SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Shanshan Sun,Haizhen Wang,Binxin Fu,Hao Zhang,Jun Lou,Laosheng Wu,Jianming Xu
Rhodococcus sp. WB9, a strain isolated from polycyclic aromatic hydrocarbons contaminated soil, degraded phenanthrene (PHE, 100 mg L −1 ) completely within 4 days. 18 metabolites were identified during PHE degradation, including 5 different hydroxyphenanthrene compounds resulted from multiple routes of initial monooxygenase attack. Initial dioxygenation dominantly occurred on 3,4-C positions, followed by meta -cleavage to form 1-hydroxy-2-naphthoic acid (1H2N). More than 95.2% of 1H2N was transported to and kept in extracellular solution without further degradation. However, intracellular 1H2N was converted to 1,2-naphthalenediol that was branched to produce salicylate and phthalate. Furthermore, 131 genes in strain WB9 genome were related to aromatic hydrocarbons catabolism, including the gene coding for salicylate 1-monooxygenase that catalyzed the oxidation of 1H2N to 1,2-naphthalenediol, and complete gene sets for the transformation of salicylate and phthalate toward tricarboxylic acid (TCA) cycle. Metabolic and genomic analyses reveal that strain WB9 has the ability to metabolize intracellular 1H2N to TCA cycle intermediates, but the extracellular 1H2N can’t enter the cells, restricting 1H2N bioavailability and PHE mineralization.
研究领域:
环境微生物学
·
生物降解
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代谢组学
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基因组学
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产品关联
DOI
PubMed
高影响因子
ARCHIVES OF MICROBIOLOGY
·
2021
·Vol. 203
·期 7
·4259-4272
·
IF 2.552
Ahmad Manzoor,Ling Juan,Zhang Yanying,Sajjad Wasim,Yang Qingsong,Zhou Weiguo,Dong Junde
Polycyclic aromatic hydrocarbons (PAHs), originating from anthropogenic and natural sources, are highly concerned environmental pollutants. This study investigated the impact of two model PAHs (pyrene and phenanthrene) on bacterial community succession in the seagrass meadows sediment in a lab-scale microcosm. Halophila ovalis sediment slurry microcosms were established, one group was placed as a control, and the other two were treated with pyrene and phenanthrene. Bacterial community succession in response to respective PAHs was investigated by 16S rRNA amplicon sequencing. The results demonstrated that bacterial diversity decrease in each microcosm during the incubation process; however, the composition of bacterial communities in each microcosm was significantly different. Proteobacteria (37–89%), Firmicutes (9–41%), and Bacteroides (7–21%) were the predominant group at the phylum levels. Their abundance varies during the incubation process. Several previously reported hydrocarbon-degrading genera, such as Pseudomonas , Spinghobium , Sphingobacterium , Mycobacterium , Pseudoxanthomonas , Idiomarina , Stenotrophomonas , were detected in higher abundance in pyrene- and phenanthrene-treated microcosms. However, these genera were distinctly distributed in the pyrene and phenanthrene treatments, suggesting that certain bacterial groups favorably degrade different PAHs. Statistical analyses, such as ANOSIM and PERMANOVA, also revealed that significant differences existed among the treatments' bacterial consortia ( P < 0.05). This work showed that polycyclic aromatic hydrocarbon significantly affects bacterial community succession, and different PAHs might influence the bacterial community succession differently.
研究领域:
环境科学
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微生物学
·
生态学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Zhengwen Wei,Xiang-fei Lü,Wei Wang,Giuseppe Mele,Yifan Du,Zhen-Yi Jiang
Due to the toxicity and harmful of phenanthrene (PHE) pollutant to human health and the ecosystem, it is extremely urgent to find an accessible technique to efficiently alleviate PHE contamination risk. The Fe 3 O 4 -1.5benzhydrylamine (Fe 3 O 4 -1.5BENZHY) was successfully fabricated via the silane coupling and Schiff base substitution reaction. The Fe 3 O 4 matrix facilitated the practical recycling efficiency and the aromatic nucleus of the benzhydrylamine could form π-π interaction with PHE molecular to improve adsorption performance. The benzhydrylamine loading amount and synthesis strategy could influence the adsorption capacity of the fabricated magnetic nanocomposite to some extent. Multiple characterization techniques were utilized to assess the physical and chemical properties of the magnetic nanocomposite. The density functional theory (DFT) calculations combined with post-characterization not only revealed the π–π interaction of Fe 3 O 4 -1.5BENZHY and PHE was valid and usually existed in offset parallel stacking form, but also provided a deeper understanding of the underlying mechanism. Moreover, the stability and adsorption energy for all different configuration modes were evaluated by the LUMO-HOMO energy gap (E GAP ) and electric distribution. The Fe 3 O 4 -1.5BENZHY exhibited a homogeneous surface and PHE molecules were adsorbed in a monolayer form, its adsorption capacity (26.07 mg g −1 ) was significantly higher compared with original Fe 3 O 4 (13.28 mg g −1 ). This work helps broaden insight on the molecular binding mechanism of the adsorbent/adsorbate system and expands the modification strategies for magnetic oxide to achieve high-efficiency adsorption of hazardous polycyclic aromatic hydrocarbons.
