Journal of the Taiwan Institute of Chemical Engineers
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2024
·
IF 5.5
Xiao Duan,Qiang Wang,Wenfeng Che,Ting Li,Ke Zhang,Lingna Han,Lihua Song,Wei Guo
Background Doxorubicin (Dox) is a potent anticancer drug with severe side effects to normal tissues. To fabricate a nanomedicine with Dox is a better method to address the side effects of Dox in normal tissues. However, nanomedicines with unapproved materials could not be easily applied in clinic, hence, a nanomedicine fabricated by endogenous materials is a potential method to increase the possibility of clinical application. Methods In this study, a redox-responsive nanomedicine with clinical-applied anticancer of Dox and endogenous molecule of l -glutathione oxidized (GSSG) was prepared for cancer therapy. : The carboxyl and amino groups in l -glutathione oxidized can be reacted itself to form the poly- l -glutathione oxidized peptide-like polymer. Meanwhile, the Dox were conjugated in poly- l -glutathione oxidized polymer by amnio group in Dox and the structure of Dox-conjugated poly- l -glutathione oxidized polymer (Dox-Poly GSSG) was confirmed by FT-IR, NMR and MALDIT-TOF. Afterward, the drug loading of Dox (22 %) in poly-GSSG was measured by UV–vis and the accumulative Dox release rate of Dox-Poly GSSG is 46 % in 5 mg/mL reduced glutathione (GSH) environment at 72 h The size stability of Dox-Poly GSSG in PBS and PBS with GSH was evaluated by dynamic laser scatter (DLS) and transmit emission microscopy (TEM). Furthermore, cell viabilities of Dox-Poly GSSG were similar with free Dox evaluated by CCK-8 assay and flow cytometry experiment, respectively. The distribution of Dox-Poly GSSG in nuclei and tumor tissues was evaluated by Confocal laser scanning microscope and in vivo imaging system, respectively. Significant Findings The drug release results showed that the Dox could be controllable released in high concentration of GSH in tumor tissues. The tumor inhibition, H&E and TUNEL experiments showed that the Dox-Poly GSSG group can significantly inhibit the tumor growth compared to saline group and avoid major side effects to normal tissues compared to free Dox. This redox-responsive nanomedicine with clinical-applied anticancer of Dox and endogenous molecule of GSSG is a potential candidate for clinical application.
研究领域:
药学
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纳米技术
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癌症研究
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药物递送系统
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生物医学工程
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DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Ziyu Zhang,Haolin Li,Jing Xu
The practical application of aqueous zinc-ion batteries (AZIBs) requires addressing the key challenge of temperature range limitations, facing thermodynamics dominated corrosion at high temperatures and kinetics dominated mass transfer obstruction at low temperatures. Although these temperature-related failure mechanisms have been extensively studied, the corrosion of dissolved oxygen (DO) remains frequently overlooked, necessitating further research of wide-temperature failure mechanisms in AZIBs. Herein, we demonstrate that DO corrosion occurs over a wide-temperature range and significantly exacerbates Zn corrosion and byproducts formation, surpassing the well-recognized proton corrosion. We introduce antioxidant glutathione (GSH) as an electrolyte additive to address the corrosion caused by DO and proton. Coexisting glutathione/oxidized glutathione redox couple can spontaneously capture DO and eliminate the generated H2. Moreover, GSH disrupts the intrinsic H-bond networks, depresses the electrolyte freezing point, and enhances the low-temperature mass-transfer kinetics. A self-healing hybrid solid electrolyte interphase (SEI) can also be formed during battery operation, which suppresses dendrite growth. Consequently, the Zn||Zn symmetric cell with GSH-modified electrolyte achieves an extended lifespan of up to 5000 h at −10 °C, 1700 h at 25 °C, and 2500 h at 40 °C. This work reveals a dynamic chemical self-deoxygenation/self-dehydrogenation strategy to design a non-corrosive and dendrite-free Zn anode from −10 to 40 °C for stable AZIBs.
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DOI
PubMed
高影响因子
Molecular Catalysis
·
2020
·
IF 3.687
Caixia Cui,Hong Ming,Linjing Li,Mingjie Li,Jian Gao,Tao Han,Yunyun Wang
We report here adopting a glucose template for synthesizing mesoporous silica for enzyme in-situ immobilization. Bifunctional GSH synthetase (GSHF) and polyphosphate kinase (PPK) were selected as model system for synthesizing GSH with ATP regeneration. Results indicated that a pore size that was slightly smaller than that of enzyme was responsible for the high performance of immobilized enzyme inside mesoporous without protein leaking. Then, the cationic dopamine was used for PPK immobilization by self-polymerization. The co-immobilized enzyme activity was much higher than that of the free enzyme. The catalytic efficiency kcat/Km of co-immobilized enzyme was higher than that of the free enzyme (8.5 and 1.5 times higher for GSHF and PPK, respectively). After optimizing the reaction parameters, the co-immobilized enzymes exhibited excellent operational and storage stability, and could be re-used seven times and retained the original activity after 30 days. It is hoped that these findings will pave the way for applying enzyme co-immobilization to biocatalytic system.
