Food Bioscience
·
2024
·
IF 4.8
Zhengming Xu,Xi Zhu,Jiayi Lian,Yuan Guan,Zhengang Zhao
Exploiting efficient tyrosinase inhibitors and utilizing the inhibition mechanism are of great significance to avoid enzymatic browning in food and skin melanogenesis. Polydatin had excellent tyrosinase inhibitory activity in vitro with IC 50 value of 24.90 μM; however, it exhibited unsatisfactory inhibitory activity intracellularly, which was associated with poor cellular uptake. Fortunately, 6″- O -lauryl polydatin was green-efficiently synthesized by lipase-catalyzed acylation under microwave irradiation. 6″- O -lauryl polydatin exhibited superior mono-/diphenolase inhibitory activity within 100 μM than kojic acid, which had IC 50 of 221 μM. Neither polydatin nor 6″- O -lauryl polydatin bound to the catalytic site, but rather bound noncovalently to the allosteric site 2 of tyrosinase through hydrogen bonds and hydrophobic interactions according to molecular simulations, thereby inducing secondary structure unfolding and reducing tyrosinase activity. 6″- O -lauryl polydatin showed stronger anti-browning effects than polydatin in the natural storage of potato and apple pieces. Polydatin and kojic acid did not induce apoptosis, but 6″- O -lauryl polydatin inhibited human melanin A375 cell proliferation by stimulating ROS overproduction and regulating cell cycle and apoptotic pathways. In addition, lipophilic 6″- O -lauryl polydatin had greater cellular uptake though suffered from intracellular hydrolysis, and exerted better tyrosinase and melanogenesis inhibitory activity than polydatin and kojic acid based on mouse B16F10 melanoma cell model. The results suggested polydatin and 6″- O -lauryl polydatin were promising tyrosinase inhibitors for anti-browning in foods or cosmetics fields.
研究领域:
食品科学
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药学
·
化妆品化学
·
酶学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
Jifu Hao,Tiantian Tong,Kai Jin,Qiannan Zhuang,Te Han,Yanping Bi,Jianzhu Wang,Xiaodan Wang
A folic acid (FA)-functionalized drug vehicle platform based on Pluronic 127 (P127)/D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) mixed micelles was orchestrated for an effective delivery of the model drug resveratrol in order to address the problem of poor water solubility and rapid metabolism of resveratrol and improve its targeted accumulation at tumor site. The FA-decorated mixed micelles were prepared using thin-film hydration method and optimized by central composite design approach. The micelles were also characterized in terms of size and morphology, drug entrapment efficiency and in vitro release profile. In addition, the cytotoxicity and cell uptake of the micelles were evaluated in folate receptor-overexpressing MCF-7 cell line. In vivo pharmacokinetic and biodistribution studies were also performed. The average size of the micelles was ~20 nm with a spherical shape and high encapsulation efficiency (99.67%). The results of fluorescence microscopy confirmed the targeting capability of FA-conjugated micelles in MCF-7 cells. FA-modified micelles exhibited superior pharmacokinetics in comparison with that of solution. Further, the low accumulation of resveratrol-loaded FA micelles formulation in the heart and kidney avoided toxicity of these vital organs. It could be concluded that folate-modified P127/TPGS mixed micelles might serve as a potential delivery platform for resveratrol.
研究领域:
药剂学
·
肿瘤学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD SAFETY
·
2020
·Vol. 40
·期 3
·e12777
·
IF 1.133
Yingchang Li,Qian Zou,Suzhen Song,Tong Sun,Jianrong Li
The effects of combined application of lysozyme and resveratrol with chitosan coatings on the quality of Sciaenops ocellatus during refrigerated storage were studied. Physical and chemical indices were periodically analyzed. The chitosan composite coatings were characterized, and the mechanical properties and gas permeability were also measured. The results showed that chitosan coatings could significantly reduce the total viable count (TVC), maintain tissue hardness, chewiness and sensory quality, slow the rate of fat oxidation, and restrain the increases in pH and total volatile basic nitrogen (TVB-N). Compared with chitosan coatings, the addition of resveratrol and lysozyme had increased the tensile strength and reduced the gas permeability. The lysozyme–resveratrol–chitosan coatings had good preservation properties. The study indicated that the 0.1% lysozyme + 0.2% resveratrol + 1% chitosan group of composite coatings could maintain the quality and extend the shelf life for 8 days compared with the control group during refrigerated storage.
