ACS Applied Nano Materials
·
2022
·
IF 6.14
Liang Zhang,Li-Zong Su,Zhou Yu,Lu-Yi Su,Yi Zheng,Pei Song,Ya-Hao Wang,Yi-Bin Pan,Bin Zheng
Creatinine level in body fluid has been most used for early diagnosis of kidney function and renal diseases; therefore, it is desirable to develop a rapid and quantitative method for creatinine detection. Herein, surface-enhanced Raman spectroscopy (SERS) based on evaporation-induced optimal hotspots of Au nanocubes has been employed to quantitatively detect creatinine in human serum. Time-dependent SERS measurements during the evaporating process clearly prove that the optimal hotspots are formed on the eve of droplet drying of a Au sol and analyte mixture. The reproducibility tested by independent droplet experiments shows a relative standard deviation of SERS intensity of about 4.16%. Furthermore, the quantitative analysis of creatinine shows a clear linear fitting between the logarithmic SERS intensity and the creatinine concentrations with a correlation coefficient of 0.987. The creatinine level in the serum from volunteers estimated by this method shows an average difference of less than 5% compared to that by the clinical Jaffe reaction method. The evaporation-induced optimal hotspot method with good reproducibility offers a simple and feasible way for practical SERS detection.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Ning Wu,Hao Guo,Liping Peng,Yuan Chen,Lei Sun,Yinsheng Liu,Xiaoqing Wei,Wu Yang
Creatinine (Cre) measurement in biological fluids is an increasingly important clinical measure for evaluating renal insufficiency, thyroid dysfunction, and muscle injury. Therefore, a simple, fast, and reliable Cre analysis method is of great significance to human health. Herein, a novel ratiometric fluorescence sensor, Ag-Eu-MOF-808-EDTA, was synthesized by introducing ethylenediaminetetraacetic acid (EDTA), Ag + , and Eu 3+ into the framework of MOF-808 through three steps of post-synthesis modification, and was used to detect Cre in PBS buffer of pH 7.2 at 35 °C with a “turn-on” ratio response in the presence of various interfering ions and several biomolecules. In this strategy, the characteristic emission of Eu 3+ at 551 nm can be turned on due to coordination between Cre and Ag + , while emission at 613 nm is independent of Cre, which produces a significant ratio response change throughout the cycle. Experimental results showed that the ratio fluorescence sensor displayed a wide linear range for Cre from 0.5 to 350 μM with a low detection limit of 0.42 μM. Finally, a good recovery rate (96.7–102.9%) and appropriate relative standard deviation (RSD <2.39%, n = 3) were obtained during the determination of creatinine in real urine and serum. This work provides additional insights into the detection of creatinine in clinical blood samples.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2022
·
IF 6.76
Jiang Ling,Wenqi Zhang,Zijia Cheng,Yanjun Ding
Cantharidin (CTD), an effective component of Chinese herbal medicine with unique efficacy, caused poisoning or death constantly for incorrect use. Therefore, there is an intense need for a reliable, selective, sensitive and low-costing analytical method for monitoring the concentration of CTD in biological samples. In this work, a ratiometric fluorescent sensor (CdTe@MIPs/CDs@NIPs) was successfully constructed for selective determination of CTD by simply integrated the non-imprinted polymers coated CDs (CDs@NIPs) with cadmium telluride quantum dots coated with molecular imprinted polymers (CdTe@MIPs). CDs@NIPs were directly used as reference signal without addition of CTD templates and the as-prepared CdTe@MIPs were response signal. With the CTD addition, the fluorescence intensity of CdTe@MIPs decreased, while the fluorescence of CDs@NIPs remained approximately unchanged, resulting a detection limit as low as 0.15 nM. By spiked human blood samples, the ratiometric sensor was successfully applied to CTD detection with good recoveries of 96.12–107.40% and relative standard deviation (RSD) of 2.87–3.96%. Moreover, after centrifuging and re-eluting, the developed sensor could be effectively recycled and reused five times. Significantly, the proposed platform with low-cost and high-sensitivity opens a door towards the practical applications of ratiometric sensor and shows immense potential in pursuing the sensitive, reliable and low-cost determination of poisons in forensic toxicology analysis.
