Food Analytical Methods
·
2023
·Vol. 16
·期 5
·909-917
·
IF 2.9
Xu Yanfen,Lu Ying,Yu Jianna,Liu Wen,Jing Guoxing,Li Wenshan,Liu Wenjie
Tuna is one of the most popular fish and therefore among the most commercially valuable seafood. However, inadequate refrigeration during the whole supply chain causes serious economic loss and food poisoning. One important result of tuna spoilage is the accumulation of biogenic amines such as histamine, which can be used as a sensitive indicator for Tuna freshness. Thus, the development of rapid, sensitive, and economic analytical methods for biogenic amines in Tuna is highly desired. This research developed a method that combines high-performance liquid chromatography (HPLC) and electrospray ionization ion mobility spectrometry (ESI-IMS) to detect 7 kinds of biogenic amines including tryptamine, tyramine, spermidine, cadaverine, β-phenylethylamine, putrescine, and histamine in tuna. The limit of detections (LODs) for 7 biogenic amines are 0.01, 0.02, 0.02, 0.17, 0.03, 0.04, and 0.15 mg L −1 respectively. The linear range of histamine and spermidine is 2–100 mg kg −1 , and the linear range of other biogenic amines is 0.5–10 mg L −1 . The recoveries of biogenic amines are between 69.39 and 112.65%. Compared to the traditional HPLC-based analytical method, the proposed method does not involve any derivatization steps, which greatly decreased the sample preparation time and cost. Another advantage of the developed method is the supplementary separation to the HPLC, in which coeluted biogenic amines can clearly be separated in the ion mobility dimension. The proposed method is applied to determining the concentration of biogenic amines in tuna storage, which showed that this method can quickly and accurately monitor the changes of biogenic amines in tuna during storage.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-9
·
IF 5.479
Chen Jia,Li Yunchun,Huang Yanni,Zhang Haijuan,Chen Xingguo,Qiu Hongdeng
The authors describe a fluorometric strategy for the determination of dopamine (DA). It is based on the use of aptamer-functionalized MoS 2 quantum dots (QDs) and MoS 2 nanosheets (NSs). The QDs and NSs were extensively characterized with regard to their physical and chemical properties using methods such as TEM, XRD, FT-IR, EDX and molecular spectroscopies. The aptamer against dopamine was labeled with QDs acting as the energy donor in an energy transfer system, while the NSs serve as the energy acceptor. Under the optimal conditions, the fluorescence (FL) intensity (best measured at excitation/emission peaks of 315/412 nm) increases with increasing DA concentration in the range from 0.1 nM to 1000 nM, with a lower detection limit of 45 pM. The method was successfully applied to the determination of DA in complex matrices. In our perception, the method has a wide scope in that it may be extended to other biomolecules for which respective aptamer are available. The QDs show excellent optical properties, good stability, low cytotoxicity, and may also be applied to fluorometric imaging of live cells.
A “turn-on” fluorometric aptasensor for the determination of dopamine (DA) was established based on aptamer-functionalized molybdenum disulfide quantum dots (MoS 2 QDs) and MoS 2 nanosheets. This assay exhibits high selectivity and sensitivity with a detection limit as low as 45 pM.