研究领域:
食品科学
·
生物学
✓ 已验证
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Linlin Liu,Min Shi,Yanyan Wu,Jiali Hao,Junxiu Guo,Shanshan Li,Pingli Dai,Jing Gao
The widespread use of pesticides poses a significant threat to honeybee health by impacting their survival, behavior, immune function, and detoxification capacity. While phytochemicals such as resveratrol (RSV) have shown potential in mitigating oxidative stress and enhancing antioxidant defenses, their role in improving honeybee tolerance to pesticide exposure remains underexplored. In this study, we investigated the effects of RSV supplementation on honeybees exposed to three pesticides: dinotefuran (DIN), tebuconazole (TEB), and deltamethrin (DEL). The results showed that RSV supplementation significantly improved survival, feed intake, mobility, and gustatory sensitivity, indicating its protective effects against pesticide toxicity. Furthermore, RSV helped normalize impaired detoxification enzyme activities, including SOD, POD, catalase, and glutathione reductase, and reduced ROS levels and lipid peroxidation. Gene expression analysis revealed that RSV modulates Toll pathway-related genes like defensin and apidaecin , alleviating immune suppression caused by pesticides. Additionally, RSV influenced the insulin/insulin-like growth factor signaling (IIS) pathway by reducing ilp1 and inr1 expression, potentially mitigating metabolic stress. These findings demonstrate that protective effects of RSV may be linked to its ability to counter oxidative stress, restore mitochondrial function, and enhance energy metabolism. Furthermore, RSV is widely available, cost-effective, and easily incorporated into bee feed, making it feasible for large-scale application. This study highlights the protective role of RSV in pesticide detoxification in honeybees, offering new perspectives for honeybee health management and environmental toxicology research. By reducing the adverse effects of pesticides on honeybees, the application of RSV not only contributes to maintaining ecological balance but also supports sustainable agricultural practices. Future research should focus on optimizing its dosage, evaluating long-term effects, and investigating its impact on colony dynamics to facilitate its practical implementation in apiculture.
研究领域:
昆虫学
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环境毒理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Discover Oncology
·
2025
·Vol. 16
·期 1
·1-23
·
IF 2.9
Jiang Yingcun,Song Chao,Yan Jiyuan,Luo Liang,Gao Silong,Jiang Feng,Wei Zhangchao,Chen Jinwen,Liu Zongchao,Ge Jianhua
Ferroptosis has been demonstrated to have a significant role in osteosarcoma (OS), a highly aggressive and invasive malignant bone tumor. Nevertheless, the precise molecular mechanism underlying OS remains unknown. Understanding the makeup of the immune microenvironment in OS is crucial for its therapy, as the disease grows in the highly specialized, complex, and dynamic bone microenvironment. Resveratrol (Res) possesses anti-inflammatory, immunomodulatory, chemopreventive, antioxidant, and anticancer properties, it is unknown if it can modify ferroptosis to prevent OS. This time, using single-cell analysis and other bioinformatic studies, we will clarify the targets and composition of the immunological microenvironment of the ferroptosis process in OS, as well as the role of certain transcription factors in it. Ultimately, network pharmacology and vitro experiment have led to the initial identification of the molecular processes governing ferroptosis in OS, which are regulated by Res. The findings suggested the potential use of ALB, EGFR, GPX4, IL6, STAT3, and PTEN as OS prognostic and diagnostic biomarkers. Chondroblastic, myeloid cells, osteoblastic OS, CD4 + T, NK, CD8 + T, B cells, M1 macrophages, Chondro_Proli, etc. made up the majority of the immunological microenvironment of OS. The entire cellular trajectory demonstrates that immune cells infiltrating during the early stages of OS are mostly CD4 + T, NK, CD8 + T, B_cell, and M1 macrophages. This affects the development of myeloid cells and chondroblastic cells, which ultimately leads to the progression of highly malignant chondro cells to OS. Numerous pathways allow transcription factors including BCLAF1, MAF, SP1, TCF12, KLF11, and KMT2D to contribute to the development of tumors. Finally, by interacting with the aforementioned targets, cells, Res is thought to impede the evolution of OS. In conclusion, ferroptosis and alterations in the immunological milieu are significant factors in the development of OS, and Res may one day be employed as a therapeutic drug to treat OS.
