ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yi-Fei Xue,Xiaojie Song,Xiao-Qing Ling,Qi-Yan Lv,Yu Xia,Hui-Fang Cui
Nanoassemblies based on drug conjugates with high drug loading efficiency and stability have been regarded as promising candidates for the next generation of drug formulations. However, they are mostly amphiphilic. Here, a dual-hydrophobic drug conjugate-based nanoassembly has been created for enhanced synergistic antiproliferation against colorectal cancer cells. Camptothecin (CPT) and doxorubicin (DOX) were chosen as the hydrophobic drugs and covalently linked with a disulfide bond (-ss-). The synthesized CPT-ss-DOX can self-assemble into nanocubes (NCs) in an aqueous solution with the assistance of a small amount of polyethylene glycol (PEG), named PEGylated CPT-ss-DOX NCs. The PEGylated CPT-ss-DOX NCs were approximately 111.8 nm, possessing a crystal structure and a very low critical aggregation concentration (8.36 μg·mL–1). The self-assembly mechanism was studied using molecular docking and molecular dynamic simulation methods. The NCs demonstrated excellent storage stability and improved water solubility of CPT and DOX. These NCs could be taken up by cancer cells and gradually release the drugs. In addition, they had higher toxicity to cancer cells than a mixture of CPT and DOX, while they displayed reduced toxicity to normal cells. Due to assembly and PEG modification, the NCs improved drug retention time and enhanced accumulation at the tumor site. More importantly, they significantly inhibited colorectal tumor growth (58.37%) in vivo, superior to the CPT+DOX mix (42.63%). Moreover, the NCs reduced the cardiac toxicity of free drugs. Therefore, the prepared PEGylated CPT-ss-DOX NCs hold great potential for clinical transformation and provide a novel method for the self-delivery of hydrophobic molecules in cancer therapy.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Guannan Liu,Sijin Wu,Shijie Fang,Yi Wang,Muhammad Bilal,Shujun Shen,Chong Xie,Pei Wang,Runqiang Yang
We have previously reported that soybean germination increases the water-holding capacity (WHC) of glucono-δ-lactone (GDL)-induced soymilk gels while weakening their gel strength. However, the associated mechanism(s) underlying this decrease in gel strength remains unclear. Herein, we investigated the mechanisms underlying the effects of germination on soymilk gel formation. To this end, we prepared GDL-induced soybean protein isolate (SPI) gels. Germination-induced changes in the SPI structure and thermal aggregation behavior were examined. Subsequently, the gelation properties of the SPIs derived from germinated soybeans were investigated, including texture, WHC, microstructure, rheology, thermogravimetry, and intermolecular forces. Germination induced the production of small-molecule proteins and peptides in SPI. Additionally, the SPI structure unfolded, exposing hydrophobic groups on the surface and causing the protein structure to become increasingly disordered with increased germination time. Meanwhile, most of the newly formed small peptides (< 1.3 kDa) produced during germination did not contribute to gel formation. Mechanistically, as the hydrophobic interactions increased, the disulfide bonds decreased during protein pre-aggregation and gel formation. This interfered with the gel’s three-dimensional network structure development, decreasing SPI gel hardness, viscoelasticity, and thermal stability while increasing the WHC. These findings offer insights into the protein-level gelling mechanism of soymilk gels created with germinated soybeans, inform the development of innovative gelation technology, and advance the industrial development of germinated soybeans.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-11
·
IF 5.3
Gu Yingqiu,Fang Puhao,Chen Yu,Xie Tianhua,Yang Guohai,Qu Lulu
A miniature multi-channel surface-enhanced Raman scattering (SERS) sensor based on polydimethylsiloxane (PDMS) is constructed to achieve rapid delivery of polluted water and specific identification of multiple components. Hg 2+ , organic pollutants, and sodium nitrite are successfully identified by the multi-channel SERS sensor using Cy5, cyclodextrin, and urea in the corresponding detection area. This multi-channel sensor exhibits excellent sensitivity and specificity, with detection limits of 3.2 × 10 −10 M for Hg 2+ , 1.0 × 10 −8 M for aniline, 6.9 × 10 −9 M for diphenylamine, 9.1 × 10 −8 M for PCB-77, and 7.5 × 10 −9 M for pyrene, and 5.0 × 10 −7 M for sodium nitrite. Compared with traditional analysis techniques, this method exhibited excellent recovery for the water pollutants ranging from 82.1 to 115.8%. The PDMS-based microchannel allows for simultaneous and rapid identification of multiple environmental pollutants, offering a portable detection method for emergency testing of environmental pollutants and routine determination of water pollutants. Graphical Abstract
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MOLECULAR PHARMACEUTICS
·
2026
·
IF 4.9
Ning Yang,Yule Song,Junliang Song,Jian Qin,Mingwei Fu,Feng Yao,Huan Xu
Renal fibrosis (RF), a critical pathogenic mechanism in chronic kidney disease (CKD), now has exceedingly few therapeutic alternatives. This study presents a new sigma1 receptor (σ1R)-targeted nanodelivery system utilizing the natural polyphenolic medicine resveratrol (Res) as a therapeutic agent, addressing its low bioavailability and inadequate targeting properties, thereby providing a significant advancement in the treatment of RF. We engineered and produced a multifunctional material, aminoethyl anisamide-poly(2-ethyl-2-oxazoline)-cholesterol hemisuccinate (APC)-with benefits including active targeting, prolonged circulation, and pH sensitivity. In vitro investigations demonstrated that in the high glucose-induced HK-2 cell fibrosis model, the absorption efficiency of APC-modified liposomes was markedly enhanced, effectively suppressing the expression of fibrotic and inflammatory factors. In two typical animal models of RF, namely diabetic nephropathy and glomerulonephritis, APC@Res demonstrated therapeutic advantages, including modulation of the TGF-β1/Smad3 signaling pathway to impede fibrosis, a notable reduction in oxidative stress levels, enhanced renal function parameters, and diminished expression of inflammatory and fibrotic markers. This study innovatively employs the renal microacidic environment and sigma1 targeting to develop an effective delivery system, offering a novel treatment strategy for reversing RF.
