ACS Applied Polymer Materials
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2024
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IF 4.4
Ranran Kong,Sicheng Cao,Jincheng Zhang,Rongliang Ma,Li-Juan Fan
Finding a universal fingermark developing strategy effective for all types of fingermarks may improve the efficiency of forensic investigation. Here, we synthesized a fluorescent conjugated polymer (PTPEPE-IDA) with poly[p-(tetraphenylethene)-alt-(phenylene ethylene)] as the backbone and carrying pendant disodium iminodiacetate and monocarboxylate groups to interact with sweat, which is commonly present in fingermarks. PTPEPE-IDA was dissolved in ethanol to form the developing reagent, which was demonstrated to be effective in visualizing and enhancing sweat-containing sebaceous and blood fingermarks on various substrates with the same developing protocol. With the increase of the sweat content in fingermarks, the fluorescence intensity and clarity of patterns of the developed fingermarks increased. This strategy was effective for sweat-containing fingermarks aged for up to 100 days. The developed fingerprint has high stability, being recognizable after storage for 100 days or immersion in water (or ethanol). Mechanism investigation suggests that PTPEPE-IDA can bind some components in sweat and blood through synergistic effects of multiple interactions, and the aggregation-induced emission enhancement (AIEE) upon sweat was demonstrated by the macroscopic view and emission/absorption spectra, which is beneficial to detect the fingermark quickly and then enhance the legibility of the pattern. This universal strategy is expected to make fingermark development more efficient.
研究领域:
法医学
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材料科学
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化学
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 11
·1719
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IF 4.7
Yadan Chen,Yanbin Wang,Liang He,Liling Wang,Jianchen Zhao,Zhenya Yang,Qin Li,Rui Shi
This study aimed to investigate the efficacy of supercritical CO2(SC-CO2) extraction in enhancing the extraction rate, purity, and antioxidant activity ofIndocalamus latifolius(Keng) McClure (Poaceae) leaf terpenoids (ILLTs). Crude extracts obtained from leaves were subjected to qualitative and quantitative analyses, revealing neophytadiene, phytol, β-sitosterol, β-amyrone, squalene, and friedelin as the primary terpenoid constituents, identified through gas chromatography–mass spectrometry (GC-MS). Compared with steam distillation extraction (SD), simultaneous distillation extraction (SDE), ultra-high pressure-assisted n-hexane extraction (UHPE-Hex), ultra-high pressure-assisted ethanol extraction (UHPE-EtOH), ultrasound-assisted n-hexane extraction (UE-Hex), and ultrasound-assisted ethanol extraction (UE-EtOH), SC-CO2exhibited a superior ILLT extraction rate, purity, and antioxidant activity. Scanning electron microscopy (SEM) observations of the residues further revealed more severe damage to both the residues and their cell walls after SC-CO2extraction. Under optimal parameters (4.5 h, 26 MPa, 39 °C, and 20% ethyl alcohol), the ILLT extraction rate with SC-CO2reached 1.44 ± 0.12 mg/g, which was significantly higher than the rates obtained by the other six methods. The subsequent separation and purification using WelFlash C18-l, BUCHI-C18, and Sephadex LH-20 led to an increase in the purity of the six terpenoid components from 12.91% to 93.34%. Furthermore, the ILLTs demonstrated cytotoxicity against HepG2 cells with an IC50value of 148.93 ± 9.93 μg/mL. Additionally, with increasing concentrations, the ILLTs exhibited an enhanced cellular antioxidant status, as evidenced by reductions in both reactive oxygen species (ROS) and malondialdehyde (MDA) levels.
