JOURNAL OF PHYSICAL CHEMISTRY B
·
2025
·
IF 2.9
Lei Wang,Zhen-Zhen Li,Gong-Ji Chen,Mi-Zhuan Li,Yan Cheng,Zhong-Xiu Chen
The activity of lipase was inhibited or promoted by different macromolecular colloids, but the underlined general rules were not clear. In this paper, lipase activity was systematically analyzed in different macromolecular media. The detailed factors that affect the enzyme activity were extracted for quantification. Three functions, including viscosity, the crowding effect, and soft interactions, were established to describe the macromolecular medium effect. The medium parameter based on the Huggins constant and Kramer constant was able to characterize the quality of the macromolecular media. A multivariate nonlinear regression model was proposed for the correlation or prediction between three groups of factors and enzyme activity, and a crowding enzyme factor CCef was proposed. This study pioneered the qualification and prediction of the multiple factors that affect the enzyme activity in complex media. It is also significant for scientifically predicting the efficacy of functional macromolecules related to healthy food and the formulation of dietary fibers.
研究领域:
生物化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Zhong Li,Huilin Zhou,Xuan Liu,Weifei Wang,Dongming Lan,Yonghua Wang
The limited availability of phospholipase A 1 (PLA 1 ) has posed significant challenges in enzymatic degumming. In this study, a novel PLA1 (UM2) was introduced to address this limitation, which had a unique thermo-responsive ability to switch phospholipase and lipase activities in response to temperature variations. Remarkably, UM2 displayed an unprecedented selectivity under optimized conditions, preferentially hydrolyzing phospholipids over triacylglycerols—a specificity superior to that of commercial PLA 1 . Moreover, UM2 demonstrated high efficiency in hydrolyzing phospholipids with a predilection for phosphatidylcholine (PC) and phosphatidylethanolamine (PE). A practical application of UM2 on crude flaxseed oil led to a dramatic reduction in phosphorus content, plummeting from an initial 384.06 mg/kg to 4.38 mg/kg. Broadening its industrial applicability, UM2 effectively performed enzymatic degumming for other distinct crude vegetable oils with a unique phospholipid composition. Collectively, these results highlighted the promising application of UM2 in the field of oil degumming.
研究领域:
生物化学
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食品科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Junxin Zhao,Maomao Ma,Xianghui Yan,Dongman Wan,Zheling Zeng,Ping Yu,Deming Gong
The lipase from Bacillus licheniformis NCU CS-5 was immobilized onto β -cyclodextrin (CD) grafted and aminopropyl-functionalized chitosan-coated Fe 3 O 4 magnetic nanocomposites (Fe 3 O 4 -CTS-APTES-GA- β -CD). Fourier transform infrared spectroscopy, thermogravimetry analysis, X-ray diffraction, scanning electron microscopy and transmission electron microscopy showed that not only the functionalized magnetic nanoparticles were synthesized but also the immobilized lipase was successfully produced. The immobilized lipase exhibited higher optimal pH value (10.5) and temperature (60℃) than the free lipase. The pH and thermal stabilities of the immobilized lipase were improved significantly compared to the free lipase. The immobilized lipase remained more than 80% of the relative activity at temperature of 60 ℃ and pH 12.0. The immobilized lipase also remained over 80% of its relative activity after 28 days of storage and 15 cycles of application. The application of the immobilized lipase in esterification of isoamyl acetate and pentyl valerate showed that maximum esterification efficiency was achieved in n -hexane having 68.0% and 89.2% respectively. Therefore, these results indicated that the Fe 3 O 4 -CTS-APTES-GA- β -CD nanoparticles are novel carriers for immobilizing enzyme, and the immobilized lipase can be used as an innovative green approach to the synthesis of fruity flavor esters in food industry.
