RSC Advances
·
2020
·Vol. 10
·期 47
·28408-28414
·
IF 3.119
Heming Zheng,Bo Duan,Zheyu Xie,Jie Wang,Mingying Yang
Bombyx mori (B. mori) silk fibroin (SF) microcapsules have acted as a great candidate in delivering drugs. However, it is difficult to fabricate SF nanocapsules using the present layer-by-layer (LBL) technique. In addition, the current SF microcapsules have limits in loading negatively charged drugs. Here, we invent a novel LBL method by introducing silane (APTES) as a structure indicator to produce SF nanocapsules that can load drugs with negative or positive charge. LBL assembly was completed by alternately coating SF and APTES on the template of polystyrene (PS) nanospheres by electrostatic attraction. SF nanocapsules were obtained after removal of the PS templates. Zeta potential analysis proved LBL assembly was indeed driven by the interaction between negative charge of SF and positive charge of APTES. Fluorescence images and electric microscope images indicated that SF nanocapsules had a hollow and stable structure with diameter at nearly 250 nm. The highest encapsulation rate of DOX or Ce6 were up to 80% and 90%, respectively, indicating SF nanocapsules have a high loading capability for both cationic and anionic drugs. In vitro cell experiments proved the biocompatibility of SF nanocapsules and their burst drug release in response to acidic environment. Furthermore, chemotherapy and photodynamic therapy proved SF nanocapsules loaded with DOX or Ce6 had significant inhibition on tumor cells. Our results suggested that this LBL technique is a facile method for polymers with negative charge to fabricate nanocapsules for antitumor drug carrier.
研究领域:
生物材料学
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药物递送系统
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肿瘤治疗
✓ 已验证
DOI
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
·
2019
·
IF 2.54
Bo Pang,Xinyu Bian,Junpeng Xing,Shu Liu,Zhiqiang Liu,Fengrui Song
The abnormal aggregation of human islet amyloid polypeptide (hIAPP) is a crucial pathogenic factor associated with type 2 diabetes (T2D). The development of effective inhibitors to prevent hIAPP aggregation is a common therapeutic strategy against T2D. Lithospermic acid (LA) is a natural compound with diversified biological activities. In this study, electrospray ionization coupled with ion mobility–mass spectrometry, thioflavin T fluorescence assay , Congo red binding assay, Nile red fluorescence assay, circular dichroism spectroscopy, transmission electron microscopy , cell toxicity, lactate dehydrogenase assay (LDH) assay and molecular docking were combined to explore the influence of LA on hIAPP aggregation. Results showed that LA had favorable binding affinity to hIAPP and formed hIAPP–LA complexes, which could alter the relative abundance of the compact and extended conformers and promoted the transition of extended structures to compact conformers. LA also displayed strong inhibitory actions on fibrillation and potential protective effects against hIAPP-induced cell toxicity. Therefore, the obtained results were useful to understand the possible inhibitory mechanism of LA on hIAPP aggregation and provided valuable reference for the screening of potent amyloid inhibitors.
研究领域:
生物化学
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药物化学
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糖尿病研究
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2021
·Vol. 14
·期 1
·108
·
IF 4.329
Bo-Xiang Wang,Jia Li,De-Hong Cheng,Yan-Hua Lu,Li Liu
Antheraea pernyisilk fibroin (ASF)-based nanofibers have wide potential for biomaterial applications due to superior biocompatibility. It is not clear whether the ASF-based nanofibers scaffold can be used as an in vitro cancer cell culture platform. In the current study, we fabricated novel ASF-based thermoresponsive hydrogel nanofibers by aqueous electrospinning for colon cancer (LoVo) cells culture. ASF was reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE), which provided the possibility of copolymerization with allyl monomer. The investigation of ASF-AGE structure by1H NMR revealed that reactive allyl groups were successfully linked with ASF. ASF-based thermoresponsive hydrogel nanofibers (p (ASF-AGE-NIPAAm)) were successfully manufactured by aqueous electrospinning with the polymerization of ASF and N-isopropylacrylamide (NIPAAm). The p (ASF-AGE-NIPAAm) spinning solution showed good spinnability with the increase of polymerization time, and uniform nanofibers were formed at the polymerization time of 360 min. The obtained hydrogel nanofibers exhibited good thermoresponsive that the LCST was similar with PNIPAAm at about 32 °C, and good degradability in protease XIV PBS solution. In addition, the cytocompatibility of colon cancer (LoVo) cells cultured in hydrogel nanofibers was assessed. It was demonstrated that LoVo cells grown on hydrogel nanofibers showed improved cell adhesion, proliferation, and viability than those on hydrogel. The results suggest that the p (ASF-AGE-NIPAAm) hydrogel nanofibers have potential application in LoVo cells culture in vitro. This study demonstrates the feasibility of fabricating ASF-based nanofibers to culture LoVo cancer cells that can potentially be used as an in vitro cancer cell culture platform.Keywords:Antheraea pernyisilk fibroin;nanofibers;thermoresponsive;cancer cell;in vitro culture