研究领域:
植物化学
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药理学
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分析化学
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DOI
PubMed
高影响因子
METABOLIC ENGINEERING
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2025
·
IF 6.8
Yapeng Zhang,Chenwen Liu,Wenqiang Li,Zhidong Ma,Bo Lv,Lei Qin,Chun Li
β-caryophyllene, a plant-derived sesquiterpene, serves as a food flavoring, anti-inflammatory agent, antioxidant, and high-energy fuel source. Extraction of β-caryophyllene from plants is a costly and inefficient process. Therefore, microbial cell factories have been employed for the production of β-caryophyllene. However, the limited yield is insufficient for its industrial application. In this study, we balanced the utilization of cellular resources for growth and production by systematically regulating the sterol synthesis pathway to maximize the synthesis of β-caryophyllene. In the competitive pathways concerning sterol and fatty acid synthesis, genes expression was suppressed by substituting the original promoters with a glucose-sensing promoter P HXT1 and a sterol synthesis promoter P ERG7 , respectively. This approach effectively increased the production of β-caryophyllene by 6.8 times, reaching 854.7 mg/L. Engineering glucose-sensing pathway altered the strength of P HXT1 , resulting in an increase in β-caryophyllene production to 1.25 g/L. The cell growth and β-caryophyllene production were further boosted through diploid fusion, resulting in 21.4 g/L β-caryophyllene in fed-batch fermentation. This represents the highest reported production of β-caryophyllene to date. This study provides a valuable reference for the production of sesquiterpenes in microbial cell factories.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Qile Chen,Tong Ye,Shujuan Yang,Linwang Fan,Chaonan Shang,Yuhong Feng,Jiacheng Li,Yujuan Wang,Gaobo Yu,Jun Dai
The effective management of Type 2 Diabetes Mellitus (T2DM) depends on the development of streamlined, sustainable, and intelligent delivery systems. In this context, targeted stimulus-responsive Pickering emulsions (PEs) derived from biomass have emerged as a promising candidate. However, the interfacial films of PEs exhibit insufficient strength to endure the harsh gastric environment and lack the capability for targeted and responsive delivery. To address these limitations, an amphiphilic phenylboronic acid-functionalized sodium alginate (SA-PBA) was integrated into a ternary complex platform composed of soybean protein-isolated polyphenol (SPI-PSPAs), where it self-assembled into soft colloidal particles through multiple non-covalent interactions. A multiscale coupling methodology was proposed to investigate and elucidate the structure-function relationship between interfacial characteristics (e.g., thickness, viscoelasticity, and microstructures) and the stability of PEs, thereby providing a foundation for effectively controlling their targeted delivery properties. Notably, owing to the enhanced strength of the interfacial composite particle films, the PEs demonstrated the ability to withstand the harsh gastric environment while maintaining their structural integrity. Furthermore, the self-assembly process of the soft SA-PBA@SPI-PSPAs colloids could be modulated by external glucose stimuli, enabling the targeted and intelligent release of PSPAs in the intestinal environment. Consequently, the well-designed PE system achieved intestinal-targeted responsive release effects, highlighting the potential of the natural α-glucosidase (αG) inhibitor PSPAs as a viable clinical treatment for T2DM.
研究领域:
食品科学
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生物医药
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材料科学
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产品关联
DOI
PubMed
高影响因子
Cell Host & Microbe
·
2024
·
IF 20.6
Tianze Yu,Xiaoqiang Xu,Yang Liu,Xiaokai Wang,Shi Wu,Zhuoqiong Qiu,Xiaochun Liu,Xiaoyu Pan,Chaoying Gu,Shangshang Wang,Lixin Dong,Wei Li,Xu Yao
Cutibacterium acnes is the most abundant bacterium of the human skin microbiome since adolescence, participating in both skin homeostasis and diseases. Here, we demonstrate individual and niche heterogeneity of C. acnes from 1,234 isolate genomes. Skin disease (atopic dermatitis and acne) and body site shape genomic differences of C. acnes , stemming from horizontal gene transfer and selection pressure. C. acnes harbors characteristic metabolic functions, fewer antibiotic resistance genes and virulence factors, and a more stable genome compared with Staphylococcus epidermidis . Integrated genome, transcriptome, and metabolome analysis at the strain level unveils the functional characteristics of C. acnes . Consistent with the transcriptome signature, C. acnes in a sebum-rich environment induces toxic and pro-inflammatory effects on keratinocytes. L-carnosine, an anti-oxidative stress metabolite, is up-regulated in the C. acnes metabolome from atopic dermatitis and attenuates skin inflammation. Collectively, our study reveals the joint impact of genes and the microenvironment on C. acnes function.