研究领域:
环境科学
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材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Yan Hu,Zhendong Zhao,Huiwang Dai,Wenjun Zhou
Efficient, stable, and reusable composite of Co 3 O 4 /g-C 3 N 4 with abundant oxygen vacancies (OVs) were successfully constructed using a facile grinding and calcination method. The catalytic performances of OVs-enriched Co 3 O 4 /g-C 3 N 4 activated peroxymonosulfate (PMS) for the degradation of phenanthrene (PHE) and their underlying mechanisms were investigated. The catalytic activity of xCo 3 O 4 -C 3 N 4 with OVs was significantly greater than that of Co 3 O 4 -C 3 N 4 without OVs, and there was a substantial positive correlation between the degradation rate constant of PHE and the OVs content in xCo 3 O 4 -C 3 N 4 . Quenching experiments and EPR tests demonstrated that 1 O 2 is the dominant active species in the 2.0Co 3 O 4 -C 3 N 4 /PMS system, which differs from the radical-dominated activation pathway of conventional cobalt-based heterogeneous catalysts . The results indicate that the OVs introduction in the catalyst can effectively modulate the PMS activation pathway, and the proposed non-radical systems can achieve efficient degradation of PHE. In addition, the synergistic interaction between the redox cycle of Co 2+ /Co 3+ and the electron-rich O-C 3 N 4 carrier favored the degradation of PHE via the radical pathway. This study provides a new theoretical basis and reference for the facile preparation of PMS catalysts and even envision this approach may open new vistas in the application of non-radical systems in refractory organic pollutant treatment.
研究领域:
环境科学
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催化
·
污染物处理
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jinfeng Lu,Yuexian Liu,Ruili Zhang,Zhengyi Hu,Kai Xue,Biya Dong
Controversial results are reported whereby biodegradation of polycyclic aromatic hydrocarbons (PAHs) can be promoted or inhibited by biochar amendment of soil. Metabolomics was applied to analyze the metabolic profiles of amendment with biochar (BB) and biochar inoculated with functional bacteria ( Pseudomonas putida ) (BP) involved in phenanthrene (PHE) degradation. Additionally, metagenomic analysis was utilized to assess the impact of different treatments on PHE degradation by soil microorganisms. Results indicated that BB treatment decreased the PHE biodegradation of the soil indigenous bacterial consortium, but BP treatment alleviated this inhibitory effect. Metabolomics revealed the differential metabolite 9-phenanthrol was absent in the BB treatment, but was found in the control group (CK), and in the treatment inoculated with the Pseudomonas putida (Ps) and the BP treatment. Metagenomic analysis showed that biochar decreased the abundance of the cytochrome P450 monooxygenase (CYP116), which was detected in the Pseudomonas putida , thus alleviating the inhibitory effect of biochar on PHE degradation. Moreover, a noticeable delayed increase of functional gene abundance and enzymes abundance in the BB treatment was observed in the PHE degradation pathway. Our findings elucidate the mechanism of inhibition with biochar amendment and the alleviating effect of biochar inoculated with degrading bacteria.