研究领域:
生物材料学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
FIBERS AND POLYMERS
·
2022
·Vol. 23
·期 8
·2225-2233
·
IF 2.347
Wu Leilei,Bao Xueming,Ren Yiwen,Wang Ping,Zhou Man,Yu Yuanyuan,Wang Qiang
Generally, Bombyx mori silk dyeing is performed using acid dyes. However, the color fastness is always unsatisfactory due to the weak binding forces under neutral conditions. In the present work, graft polymerization of α-lipoic acid (ALA) onto silk fibers was carried out using a two-step method. Briefly, ALA was grafted onto the silk fibers, followed by a reduction-oxidation process to form poly(α-lipoic acid) (PALA) on the fiber surfaces, aiming at improving the electronegativity of fiber surface and realizing cationic dyeing. The results implied that the combined treatments promoted the intermolecular crosslinking between fibroin chains, endowing the silk fiber with low zeta potential simultaneously. A thin sedimentary layer on the fiber surfaces was observed for the PALA-deposited silk fibers, companying with a significant increase in the content of sulfur and O/N ratio, which verified the successful graft polymerization. The modified silk fabric exhibited improved dyeability, and the dyeing depth and color fastness was noticeably higher compared to that of the untreated after cationic dyeing. Meanwhile, the newly-built intermolecular disulfide crosslinks imparted a detectable anti-wrinkle effect to the silk fabric. This study provides a facile strategy for modification of silk fabric with improved dyeing behaviors and physical properties.
研究领域:
材料科学
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纺织工程
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER BULLETIN
·
2025
·
IF 4
Jin Yuwei,Pan Mengyao,Jiang Kexin,Mao Ying,Lu Wangyang,Jiang Wenbin,Chen Wenxing
Artificial diet-rearing silkworms are strongly developed due to climate constraints and market demand. However, research on the industrialized artificial diet-rearing silkworms at all industrialized silkworm rearing) remains relatively limited. This study comprehensively investigated the dissolution properties of sericin derived from industrialized silkworm rearing cocoons. The physicochemical features were systematically characterized through amino acid analyses, molecular weight distribution tests, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). Under identical degumming conditions, the sericin from industrialized silkworm rearing cocoons exhibited superior dissolution properties, particularly faster dissolution speed and less remaining sericin content than that from mulberry leaf-rearing silkworm cocoons. In comparison, no significant differences were observed in amino acid composition, secondary structure, and thermal stability between sericin samples from these two cocoons. Importantly, for industrialized silkworm rearing cocoons, degumming treatment and sericin extraction will consume less energy, aligning with the principles of low-carbon production and environmental protection. Additionally, this work highlights the broad development potential and application prospects of industrialized silkworm rearing and the resulting sericin. Graphical abstract
研究领域:
材料科学
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生物技术
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高分子科学
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纺织工程
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Zhenhu Guo,Wensheng Xie,Qin Gao,Dan Wang,Fei Gao,Shengrong Li,Lingyun Zhao
Possessing excellent biocompatibility, biodegradability and good reactive activity, silk fiber has been attracting great attention in biomedicine including surgical suture , drug delivery and tissue engineering . So far, several protocols have been developed to further improve the mechanical properties of the silk fiber. In current study, a novel in suit biomineralization strategy was developed to produce nano-hydroxyapatite (HA) strengthened silk fiber based on the natural alkaline condition in the body of silkworm by feeding the silkworm with ion precursors of Ca 2+ and PO 4 3− ions. Our observation proved that nanocomposite silk fiber contained more α-helix and random coil structures and fewer β-sheets. Tensile test showed that such obtained silk fiber has superior mechanical properties compared to normal silk fiber. To the best of our knowledge, no attempts have been made to fabricate the nanocomposite SF by in situ biomineralization and such protocol established in the current study may shed light on the investigation of nanoparticles reinforced silk fiber organisms of Bombyx mori .