研究领域:
微生物学
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皮肤病学
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基因组学
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DOI
PubMed
高影响因子
Materials Horizons
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2024
·
IF 12.2
Hao Liu,Zhiming Liu,Hao Zhang,Ke Huang,Xiaohui Liu,Hui Jiang,Xuemei Wang
The non-homeostasis of sebum secretion by the sebaceous glands in a complicated microenvironment dramatically impacts the skin health of many people in the world. However, the complexity and hydrophobicity of sebum mean a lack of diagnostic tools, which makes it challenging to determine the reason behind cortical imbalances. Herein, a biomimetic mineralized aggregates (PTL@Au and PTB@Au) strategy has been proposed, which could obtain molecular information about sebum by surface-enhanced Raman spectroscopy (SERS). The breaking of disulfide bonds leads to changes in hydrogen bonding, which transform the natural protein into amyloid-like phase transition protein with β-sheets. It provides sites for the nucleation and crystallization of gold nanocrystals to build mineralized aggregates. The mineralized aggregates show robust adhesion stability at the interfaces of different materials through hydrogen bonding and electrostatic interactions. The stabilization, hydrophobicity (contact angle: 134°), and optical transmission (75%) of the structure could result in superior SERS performance for sebum analysis. It should be noted that this enables the sebum detection of clinical samples while ensuring safety. Such generalized bionic mineralization construction and diagnosis methods also serve as an advanced paradigm for a range of biomedical applications.
研究领域:
皮肤病学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
POLYMER
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2024
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IF 4.1
Rui Hong,Ri-Tong Lan,Yue Ren,Lu Xu,Yun-Xiang Xu,Nouman Ali Shah,Rizwan M. Gul,Shishu Huang,Lingli Li,Jia-Zhuang Xu,Zhong-Ming Li,Ka Li
Oxidation resistance is strongly necessary for the use of crosslinked ultrahigh molecular weight polyethylene (UHMWPE) in artificial joints. The hybrid antioxidant strategy emerged as a promising alternative to the single rival that relies on vitamin E (VE). The goal of this study was to investigate the universality of the antioxidant synergy between VE and sugar alcohol (SA). Three types of SA, including D-sorbitol, maltitol and erythritol, were employed to cooperate with VE. Compared to the use of VE alone, these hybrid antioxidants generally enabled the corresponding UHMWPE composites with prolonged oxidation induction time (OIT) and increased crosslink density. The correlation between OIT and crosslink density of VE/SA-stabilized UHMWPE composites showed low attenuation. The applicability of the hybrid antioxidants to protect UHMWPE against oxidation was further demonstrated using the in vitro clinically relevant accelerated aging. Among the used SA, D-sorbitol exhibited the strongest ability to enhance the synergetic efficiency. The possible mechanism was revealed from the following aspects: the number of secondary hydroxyls per molecular mass and the molecular polarity determined via polarity parameter calculation. These findings provide significant insights for fabricating UHMWPE bearing materials with superior comprehensive performance and extended service longevity.
研究领域:
材料科学
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生物医学工程
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高分子化学
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hongli Li,Na Zhang,Jie Qiang,Yongsheng Cao,Guoyi Qu,Xiuzhu Yu
Olives are prone to deterioration during storage, and suitable storage conditions must, therefore, be established to maintain the quality of the fruit and oil. This study investigated the effect of storing olives at room temperature (23 ± 2 °C), refrigerated (4 °C), and frozen (−20 °C) conditions for 7 d on the quality of virgin olive oil (VOO). No significant differences ( p < 0.05) were observed in the fatty acid, squalene, and phytosterol content of VOO across the three storage conditions. Room temperature storage produced the highest oil yield but resulted in ‘fermentation’ and ‘moldy’ off-flavors. Refrigerated VOO retained a more intense ‘grass’ aroma and higher levels of pigments, α-tocopherol, and total phenols. In contrast, freezing led to significant losses in these components and produced the lowest oil yield. For optimal oil quality, olives should be stored at 4 °C if storage exceeds 24 h.
研究领域:
食品科学
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植物学
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产品关联
DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2026
·
IF 4.4
Xiaochun Zheng,Yajun Li,Zhenhua Liu,Peng Sun,Yaqi Dong,Xue Wang,Yanan Wang,Xiaobing Yang
Patchoulol is a widely used sesquiterpenoid in perfumes, cosmetics, foods and pharmaceuticals. The plant-dependent production is suffering from limited growing area, long seasonal cycle, etc. Microbial production represents a sustainable alternative as it is featured with mild operating conditions and eco-friendliness. Herein, we engineered the oleaginous Rhodotorula toruloides toward patchoulol production. First, the patchoulol biosynthesis baseline was constructed by employing a chimeric enzyme of the Pogostemon cablin originated patchoulol synthase and the native FPPS. Second, the supply of essential intermediates was streamlined by redeploying the mevalonate (MVA) pathway while the recycling of NADPH was enhanced through over-expressing related enzymes. Third, the patchoulol production was further enhanced to 724.8 mg/L, 6.0 mg/L/h and 36.2 mg/g glucose by down-regulating the squalene biosynthesis and tuning the cultivation condition in shake flask. Finally, the production of patchoulol was increased to 1.31 g/L and 13.8 mg/g glucose in the minimal medium in a 3-L bioreactor. Our study demonstrated the potential of R. toruloides in producing patchoulol, and should shed light on the microbial synthesis of other sesquiterpenes.
