BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Wei-Jia Zeng,Ni Liao,Yan-Mei Lei,Jing Zhao,Ya-Qin Chai,Ruo Yuan,Ying Zhuo
In this work, hemin was firstly used as electrochemically regenerable co-reaction accelerator for signal amplification to develop an ultrasensitive aptasensor for Aflatoxin M1 (AFM1) detection. Initially, the perylenetetracarboxylic acid (PTCA) was directly employed as luminophore to construct the ECL sensing nano-platform by combining Au nanoparticles (Au NPs) for immobilizing thiol-terminated hairpin probe (H1). Then with the help of hairpin H2, H3, the AFM1-catalyzed hairpin assembly (CHA) was executed to produce the H1-H3 duplex, which could further initiate the hybridization chain reaction (HCR) to generate dendritic DNA polymers consisting of G-rich sequence for capturing large quantities of hemin on the electrode surface . Herein, hemin as electrochemically regenerable co-reaction accelerator could interact with the co-reactant (S 2 O 8 2− ) to obviously improve the luminous efficiency of the PTCA. Therefore, a strong and stable ECL signal was achieved by the employment of hemin as electrochemically regenerable co-reaction accelerator. The proposed aptasensor determined AFM1 down to 0.09 pg mL −1 within a linear range of 0.4 pg mL −1 to 400 ng mL −1 . With excellent sensitivity and stability, the strategy provided an efficient and simple method for the trace detection of biomolecules in clinical analysis.
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
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IF 3.232
Nuo Duan,Mofei Shen,Shuo Qi,Wenyue Wang,Shijia Wu,Zhouping Wang
An aptamer-based sensitive method was developed here for detection of multiple foodborne pathogens in food matrix by surface-enhanced Raman scattering (SERS) technology. Polydimethylsiloxane (PDMS) film was first prepared and then coated with gold nanoparticles (AuNP) to act as an active substrate for the enhancement of Raman scattering. The as-prepared Au-PDMS film was functionalized with specific pathogen aptamers (Apt) to capture the targets. In addition, aptamers functionalized AuNP integrated with Raman reporters (4-Mercaptobenzoic acid (4-MBA)/Nile blue A (NBA)) were fabricated as pathogen-specific SERS probes. In this scheme, pathogens were first captured by Apt-Au-PDMS film and then bind with SERS probes to allow the formation of a sandwich assay to complete the sensor module for the detection of multiple pathogens. With Vibrio parahaemolyticus and Salmonella typhimurium as model targets, this protocol can selectively detect 18 cfu/mL and 27 cfu/mL, respectively. Furthermore, this platform can be successfully applied to detect pathogens in seafood samples with recoveries ranging from 82.9% to 95.1%.
研究领域:
食品安全检测
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生物传感
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纳米技术
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 8
·2757-2766
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IF 4.478
Zhang Yanyan,Li Linze,Zhang Hao,Shang Junjian,Li Can,Naqvi Syed Muhammad Zaigham Abbas,Birech Zephania,Hu Jiandong
Abscisic acid (ABA), as the most common plant hormone in the growth of wheat, can greatly affect the yield when its levels deviate from normal. Therefore, highly sensitive and selective detection of this hormone is greatly needed. In this work, we developed an aptamer sensor based on surface-enhanced Raman spectroscopy (SERS) and applied it for the high sensitivity detection of ABA. Biotin-modified ABA aptamer complement chains were modified on ferrosoferric oxide magnetic nanoparticles (Fe 3 O 4 MNPs) and acted as capture probes, and sulfhydryl aptamer (SH-Apt)-modified silver-coated gold nanospheres (Au@Ag NPs) were used as signal probes. Through the recognition of the ABA aptamer and its complementary chains, an aptamer sensor based on SERS was constructed. As SERS internal standard molecules of 4-mercaptobenzoic acid (4-MBA) were encapsulated between the gold core and silver shell of the signal probes; the constructed aptamer sensor generated a strong SERS signal of 4-MBA after