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RSC Advances
·
2022
·Vol. 12
·期 5
·3157-3164
·
IF 4.036
Yi Xiao,Guofang Wang,Haomin Yi,Suhua Chen,Qinyu Wu,Siyi Zhang,Kexin Deng,Simeng Zhang,Zi-Qi Shi,Xiaoping Yang
Ru(bpy)3Cl2/TPrA is a prominent and widely used ECL system in analytical science. However, the co-reactant TPrA restricts the variety of applications because of its toxicity, volatility, and high cost. Here, we use arginine (Arg) as an alternative co-reactant for Ru(bpy)32+ by taking advantage of its low cost, non-toxicity, and biocompatibility. The mechanism of the Ru(bpy)32+/Arg system is that the deprotonated Arg can react with Ru(bpy)32+ to release emission. The similarity between the Ru(bpy)32+/Arg, Ru(bpy)32+/TPrA, and Ru(bpy)32+/DBAE systems demonstrates that Arg can be used as an alternative co-reactant for Ru(bpy)32+ ECL. As a proof of concept, we achieve an excellent performance for acetaminophen (Ace) detection based on the specificity of Arg and Ace, with excellent linearity, low detection limits, and good recoveries. This work is promising to expand the scope of the Ru(bpy)32+/Arg system and move forward their applications in bioassays.
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MICROCHIMICA ACTA
·
2021
·Vol. 189
·期 1
·1-8
·
IF 5.833
Yang Jinlan,Gao Yulan,Yang Li
A novel capillary-based fluorescence microsensor for artemisinin was developed with functional polyoxometalates (POMs) as nanozyme by a layer-by-layer self-assembly strategy. Vanadomolybdophosphoric heteropoly acid ( H 5 PMo 10 V 2 O 40 , PMoV 2 ) and tungstophosphoric heteropoly acid ( Na 5 PW 11 O 39 Cu, PW 11 Cu) with high peroxidase-like activity were synthesized and immobilized on capillary to catalyze artemisinin/thiamine reaction and generate the amplified fluorescence signal. The wide linear range up to 13.0 μM with the low limit of detection of 0.03 μM (S/N = 3) was achieved for the determination of artemisinin by using the proposed POMs-microsensor. The method has been successfully used to detect artemisinin in human plasma and antimalarial drugs with satisfactory accuracy. This work developed a novel capillary fluorescence microsensor with functional POMs as nanozyme, which can serve as a promising candidate in fluorescence microanalysis. Graphical abstract
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ACS Applied Nano Materials
·
2021
·
IF 5.097
Siying Liu,Hai-Sheng Su,Zhengxia Yang,Yun Zhang
The identification of cancer cells and tumors significantly advances the accurate diagnosis of cancer and improves the patient survivor rates. However, it remains challenging to achieve the rapid and label-free differentiation of cancer cells from their normal counterparts. Herein, we developed a rapid and effective method for the synthesis of Ag nanorods (NRs) with high uniformity and yield. The surface-enhanced Raman scattering (SERS) substrates demonstrated uniformity and reliability, with a low relative standard deviation (RSD) of 10% and an enhancement factor (EF) of 3.32 × 105. The optimized AgNR SERS substrates were then applied in the quantitative analysis of creatinine and furthermore the bioanalysis of cell lysates for cell classification. Specifically, a high sensitivity with a low detection limit (LOD) of 5.3 μM and a limit of quantification (LOQ) of 17.68 μM was achieved for the detection of creatinine. More importantly, label-free SERS analysis showed the distinct spectral features of the cell lysates for normal breast cell strains (MCF-10A) and breast cancer cell strains (MCF-7, MDA-MB-231). Statistical analysis, such as t-test, principal component analysis (PCA), and orthogonal partial least-squares discriminant analysis (OPLS-DA) aided in the clear discrimination between the normal and cancer groups and further in differentiating the two cancer groups. These results demonstrate the great potential of the combination of a high-quality AgNR SERS substrate and statistical analysis for the identification of cancers.
