Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 32
·5290-5302
·
IF 4.776
Linawati Sutrisno,Sixiang Wang,Menghuan Li,Zhong Luo,Chunli Wang,Tingting Shen,Peixing Chen,Li Yang,Yan Hu,Kaiyong Cai
Bacterial biofilm formation and oxidative stress induced by the production of reactive oxygen species (ROS) are major causes of implant failure. An emerging approach to overcome these issues is to combine chitosan–polycaprolactone (PCL) nanofibers and polyelectrolyte multilayers composed of tannic acid (TA) and gentamicin sulfate (GS) for the localized co-delivery of antioxidants and antibiotics from the titanium surface. The integration of nanofibers (NFs) and layer-by-layer (LBL) technology could provide a larger surface area and thus increase the number of cationic sites of Ti substrates. The coating of NF substrates with TA/GS resulted in higher (p < 0.05 or p < 0.01) cellular activities than those of Ti substrates, including enhanced proliferation and gene expression. Furthermore, in vitro investigation demonstrated that TA/GS-incorporated Ti-polydopamine (PDA)/NF implants exhibited excellent stability, and antibacterial and antioxidant properties. The results showed that Ti-PDA/NF/LBL substrates have a biodegradable character in vivo. All the results indicated that the combination of NFs and the bacteria-triggered antibiotic-releasing coating could be used for the tailored co-delivery of antibacterial and antioxidant agents from various metallic implantable devices to effectively improve early bone healing even under ROS stress and decrease the risk of biofilm-associated infections in patients.
研究领域:
生物材料
·
生物医学工程
·
材料科学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Materials Today
·
2022
·
IF 8.663
Yanlin Chen,Gang Zheng,Yujie Bian,Haiwei Ma,Chenqiang Jin,Zhichao Hu,Haohan Su,Xinli Hu,Zhiyong Qian,Liyan Shen,Wenfei Ni
The instant and long-term stabilities of the implanted cage between the two vertebras are key factors for successful spinal fusion surgery. Instead of simply induce bone formation by including osteo-inductive cytokines carrying materials in the cavum of the cage, the modification of the cage surfaces to facilitate cage-bone integration yet promoting bone regeneration is becoming more essential. In this study, we used the titanium mesh scaffold (TMS) as the model cage and activated the surface with the synergism of a bone-conductive polyelectrolyte multilayer film (PEM) and the bone-inductive drug psoralen (PSO). The Hyaluronic Acid/Polymerizated Dopamine modified Hydroxyapatite/Chitosan (HA/PDHA/CHI) multilayer film was constructed on the scaffold in layer-by-layer (LBL) fashion, and PSO was loaded on the scaffold via post-diffusion. SEM and FTIR confirmed the successful coating of HA/PDHA/CHI multilayer film on TMS as well as the effective loading of psoralen. The rough and porous surface structure of the hybrid PEM facilitated the proliferation and adhesion of rat bone mesenchymal stem cells (rBMSCs), and the PEM coated scaffolds exhibited advantages in PSO loading and retention. In vitro experiments demonstrated that psoralen had direct and effective osteogenic ability and the ability was closely related to the activation of the autophagy, while PEM promotes new bone formation by favoring cell proliferation and adhesion. In the Rat Model of L4-L5 Posterolateral Spinal Fusion, HA/PDHA/CHI PEM synergized with PSO greatly improved proliferation, adhesion and osteogenesis of rBMSCs, and promoted new bone formation, and therefore accelerated spinal fusion rate. This study provides a new treatment for spinal fusion therapy and may have great potential in bone regeneration and bone integration for implants.
研究领域:
生物医学工程
·
骨科手术
·
生物材料科学
·
干细胞生物学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
Theranostics
·
2019
·
IF 8.063
Qian Tang,Zhichao Hu,Haiming Jin,Gang Zheng,XingFang Yu,Gang Wu,Haixiao Liu,Zhenzhong Zhu,Huazi Xu,Changqing Zhang,Liyan Shen
Incorporating a biomimetic coating and integrating osteoinductive biomolecules into basic bone substitutes are two common strategies to improve osteogenic capabilities in bone tissue engineering. Currently, the underlying mechanism of osteoporosis (OP)-related deficiency of osteogenesis remains unclear, and few treatments target at OP-related bone regeneration. Herein, we describe a self-assembling polyelectrolyte multilayered (PEM) film coating with local immobilisation of calcitriol (Cal) in biphasic calcium phosphate (BCP) scaffolds to promote osteoporotic bone regeneration by targeting the calcium sensing receptor (CaSR). Methods : The ovariectomy-induced functional changes in bone marrow mesenchymal stem cells (BMSCs), protective effects of Cal, and the potential mechanism were all verified. A PEM film composed of hyaluronic acid (HA) and chitosan (Chi) was prepared through layer-by-layer self-assembly. The morphology, growth behaviour, and drug retention capability of the composite scaffolds were characterised, and their biocompatibility and therapeutic efficacy for bone regeneration were systematically explored in vitro and in vivo. Results : The osteogenic differentiation, adhesion, and proliferation abilities of ovariectomised rat BMSCs (OVX-rBMSCs) decreased, in accordance with the deficiency of CaSR. Cal effectively activated osteogenesis in these OVX-rBMSCs by binding specifically to the active pocket of the CaSR structure, while the biomimetic PEM coating augmented OVX-rBMSCs proliferation and adhesion due to its porous surface structure. The PEM-coated scaffolds showed advantages in Cal loading and retention, especially at lower drug concentrations. HA/Chi PEM synergised with Cal to improve the proliferation, adhesion, and osteogenesis of OVX-rBMSCs and promote bone regeneration and BCP degradation in the critical-size calvarial bone defect model of OVX rats. Conclusion : A composite scaffold based on BCP, created by simply combining a biomimetic PEM coating and Cal immobilisation, could be clinically useful and has marked advantages as a targeted, off-the-shelf, cell-free treatment option for osteoporotic bone regeneration.
研究领域:
生物医学工程
·
组织工程
·
骨再生
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Yonglin Yu,Qichun Ran,Xinkun Shen,Hong Zheng,Kaiyong Cai
After implantation into a host, titanium (Ti) orthopaedic materials are facing two major clinical challenges: bacterial infection and aseptic loosening, which directly determine the long-term survival of the implant. To endow Ti implant with self-defensive antibacterial properties and desirable osteo/angio-genic differentiation potentials, hyaluronic acid (HA)-gentamicin (Gen) conjugates (HA-Gen) and chitosan (Chi) polyelectrolyte multilayers were constructed on deferoxamine (DFO) loaded titania nanotubes (TNT) substrates via layer-by-layer (LBL) assembly technique, termed as TNT/DFO/HA-Gen. The HA-Gen conjugate was characterized by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance ( 1 H NMR). The physicochemical properties of the substrates were characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The on-demand DFO release was associated with the degradation of multilayers triggered by exogenous hyaluronidase, which indicated enzymatic and bacterial responsiveness. The TNT/DFO/HA-Gen substrates displayed effective antifouling and antibacterial properties against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), while were favourable for the adhesion, proliferation and osteo/angio-genic differentiation of mesenchymal stem cells (MSCs). The multifaceted drug-device combination (DDC) strategy showed potential applications in orthopaedic fields.
研究领域:
生物材料
·
组织工程
·
骨科
·
药剂学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）