Antioxidants
·
2023
·Vol. 12
·期 3
·606
·
IF 7
Ziyi Yang,Jiaming Cai,Evans Frimpong Boateng,Lujuan Xing,Wangang Zhang
This present study aimed to investigate the effects of various cooking times (1 h, 1.5 h, 2 h, 2.5 h, named as JHBP-1, JHBP-1.5, JHBP-2, JHBP-2.5) on the antioxidant activity and peptide profile of Jinhua ham broth peptides (JHBP). The peptides extracted from uncooked ham were used as an uncooked group with the name of JHBP-0. The results revealed that the antioxidant efficacy in the four cooked groups changed dramatically compared to JHBP-0. After cooking, the DPPH radical scavenging activity, hydroxyl radical scavenging activity and superoxide anion radical scavenging activity decreased, except for the Fe2+chelation and ABTS+scavenging capacity which increased significantly. However, the cooked groups still showed a strong antioxidant capacity. In particular, the superoxide anion radical scavenging ability and the Fe2+chelation action were significantly stronger compared to glutathione (GSH) and butylated hydroxytoluene (BHT) (p< 0.05). JHBP-1.5 also displayed stronger antioxidant capacity than the other three cooked groups, and its secondary structure and mass distribution changed significantly after cooking, specifically with an increased proportion of helix and <1 kDa peptides. Moreover, the constitution of free amino acids (FAAs) and the types of peptides released in the broth increased significantly with a longer cooking time. In total, 1306 (JHBP-0), 1352 (JHBP-1), 1431 (JHBP-1.5), 1500 (JHBP-2), and 1556 (JHBP-2.5) peptide sequences were detected using LC-MC/MC. The proportion of <1 kDa peptides also gradually increased as the cooking time extended, which is consistent with the molecular weight distribution measurements.Keywords:Jinhua ham;broth;bioactive peptides;antioxidant activity;peptide profile
研究领域:
食品科学
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生物化学
·
营养学
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产品关联
DOI
PubMed
高影响因子
EXPERIMENTAL PARASITOLOGY
·
2023
·
IF 2.1
Muhammad Ehsan,Rui-Si Hu,Meng Wang,Jun-Ling Hou,Muhammad Rashid,Muhammad Irfan Malik
Legumains belonging to C_13 peptidase family of proteins, and are ubiquitously disseminated among all vertebrate and invertebrate organisms, and have been implicated in innumerable biological and cellular functionality. Herein, we characterized and evaluated immunoregulatory characteristics of Legumain-1 from Fasciola gigantica (Fg-LGMN-1) during its interaction with host immune cells . The isopropyl-ß-d-thiogalactopyranoside (IPTG) stimulated RFg-LGMN-1 protein was positively detected by rat serum containing anti-RFg-LGMN-1 polyclonal antibodies . Furthermore, the uptake of RFg-LGMN-1 by goat monocytes was successfully confirmed using Immunofluorescence Assay (IFA). The immunohistochemical analysis revealed the native localization of LGMN-1 protein on the periphery and internal structures such as suckers, pharynx, and genital pore of the adult parasite, thereby validating its presence in excretory-secretory (ES) products of F. gigantica . The RFg-LGMN-1 co-incubated with concanavalin-A (Con-A) stimulated the increase of interleukin 2 (IL-2), IL-10, and IL-17 in monocytes derived from peripheral blood mononuclear cells (PBMCs) in the concentration-dependent manner. However, the IL-4 cytokine in response to the RFg-LGMN-1 protein declined. These results illuminated the role of LGMN-1 during the parasite-host interface. Our findings elaborated additional evidence that Legumain protein play a role in the manipulating host immune responses during parasite infections. However, further evaluation of RFg-LGMN-1 protein in context of its immunomodulatory roles should be conducted to enhance our understandings of the mechanisms employed by F. gigantica to evade host immune responses.