研究领域:
生物化学
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生物技术
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材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Meng-Xian Liu,Xiao Zhang,Wen-Ye Liu,Hui-Yong Wang,Yong-Liang Yu
Integrating long-lived room-temperature phosphorescence (RTP) into nanozymes to build multifunctional nanozymes can benefit biomedical analysis by expanding sensing modes and developing advanced sensing strategies but it is challenging. Herein, a general strategy for fabricating phosphorescent nanozymes by anchoring Co-N x active centers on SiO 2 nanospheres with carbon dots (CDs) encapsulated inside (CDs@SiO 2 @Co) is developed for dual-mode colorimetric-phosphorescent detection of glutathione (GSH). Specifically, surface Co-N x active centers enhanced O 2 adsorption and activation (O 2 to 1 O 2 ), providing oxidase-like activity to induce the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), generating a distinct colorimetric signal. The SiO 2 layer inhibited non-radiative transitions of the CDs to promote RTP, and spatially separated Co ions from CDs to prevent RTP quenching caused by Co-CD interactions, resulting in CDs@SiO 2 @Co with long-lived RTP (lifetime: 1.14 s), providing a phosphorescent channel free from autofluorescence interference. Upon introduction of GSH, the color product-oxidized TMB (oxTMB) was reduced, and the quenched RTP caused by the oxTMB internal filter effect was restored. Based on this principle, a sensitive and reliable dual-mode colorimetric-phosphorescent method was developed for detecting GSH in plasma and cells. Furthermore, owing to the tunable optical properties of CDs and the flexibility of substituting metal active centers, this strategy can be extended to construct various phosphorescent nanozymes with adjustable RTP emission wavelengths and diverse enzyme-like activities, advancing the development of nanozymes and bioanalytical platforms.
研究领域:
生物医学
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分析化学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 20
·9066-9072
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IF 3.591
Shaoguang Li,Huilin Liu,Xiaoying Zhang,Jiasong Cai,Kunlong Huang,Bing Chen,Liying Huang,Zhen Lin,Xinhua Lin,Hong Yao
A label-free, rapid, and highly sensitive colorimetric assay for the detection of glutathione (GSH) was developed. o-Phenylenediamine (OPD) can be selectively oxidized by Au3+ in phosphate buffer to produce light yellow 2,3-diaminophenazine. After the addition of GSH, the autocatalytic redox reaction between OPD and Au3+ can be effectively inhibited, resulting in a significant decrease in chroma and absorbance intensity. Reduced absorbance intensity can be used for the quantification of GSH. In addition, this method can achieve the discriminative detection of biothiols, such as cysteine and homocysteine. Based on these findings, a simple colorimetric assay was developed for the detection of GSH in the range of 1 nM to 1 mM with a low detection limit of 0.08 nM. More importantly, this procedure is simple, and the whole assay can be accomplished quickly within 15 min. Additionally, the proposed method was successfully used for the determination of GSH in biological fluids, such as serum samples.
研究领域:
分析化学
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生物医学
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DOI
高影响因子
ANALYTICAL LETTERS
·
2020
·
IF 1.467
Lanlan Wei,Xiaoting Lu,Xuejun Kang,Yuan Song
Exposure to heavy metals may affect a vast array of biochemical and nutritional processes in the human body. The toxic manifestations of these metals are caused primarily due to the imbalance between pro-oxidant and antioxidant homeostasis which is termed as oxidative stress. A high-performance liquid chromatography–chemiluminescence (HPLC-CL) method was developed for the simultaneous determination of two antioxidants, glutathione and cysteine, in human breast milk. The HPLC system included a C18 analytical column with a mobile phase composed of 1.0% methanol. The aminothiols were eluted from the C18 column, mixed with formaldehyde and potassium permanganate solutions, and subsequently monitored by a chemiluminescence detector. The new method was validated by monitoring glutathione and cysteine in human breast milk of volunteers from the Gusu and Industrial Districts of Suzhou, China. Four heavy metals in atmospheric particulate matter collected from the two districts were also determined by inductively coupled plasma–mass spectrometry (ICP-MS). The results demonstrated that the concentrations of cysteine and glutathione were significantly (p < 0.05) lower in human breast milk from the subjects living in the Gusu region of Suzhou, Jiangsu Province, China compared to an industrial district. These results were inversely correlated with the concentration levels of the toxic metals in collected atmospheric particulate matter. The decrease in the glutathione and cysteine concentrations in human breast milk appeared to be due to oxidative stress in the breast-feeding mothers.