研究领域:
肿瘤学
·
生物信息学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Tianfang Duan,Zeru Xu,Shanbai Xiong,Hongying Du
BACKGROUND Chitosan (CS), an abundant alkaline polysaccharide, is valued for its biocompatibility, non-toxicity, and antibacterial properties. However, its limited solubility and modest antioxidant activity constrain its utility. Grafting polyphenols onto chitosan through the use of grafting reactions can enhance both the solubility and bioactivity of chitosan. Among the techniques employed, the free radical grafting method is favored for its simplicity, environmental sustainability, and its effectiveness in preserving biological activity. RESULTS In this study, chlorogenic acid (CGA) and polydatin (PLD) were conjugated successfully to chitosan by a Vc/H 2 O 2 redox system. Analytical techniques such as ultraviolet-visible (UV-visible) spectroscopy, fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and proton nuclear magnetic resonance ( 1 H NMR) were employed to confirm the formation of covalent bonding between the polyphenol molecules and the chitosan backbone. The novel conjugates displayed superior antioxidant properties in comparison with pristine chitosan, as evidenced by their enhanced 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, and hydroxyl radical scavenging capacities, and Fe 2+ reducing power. Both CGA-CS and PLA-CS exhibited excellent lipid and protein oxidation inhibition capabilities. Furthermore, the conjugates were shown to have significant antibacterial effects against four common pathogenic bacteria: Pseudomonas fluorescens , Pseudomonas aeruginosa , Pseudomonas putida , and Staphylococcus aureus ( P < 0.05). CONCLUSION The newly synthesized water-soluble polyphenol-chitosan conjugates demonstrated remarkable biological activity, particularly CGA-CS. This study offers new insights and a strong theoretical foundation for developing natural food preservation materials with potential applications in the food industry. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Yifan Song,Shumin Hu,Lingyu Zhu,Jiayuan Wang
The successful preparation of starch-based Pickering emulsions typically relies on the use of high-viscosity or high-boiling-point oil solvents. However, these solvents present challenges in removal and limit the applicability of the emulsions. This study aims to investigate the feasibility and effectiveness of employing small-molecule solvents as alternatives for the oil phase. Specifically, quinoa starch granules are used as the emulsifying agent, with a systematic exploration of various small molecules from different solvent categories. This research contributes by identifying the impact of solvent-particle interactions on the feasibility of emulsion formation—an aspect critical but often overlooked in previous discussions on solvent influence on emulsion quality, essential for guiding solvent selection. Furthermore, to address potential challenges associated with maintaining emulsion stability when using low-viscosity oils, a strategy based on exploiting depletion attraction is proposed and demonstrated, leveraging bovine serum albumin as depletants.
研究领域:
食品科学
·
胶体化学
·
界面科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 6
·988
·
IF 5.1
Shengyuan Guo,Chaofan Zhao,Jiankang Zhou,Zhuo Zhang,Wenting Wang,Yuting Zhu,Chuan Dong,Guixing Ren
In order to explore the effect of the content of nutritional components of quinoa on its sensory quality, 22 quinoa varieties were collected from 11 major quinoa-producing areas at home and abroad as experimental materials. The contents of total starch, protein, fat, crude fiber, ash, VB1, VB2, moisture and saponin were determined, and the sensory evaluation and electronic tongue analysis of cooked quinoa were carried out. The sensory quality of quinoa was comprehensively evaluated by correlation analysis, principal component analysis and cluster analysis. The results show that the contents of various nutritional components had significant effects on the sensory quality of quinoa (p< 0.05). Quinoa with high starch, high VB1, moderate fat, moderate moisture, low protein, low crude fiber, low ash and low saponin content had better cooking quality and was more popular. Electronic tongue analysis showed that the sweet response value of cooked quinoa was the highest, followed by the bitter response value. No. 12 and No. 9 cooked quinoa samples had the best comprehensive taste, the highest sensory score and the best cooking quality. This study preliminarily clarified the relationship between the content of different nutritional components in quinoa and its sensory quality, which could provide reference for the selection of raw materials and breeding of quinoa varieties for different processing purposes.