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Li Li,Yasen Wang,Buqing Wang,Liqun Shen,Yahui Gao,Wei Lin,Zijie Li
Numerous organisms have evolved the ability to utilize light through photoreceptor proteins that mediate diverse biological processes. Currently, several optogenetic sensor systems are widely used in yeast. However, when these systems are applied for gene repression to regulate endogenous yeast gene expression, they typically require the insertion of corresponding target sites near the native promoter of the gene of interest to achieve precise modulation. To address these constraints, a novel blue light-inducible optogenetic tool designated iLight9 was developed, a single-component optogenetic biosensor integrated with the CRISPR-dCas9 platform. The stability of the iLight9 system was further enhanced by employing a strategy involving the addition of a protein degradation tag. The resulting system was designated as iLight9O, which facilitated programmable regulation of distinct genes through the introduction of specific sgRNAs. Subsequently, systematic metabolic engineering strategies were employed to construct an efficient patchoulol-producing cell factory in Saccharomyces cerevisiae . Moreover, a two-step isoprenol utilization (IU) pathway was introduced into the recombinant strain to enhance its capacity for patchoulol biosynthesis. Crucially, the iLight9O system was adopted to dynamically downregulate squalene synthase, a key enzyme in the competing squalene biosynthetic pathway. This optogenetic flux control strategy increased patchoulol titers by 66 % in the IU-optimized strain and 24 % in the MVAIU2 strain, demonstrating significant improvements over static engineering approaches.
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高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Chi Zhang,Zhinan Fan,Hao Zhan,Hong Zhou,Rongliang Ma,Li-Juan Fan
Both latent sebaceous and blood fingerprints may provide valuable information for forensic investigation. To detect both types of fingerprints with no need to predistinguish them, a new adaptive developing strategy was proposed. A cationic conjugated polymer with poly[p-(phenylene ethylene)-alt-(thienylene ethynylene)] backbone (PPETE-NMe3+) was synthesized, which was dissolved in N,N-dimethylformamide (DMF) to form the developing solution. Fingerprints were developed by a simple dropping and incubating process without any pre-/post-treatments. Fluorescent photographs of the developed fingerprints on various substrates demonstrated that this developing strategy was effective for both types of fingerprints on nonporous substrates. Gray value analysis further confirmed the enhancement of the legibility of the fingerprint images. The preliminary mechanism exploration suggested that certain weak interactions, such as hydrophobic interaction and electrostatic interaction, may synergistically contribute to the interaction between the polymer and fingerprint components. The molecular design of the polymer combined with an appropriate solvent endowed the developing system the adaptiveness toward different types of fingerprints. This adaptive developing strategy made the fingerprint-developing process more efficient and may be further extended to more practical application scenes.
研究领域:
法医学
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聚合物化学
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材料科学
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DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 3.475
Pingping Zhou,Yi Du,Nannan Xu,Chunlei Yue,Lidan Ye
With special fragrance and various biological properties, linalool has wide applications in food, pharmaceuticals and cosmetics industries. Microbial biosynthesis has become a promising approach to linalool production, however its efficiency is limited by the limited precursor supply and poor efficiency of linalool synthase. With the goal of enhancing heterologous linalool production in Saccharomyces cerevisiae , protein engineering and metabolic engineering were simultaneously employed to solve these bottlenecks. Linalool-producing yeast was first constructed by introduction of t67OMcLIS selected out from a collection of linalool synthases. The efficiency of linalool biosynthesis was then improved by strengthening the supply of geranyl pyrophosphate (GPP) as precursor via overexpression of the complete mevalonate (MVA) pathway and a GPP synthase variant. To accelerate the conversion of the accumulated GPP to linalool, t67OMcLIS was engineered by means of directed evolution after development of a competition-based color-indicated high-throughput screening method. Expression of the t67OMcLIS variant in the engineered S. cerevisiae with enhanced precursor supply yielded 53.14 mg/L of linalool in biphasic shake-flask culture. This study delivered an efficient linalool-producing yeast cell factory and meanwhile provided efficient strategies for biosynthesis of other valuable monoterpenes.
研究领域:
生物工程
·
代谢工程
·
合成生物学
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DOI
高影响因子
危险品化学品经营许可证（带存储）