magnetic separation. When there were ABA molecules in the detection system, with the preferential binding of ABA aptamer and ABA molecule, the signal probes were released from the capture probes, after magnetic separation, leading to a linear decrease in SERS intensity of 4-MBA. Thus, the detection response was linear over a logarithmic concentration range, with an ultra-low detection limit of 0.67 fM. In addition, the practical use of this assay method was demonstrated in ABA detection from fresh wheat leaves, with a relative error (RE) of 5.43–8.94% when compared with results from enzyme-linked immunosorbent assay (ELISA). The low RE value proves that the aptamer sensor will be a promising method for ABA detection. Graphical abstract
研究领域:
植物学
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分析化学
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Jing Wen,Lijun Zhou,Ding Jiang,Xueling Shan,Wenchang Wang,Hiroshi Shiigi,Zhidong Chen
Herein, a sensitive and selective electrochemiluminescence (ECL) aptasensor was designed using [email protected] as accelerator towards the perylene derivative (PTC-Cys)/peroxydisulfate (S 2 O 8 2− ) system for kanamycin (KAN) assay. Firstly, the PTC-Cys was prepared by covalently binding l -cysteine to 3,4,9,10-perylenete-tracarboxylic acid, which was acted as the luminophore. Then [email protected] play the part of effective catalyst to accelerate the electrochemical reduction process of S 2 O 8 2− to produce more sulfate radical anions (SO 4 •- ), thus the ECL signal of the compound was noticeably raised by 2.4 times in comparison with that in which only luminophore and S 2 O 8 2− are present, achieving signal amplification of the ECL system. In the presence of KAN, aptamer was pulled down from the sensing interface, achieving a considerable enhancement of ECL intensity in S 2 O 8 2− solution. Upon the optimal condition, our proposed strategy can quantify the concentration of KAN from 1.0 × 10 −13 to 1.0 × 10 −8 M with low limit of detection of 4.2 × 10 −14 M (S/N = 3).Besides, our proposed ECL aptasensor exhibited excellent sensitivity, stability and specificity, and could be successfully applied to detect KAN in practical samples, which proved its potential to detect other antibiotics in food security.
研究领域:
分析化学
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生物传感
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食品安全
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Hong Yu,Chen Wang,Xinhe Xiong,Bitao Dai,Yang Wang,Zhijie Feng,Hao luo,Jiangxiong Zhu,Guoqing Shen,Yun Deng,Xueqing Geng,Danfeng Wang,Lumei Wang
Acetamiprid (Ace), extensively employed in agricultural production as a substitute for organophosphorus pesticides, poses a potential threat to the environment and human health due to excessive residues. Hence, the development of reliable and convenient techniques to determine Ace levels in the environment holds significant importance. Among various assay methods, nanomaterial-based aptamer-based biosensors (aptasensors) have emerged as efficient and promising tools. In this study, we successfully developed a novel aptasensor for monitoring Ace in the environmental water, utilizing advanced nanomaterials known as Fe-N-C single-atom nanozymes (SAzymes). Fe-N-C SAzymes exhibited excellent catalytic activity, high stability, and complete atom utilization. A facile dual-confinement route was employed for the synthesis of Fe-N-C SAzymes, and their performance as oxidase-like nanozymes was investigated. The synthesized Fe-N-C SAzymes presented distinct oxidase-like activity and demonstrated the capability to interact with a chromogenic indicator-TMB, serving as the signal transduction element in our assay. Furthermore, we found that thiol-modified aptamers could effectively dampen the oxidase-like activity of Fe-N-C SAzymes.Therefore, we successfully developed a Fe-N-C SAzymes assisted detection method for Ace, which exhibited high sensitivity (LOD = 16.9 nM) and selectivity. Overall, this work provides new insights into the design of multifunctional Fe-N-C SAzymes for environmental and biological applications.