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POLYMER ENGINEERING AND SCIENCE
·
2019
·Vol. 59
·期 10
·2008-2015
·
IF 1.92
Rui Chen,Shensong Dai,Tong Guo,Hao Tang,Yuqi Fan,Hong Zhou
A novel flame-retardant hsaltryptamine phosphaphenanthrene (HTD) was successfully synthesized and characterized by Fourier transform infrared spectrometry and nuclear magnetic resonance. HTD was used to improve flame retardancy of epoxy resin (EP). When the addition content of HTD reached 4 wt%, the EP/HTD thermoset passed a UL-94 V-1 rating and had limited oxygen index value of 29.2%. Compared to neat EP, the release of heat and smoke decreased significantly due to the gaseous-phase and condensed-phase flame-retardant mechanism of HTD. The char residue after cone calorimeter test was analyzed and the results showed that the char residue of EP/8%HTD thermoset exhibited homogeneously crosslinked char layer, which acted as a good barrier for the release of heat and oxygen. The transmittance and mechanical properties of EP/HTD thermosets had been tested. EP/HTD thermoset possessed high transparency and good mechanical properties. In comparison to neat EP, tensile strength, flexural strength, and Izod unnotched impact strength of EP with 8% loading of HTD was improved by 21.9%, 20.5%, and 17.4%, respectively. POLYM. ENG. SCI., 59:2008–2015, 2019. © 2019 Society of Plastics Engineers
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-9
·
IF 5.705
Wang Zhong-Xia,Gao Yuan-Fei,Yu Xian-He,Kong Fen-Ying,Lv Wei-Xin,Wang Wei
The authors describe the synthesis of fluorescent coral-like carbon nano-branched polymers (PCNBPs) co-doped with nitrogen and phosphorus. Uric acid and phosphoric acid act as nitrogen and phosphorus sources, respectively. The PCNBPs have a coral-like branched structure, are cross-connected, and < 20 nm in skeleton diameter. Their blue fluorescence, best measured at excitation/emission wavelengths of 330/425 nm, is quenched by mercury (II) ions due to the specifically restricted rigid conformation caused by the interaction of phosphorus, nitrogen, and oxygen groups on the surface of the PCNBPs. Fluorescence is selectivity quenched by Hg(II) but restored in addition of the hypertension drug captopril (CAP) in the range 50 nM to 40 μM concentration range. Fluorescence recovery is attributed to the effectively specific interactions between the thiol group of CAP and Hg(II). The method was applied to the determination of the concentration of Cap in pharmaceutical samples, and recoveries were between 97.6 and 105.1%.
Fluorescent coral-like carbon nano-branched polymers (PCNBPs) co-doped with nitrogen and phosphorus are described. Their fluorescence is selectivity quenched by Hg(II) but restored in addition of the hypertension drug captopril (Cap) in the range 50 nM to 40 μM concentration range.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Qi-Ci Wu,Qi-Lei Zhang,Dong Gao,Lei Nie,Hai-Bin Wang,Shan-Jing Yao,Dong-Qiang Lin
Mixed-mode chromatography (MMC) is a promising technology for protein separation with high adsorption capacity, good selectivity, salt-tolerance, and facile elution. Expanded bed adsorption (EBA) can capture target proteins directly from bioparticle containing crude feedstock without solid–liquid separation. In this study, MMC and EBA were combined to develop a mixed-mode EBA technique for human serum albumin (HSA) separation. Two mixed-mode EBA resins (using quartz-densified agarose beads and tungsten carbide/agarose composite beads, respectively) with tryptamine as the ligand were prepared (TA-S and TA-T). Static and dynamic adsorption behaviors of HSA were determined under varying conditions and typical adsorption properties of pH dependence and salt-tolerance were found. The performance of the resins in expanded bed was studied, and the dynamic binding capacities at 10% breakthrough (Q10%) of TA-S and TA-T at bed expansion of 1.8 were 27.54 and 18.04 mg/mL settled resin, respectively. Moreover, these two resins were used to separate recombinant HSA (rHSA) from Pichia pastoris culture broth. TA-S showed better separation performance and the purity of rHSA monomer reached to 83.3% with the recovery of 91.6% through a two-step elution process. Total content of rHSA monomer and degraded fragment was 99.8%. The results demonstrate that the combination of MMC and EBA would be a potential platform for protein capture with fewer pretreatments and high process efficiency.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yancheng Xie,Xue Feng,Jiaqi Tao,Qianhui Gao,Yue Li,Xinyi Liu,Mingyu Xia,Dong Wang