研究领域:
免疫学
·
寄生虫学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Huanhuan Li,Ke Zhao,Jin Zhang,Lihong Chen,Qicheng Huang,Xiaozhong Ma,Shengyuan Ge,Honggang Tang
In the present study, we evaluated the physicochemical and microbial properties, microbial diversity, and volatile organic compounds (VOCs) of Jinhua ham prepared using Taihu black pigs during a ripening period of 210 days. During the eight timepoints, the protein oxidation, proteolysis indexes, and TBARS values of Jinhua ham had different change trends. The growth of total bacteria and total Staphylococcus showed a decline, while the growth trend of total fungi was parabolic. Staphylococcus equorum was the dominant bacterial species, and the relative abundance were 19.3% (day 180)-76.7% (day 120). Debaryomyces hansenii and Eurotium sp. were the dominant fungus species, with opposite change trends. GC–IMS analysis detected 84 volatile compounds, with alcohols and aldehydes being the dominant volatile compounds in the late ripening period. The correlation analysis between microbial diversity and VOCs showed that Staphylococcus equorum was significantly negatively correlated with eight VOCs, while Debaryomyces hansenii and Eurotium sp. were significantly correlated with 31 and 29 VOCs respectively. Our findings reveal the quality, microbial diversity at species level, volatile compound characteristics, and relationship between microbial communities and flavor formation of Jinhua ham prepared using Taihu black pigs.
研究领域:
食品科学
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微生物学
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分析化学
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Yehua Liu,Hongying Li,Mingming Li,Linggao Liu,Kuan Lu,Shenghui Bi,Mixin Zhou,Li Chen,Lisha Lan,Minping Wei,Ying Zhou,Qiujin Zhu
This study explored the effect of electrical stimulation (ES) and Pediococcus pentosaceus LL-07 ( P. pentosaceus LL-07) and Staphylococcus simulans QB7 ( S. simulans QB7) on the quality and microbial community of loin ham during the ripening. After the ES and starter culture treatments, the Aw and pH were decreased. Surface hydrophobicity, myogenic fiber fragmentation index (MFI), TCA-soluble peptide, amino nitrogen and free amino acids (FAAs) were also significantly higher than the control group (CK) ( P < 0.05). This increase was more significant in the E-S group (electrical stimulation followed by inoculation with P. pentosaceus LL-07 and S. simulans QB7) than the rest of the experimental group (E、S、S-E group). Furthermore, the CK and E-S groups were subjected to a bacterial community comparison experiment. The microbial diversity of these two groups was increased. Pediococcus spp. and Staphylococcus spp. became the dominant bacteria in E-S groups during the ripening. Correlation analyses show a strong correlation between protein hydrolysis, microorganisms and FAAs. In conclusion, the combination of ES and starter culture could promote protein hydrolysis, the accumulation of FAAS, and improves the bacterial community of loin ham.
研究领域:
食品科学
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微生物学
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生物化学
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DOI
PubMed
高影响因子
Materials Today Communications
·
2024
·
IF 3.7
Zihe Hu,Gaoying Hong,Mumian Chen,Yanyan Zhou,Haiyan Wu,Weiying Lu,Minghao Zuo,Zhijian Xie,Changyu Shao,Jue Shi
Excessive blood loss caused by natural accidents or major surgical procedures can be life-threatening and requires rapid hemorrhage management. Developing hemostatic materials with high water absorption capacity and high strength properties is still challenging. Super hydrophilic/high-strength chitosan hemostatic sponges (SHHS-CHS) were prepared by freeze-drying and alkali treatment. The freeze-drying technology formed a porous three-dimensional structure of sponges, and alkali treatment increased the hydrophilicity of sponges and the rigidity of the chitosan molecular chain, improving the hydrophilicity and mechanical properties of chitosan sponges. Characterization experiments revealed that SHHS-CHS had high water absorption capacity, rich pore structure, and excellent mechanical properties. The cytotoxicity and hemolysis experiments showed that SHHS-CHS had good biocompatibility. Compared to the commercial hemostatic gelatin sponges and chitosan hemostatic material, SHHS-CHS had better blood coagulation and red blood cell and platelet adhesion ability. Moreover, SHHS-CHS showed better hemostatic ability than gelatin sponges in rat-liver injury and rat leg vein rupture models, significantly reducing the hemostasis time and bleeding volume. In conclusion, SHHS-CHS has broad prospects for clinical application as a hemostatic material.