研究领域:
生物化学
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食品科学
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纳米技术
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 7
·4052-4064
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IF 3.612
Cheng-wei Yu,Ting Luo,Tian Xie,Jing Li,Ze-yuan Deng
Summary Rice grains were obtained from two Chinese provinces (Hubei and Guangxi) and then their rice bran was abraded into four equal fractions by weight from outside to inside. The component distributions in these four fractions were then investigated. The contents of total lipid-soluble antioxidant showed no significant difference between rice bran from the Hubei province and the Guangxi province. But the content of total water-soluble antioxidant was 1690.20 ± 86.40 mg kg −1 for rice bran from the Hubei province, significantly higher than those from the Guangxi province (1014.67 ± 71.09 mg kg −1 ). Obvious differences between four rice bran fractions or two provinces were proved by principal component analysis (PCA). Lipase activities decreased significantly from outer fractions to inner fractions, and lipids mainly distributed in three outer fractions. The essential element contents decreased significantly, but a main heavy metal element (Pb) in cereal increased significantly to 0.36 mg kg −1 from outer fractions to inner fractions, which was just slightly below the upper limit of Chinese Standard (0.40 mg kg −1 ). Although protein contents had no significant difference in four fractions, the amino acid compositions were quite different. In summary, different fractions of rice bran had different compositions, which could provide guidance for the classified processing of rice bran.
研究领域:
食品科学
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营养学
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
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IF 3.3
He Ying,Huang Ao,Liu Yun
Phospholipase D (PLD) is a valuable enzyme in industrial processes for converting phosphatidylcholine (PC) to phosphatidylserine (PS). In this study, a strain of Bacillus cereus was isolated from soil and identified through 16S rRNA sequencing. To enhance PLD activity, various mutagenesis strategies—including chemical treatment, irradiation, and their combinations—were employed, resulting in four high-activity positive mutants (C-7, I-12, CI 7–12, and IC 13–14). Among these, the CI 7–12 mutant exhibited a significantly higher enzymatic activity, showing a 3.12-fold increase (312.2%) compared to the wild-type strain. Fermentation conditions were optimied using response surface methodology (RSM), achieving a PLD activity of 35 U/mL. The enzyme demonstrated stability over a wide temperature range (30–60 °C) and pH range (6–10), with a half-life of 128 days. Kinetic analysis revealed a V max of 20.04 μmol/h and a K m of 7.13 μmol/mL, indicating efficient activity. In bioconversion experiments, the PLD-enriched fermentation broth catalyzed the conversion of PC to PS, achieving a 53.0% conversion rate and a 92.3% selectivity for PS in a two-phase system. These findings expand the potential sources of PLD and underscore its applicability for PS production in biotechnological applications.
研究领域:
微生物学
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生物化学
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工业生物技术
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Zhe Dong,Zengshe Liu,Jie Shi,Hu Tang,Xia Xiang,Fenghong Huang,Mingming Zheng
A sustainable and efficient reaction system for enzymatic oil modification is imperative for the food industry. Given the interfacial activation effect of lipase, the sufficient oil–water interface is vital for excellent catalytic activity of lipase. In the present study, a Pickering emulsion reaction system was prepared through directly using reactants as the oil phase and stabilized by mesoporous carbon spheres (MCS) after immobilization of lipase AYS (Candida rugosa, lipase@MCS). Lipase@MCS worked as both the emulsifier and catalyst. Each droplet in the Pickering emulsion was regarded as a microreactor with the reactants in the oil phase, and the removal of the product H2O “pushed” the reaction forward. Taking advantage of the adequate oil–water interface for “lid” opening of lipase and rapid mass transfer in MCS, the Pickering emulsion exhibited excellent enzymatic activity toward phytosterol esterification and transesterification, achieving 95.0% conversion after 1.5 h and catalytic efficiency (CE) of 6.8 mmol g–1 h–1 under nonequilibrium conditions. It is the best performance reported currently with the shortest reaction time, minimal amount of catalyst, and the highest CE (19-fold higher than free lipase one-phase reaction system). Immobilization of lipase on the MCS not only endowed the lipase with exceptional recyclability but also afforded a shield to protect the enzyme from deactivation in a harsh environment, as evidenced by the thermotolerance and recycling test results. This paper paves the way to advancing progress in enzyme-immobilized Pickering emulsion as a sustainable and high-efficiency platform for biocatalysis application.
