MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Xiaojing Si,Luchen Deng,Yingzi Wang,Mei Han,Yaping Ding
A novel poly alizarin red (AR) film incorporating carboxylic acid group functionalized multiwall carbon nanotube (f-MWCNT) modified glassy carbon electrode (GCE) was first prepared for the determination of luteolin (Lut). Scanning and transmission electron microscopy were applied for characterization of the surface morphology of the modified electrodes and cyclic voltammetry was used to investigate the electrochemical properties of the proposed electrode towards the oxidation of Lut. Optimization of the experimental parameters was performed with regard to pH, drop-coating volume of f-MWCNT, electro-polymerization cycles of alizarin red, and different potentials of i-t. And the mechanism for Lut detection based on AR/f-MWCNT/GCE was investigated by electrochemical methods. The linearity between the oxidation peak current and the Lut concentration was obtained in the range of 0.5 to 45 μM with a detection limit of 0.17 μM (S/N = 3). In addition, the AR/f-MWCNT/GCE electrode displayed high selectivity, good stability and reproducibility, and also successfully determined Lut in drug samples.
研究领域:
分析化学
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电化学
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纳米技术
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药物分析
✓ 已验证
产品关联
DOI
高影响因子
Evidence-based Complementary and Alternative Medicine
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2022
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IF 2.65
Ziqian Xu,Yihui Xie,Jun Song,Juntao Huang,Weimin Shi
Objective. This study aimed to explore the mechanisms of Baishi tablets (BSTs) in the treatment of vitiligo through network pharmacology-based identification and experimental validation. Methods. In brief, the compounds and related targets of BST were extracted from the TCMSP database, and disease information was obtained from the OMIM, GeneCards, PharmGkb, TTD, and DrugBank databases. A Venn diagram was generated to visualize the common targets of BST and vitiligo. GO and KEGG analyses were performed to explore the potential biological processes and signaling pathways. The PPI network and core gene subnetwork were constructed using STRING and Cytoscape software. In addition, the measurement of apoptosis in PIG1 cells and intracellular reactive oxygen species were measured using quercetin (QU), luteolin (LU), and kaempferol (KA) to protect melanocytes from oxidative stress. Results. A total of 55 compounds with 236 targets and 1205 vitiligo-related genes were obtained from the TCMSP database. GO and KEGG analyses were performed to explore the potential biological processes and signaling pathways, revealing that BST may cure vitiligo by influencing the biological processes of cellular oxidative stress and related signaling pathways. A critical subnetwork was obtained with 13 core genes by analyzing the PPI network, which includes HMOX1, CXCL8, CCL2, IL6, MAPK8, CASP3, PTGS2, AKT1, IL1B, MYC, TP53, IFNG, and IL2. Furthermore, a molecular docking analysis was conducted to simulate the combination of compounds and gene proteins, reflecting that QU, LU, and KA can strongly bind the core genes. Through a series of experimental validations, we found that QU, LU, and KA could attenuate H2O2-induced apoptosis in melanocytes. Further evidence revealed that QU, LU, and KA could enhance the scavenging of intracellular reactive oxygen species (ROS). Conclusion. Based on the results of network pharmacology analysis and experimental verification, QA, LU, and KA can be utilized to protect PIG1 cells by inhibiting oxidative stress and reducing the intracellular level of ROS. This may explain the underlying mechanism of BST therapy and provide a novel strategy for the treatment of vitiligo.
研究领域:
药理学
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皮肤科
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分子生物学
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中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2023
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IF 7
Zhaoying Wu,Rames Kaewmanee,Qianwen Yang,Zimin Wang,EN Xie,Jie Wei,Chao Zhang
Polymer-based composites possess some outstanding advantages over ceramic/metallic/polymeric biomaterials, which is considered as a promising candidate for bone repair. Tantalum (Ta)/polyimide (PI) biocomposites (PT) containing Ta nanoparticles of 20 v% (PT20) and 40 v% (PT40) were fabricated, and luteolin (LU) was loaded on PT40 (LUPT40). Compared with PT20 and PI, PT40 with high Ta content displayed high surface behaviors (e.g., roughness, surface energy, and hydrophilicity). PT40 remarkably improved the cell adhesion and multiplication, and LUPT40 with LU displayed further enhancement in vitro. Moreover, LUPT40 obviously boosted osteoblastic differentiation while suppressed the osteoclastic differentiation. Furthermore, LUPT40 exhibited good antibacterial effects because of the slow release of LU. The in vivo results confirmed that PT40 markedly promoted bone-formation, and LUPT40 further enhanced bone-formation/bone-bonding. In short, incorporation of Ta particles improved surface behaviors of PT40, which stimulated cell-response/bone-formation. Moreover, the slow release of LU from LUPT40 not only promoted cell-response/bone-formation but also enhanced bone-bonding. The synergistic effects of Ta and LU release from LUPT40 enhanced bone-formation/bone-bonding. Therefore, LUPT40 would have great potential for repair of bear-loading bone.
研究领域:
生物材料
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组织工程
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）