研究领域:
环境科学
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微生物学
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土壤学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2024
·
IF 4.1
Hong-Xia Wang,Jing-Yu Zhao,Hui Wang,Huan Ma,Jian-Feng Ge,Ru Sun
Abnormal changes in the mitochondrial microenvironment can cause a variety of diseases. Consequently, based on the twisted intramolecular charge transfer (TICT) theory, three hemicyanine fluorescent probes 1a-c were designed and developed by linking the phenanthridine group with different electron donor groups for the dynamic detection of mitochondrial viscosity in this paper. They have the advantages of near-infrared emission, excellent pH stability and viscosity sensitivity. Optical experiments showed that the maximum absorption and emission wavelengths of the probes in different solvents located at 411 − 529 nm and 623 − 684 nm with large Stokes shifts of 133 – 230 nm. In addition, when the viscosity increased from 0.89 cP to 856 cP, their fluorescence intensities in glycerol enhanced by 115-fold, 83-fold and 303-fold compared with water, respectively. The fluorescence intensities of probes showed excellent fit to the Forster Hoffmann equation, and exhibited nearly OFF-ON response to viscosity. Moreover, the probe 1a with good photostability and low cytotoxicity could target mitochondria. It was verified that 1a also could detect variation in mitochondrial viscosity during autophagy by modelling starvation and drug-induced (rapamycin and nystatin) in HeLa cells. Therefore, this study provides a useful probe for the analysis of mitophagy process in HeLa cells by the viscosity variation of mitochondria.
研究领域:
细胞生物学
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医学
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生物化学
✓ 已验证
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 2
·419
·
IF 4.35
Pei Hu,Zhentao Sun,Yunwen Shen,Yiwen Pan
Hydrogen peroxide (H2O2) has been reported to be used for the illegal treatment of fishery products in order to obtain “fake” freshness. Residues of H2O2in food may be of toxicology concern. In this study, a nonenzymatic sensor was developed based on Fe@PCN-224 metal–organic frameworks wrapped by Nafion to detect H2O2concentration. The hybrid structure of Fe@PCN-224 was fabricated by incorporated free FeIIIions into the center of PCN-224, which was ultra-stable due to the strong interactions between Zr6and the carboxyl group. Scanning electron spectroscopy images exhibited that Nafion sheets crossed together on the surface of Fe@PCN-224 nanoparticles to form a hierarchical and coherent structure for efficient electron transfer. Electrochemical investigations showed that the Fe@PCN-224/Nafion/GCE possessed good linearity from 2 to 13,000 μM (including four orders of magnitude), low detection limits (0.7 μM), high stability in continuous monitoring (current remained nearly stable over 2300 s) and in long-term measurement (current decreased 3.4% for 30 days). The prepared nanohybrid modified electrode was effectively applied to H2O2detection in three different fishery products. The results were comparable to those measured using photometrical methods. The developed electrochemical method has a great potential in detecting the illegal management of fishery products with H2O2.Keywords:Fe@PCN-224;Nafion;nonenzymatic sensor;hydrogen peroxide;fishery products
研究领域:
食品安全检测
·
电化学分析
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2013
·
IF 1.722
Huiqing Lv,Xiaoping Zhang,XueZhi Chen,Zhijun Xie,Changfeng Hu,Chengping Wen,Kezhi Jiang
Background. Ficus pandurata H. (Moraceae) is widely used in traditional Chinese medicine as a healthy food condiment or a medicine for treatment of various diseases including inflammation. Objective. The purpose of the present study is to investigate the phytochemical compositions and antioxidant and anti-inflammatory activities of crude water (FPW) and ethanolic extracts (FPE) from Ficus pandurata H. Methods. Phytochemical compositions were identified by a high-performance liquid chromatography-electrospray ionization-mass spectrometry method (HPLC-ESI-MS). The antioxidant activities were evaluated by diphenylpicrylhydrazyl (DPPH) and hydroxyl radical assays, and the anti-inflammatory activities were evaluated by paw edema and levels of inflammatory mediator TNF-α and PGE2 in monosodium urate (MSU) crystal-induced rats. Results. Six compounds were identified by HPLC-MS method, and abundance of phenolics was found in FPE. The FPE showed concentration-dependent-significant scavenging of DPPH and hydroxyl radicals with IC50 values 118.4 and 192.9 μg/mL, respectively. The FPE treatment significantly inhibited the paw edema and the production of TNF-α and PGE2 in MSU crystal-induced rats. Conclusion. The FPE exerted stronger antioxidant and anti-inflammatory activities which may be attributed to its high phenolic content.