研究领域:
生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
CELLULAR POLYMERS
·
2014
·
IF 0.6
Wenming Zhang,Ling Li,Qing Li,Xiaowei Li,Yucang Zhang
In this study, Faba bean stalk was liquefied for the production of biopolyols and polyurethane (PU) foams. The influences of various reaction conditions on biopolyols and PU foams were examined. Furthermore, the property relationships between biopolyols and PU foams were investigated. Biopolyols produced under the liquefaction conditions of 160°C, 90 min, 3% sulfuric acid loading, and 20% Faba bean stalk loading showed promising material properties (i.e. hydroxyl number, 496.1 mg KOH/g, acid number, 17.9 mg KOH/g, and viscosity, 1.1 Pa.s). PU foams produced from promising biopolyols showed compressive strength of 205 kPa and density of 0.035 g/cm3. The results suggested that in addition to density, the compressive strength of PU foams might also be affected by factors such as biomass residues or the chemical structures of biopolyols. The biopolyols and PU foams produced showed material properties comparable to those of conventional PU foams.
研究领域:
材料科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Tan Xue,Qi Wang,Panpan Zhang,Yan Miao,Jianing Sun,Fengyu Dong,Xinjing Gui,Jing Yao,Ruixin Liu
This study investigates the consistency between the formula granule decoction of stir-fried Bombyx Batryticatus(JC-DGD) and its traditional decoction (JC-TD) counterpart, using roasted Bombyx Batryticatus as a representative example.First, used high-performance liquid chromatography (HPLC) to establish the fingerprints of JC-TD and JC-DGD from four manufacturers (A, B, C, and D) and assess their similarity. There was no significant difference between the two ( P > 0.05).Quantified five key index components, including uracil.Compared the protein and crude polysaccharide contents using the Coomassie Brilliant Blue and phenol-sulfuric acid methods. The mean total content of index components, proteins, and crude polysaccharides in 10 batches of JC-TD was set as 1. The contents of index components in different categories - JC-DGD-provincial standard(JC-DGD-PS), JC-DGD-enterprise standard(JC-DGD-ES), TD, DGD, and DGCM from manufacturers A, B, C, and D—were 1.23, 0.38, 1.00, 0.72, 1.28, 0.58, 0.45, and 0.27, respectively. The protein contents were 1.51, 0.46, 1.00, 0.88, 1.43, 0.93, 0.35, and 0.66, respectively. The crude polysaccharide contents was 9.01, 0.74, 1.00, 4.05, 10.57, 1.96, 0.38, and 1.77, respectively. We identified 32 differential volatile organic compounds, including fenugreek lactone, using GC-IMS. Then evaluated the anticonvulsant effects of JC-TD and JC-DGD using an acute convulsion mouse model induced by pentylenetetrazole. The results showed that JC-DGD-A > JC-TD ≈ JC-DGD-B ( P > 0.05)) were superior to those of JC-DGD-C and JC-DGD-D ( P < 0.01). Finally, calculate the recommended equivalent ratio of Chinese medicine formula granules (DGCM). The manufacturers A, B, C, and D were adjusted from 1:3, 1:10, 1:10, and 1:11–1:4.54 ± 0.51, 1:5.24 ± 2.22 (JC-B3 without adjustment: 1:3.6), 1:5.17 ± 0.49, and 1:3.44 ± 0.59, respectively. The overall, and the consistency between the two was analyzed through correlation analysis, cluster analysis, and multi-index dimensionality reduction discriminant analysis.Comprehensive results indicated that the quality of JC-DGD-A was significantly higher than that of JC-TD, while JC-DGD-B exhibited similar quality to JC-TD. In contrast, the quality of JC-DGD-C and JC-DGD-D was significantly lower than that of JC-TD. To enhance product quality and ensure the scientific rationality of clinical applications, manufacturers must adhere to national and provincial standards for formula granule drugs.