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Communications Materials
·
2026
·
IF 9.1
Ma Wei,Zhang Jin-Yan,Wang Dun,Li Zhi-Cheng,Cheng Xiang-Yi,Chen Ling-Long,Liu Ying,Kang Gui-Ying,Yu Cui-Yun,Wei Hua
The leverage of synthetic polymer self-assemblies (SPSAs) as biomimetic allostery provides unique opportunities for developing bio-inspired soft species. However, the preparation of these soft species remains a challenge likely due to the synthetic difficulties toward SPSAs having four-level hierarchical structural transitions and the compromised colloidal stability of the self-assembled quaternary structures. Herein, we report self-assembly of topological polymers with hydrophilic multicycles (HMCs) for biomimetic allostery. Specifically, cationic copolymers containing HMCs are prepared via a reliable synthetic approach, whose hierarchical self-assembly affords stabilized nanoparticles that undergo biomimetic allostery into quaternary petalled self-assemblies at an intracellular acidic pH of 5.0. The quaternary petalled self-assemblies lead to a mechanical disruption effect on lysosomes like nanomachines, further resulting in enhanced drug delivery efficiency of an anticancer drug-loaded multicyclic copolymers with a tumor inhibition rate 1.6-fold greater than that of the free drug in a murine 4T1 model. Overall, our work highlights the use of HMCs as biomimetic allostery of SPSAs for enhanced biomedical applications.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Wang Weilin,Hu Qiaoying,Wu Chunyan,Ding Jiayue,Zhu Chuanxiu,Wen Jiayao,Li Yingchao,Yang Menghan,Wang Yujie,Sun Deqing,Zhai Guangxi,Zhang Yanan
Ferroptosis and sonodynamic therapy (SDT) have both been recognized as powerful weapons in cancer treatment, especially in non-small-cell lung carcinoma (NSCLC) recently. However, the lack of effective sono-sensitizer and ferroptosis regulator limited their usage. In order to overcome the limitation, a novel nanoplatform of DTX-CS/ART/PFCA@RGD (cRCAPD) was fabricated based on SDT and ferroptosis. Nanoparticles, under the action of c(RGDfk) cyclic peptides and their skeleton chondroitin sulfate (CS), could specifically target αvβ3 and CD44 receptors, respectively, thereby targeting tumor sites. Meanwhile, the peroxy-bridge structure of ART disrupted the intracellular iron homeostasis while generating highly toxic reactive oxygen species (ROS) under the SDT. Docetaxel (DTX) in nanoparticles led to the excellent apoptosis of A549 cells. Oxygen carried by PFCA alleviated the tumor hypoxic microenvironment, downregulated HIF-1α, and provided substrates for SDT. Further, the nanoparticles showed a strong immunogenic cell death (ICD) effect, modulating the number of T cells and dendritic cells in tumor-bearing mice. In summary, the proposed therapeutic strategy based on SDT and ferroptosis holds promising potential for synergistic treatment of lung cancer in future clinical applications. Graphical The alternative text for this image may have been generated using AI.