研究领域:
生物化学
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食品科学
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材料科学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2016
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IF 2.628
Dun Deng,Yun Zhang,Aijun Sun,Yunfeng Hu
We previously functionally characterized a novel marine microbial GDSL lipase MT6 and identified that the stereo-selectivity of MT6 was opposite to that of other common lipases in trans-esterification reactions. Herein, we have investigated the use of MT6 in stereo-selective biocatalysis through direct hydrolysis reactions. Notably, the stereo-selectivity of MT6 was also demonstrated to be opposite to that of other common lipases in hydrolysis reactions. Parameters, including temperature, organic co-solvents, pH, ionic strength, catalyst loading, substrate concentration, and reaction time, affecting the enzymatic resolution of racemic 1-phenylethyl acetate were further investigated, with the e.e. of the final ( S )-1-Phenylethanol product and the conversion being 97% and 28.5%, respectively, after process optimization. The lengths of side chains of 1-phenylethyl esters greatly affected the stereo-selectivity and conversion during kinetic resolutions. MT6 is a novel marine microbial GDSL lipase exhibiting opposite stereo-selectivities than other common lipases in both trans-esterification reactions and hydrolysis reactions.
研究领域:
生物化学
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微生物学
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2021
·
IF 3.757
Yangyang Cai,Shuqi Xing,Qifeng Zhang,Ruonan Zhu,Kai Cheng,Cuiqin Li,Xuefeng Zeng,Laping He
The extracellular Aspergillus niger GZUF36 lipase (EXANL1) has broad applicability such as synthesis of functional lipids. The wild-type strain is unstable, and the culture time is long. Studies on the selectivity of substrates for EXANL1 are not available. Therefore, this study aimed to express, purify, characterize and analyze the substrate specificity of EAXNL1. The results showed that EXANL1 was stable in the presence of 40 % ( v/v ) dimethyl sulfoxide, glycerol, toluene, and n-hexane. The relative activity was 104 %, 88 %, 81 %, and 76 %. The residual enzyme activity of EXANL1 was more than 85 % in surfactants with a low concentration. EXANL1 exhibited a high conversion rate (79 %) for p -nitrophenyl palmitate. The molecular docking of p -nitrophenyl palmitate into the active site of EXANL1 indicated that the preference for this fatty acid chain length might be due to the formation of hydrogen bonds. The distance between the carbonyl carbon atom of p -nitrophenyl palmitate and the nucleophilic oxygen atom of serine 162 was 2.91 Å. Both experiments revealed that EXANL1 exhibited higher specificity for p -nitrophenyl palmitate. These results indicated that EXANL1 could be a promising tool in organic synthesis and detergent industry. The molecular docking provided a basis for the explanation on the substrate selectivity of EXANL1.
研究领域:
生物化学
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酶学
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工业微生物学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Xinmiao Wang,Qian Wang,Dong Cai,Xu Guo,Peiyong Tong,Fawen Yin,Xiaoyang Liu,Dayong Zhou
Phenolipids such as hydroxytyrosol-SCFA acyl esters (HTy-SEs) and tyrosol-SCFA acyl esters (TYr-SEs) with various alkyl chains lengths (C1–C4) and different isomers (branched-chain and straight-chain) were successfully synthesized. All esters were hydrolyzed by pancreatic lipase to produce polyphenols (HTy and TYr) and SCFAs (iso-butyric acid, acetic acid, propionic acid, and n-butyric acid). Moreover, HTy-SEs (and TYr-SEs) could also be hydrolyzed to free HTy (and TYr) and SCFAs by gut microbiota and Lactobacillus from mice feces. Especially, the hydrolysis rates showed positive correlation with the carbon skeleton length, and the hydrolysis degree (DH) of ester with a branched-chain fatty acid was weaker than that of ester with a straight-chain fatty acid. Besides, the DH values of TYr -SEs were significantly higher than those of HTy-SEs. Therefore, through regulating the structures of polyphenols, carbon skeleton lengths, and isomers, controlled-release of polyphenols and SCFAs from phenolipids will be easily achieved.