研究领域:
中药学
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2020
·Vol. 32
·期 34
·2002695
·
IF 27.398
Zongpu Xu,Mingrui Wu,Weiwei Gao,Hao Bai
Flexible and transparent substrates play a fundamental role as a mechanical support in advanced electronic devices. However, commonly used polymer films, such as polydimethylsiloxane, show low tear resistance because of their crack sensitivity. Herein, inspired by the excellent mechanical robustness of the skin and its fibrous structure, an epoxy-resin-based composite with a flat silk cocoon as a reinforcing fiber network is fabricated. With only 1 wt% of silk fiber, the tensile strength and modulus of the as-prepared composite film are considerably increased by 300% and 612% compared to those of pure resin, while still maintaining flexibility and transparency. More importantly, the composite shows remarkable tear resistance: without fracture after ≈30 000 tensile cycles. The potential application of such transparent composite films as mechanically robust substrates for flexible electronics is also demonstrated. In addition, this study represents a bioinspired strategy to construct high-performance functional composite materials.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 7
·3558-3569
·
IF 3.049
Hao Xu,Wenhui Yi,Dongfan Li,Ping Zhang,Sweejiang Yoo,Lei Bai,Jin Hou,Xun Hou
Silkworm fibers have attracted widespread attention for their superb glossy texture and promising mechanical performance. The mechanical properties can be reinforced with carbon nanofillers, particularly carbon nanotubes (CNTs), depending on the CNT content in the silk fibers. In order to increase the CNT content, lignosulfonate (LGS) was used as a surfactant to ameliorate the CNT solubility, dispersibility, and biocompatibility. The resulting CNT/LGS nano-composite was further processed through an additional purification method to remove excess surfactant and enhance the CNT/LGS ratio. Then the purified biocompatible single and multiple-walled CNTs were fed to silkworms, leading to a large CNT content in the resulting silk fibers. Reinforced silk fibers were produced with a mechanical strength as high as 1.07 GPa and a strain of 16.8%. The toughness modulus is 1.69 times than that of the unpurified group. The CNT-embedded silk fibers were characterized via Raman spectrometry and thermogravimetric analysis (TGA), demonstrating that the CNT content in the silk fibers increased 1.5-fold in comparison to the unpurified group. The increased CNT content not only contributed to the self-assembly into buffering knots of silk fibers, but it also enhanced the conductivity of graphitized silk. Our coating and purification strategies provide a potential facile way to obtain natural silk fibers with high mechanical performance.
研究领域:
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysts
·
2023
·Vol. 13
·期 6
·987
·
IF 3.9
Haoyu Wang,Chunyong Zhang,Zhe Li,Yinpin Wen,Li Shu
In this work, a NixCox/Silk-PVDF bimetallic catalyst electrode was prepared for the hydrogen evolution reaction (HER) in hydropower. This cheap, durable, and efficient electrode has good practical application prospects. Green natural silk, which will pollute the environment. The electrodes (obtained by varying the Ni:Co ratio and hydrothermal times) were prepared hydrothermally. Ni and Co elements were revealed by X-ray diffraction, X-ray photoelectron spectroscopy, and transmission electron microscopy. Ni2Co2/silk-PVDF was identified as an effective catalyst in 1 M KOH alkaline electrolyte with an overpotential of 89.4 mV at 20 mA cm−2and a Tafel slope of 47.46 mv dec−1. It showed low resistance and a high specific surface area in EIS and CV tests, respectively, proving its superior HER performance. Finally, the stability and durability of the electrode coated with PVDF were demonstrated via testing at a voltage of −0.1 V over 24 h. This work provides an environmentally friendly and simple method to load metal on a self-supporting electrode to be used in the hydrogen evolution reaction.Keywords:silk;PVDF;bimetallic electrocatalyst;electrochemical;HER
研究领域:
材料科学
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yu Zhang,Tonghe Xing,Zhiyu Huang,Annan He,Yuxin Luo,Ying Hong,Mengqi Wang,Zhicheng Shi,Aixin Tong,Sijie Qiao,Guizhen Ke,Tao Zhao,Fengxiang Chen,Weilin Xu
As a luxurious textile raw material, silk fiber (SF) and its products are widely favored by consumers because of their elegant appearance and excellent comfort. However, their flammability causes potential fire risks and hazards in daily fires and has gained significant attention in recent years. In this work, an ultrathin and uniform TiO 2 , Al 2 O 3 , and ZnO nanofilms with thicknesses of 1 ∼ 200 nm (less than one in a hundred of SF diameter) were deposited on the surface of a silk fabric (SFR) by atomic layer deposition (ALD). By tuning the types of metal oxides and nanofilm thickness, the modified SFR quickly self-extinguished, and its pristine weave was completely preserved after exposure to a flame in a torch burn test, representing superior performance compared to numerous conventional coating technologies reported with higher loadings. An expanded charcoal residue formed on the surface of the modified SFR, acting as a physical barrier that enhanced flame retardancy. Furthermore, the modified SFR showed excellent laundering durability, which helps improve the service life of the fabric. Most importantly, the nanofilms had a negligible impact on the intrinsic comfort and other comprehensive properties of the silk.
研究领域:
材料科学
·
纺织工程
·
表面化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）