研究领域:
分析化学
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环境科学
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医学
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2024
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IF 2
Shouxiang Jiang,Kai Fu,Xiangyu Sun,Xin Huang,Wenjun Xie,Chengfei Wang,Haoran Li,Xia Jiang,Yongjin Zou,Gengxiu Zheng,Guan Wang
The solubilities of glutathione in four binary solvent mixtures (water + acetone, water + 1,4-dioxane, water + acetonitrile, and water + tetrahydrofuran) were measured using a dynamic laser monitoring method at temperatures ranging from 278.15 to 318.15 K. The molar fraction solubility of glutathione in the binary solvent increased with increasing water content and temperature. The modified Apelblat, CNIBS/R-K, Van’t Hoff–Jouyban–Acree, Apelblat–Jouyban–Acree, and NRTL were applied to calculate the solubility of glutathione in these mixtures. The relative average deviation and root-mean-square deviation indicated a good correlation between the experimental and model solubility data. In addition, the KAT-LSER model was used to evaluate the solvent effect in the solvent–solvent and solvent–solute interactions. The results showed that the nonspecific dipolarity/polarizability interactions between the solvent and the solute were the main influencing factors, followed by the hydrogen bonding interactions between the solvent and the solute.
研究领域:
药物化学
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物理化学
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DOI
高影响因子
Results in Surfaces and Interfaces
·
2024
·
IF 0
Xiaohan Jiang,Junjie Zhang,Zitong Zhou,Zihao Zhou,Xinyi Cheng,Shaojun Shi,Jiexia Chen,Yunyun Ling
Blue fluorescent carbon dots with high purity were synthesized through a one-step hydrothermal method using citric acid and ethylenediamine as raw materials and then purification by column chromatography. In-situ controllable synthesis was used to prepare carbon dots-manganese dioxide (CDs-MnO 2 ) nanosheets, with carbon dots as the seed, potassium permanganate as the manganese source and sodium citrate as the reducing agent. The as-prepared nanosheets were characterized by transmission electron microscopy, absorption spectroscopy, and fluorescence spectroscopy. It was found that MnO 2 can be degraded by Glutathione (GSH) through a redox reaction, which restores the fluorescence of CDs. A new method for determining GSH was constructed using CDs-MnO 2 fluorescence sensing. The method is simple and sensitive, with linear ranges of 0.1–5 μM and 10–60 μM, respectively, and a lowest detection limit of 0.034 μM. The method was successfully used for the determination of GSH in human serum, with satisfactory recoveries in the range of 98.9–100.1%.
研究领域:
分析化学
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生物传感
·
纳米材料
✓ 已验证
DOI
iScience
·
2021
·
IF 5.458
Dao Lin,Yuhe Kan,Liang Yan,Yongqi Ke,Yang Zhang,Hang Luo,Xinjing Tang,Xiangjun Li,Yujian He,Li Wu
Summary The effective utility of physiologically active molecules is crucial in numerous biological processes. However, the regulation of enzyme functions through active substances remains challenging at present. Here, glutathione (GSH), produced in cells, was used to modulate the catalytic activity of thrombin without external stimulus. It was found that high concentrations of GSH was more conducive to initiate the cleavage of compound AzoDiTAB in the range of concentration used to mimic the difference between cancer and normal cells, which has practical implications for targeting cancel cells since GSH is overexpressed in cancer cells. Importantly, GSH treatment caused the deformation of G4 structure by cleaving AzoDiTAB and thus triggered the transition of thrombin from being free to be inhibited in complex biological systems. This work would open up a new route for the specific manipulation of enzyme-catalyzed systems in cancer cells.