研究领域:
食品科学
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营养学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xiaoqiang Zou,Mudassar Hussain,Imad Khan,Yanxi Wang,Bangzhi Jiang,Lei Zheng,Yuechao Pan,Jijie Hu,Azqa Ashraf
Medium- and long-chain structured lipids (MLSLs) rich in docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were synthesized by acidolysis of fish oil (Omax 1812, a mixture of sardine and anchovy oils) with caprylic acid (CA) using a lipase bio-imprinted with fatty acids as a catalyst. A bio-imprinted lipase for the acidolysis reaction was first prepared under optimal conditions, and its activity was 1.43 times higher than that of the non-bioimprinted. The bio-imprinted lipase was used as a catalyst to produce such MLSLs, and the reaction conditions were optimized. Under the optimal conditions, equilibrium was achieved in 8 h, and the total and the sn -1, 3 CA contents in the obtained product were 28.72% and 42.26%. Additionally, the total and the sn -2 DHA contents were 14.23% and 21.10%, respectively, and the total and the sn -2 EPA contents were 16.43% and 10.14%, respectively. These obtained MLSLs, rich in sn -1, 3 CA, and sn -2 DHA, EPA can deliver significant health benefits.
研究领域:
生物化学
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食品科学
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产品关联
DOI
高影响因子
3 Biotech
·
2021
·Vol. 11
·期 4
·1-9
·
IF 2.406
Ren Runxian,Zhai Lixin,Tian Qiaopeng,Meng Di,Guan Zhengbin,Cai Yujie,Liao Xiangru
Diphenyl phosphate (DPHP) has been increasingly detected in environmental samples, posing a potential hazard to humans and other organisms and arousing concern regarding its adverse effects. Biological degradation of DPHP is considered a promising and environmentally friendly method for its removal. In this study, the bagdpd gene was mined from the Bacillus altitudinis W3 genome and identified as a glycerophosphodiester phosphodiesterase by bioinformatics analysis. The enzyme was expressed and its biochemical properties were studied. When using bis(4-nitrophenyl) phosphate as substrate, enzyme activity was optimal at 55 °C and a pH of 8.5. The enzyme remained stable in the pH range of 8.0 − 10.0. The rBaGDPD enzyme degraded DPHP and the reaction product was identified as phenyl phosphate by LC–MS. This is the first report of a glycerophosphodiester phosphodiesterase exhibiting hydrolytic activity against DPHP. This study demonstrated that rBaGDPD could have the potential for bioremediation and industrial applications.
研究领域:
环境科学
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微生物学
·
生物化学
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DOI
PubMed
高影响因子
Molecular Catalysis
·
2025
·
IF 4.9
Duan Zhou,Mouming Zhao,Xiaorong Zhong,Xuefeng Chen,Ning Liu,Daoming Li
Diacylglycerol (DAG) has extensive applications in many fields due to its health benefits and amphiphilic property. Herein, enzymatic esterification of oleic acid and glycerol in natural deep eutectic solvents (NADESs) was employed to synthesize DAG. Firstly, Novozym 435 and choline chloride: glycerol (ChCl:Gly) system were selected as the most appropriate immobilized lipase and NADES for DAG synthesis. Subsequently, the reaction conditions of Novozym 435-catalyzed esterification in ChCl:Gly system were optimized, and the content of DAG reached as high as 67.08 wt % ± 0.79 wt % under the optimal conditions (molar ratio of oleic acid to glycerol of 1:4, reaction temperature of 50 °C, enzyme dosage of 4 wt % with respect to the total weight of substrates, reaction time of 7 h). At last, the reasons why ChCl:Gly could induce a higher DAG content were explored from thermodynamics, water activity and reaction interface. The results showed that ChCl:Gly system could absorb the generated water during esterification and increase the reaction interface, thus beneficial to DAG synthesis. Moreover, the activation energy of DAG production (55.36 kJ/mol) was significantly lower than that of monoacylglycerol (MAG) production (62.60 kJ/mol), leading to the priority of DAG production. Briefly, the multiple beneficial effects of NADESs on lipase-catalyzed esterification collectively contributed to an enhancement of DAG content in esterification products.
研究领域:
生物化学
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食品科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）