研究领域:
药理学
·
植物化学
·
传统医学
✓ 已验证
DOI
PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-16
·
IF 3.9
Zeng Liqing,Yang Hui,Tong Tong,Chen Jielun,Huang Yujie,Yang Jiaming,Xiao Yuhui,Yang Fan,Chen Yazhen
This study delved into the impact of aging on the properties of five common microplastic types, including polymethylmethacrylate (PMMA), polystyrene (PS), polyvinyl chloride (PVC), polyethylene (PE), and polypropylene (PP). The aging process significantly altered the particle size distribution: PS, PP, and PMMA underwent a contraction, with average sizes decreasing by 6.8%, 3.2%, and 1.7%, respectively, whereas PE and PVC experienced an expansion, with increases of 3.1% and 1.7%. Notably, aging generally increased the specific surface area of all microplastics by more than 20%, a change that could influence their environmental interactions. Scanning electron microscopy revealed marked surface cracks and depressions in aged PE and PVC, in contrast to minor surface alterations in PS and PMMA. Fourier transform infrared spectroscopy further indicated modifications in the characteristic peaks of aged PMMA, PP, and PE microplastics. Exposure experiments demonstrates that increasing microplastic concentrations from 100 mg/L to 5000 mg/L accelerated mortality rates in clams, with juveniles exhibiting slower mortality onset compared to adults. Prolonged exposure led to rising mortality rates across all groups, suggesting a cumulative toxic effect from long-term microplastic exposure. These findings underscore the environmental ecological risks associated with the altered physicochemical properties of aged microplastics, particularly for the Philippine clam. This study provides an essential insight for advancing our understanding of microplastic behavior and their ecological impacts, highlighting the need for further research to mitigate these environmental threats.
研究领域:
生态学
·
环境科学
·
海洋生物学
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产品关联
DOI
PubMed
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2025
·
IF 7.4
Hao Wang,Yadong Wang,Jingjing Gao,Zongmin Zhu,Fei Xiao
Herein, we presented a multifunctional epoxy resin (EP) composite prepared by the synergistic combination of DOPO and ionic liquids (ILs). The optimized EP-DOPO/ILs system demonstrated exceptional flame retardancy, with only 3 wt% of flame retardant (DOPO/ILs) added to achieve UL-94 V-0 grade for EP and a limiting oxygen index (LOI) of 33.4%. Cone calorimeter test revealed 32.2% and 26.7% reductions in peak of heat release rate (pHRR) and total smoke production (TSP), respectively, compared to neat EP. Notably, the designed rigid-flexible epoxy network simultaneously enhanced tensile strength to 75.7 MPa (+15.7%) and impact strength to 24.8 kJ m -2 (+136.3%), outperforming conventional EP thermosets. Furthermore, the EP composites retained 90% visible light transmittance while exhibiting ultraviolet (UV) shielding efficiency and intrinsic fluorescence. This multi-component collaborative system provides an important experimental foundation for developing high-performance multifunctional polymeric materials.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 114
·期 3
·1-6
·
IF 2.2
Huang Zaoquan,Li Xiaoyuan,Hou Siying,Zhao Yanjie,Lu Haijian,Wang Jun,Deng Yirong
To enhance phenanthrene degradation in soil, the effects of four surfactants on the thermal activation of sodium persulfate (SPS) were investigated. Sodium dodecyl diphenyl ether disulfonate (C 12 -MADS) was found to stabilize SPS by reducing the generation of sulfate radicals from its thermal decomposition. Optimal conditions for the surfactant-assisted persulfate system were determined to be 5 g/L of C 12 -MADS at a temperature of 60°C, achieving an 89.80% removal efficiency of phenanthrene in soil. The presence of OH − increased the system pH and decreased the removal efficiency. These results demonstrate that C 12 -MADS significantly enhanced the oxidation of phenanthrene and improves SPS utilization efficiency, contributing to the development of an effective surfactant-assisted persulfate system for soil decontamination.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
·
2017
·
IF 3.798
Jing Li,Qingxiang Zhou,Yongli Liu,Man Lei
In this study, nanoscale zero-valent iron nanoparticles (NZVIs) were coated with silica and polydopamine using a two-step process. The coated nanoparticles were applied as adsorbents for removal of two common polycyclic aromatic hydrocarbons pollutants, phenanthrene (PHE) and anthracene (ANT) from aqueous system. Adsorption kinetics followed a pseudo-second-order model. Isotherms and thermodynamics were investigated and the results indicated that the adsorption process fit best to the Freundlich model and exhibited the characteristics of an exothermal physical adsorption process. Owing to their superparamagnetic characteristics and stability, these adsorbents could be easily collected and recycled for reuse.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）