5-Hydroxytryptamine (5-HT) and its derivative bufotenine, which possess important physiological functions, are the primary active components in the secretions of toad parotid and skin gland. However, the biosynthetic pathway of these substances remains unclear in toads. To characterize toad's Aromatic-L-amino-acid decarboxylase (AADC), the key enzyme in the predicted 5-HT derivatives biosynthetic pathway, the full-length cDNA of AADC from Bufo bufo gargarizans ( Bbg AADC) was cloned from the parotoid gland of B. bufo gargarizans . The recombinant Bbg AADC exhibited optimal expression in E. coli BL21 (DE3) containing pCold- Bbg AADC after induction for 16 h at 15 °C with 0.3 mM IPTG, resulting in substantial yields of soluble proteins . The enzymological properties of Bbg AADC were assessed, and it was determined that the optimal reaction temperature was 37 °C, the optimal pH was 8.6, and the optimum molar ratio of pyridoxal-5′-phosphate (PLP) to Bbg AADC was found to be 3.6:1. Additionally, high substrate specificity was observed, as Bbg AADC could catalyze the production of 5-HT from 5-hydroxytryptophan (5-HTP) but not dopamine or tryptamine from levodopa or tryptophan , respectively. The Km of the recombinant protein Bbg AADC was 0.2918 mM and the maximum reaction rate (Vmax) was 1.182 μM·min −1 when 5-HTP was used as substrate. The Kcat was 0.0545 min −1 , and Kcat/Km was 0.1868 mM −1 ·min −1 . To elucidate the mechanism of Bbg AADC, molecular docking was performed with PLP and 5-HTP, or the external aldimine formed by 5-HTP and PLP. The results indicated that the active sites for Bbg AADC to bind with PLP were K303, H192, N300, A148, F309, T246, A273, and T147. W71, Y79, F80, P81, T82, H192, T246, N300, H302, F309, and R477 served as catalytically active sites for the binding of Bbg AADC to 5-HTP. Furthermore, R447, W71, S149, N300, A148, and T147 of Bbg AADC were involved in the decarboxylation reaction of the aldimine formed by PLP and 5-HTP.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yanan Liu,Jiacheng Gan,Fei Chen,Jian Chen,Huan Li,Yanbo Wang
Dual-enzyme co-embedded materials have shown high potential for achieving efficient detection due to the convenience of two-enzyme cascade reactions. Herein, we developed a dual-enzyme hybrid microsphere (HM) based biosensor to detect diamines (histamine was included for ease of description) in aquatic products. The HM was made from diamine oxidase , horseradish peroxidase , and copper phosphate through the biomineralization method. Under optimal conditions, the system displayed linear color response to histamine of different concentrations ranging from 0 to 200 μg/mL. The detection limit of histamine was 0.15 μg/mL, showing higher sensitivity than the two-step free enzyme assay . Moreover, the detection system exhibited good specificity to diamines. The method was used to detect diamines in commercial samples, and the results were compared with those measured by the high-performance liquid chromatography method. Overall, the proposed assay exhibited high potential in diamine quantification and was readily extended to other cascade enzymatic reaction-based detection strategies.
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FOOD CONTROL
·
2024
·
IF 5.6
Xia Xu,Xin Liu,Yixing Qiu,Yufan Song,Xuxia Zhou,Yuting Ding
The accurate determination of histamine in fish is imperative for food safety, given the toxic nature of this biogenic amine . This study presents an innovative colorimetric aptasensor for the ultrasensitive detection of histamine in fish, utilizing nanozymes based on magnetic metal-organic frameworks coated with Pt nanoparticles (Fe 3 O 4 @MOF@Pt), which exhibit high peroxidase-like catalytic activity, magnetism, and superior optical properties. The surface of Fe 3 O 4 @MOF@Pt nanozymes were modified with DNA strands complementary to the histamine aptamer , creating effective colorimetric probes. In the presence of histamine, these colorimetric probes compete with histamine molecules for aptamer binding on the enzyme plate. Color development is triggered by adding a TMB and H 2 O 2 substrate solution to the enzyme plate. This competitive binding-based aptasensor allows for rapid and accurate histamine quantification within a linear range of 10 pM to 10 μM, and a notable detection limit of 6.23 pM. Furthermore, the sensor demonstrated excellent specificity, distinguishing histamine from l -histidine and other biogenic amines. Tested on mackerel samples, the aptasensor showed spiked recoveries of 91.4%–109.6%, validating its practical applicability. This sensor offers an ultrasensitive, specific, and reusable tool for histamine detection, representing a significant advancement in ensuring the safety of fish products.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Chengtuo Niu,Yiyang Liu,Heng Li,Chunfeng Liu,Qi Li
This study aimed to elaborate the biochemical and chemosensory characteristics of doubanjiang fermentation via a two-stage controlled temperature. HL group with variable temperature (40 °C → 30 °C) and NTF group fermented following traditional technique were prepared and their volatile and non-volatile metabolites were compared through multivariate statistical analysis. HL group favored the accumulation of amino acid nitrogen, free amino acids and organic acids in the early stage and maintained adequate total acids and biogenic amines in the mid-late stage. HL group also had preferred jiang and fruity flavor through sensory evaluation. A total of 116 volatile metabolites were identified in HL or NTF groups and 22 marker volatile metabolites were screened through the combinational use of OPLS-DA and Random Forest analysis. Stronger anti-oxidant ability was observed in HL group while adequate number of acidic compounds and biogenic amines were ensured. This indicated that the two-stage controlled temperature fermentation was beneficial for doubanjiang fermentation.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2017
