzhen Wang,Songqing Mei,Guangquan Mo
Herein, supported by the attractive structure of zeolitic imidazolate frameworks (ZIFs), novel porous thorny carbon dodecahedron implanted with hollow cobalt oxide nanospheres (PCD-HC) was prepared by the two-step pyrolysis of ZIFs. Morphology and structure investigations showed that PCD-HC was a highly porous rhombic dodecahedron embedded with hollow nanospheres. Elemental mapping revealed that the main dodecahedral structure of PCD-HC was a carbonized framework doped with N species. Besides, well-defined hollow Co3O4 nanospheres with abundant O vacancies were implanted. Due to the highly porous structure, embedded active species, and graphite-like structure, PCD-HC displayed distinguished electrocatalysis towards doxorubicin (DOX) with a pair of highly symmetric redox peaks and fast direct electron transfer of anthracycline. The direct electrochemistry and catalytic mechanism of the DOX redox reaction at PCD-HC were elucidated, and a PCD-HC based electrochemical sensor was developed for the DOX sensing with a broad linear range from 1.0 nM to 1.0 μM. The limit of detection was obtained as 0.15 nM (S/N = 3). Besides, PCD-HC sensor also displayed a wide detection range at a high concentration from 2.0 to 12.0 μM. Moreover, PCD-HC sensor presented a high selectivity with strong anti-interference capability towards common inorganic ions and small biomolecules. The reproducibility, repeatability, and stability were examined with satisfactory results. Significantly, a PCD-HC sensor was applied in the monitoring of DOX in HeLa cells inhibiting process, indicating a substantial detection platform for DOX sensing.
研究领域:
材料科学
·
分析化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）