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Ping Wang,Meijuan Xue,Xinhui Guan,Xiaofei Gu,Xiaoqiang Qiao
Background Phospholipids in serum exosomes are promising biomarkers for breast cancer diagnosis. However, the separation of phospholipids by liquid chromatography presents several challenges due to their inherent structural heterogeneity and the presence of isomeric forms. Traditional columns often struggle with the overlapping retention times of phospholipid species, which can result in poor resolution and difficulty in accurate quantification. Furthermore, the varying polarities and hydrophobicities of different phospholipid classes necessitate specialized stationary phases to achieve optimal separation. This study aimed to develop a novel chromatographic material for the comprehensive analysis of phospholipids in complex biological matrices. Results In this study, a novel silica-based Sil-SMA-GMS copolymer brush stationary phase was fabricated for the first time via reversible addition-fragmentation chain transfer (RAFT) polymerization, using styrene-maleic anhydride (SMA) copolymer as the functional matrix and glycerol monostearate (GMS) as the derivatization reagent. Benefiting from the synergistic effects of hydrophobic moieties (benzene rings and long alkyl chains) and hydrophilic groups (carboxyl and hydroxyl groups) in the SMA copolymer, the prepared stationary phase exhibited a unique mixed hydrophilic interaction chromatography/reversed-phase liquid chromatography (HILIC/RPLC) retention mechanism. It demonstrated excellent separation selectivity and stability for both hydrophilic and hydrophobic small molecules, achieving a maximum column efficiency of 81,029 N/m. Notably, this stationary phase overcame the limitations of traditional single-mode chromatographic columns, enabling the simultaneous separation and analysis of phospholipids that vary in class, acyl chain length, and degree of unsaturation. Furthermore, by coupling the Sil-SMA-GMS column with mass spectrometry (MS), a non-targeted analytical method for serum exosomal phospholipids was developed. Applying this method to serum exosomes from breast cancer patients and healthy controls led to the identification of 9 major classes encompassing 200 phospholipid molecular subtypes, as well as the screening of phospholipid markers closely associated with breast cancer pathogenesis. Significance This work presents a novel chromatographic material for the efficient separation of phospholipids in complex biological samples and establishes a technical foundation for the construction of multidimensional lipidomics platform. This advancement provides crucial support for the screening of cancer-related biomarkers, elucidating tumor metabolic mechanisms, and exploring targeted therapeutic targets.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2026
·
IF 5.6
An Chang,Yi Wang,Zan Cui,Yuan-Yuan Liu,Ling-Qi Meng,Jia-Shi,Zhi-Yong Qin,Jia-Ning Gong
Pulmonary fibrosis (PF) is a progressive and lethal interstitial lung disease, characterized by excessive extracellular matrix deposition and architectural distortion of the lung parenchyma. Its pathogenesis involves interconnected pathological events, including dysregulated epithelial-mesenchymal transition (EMT), chronic inflammation driven by M2-polarized macrophages, and abnormal fibroblast activation. These intertwined mechanisms contribute to the limited efficacy of current anti-fibrotic therapies, which often fail to achieve lesion-specific targeting and disease reversal. Moreover, the clinical utility of existing drugs is further hampered by poor bioavailability and insufficient accumulation at fibrotic sites. To overcome these challenges, we developed a multifunctional nanotherapeutic platform, termed HPA@NPs, through co-assembly of hyaluronic acid-platycodin D (HA-PD) and aspirin-platycodin D (ASA-PD) conjugates. This nanosystem enables concurrent modulation of multiple PF-relevant pathological features. Physicochemical characterization showed that HPA@NPs possess uniform nanoscale size, low critical aggregation concentration, and excellent colloidal stability, supporting prolonged blood circulation. In vitro, HPA@NPs showed efficient cellular uptake consistent with their HA-based design and were accompanied by inhibition of EMT, fibroblast activation, and M2 macrophage polarization, together with reduced expression of IL-10 and Arg-1. In bleomycin-induced PF mice, HPA@NPs significantly improved pulmonary function and attenuated histopathological damage. At the molecular level, HPA@NPs down-regulated fibrosis-related markers (Col1a1, TGF-β1, α-SMA), while up-regulating the epithelial tight-junction protein ZO-1 and down-regulating mesenchymal markers (N-cadherin, vimentin), confirming effective reversal of EMT. The therapeutic outcome of HPA@NPs surpassed that of monotherapy, highlighting a synergistic anti-fibrotic effect. Importantly, HPA@NPs exhibited no detectable cytotoxicity, hemolytic activity, or major organ toxicity at therapeutic doses, demonstrating favorable biocompatibility. In summary, HPA@NPs integrate precise targeting, multi-pathway synergy, and excellent biosafety, offering a promising translational strategy for PF treatment.