·
IF 1.174
Qici Wu,Qilei Zhang,Shiwen Xu,Chengtong Ge,Shanjing Yao,Dongqiang Lin
Five tryptophan analogues with a hydrophobic indole ring and an amino group on each molecule were used as functional ligands of mixed-mode resins for human serum albumin (HSA) purification. Their adsorption performance was evaluated and the effects of pH and salt addition on HSA adsorption were studied. The resins prepared showed typical pH-dependent adsorption and the highest adsorption capacity and affinity were found at pH 5.0 for all the resins tested. The saturated adsorption capacity was 138.02 mg·g − 1 with the tryptamine-functionalized resin, which significantly decreased at pH below 4.0 due to electrostatic repulsion between ligands and HSA. Moreover, the addition of NaCl or (NH 4 ) 2 SO 4 in media reduced HSA adsorption capacity, although the two salts showed different affecting profiles. The tryptamine-functionalized resin showed the best salt-tolerant performance, and its high adsorption capacity was maintained under high salt concentrations. In addition, the five resins prepared showed good adsorption selectivity for recombinant HSA from Pichia pastoris broth. Molecular docking results between tryptamine and HSA indicated that tryptamine was favorable to bind on Site II (indole-binding site) of HSA.
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JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Qi-Ci Wu,Dong-Qiang Lin,Wei Shi,Qi-Lei Zhang,Shan-Jing Yao
Mixed-mode chromatography (MMC) is a new technology that uses specially-designed ligands to improve the adsorption selectivity with multimodal protein–ligand interactions for protein separation. A new MMC resin TA-B-6FF with tryptamine as the functional ligand was prepared and used for human serum albumin (HSA) separation. Adsorption equilibria of plasma-derived HSA (pHSA) were investigated and compared with a commercial tryptophan-based resin (MX-Trp-650m), and the influence of pH and salt addition was studied. The results showed that weak acidic conditions (pH 5.0–7.0) were favorable for HSA adsorption. The maximum adsorption capacity of TA-B-6FF was 141.33 mg/g at pH 5.0, which was two times higher than that of MX-Trp-650m. TA-B-6FF also showed better salt-tolerance than MX-Trp-650m. Moreover, TA-B-6FF was used to separate recombinant HSA (rHSA) from Pichia pastoris culture broth. The results indicated that rHSA could be directly captured by TA-B-6FF without dilution or pH adjustment. High purity (87.75%) of rHSA monomer could be obtained with a recovery of 98.53% through two-step elution process. Total content of rHSA monomer and degraded fragment was 99.75%, the removal of host cell proteins reached about 90%. The results demonstrate that new TA-B-6FF resin has a great potential for rHSA purification directly from the complex fermentation broth.
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ENVIRONMENTAL POLLUTION
·
2016
·
IF 4.839
Dezhen Wang,Xinru Wang,Ping Zhang,Yao Wang,Renke Zhang,Jin Yan,Zhiqiang Zhou,Wentao Zhu
Epidemiological studies indicate that exposure to persistent organic pollutants is positively associated with the prevalence of obesity. To delineate the potential role of technical-grade chlordane in obesity development, chlordane metabolism and chlordane-induced metabolic changes were investigated in mice fed high-fat diet (HFD) over a 6-week period. Gas chromatography–electron capture detector analysis showed that HFD induced more accumulation of technical chlordane in the liver, muscle and adipose tissue . The enantioselectivities of oxychlordane in selected tissues were also influenced by HFD. 1 H NMR-based liver metabolome indicated that technical chlordane can enhance the metabolic alterations induced by HFD. Compared with the low-fat diet (LFD) group, no differences were observed in the LFD + chlordane group. However, as many as 16 metabolites were significantly different between the HFD group and HFD + chlordane group. Moreover, compared to the LFD + chlordane group, the abundances of 24 metabolites significantly increased or decreased in the HFD + chlordane group. Twenty metabolites were altered in the HFD group compared to the LFD group. Tryptophan profiling suggested that both chlordane and HFD can disturb tryptophan catabolism. These interactions between technical chlordane and HFD suggest that technical chlordane is a candidate obesogen.
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