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BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Tianyu Li,Bo Liang,Zhichao Ye,Lei Zhang,Shiyi Xu,Tingting Tu,Yiming Zhang,Yu Cai,Bin Zhang,Lu Fang,Xiyu Mao,Shanshan Zhang,Guan Wu,Qifu Yang,Congcong Zhou,Xiujun Cai,Xuesong Ye
Simultaneous monitoring of electrophysiological and biochemical signals is of great importance in healthcare and fitness management, while the fabrication of highly integrated and flexible devices is crucial to these applications. Herein, we devised a multifunctional and flexible hydrogel-paper patch (HPP) that was capable of simultaneously real-time monitoring of electrocardiogram (ECG) signal and biochemical signal (glucose content) in sweat during exercise. The self-assembly of the highly porous PEDOT:PSS hydrogel on paper fiber provided the HPP with good conductivity and hydrophilic wettability for efficient electron transmission and substance diffusion, thereby enabling it to serve as a low-impedance ECG electrode and a highly sensitive glucose sensor . Additionally, the spontaneous capillary flow effect allows the paper patch to be used as microfluidic channels for the collect and analysis of sweat. Moreover, the HPP is integrated with a flexible printed circuit board (FPCB) and works as a multifunctional wearable device mounted on the chest for real-time monitoring of electrophysiological and biochemical signals during exercise.
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PEST MANAGEMENT SCIENCE
·
2023
·Vol. 80
·期 4
·1868-1876
·
IF 4.1
Xiaoqin Cheng,Qiang Wen,Yunna Li,Shanshan Wang,Gang Fan,Zhaocheng Ma,Yuancheng Guo,Xiaoxue Li,Hongyu Zhang
BACKGROUND Bactrocera minax is a devastating pest of citrus fruits. However, there have been no effective control measures before. Few reports on the sex pheromones of B. minax are available. RESULTS In this study, nine of the volatile compounds in adult females were identified using headspace solid-phase microextraction (HS-SPME) in combination with gas chromatography–mass spectrometry (GC–MS). Among them, d-limonene, caprolactam, 2-Nitro-1H-imidazole, and creatinine could evoke antennal responses in males. Field bioassays showed that only d-limonene could lure male flies, with a relative lure rate of 78.18% in all tested samples, which was significantly higher than that of paraffin oil control, while all volatile compounds did not have any lure effective to female flies. Moreover, d-limonene was diluted with paraffin oil into differential concentrations, the lure effect on males was better at 100, 500, and 800 μL d-limonene mL −1 than pure d-limonene (1000 μL mL −1 ). The relative male lure rate of d-limonene at 100 μL mL −1 was 85.88%, which was significantly higher than that of food−baits (14.12%) on day 3. However, d-limonene was unattractive to female and male Bactrocera dorsalis and Zeugodacus tau . Further kinetic analysis showed that female adults released d-limonene around 15-day post eclosion. Electroantennography 1 results showed that 500 μL mL −1 d-limonene evoked the strongest responses to antennae of 10- to 25-day-old male flies. CONCLUSION Our results indicated that d-limonene could be a sex pheromone from female flies of B. minax , and it could be used as a male-specific sex attractant for B. minax. © 2023 Society of Chemical Industry.
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Xueping Quan,Bing Yan
Metal-organic framework (MOF) and covalent-organic framework (COF) hybrid materials can combine the unique properties of MOF and COF components, and their applications in fluorescence sensing have attracted more and more attention. Herein, ZIF-90 is grown on 3D-COF by a simple in situ growing method in which the 7-amino-4-methylcoumarin (AMC) is encapsulated in ZIF-90 to construct a fluorescent sensor. Chloroquine phosphate (CQP) can coordinate with Zn2+ to decompose the ZIF-90 and release AMC. At 365 nm excitation, the ratiometric fluorescence signal AMC/3D-COF (I430/I598) increases linearly with CQP in a linear range of 4 × 10–5 to 4 × 10–4 M in urine. Under 340 nm excitation, quantitative analysis of CQP in the serum (3 × 10–6 to 4 × 10–5 M) is based on the fluorescence intensity of Zn-CQP/3D-COF (I384/I598). In addition, AMC@ZIF-90/3D-COF (1) exhibits high anti-interference and selectivity in sensing of FA with a “turn off” mode, with a correlation range of 1 × 10–5 to 1 × 10–3 M. The fluorescence color changes triggered by CQP under different excitation conditions, and the different fluorescence responses caused by CQP make it a highly secure anticounterfeiting platform. The synthesized dye@MOF/COF hybrids not only provide a new way to integrate multiple emission to design fluorescent probes for differentiation detection but also offer ideas for the design of anticounterfeiting platforms.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 50