研究领域:
环境科学
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生物技术
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纳米材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-11
·
IF 5.3
Du Juan,Li Zongshuang,Liu Kai,Guo Jiangli,Bai Yanhong
A novel colorimetric aptasensor assay based on the excellent magnetic responsiveness and oxidase-like activity of Fe 3 O 4 @MIL-100(Fe) was developed. Fe 3 O 4 @MIL-100(Fe) absorbed with aptamer and blocked by BSA served as capture probe for selective isolation and enrichment of Listeria monocytogenes one of the most common and dangerous foodborne pathogenic bacteria. The aptamer absorbed on Fe 3 O 4 @MIL-100(Fe) was further used as signal probe that specifically binds with target bacteria conjugation of capture probe for colorimetric detection of Listeria monocytogenes , taking advantages of its oxidase-like activity. The linear range of the detection of Listeria monocytogenes was from 10 2 to 10 7 CFU mL −1 , with the limit of detection as low as 14 CFU mL −1 . The approach also showed good feasibility for detection of Listeria monocytogenes in milk and meat samples. The spiked recoveries were in the range 81–114% with relative standard deviations ranging from 1.28 to 5.19%. Thus, this work provides an efficient, convenient, and practical tool for selective isolation and colorimetric detection of Listeria monocytogenes in food. Graphical Abstract
研究领域:
食品安全
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生物分析化学
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微生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Imran Mahmood Khan,Sen Zhao,Sobia Niazi,Ali Mohsin,Muhammad Shoaib,Nuo Duan,Shijia Wu,Zhouping Wang
T-2 is a trichthecenes mycotoxin responsible for severe food poisoning and toxic influences in humans and animals. Therefore, a rapid and sensitive detection of T-2 is an urgent need to protect the public health mainly caused by the consumption of contaminated agricultural products. Herein, we have prepared one pot synthesis of aptamer functionalized silver nanoclusters (apt-AgNCs) for fluorescent detection of T-2 that relies on novel fluorescence resonance energy transfer (FRET). Apt-AgNCs, act as energy donor, were applied as fluorescent sensing probe whose fluorescence was quenched by MoS 2 employed as fluorescent acceptor. Introduction of target in proposed bioassay leads to desorption of Apt-AgNCs from MoS 2 , and recovery of fluorescence in a toxin concentration dependent-manner. A dynamic quantification range was 0.005–500 ng mL −1 while the LOD was 0.93 pg mL −1 . Spiking analysis reveals the efficiency and robustness of the developed assembly, with recovery percentages ranged from 89.46% to 102.08% and 90.41% to 107.75% in maize and wheat samples, respectively. The results were cross-verified using standard commercial T-2 EISA kit. In conclusion, developed bioassay may find good utility in testing for risk assessment of T-2 toxin.
研究领域:
食品安全
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生物传感
·
毒理学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ze Chen,Zuyao Fu,Xiaoyu Du,Jing Xie,Zhaoyang Ding
Simple fluorescence aptasensors were established for malachite green (MG) and leucomalachite green (LMG) detection using G-quadruplex dyes thioflavin T (ThT) and thiazole orange (TO), respectively. ThT and TO, initially non-fluorescent in aqueous solution, exhibited fluorescence upon binding to target-specific aptamers, resulting in the stabilization of G-quadruplex structures. The specific binding between G-quadruplex and MG could induce conformational changes, which caused a pronounced quenching of fluorescence emitted by the intercalator, providing a possibility for detection. The developed assays demonstrated a good linear relationship in the range of 0–1000 μg L -1 for MG and 0–250 μg L -1 for LMG, with low limits of detection of 5.4 × 10 -3 μg L -1 and 0.1 μg L -1 respectively. More importantly, satisfactory detection results in water and fish samples were obtained. Noteworthy was that the uncomplicated fluorescent aptasensors operated effectively without the need for any modifications. The experimental process was facile and realized in homogeneous solution, which exhibited quick responsiveness after simple mixing.