研究领域:
食品科学
·
植物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2020
·Vol. 12
·期 12
·2794
·
IF 3.426
Yunliang Xing,Xianru He,Rui Yang,Kan Zhang,Shengfu Yang
A novel resveratrol-based bio-benzoxazine monomer (RES-al) containing an allyl group has been synthesized using resveratrol, allylamine, and paraformaldehyde via Mannich condensation reaction, and its chemical structures have been characterized by FT-IR spectroscopy and NMR techniques. The polymerization behavior of this benzoxazine resin has been investigated using in situ FT-IR and differential scanning calorimeter (DSC) measurements, and the thermal-mechanical properties of its corresponding polybenzoxazines are evaluated by DMA and TGA. We show that by controlling the curing process of the oxazine ring, the C=C bond in resveratrol, and the allyl group in RES-al, the cross-linking network of the polybenzoxazine can be manipulated, giving rise to tunable performance of thermosets. As all curable functionalities in RES-al are polymerized, the resulted polybenzoxazine exhibits a good thermal stability with aTgtemperature of 313 °C, a Td5value of 352 °C, and char yield of 53% at 800 °C under N2.Keywords:benzoxazine;resveratrol;polybenzoxazine;allyl;high-performance
研究领域:
材料科学
·
有机化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2019
·Vol. 74
·期 5
·1377-1382
·
IF 1.246
Zhang Xue,Wang Yanhe,Zhang Zhuanzhuan,Xia Wenlong,Song Xiaofeng,Ma Liwei
Resveratrol which is a natural polyphenol with good biological activity is mainly used in medicine, but it has poor photostability in vitro and short plasma half-life in vivo. Multi-armed poly( ε -caprolactone) (PCL) has attracted great attention because the increased number of terminal group broadens the polymeric flexibility in biomedical application. In the work, six-armed PCL was coupled with resveratrol through the hydrolysable ester bond to solve its disadvantage and realize protection for more resveratrol. The synthesized product was characterized with 1 H NMR, GPC and DSC. The molecular weight of the designed polymer was less than the experimental, and the polymeric distribution of molecular weight was narrow. In addition, resveratrol changed the physical form of six-armed PCL. The resveratrol coupling with six-armed PCL presented better photostability compared to original resveratrol. The concentration curve of the coupled resveratrol extended to the plain terrain after 80 min of irradiation, while the original resveratrol continued to decline. They were 72.10% and 52.35% at 2 h, respectively. Moreover, six-armed PCL could effectively protect resveratrol in vivo, and buy time for reaching the target site. The in vitro released amount of the original resveratrol was 52.89% within 3 h, while the coupled resveratrol was only 6.28%. Then this study provides a promising strategy for efficient usage of resveratrol.
研究领域:
药学
·
高分子化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2019
·
IF 3.621
Kan Zhang,Mengchao Han,Lu Han,Hatsuo Ishida
A series of thermally stable resveratrol-based tri-functional benzoxazine resins have been synthesized using resveratrol , different amines (aniline, 4-chloroaniline and 3-aminophenylacetylene) and paraformaldehyde. The molecular structures of these benzoxazines have been characterized by 1 H and 13 C NMR spectroscopy and FT-IR spectroscopy. The assignments for the characteristic protons and carbons in oxazine rings are confirmed using two-dimensional (2D) NMR techniques, including 1 H– 1 H NOESY and 1 H– 13 C HMQC. Moreover, the ring-opening polymerization of benzoxazines is studied using in situ FT-IR and differential scanning calorimetry (DSC), and the thermal stability and the flammability of the polybenzoxazines are also investigated by thermogravimetric analysis (TGA) and micro-scale combustion calorimetry (MCC), respectively. In addition to the advantages of lowest melting and polymerization temperatures amongst these resveratrol-based, tri-functional benzoxazines, the corresponding polybenzoxazine derived from benzoxazine containing acetylene shows highest thermal stability with a Tg temperature higher than 350 °C, initial degradation temperature at 10% weight loss at 465 °C, and char yield of 74% at 800 °C in nitrogen atmosphere. Furthermore, this polybenzoxazine also exhibits exceptionally low heat release capacity (30.7 J g −1 K −1 ) and total heat release value (6.0 kJ/g).
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Limin Xiang,Lingyun Xiao,Yihai Wang,Haifeng Li,Zebo Huang,Xiangjiu He
Moderate consumption of red wine reduces the risk of heart disease and extends lifespan, which these healthy benefits are often attributed to its high antioxidant content. The relative contributions of wine polyphenols in healthy benefits were studied in this study. Among all wine polyphenols, caffeic acid was the richest one, while gallic acid showed the highest free radical scavenging activity. There was no significant difference between the prime red wine and the red wine adding 10-fold resveratrol on neuroprotective effects on SH-SY5Y cell line. The contribution percentage of resveratrol to the antioxidant activity of red wine was less than other tested polyphenols. It suggested that resveratrol may be negligible with respect to healthy benefits of red wine.
研究领域:
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）