·109110-109122
·
IF 5.8
Liu Jiadi,Dong Yawen,Kang Yu,Kong Qian,Wang Kuaibing,Mao Feifei,Bu Yuanqing,Zhou Rong,Zhang Chunyong,Wu Hua
Developing multifunctional catalysts applied in diversiform modes via advanced oxidation processes (AOPs) is a promising and attractive approach for organic pollution degradation. Herein, a novel hollow bamboo-like structural cobalt/nitrogen-doped carbonized material (CoC/N) was employed as a catalyst for AOPs, in which CoC/N was prepared in situ through calcining a Co-based coordination polymer. When CoC/N was utilized as a peroxymonosulfate (PMS) activator, the catalyst stood out prominent activities for effective CA oxidation. Furthermore, a five-level central composite rotatable design (CCRD) model describing CA decay as a function of PMS concentration, CoC/N dosage, and solution pH value was successfully constructed and engaged to explore the optimal operating conditions. Finally, the possible degradation mechanism of CA in CoC/N-PMS system was proposed by quantum chemistry calculation and LC/MS analysis. This work shed light on the structural morphology of the catalyst and its PMS synergy degradation pathway, which promotes its applications in miscellaneous pollutant degradation. Graphical Abstract A new Co/N-doped material was used to degrade unconventionality organic pollutant creatinine (CA) for the first time, in which the scientific approaches of five-level central composite rotatable design (CCRD) model, response surface methodology (RSM) and density function theory (DFT) were employed to evaluate the material performance and CA degradation pathway. The toxicity evaluation, statistical modeling and mechanisms study have been investigated meticulously
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Wei Jia,Jiying Zhu
ε-Polylysine is a novel food preservative approved by the U.S. Food and Drug Administration (FDA), yet the mechanism of its effect on animal-derived foods remains unclear. Assessment of the effect of preservatives on goat meat products is necessary. Herein, metabolite accumulation and protein expression of ε-polylysine (0.025%, w/w) spiked with goat meat were investigated by nontarget metabolomics and proteomics combined with ultrahigh performance liquid chromatography quadrupole-Orbitrap high-resolution-mass spectrometry (UHPLC-Q-Orbitrap HRMS) in a simulated in vitro digestion model. The amino side chain of ε-polylysine increased the activity of glycine aminotransferase due to its nucleophilic nature, inducing a significant upregulation of l-arginine (0.43–0.72 mg kg–1) and creatine (3.98–6.89 mg kg–1), with an improvement in muscle quality of goat meat. Downregulation of enzyme phenylalanine hydroxylase expression led to upregulation of l-phenylalanine (2.26–3.25 mg kg–1) and l-tyrosine (0.98–1.29 mg kg–1). Collectively, this study first revealed the biochemical mechanism of ε-polylysine in goat meat products, which makes available new prospects for more accurate use of ε-polylysine in animal-derived foods.
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ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Kaibo Xu,Liqin Cao,Zhouyu Wang,Liu-ping Chen
Hemoperfusion (HP) is one of the most prominent therapies for treating uremia, hyperbilirubinemia, and acute drug toxicity. The comprehensive performance of currently used porous HP adsorbents needs to be improved due to the impediment to their synthesis strategy. Herein, green carbon dioxide-in-water high internal phase emulsions (C/W HIPEs) were utilized and emulsified with poly(vinyl alcohol) (PVA) for the formation of a heparin-mimetic chitosan oligosaccharides/poly(acrylamide-co-sodium 4-styrenesulfonate) [COS/P(AM-co-SSS)] monolith, which exhibited good mechanical properties, stable swelling performance, hydrophilic properties, anticoagulant effect, and low hemolysis. It showed a strong toxin adsorption capacity (415.2 mg/g for creatinine, 199.3 mg/g for urea, 279.5 mg/g for bilirubin, and 160 mg/g for tetracycline). The adsorption process of porous COS/P(AM-co-SSS) followed the pseudo-second-order kinetic and Langmuir isotherm models. Moreover, the porous materials had a strong electrostatic force on creatinine. The removal of creatinine by simulated in vitro blood perfusion was 80.2% within 30 min. This work provides a green preparation strategy for developing novel HP materials, highlighting their potential application value in blood and environmental purification.
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