研究领域:
生物化学
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分析化学
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食品安全
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Ya-Juan Yang,Ying Zhou,Yang Xing,Guo-Mei Zhang,Yan Zhang,Cai-Hong Zhang,Peng Lei,Chuan Dong,Xu Deng,Yujian He,Shao-min Shuang
In this study, a novel aptasensor based on Aptamer/NH 2 Janus particles is developed for the detection of Ochratoxin A(OTA). By coating gold on the hemispherical surface of the aminated polystyrene particles, Ochratoxin A aptamer is immobilized on the surface of the gold layer for selective identification and the other hemispherical able to bind to Glassy carbon electrode via peptide bond. Under optimum conditions, the sensor exhibited a wide dynamic range of OTA concentration from 1 × 10 −5 nM to 10 nM, and the detection limit is 3.3 × 10 −3 pM on condition of acceptable stability and reproducibility. The sensors were showed excellent performance in the detection of OTA in red wine sample with recoveries between 95.7% and 100.18%, which studied by the standard addition spiking technique. This work provides a new idea and method for preparing immune electrochemical sensors and is expected to be used for the OTA detection in red wine sample analysis.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Wanling Cui,Jinrong Liu,Wenqi Zhao,Jiayi Zhang,Yanyan Wang,Qiang Li,Rui Wang,Mei Qiao,Shicai Xu
Kanamycin residues accumulate in the human body and endanger human health, so the sensitive detection of kanamycin has important research significance. Herein, we constructed an enzyme-free and label-free fluorescent aptasensor for sensitive detection of kanamycin in milk samples based on hybridization chain reaction. We first designed a kanamycin recognition probe with a blocked aptamer and a blocked trigger sequence. Kanamycin specifically recognized the aptamer and then initiated the conformational transition process of the recognition probe to generate an aptamer-kanamycin complex with an activated trigger sequence. The trigger sequence initiated alternate hybridization of two partially complementary assembled probes to generate a long double-stranded DNA with many intact G-quadruplex structures for amplified, enzyme-free detection of kanamycin. The fluorescent dye N-methylmesoporphyrin IX was inserted into the intact G-quadruplex structures, and then it emitted a strong fluorescent signal for amplified, label-free detection of kanamycin. The linear range was from 2.0 to 200 nM for kanamycin, and the limit of detection (LOD), limit of quantification (LOQ) were 0.562 nM and 1.855 nM respectively. Moreover, the fluorescent aptasensor presented high selectivity for kanamycin compared with other antibiotics. Furthermore, the recoveries of spiked kanamycin in milk samples ranged from 98.66% to 100.7%, indicating that the fluorescent aptasensor had a great application prospect in food safety detection.
研究领域:
生物化学
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食品安全检测
·
分子生物学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Huixue Wang,Jinya Liu,Wei Chen,Jintong Na,Yong Huang,Guiyin Li
Glypican-3 (GPC3), a heparin sulfate proteoglycan, is a potential diagnostic and therapeutic target for hepatocellular carcinoma. In this paper, a novel fluorescent aptasensor for GPC3 detection is constructed via [email protected] quantum dots–labeled GPC3 aptamer ( [email protected] Apt ) as a fluorescence probe. First, [email protected] is screened out with higher fluorescence intensity, which emits bright blue fluorescence under ultraviolet light. Then, the fluorescence-labeled [email protected] Apt probe is formed by the combination of amination GPC3 Apt and [email protected] using EDC/NHS coupled reaction. Under hydrogen bond and π-π interaction/stacking, the fluorescence of [email protected] Apt could be quenched by reductive graphene oxide (RGO) with the help of the photoinduced electron transfer and the fluorescence resonance energy transfer mechanism. In the presence of GPC3, the [email protected] Apt specifically recognizes and binds to GPC3, giving rise to the change of secondary structure of GPC3 Apt to form the GPC3/GPC3 Apt [email protected] complex, which would lead to the disintegration of the [email protected] Apt -RGO compound. Therefore, the energy transfer process is blocked and the fluorescence intensity is restored, enabling a highly sensitive response to GPC3. When the concentration of GPC3 is from 5.0 ng/mL to 150.0 ng/mL, the fluorescence recovery rate is well linearly related to GPC3 concentration with the limit of detection of 2.395 ng/mL (S/N = 3). This strategy shows recoveries from 98.31% to 101.89% in human serum samples and provides simple, fast and cheap analysis of GPC3, which suggests that it has great potential applications in clinical diagnosis for hepatocellular carcinoma.
研究领域:
生物化学
·
分子诊断
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纳米医学
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产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Lei Chen,Shu Zhu,Xuepeng Wang
The detection of glioma cells (GMC) could be used to evaluate the effectiveness of treatment. Based on the advantages of easy synthesis and modification of aptamers and the advantages of high sensitivity, excellent selectivity, and wide application of electrochemical methods, this work constructs an aptamer electrochemical sensor that can be used for rapid and efficient detection of GMC. We used activators for the activation of carboxyl groups after modification of 4-carboxyphenyl diazonium salt. The aptamer, which can specifically recognize GMC, is immobilized on the electrode surface by forming an amide bond between the amino and carboxyl groups on the aptamer. The conditions affecting the sensing of the aptamer sensor were investigated in detail. The current of the aptamer sensor is proportionally with the GMC concentration from 1×10 7 to 1×10 2 cells/mL. Moreover, the sensor can identify GMC with reasonable specificity.
研究领域:
生物医学工程
·
肿瘤学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）