研究领域:
材料科学
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生物材料
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止血
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生物医学工程
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产品关联
DOI
高影响因子
FOOD CONTROL
·
2019
·
IF 4.248
Wenjing Yan,Wenbin Chen,Umair Muhammad,Jianhao Zhang,Hong Zhuang,Guanghong Zhou
The aim of this work was to prepare a novel antioxidant film by incorporating α-tocopherol-chitosan nanoparticles (TOC-CSNPs) with chitosan/montmorillonite film (namely, TOC-CSNPs/CS/MMT), and investigate the antioxidant activity of TOC-CSNPs/CS/MMT film on sliced dry-cured ham in a period of 120 days at 4 °C. Positively charged TOC-CSNPs with the average sizes of 214 nm were prepared using TPP-initiated ionic gelation mechanism. The amount of TOC-CSNPs was optimized based on the changes of water vapor permeability (WVP), water solubility and swelling ratio of the film. The TOC-CSNPs/CS/MMT film showed 46.5% radical scavenging activity after 16 days of storage, which was 23 times and 27 times higher than that of CS/MMT and CS film, respectively. The antioxidant activity of TOC-CSNPs/CS/MMT film was evaluated by measuring the peroxide value (POV) and thiobarbituric acid reactive substances (TBARS) values of sliced dry-cured ham coated with the TOC-CSNPs/CS/MMT film-forming solutions. The results indicated that compared with the CS (0.42 g/100 g for POV, 0.65 mg/kg for TBARs) and CS/MMT (0.38 mg/kg for POV, 0.59 mg/kg for TBARs) films, the biceps femoris muscle of the sliced ham coated with TOC-CSNPs/CS/MMT film showed much lower values of POV (0.15 g/100 g) and TBARs (0.15 mg/kg) during 120 days of storage. These results provide a theoretical basis for developing efficient and long-term antioxidant approach, which is crucial for improving and enhancing the quality of food products.
研究领域:
食品科学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2022
·
IF 15.304
Ke Li,Ruxiang Li,Pengfei Zou,Li Li,Huajun Wang,Deqian Kong,Guangying Zheng,Li-Li Li
Efficient and non-invasive drug delivery to the fundus has always been a medical difficulty. Here, a co-assembled glycopeptide nanotransforrs (GPNTs) named [email protected] as a drug delivery system is reported. The [email protected] co-assemble nanoparticles consisting of glycopeptide, cationic peptide, and doxorubicin (DOX). The nanoparticles are positively charged with the nano-size, which can be induced transformation by legumain cleavage. Once administrate to the eyes, [email protected] has a high penetration through the ocular surface to specifically targets M2 macrophages in the fundus. Then, the mannose receptor mediates phagocytosis and intracellular highly expressed legumain induces its nanofibrous transformation, which contributes to a 44.7% DOX retention in cells at 24 h than that of the non-transformed controls ( [email protected] : 5.1%). The nanofiber transformation provides an inhibition of exocytosis, which explains the higher retention of the delivered drug. In the mouse OIR model, [email protected] completely restores the physiological angiogenesis and reduces pathological neovascularization . Pathological neovascularization branches and cell nuclei that break through the inner limiting membrane are reduced by 55% and 72%, respectively, which are 25% and 20% less than those in the non-transformed controls. In addition, [email protected] also has good histocompatibility , which provides a possible strategy for non-invasive treatment of fundus diseases in the future.
研究领域:
药学
·
纳米技术
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眼科
·
药物递送系统
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
·
2022
·
IF 11.322
Caili Lv,Xiaosong Zhou,Peng Wang,Jianchao Li,Zhenxu Wu,Zixue Jiao,Min Guo,Zongliang Wang,Yu Wang,Liqiang Wang,Peibiao Zhang
Massive bleeding from deep penetrating wounds and bacterial infections are serious challenges for clinical and outdoor wound management. In this study, different molar ratios of chitosan (CS) and dopamine (DA) were covalently combined with sodium alginate (SA) through EDC/NHS to form complexes. Then, the cross-linking reaction was performed to form a sponge by adding sodium periodate. The CS 3 /SA/DA 1 compress sponge has rapid blood-triggered recovery properties that can quickly expand to block wounds and prevent bleeding. The CS 3 /SA/DA 1 has better coagulation ability than commercial gelatin sponges and gauze in a mouse liver injury/leg vein model and lethal incompressible hemorrhage model of a rabbit liver defect. The outstanding advantages of CS 3 /SA/DA 1 in degradation performance , antibacterial properties , antioxidation, hemocompatibility , cytocompatibility and histocompatibility present great application prospects in incompressible wound hemostasis.
研究领域:
生物材料
·
伤口愈合
·
止血
·
抗菌材料
·
组织工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）