研究领域:
生物化学
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分子生物学
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癌症研究
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DOI
PubMed
高影响因子
Current Drug Delivery
·
2024
·Vol. 22
·期 6
·784-797
·
IF 2.8
Mengqi Yi,Yangxin Lin,Yuyang Li,Bei Xiong,Yunhan Huang,Wei Guo,Bo Lu
BackgroundThe clinical efficiency of photodynamic therapy (PDT) in combination with chemotherapy has proven to be a promising strategy for tumor treatment, yet is restricted by the high glutathione (GSH) concentration at the tumor site and nonspecific drug targeting.ObjectiveThe goal of the current research was to create a biocompatible GSH-depleting and tumor-targeting nanoparticle (denoted as DOX/CA@PCN-224@HA) for the combined photodynamic and chemo photo-chemo) therapy.MethodsThe nanoparticles were characterized by transmission electron microscopy (TEM). A UV-vis spectrophotometer was used to measure the drug loading efficiency (DE) and encapsulation efficiency (EE). The GSH-depleting ability was measured using Ellman's test. Confocal laser scan microscopy (CLSM) was used to assess the cellular uptake. MTT was adopted to evaluate the cytotoxicity of DOX/CA@PCN-224@HA against 4T1 cells.ResultsThe altered PCN-224 showed excellent monodispersing with a dimension of approximately 193 nm ± 2 nm in length and 79 nm ± 3 nm in width. The larger and spindle grid-like structure of PCN-224 obtains better dual-drug loading ability (DOX: 20.58% ± 2.60%, CA: 21.81% ± 1.98%) compared with other spherical PCN-224 nanoparticles. The ultimate cumulative drug release rates with hyaluronidase (HAase) were 74% ± 1% (DOX) and 45% ± 2% (CA) after 72 h. DOX/CA@PCN-224@HA showed GSH-consuming capability, which could improve the PDT effect. The drug-loaded nanoparticles could accurately target 4T1 cells through biological evaluations. Moreover, the released DOX and CA display cooperative effects on 4T1 cells in vitro. DOX/CA@PCN-224@HA nanoparticles showed inhibition against 4T1 cells with an IC50 value of 2.71 μg mL-1.ConclusionThis nanosystem displays great potential for tumor-targeted enhanced (photo-chemo) therapy.
研究领域:
生物医学
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药理学
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肿瘤学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 9
·期 1
·187-194
·
IF 5.344
Yu Zhang,Hongyu Zhang,Pan He,Xuan Yi,Xinming Liu,Chunsheng Xiao
In this study, an enhanced anticancer strategy combining the chemotherapy from antineoplastics with the oxidative damage from a sulfur dioxide (SO2) prodrug is presented. Based on the characteristics of a high glutathione (GSH) level in the tumor microenvironment, a novel GSH-responsive SO2 polymeric prodrug mPEG-b-P(PA-alt-GDNs) was designed and synthesized via a ring-opening alternating copolymerization and “click” reaction. The GSH-sensitive mechanism of the polymer was investigated in detail. Furthermore, Irinotecan was loaded into the polymeric prodrug nanoparticles by a self-assembly method with a drug loading content of 12.3 wt% and a loading efficiency of 42.2%. The drug-loaded nanoparticles showed a sensitive response to high concentrations of GSH in the tumor cells and rapidly released both Irinotecan and SO2. The depletion of GSH and the release of SO2 were supposed to increase the level of reactive oxygen species in the tumor cell, which, in combination with the released Irinotecan, exerted an enhanced anti-proliferative effect against HepG2 cells. Finally, Irinotecan-loaded nanoparticles exhibited a stronger antitumor effect than free antineoplastics in HepG2 cells. Thus, these results indicated that our polymeric prodrug SO2 is a promising candidate for chemotherapeutic drug delivery and would be a new weapon in anticancer treatment.
研究领域:
药学
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肿瘤学
·
生物材料
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 3
·1-9
·
IF 5.833
Xian Zhiquan,Zhang Li,Yu Ying,Lin Bixia,Wang Yumin,Guo Manli,Cao Yujuan
A nanozyme based on CoFe 2 O 4 modified with MoS 2 was constructed for colorimetric determination of cysteine (Cys) and glutathione (GSH). Firstly, ferrite CoFe 2 O 4 is synthesized, and it is then modified by MoS 2 to form a flower-like polymer (MoS 2 @CoFe 2 O 4 ). In the presence of H 2 O 2 , a redox interaction takes place, and the resulting hydroxyl promoted a colorimetric conversion from colorless to blue in the presence of 3,3′,5,5′-tetramethylbenzidine (TMB). However, once Cys or GSH is added, they are capable to compete with the interaction of the hydroxyl with TMB, resulting in an inhibition of the colorimetric conversion. The colorimetric distinction is sensitive to the amount of target. The results obtained proved that the catalytic efficiency of MoS 2 @CoFe 2 O 4 is 4.4-fold and 1.8-fold to that of MoS 2 and CoFe 2 O 4 . Meanwhile, the K m values to TMB and H 2 O 2 are 0.067 and 0.048 mM, respectively, which are 6.5-fold and 77-fold, respectively smaller than those of natural peroxidase such as HPR. This indicates that the MoS 2 @CoFe 2 O 4 possesses a favorable interaction affinity. Additionally, the colorimetric distinction caused by the competition between TMB and cysteine or glutathione is obvious. The signal responses to cysteine and glutathione are linear in the range 0.5~15 μM and 0.5~35 μM, and the LODs are 0.10 and 0.21 μM, respectively. In practical assay of Cys in serum, the RSD of the sample tests is 4.6%, and the recoveries for the spiked assays are 95.3% and 96.0% with the RSD of 2.1% and 4.2%, respectively. Graphical abstract
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）