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LANGMUIR
·
2026
·
IF 3.9
Yunxing Li,Huiqin Zhan,Chen Li,Jie Mei,Hang Jiang,Man-Hin Kwok,To Ngai
Pickering double emulsions have attracted increasing interest due to their potential applications in food, pharmaceuticals, and cosmetics. Currently, the preparation of Pickering double emulsions relies heavily on surface treatments of particle stabilizers, particularly in the one-step emulsification approach. This strategy is inconvenient and may result in limitations that hinder practical applications. Here, we demonstrate that oppositely charged starch nanocrystals and chitin nanocrystals can be used simultaneously to form Pickering double emulsions by using a one-step emulsification procedure that is independent of surface modifications. Starch nanocrystals and chitin nanocrystals exhibit a synergistic effect in stabilizing both the internal and the external droplets. Using both natural nanocrystals together produces Pickering double emulsions with improved stability, which is attributed to the adsorption of binary colloid complexes formed by the two nanocrystals at the oil/water interface of opposing curvatures. The electrostatic interactions between the nanocrystals drive the formation of binary colloid complexes. The starch/chitin mass ratio, aqueous pH, and salt concentration all influence the hydrodynamic size, ζ-potential, and surface wettability of the resulting complexes prior to emulsification, which collectively govern double emulsion formation and stability. As an illustration, the prepared Pickering double emulsion effectively protects the lipophilic β-carotene and hydrophilic riboflavin sodium phosphate simultaneously under different conditions.
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ChemistrySelect
·
2023
·Vol. 8
·期 1
·e202204013
·
IF 2.1
Yanrong Li,Haohao Li,Yang Xu
Graphical In this work, we proposed a kind of N-CDs which can specially respond to Mn 2+ with a minimum concentration of 5 nM. Notably, N-CDs-based visible warming of Mn 2+ over drinking water standard can be achieved. Manganese is one of vital elements related to various diseases. Nowadays, few works can specifically respond to Mn 2+ alone. Hence, it is still challenging for specific detection of Mn ion. In this work, nitrogen-doped carbon dots (N-CDs) with blue luminescence properties was prepared using nitrated pyrene and diethylenetriamine (DETA) via one-step solvothermal process. N-CDs were applied as a fluorescent probe for specific detection of Mn 2+ because of their high selectivity and sensitivity. No apparent fluorescence interferences can be observed with various amino acids and metal ions. The minimum concentration detected was 5 nM. The quenching mechanism maybe attributed to dynamic quenching processes. Notably, in this work, a visual early-warning of Mn content exceeding the standard in drinking water in the light of fluorescence quenching of N-CDs was achieved, and the practical sensing of N-CDs which performed in environmental water samples demonstrated the detection performance of N-CDs in real samples.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-10
·
IF 6.408
Sun Min,Wang Xiuqin,Ding Yali,Feng Juanjuan
In order to improve the selectivity and stability of melamine–formaldehyde (MF) aerogel, it was composited with TiO 2 aerogel. A TiO 2 -MF hybrid aerogel was in situ prepared on the surface of carbon fibers for in-tube solid-phase microextraction (SPME). The extraction performance of TiO 2 -MF aerogel was regulated by changing the ratio of TiO 2 sol and MF sol during the material preparation. Coupled with high-performance liquid chromatography-diode array detection (HPLC–DAD), the extraction tube filled by TiO 2 -MF aerogel-coated carbon fibers was evaluated with several types of environmental pollutants including polycyclic aromatic hydrocarbons (PAHs), estrogens, and ultraviolet filters. Because of favourable extraction performance of PAHs they were selected as model analytes, and some important influence factors were optimized for satisfactory sensitivity. The detection limits were in the range 0.05–0.10 μg L −1 , owing to high enrichment factors (653–1007). The online in-tube SPME-HPLC–DAD method was verified for the determination of trace PAHs in environmental water samples, and acceptable recovery (70–118%) was achieved. The analytical methods also displayed some advantages in comparison with other reports. Moreover, the extraction tube exhibited satisfactory chemical stability. Graphical abstract
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Xinze Wu,Qiqi Chu,Qiong Ma,Huaixia Chen,Xueping Dang,Xiaolan Liu
The contamination of polycyclic aromatic hydrocarbons (PAHs) in edible oil is a health threat. Thus, trace analysis of PAHs is of high necessity. Based on the efficient adsorption of PAHs on Zn 5 metal cluster, a Zn 5 functionalized copolymer monolithic column was rationally designed for pipette tip micro-solid phase extraction (μ-SPE). The modified Zn 5 improved the adsorption selectivity and capacity of the monolith for naphthalene, phenanthrene, fluoranthene and pyrene. Chemical doping and copolymerization stabilized the monolith with a long life. Under optimal extraction conditions, a μ-SPE-high performance liquid chromatography with ultraviolet detector method was established for the detection of 4 PAHs in edible oils. The method yielded detection ranges of 0.15–250 μg L −1 ( R 2 > 0.997), detection limits of 0.050–1.5 μg L −1 , satisfactory recoveries (86.3–101.5 %), and high precisions (RSDs < 7.9 %). The results indicated that the Zn 5 functionalized copolymer monolithic column was an ideal separation medium for the detection of PAHs residues in edible oils.
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