MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Lianjing Zhao,Dehao Kong,Zepei Wu,Guannan Liu,Yuan Gao,Xu Yan,Fangmeng Liu,Xiaomin Liu,Chenguang Wang,Jiuwei Cui,Geyu Lu
The combination of nanomaterials and DNA has captured extensive attention in the fields of sensor, catalysis, biomedicine and material science. The applications of DNA functionalized MoS 2 nanosheets (NSs) in biosensor and biomedicine in turn present an fascinating topic about biointerface science between DNA and MoS 2 NSs. The surface interaction between DNA and MoS 2 NSs was explored by adsorption and desorption experiments. The results indicated that van der waals force played an crucial role in DNA adsorption by MoS 2 NSs. Additionally, it was proved that the quenching of dye labelled DNA probe by MoS 2 NSs was attributed to fluorescence resonance energy transfer. Based on the extraordinarily high quenching efficiency of MoS 2 NSs and different adsorption affinity of MoS 2 NSs toward aptamer and aptamer-targeted protein, an aptameric biosensor was fabricated to detect carbohydrate antigen 15–3. The fabricated biosensor not only displayed high sensitivity with the detection limit of 0.0039 U/mL (S/N = 3), but also exhibited good stability and selectivity. The proposed sensing platform provided an easy-constructed and cost-effective protocol to detect other aptamer targeted biomolecules. The fundamental study of biointerface science on DNA and MoS 2 NSs will facilitate their potential applications in various fields.
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高影响因子
CATALYSIS LETTERS
·
2020
·Vol. 150
·期 9
·2607-2616
·
IF 2.482
Huang Benhua,Gao Ruixia,Pan Cheng,Fan Chao,Jia Xin,Pan Wanli,Li Xiaoyong,Li Heng,Sun Yang,Zhang Donghua
Abstract The atrolactic acid is an important intermediate for synthesis of non-steroidal anti-inflammatory pharmaceuticals, and meanwhile the traditional synthesis of atrolactic acid demands harsh conditions, lengthy steps, or big power consumption. In order to overcome these drawbacks, a series of Ce–Zr binary oxides are first developed as catalysts for oxidation of α-methylstyrene oxide into atrolactic acid. Catalytic results reveal these catalysts have high activity and recycling potential. This work shows a new method for preparation of atrolactic acid, which could be carried out under benign conditions, providing satisfactory outputs. Graphic Abstract
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 3
·1-10
·
IF 6.232
Guo Rui,Wang Dan-Ni,Wei Yun-Yun,Zhang Ying-Zhi,Yang Chun-Guang,Xu Zhang-Run
A controllable approach for preparing a portable colloidal photonic crystal (CPC) array chip is presented. The approach was inspired by the confinement effect of nanoparticle self-assembly on patterned surface. Hydrophobic polydimethylsiloxane substrate with reproducible micro-region array was fabricated by soft-lithography. The substrate was employed as the patterned template for self-assembly of monodisperse polystyrene nanoparticles. The CPC units can be prepared in several minutes, and exhibit consistent reflection wavelength. By adjusting the size of polystyrene nanoparticles and the shape of micro-regions, CPC units with multiple structure, colors and geometries were obtained. The CPC array chip features fluorescence enhancement owing to the optical modulation capability of the periodic nanostructure of the self-assembled CPC. With the reflection wavelength (523 nm) of green CPC units overlapping the emission wavelength (520 nm, with excitation wavelength of 490 nm) of 6-carboxyfluorescein-labeled DNA probe, the fluorescence intensity increased more than 10-fold. For signal-amplified assay of adenosine, the concentration range of linear response was 5.0 × 10 −5 mol L −1 to 1.0 × 10 −3 mol L −1 , and the limit of detection was 1.3 × 10 −6 mol L −1 . Because of the enhancement effect of photonic crystal, the fluorescence images were more readable from the CPC array chip, compared with those from the planar substrate. The chip has potential applications in multiplex determination with high-throughput via encoding strategy based on the tunable structure, color or geometric shape.
Schematic diagram of signal-enhanced fluorescent detection of adenosine based on the colloidal photonic crystal array chip (PDMS, polydimethylsiloxane; PS NPs, polystyrene nanoparticles; CPC, colloidal photonic crystal; GO, graphene oxide; FAM, 6-carboxyfluorescein).
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高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2020
·
IF 3.408
Si Chen,Feiyang Wu,Yan Hu,Shichao Lin,Chencheng Yu,Feng Zhu,Xu Wang
A novel fully bio-based intumescent flame retardant PA-GU was prepared by green and convenient ionic reaction of phytic acid (PA) and guanosine (GU) in aqueous environment, being prepared by adding PA-GU completely derived from renewable feedstocks to poly(butylene succinate) (PBS). The tests results showed that when the addition amount of PA-GU was 10 wt%, the peak of heat release rate (pHRR) of the sample decreased 59.2% (from 1413 kW/m 2 to 577 kW/m 2 ), the total heat release (THR) decreased 38.6% (from 132 MJ/m 2 to 81 MJ/m 2 ), and the average mass loss rate decreased to 11 g/m 2 •s (from 13 g/m 2 •s) compared to PBS containing no additive, all of which gradually decreased with the increasement of PA-GU. The preparation of flame retardant PBS composite not only means the expansion of the application of PBS, but also provides a new idea for the preparation of completely biologically derived flame-retardant materials.
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高影响因子
MOLECULES
·
2019
·Vol. 24
·期 22
·4119
·
IF 3.06
Zhonghua Dong,Yueyue Sun,Guangwei Wei,Siying Li,Zhongxi Zhao
Cordyceps Sinensis, a traditional Chinese medicine and a healthy food, has been used for the treatment of kidney disease for a long time. The aim of present study was to isolate a nucleoside/nucleobase-rich extract fromCordyceps Sinensis(CS-N), determine the contents of nucleosides and nucleobases, and explore its anti-diabetic nephropathy activity. CS-N was isolated and purified by using microporous resin and glucan columns and the unknown compounds were identified by using HPLC-DAD and LC-MS. The effects of CS-N on the epithelial–mesenchymal transition (EMT), extracellular matrix (ECM) depositions, and the MAPK signaling pathway were evaluated in streptozotocin (STZ)-induced diabetic mice and high glucose (HG)-exposed HK-2 cells. CS-N significantly attenuated the abnormity of renal functional parameters, ameliorated histopathological changes, and inhibited EMT and ECM accumulation by regulating p38/ERK signaling pathways. Our findings indicate that CS-N exerts a therapeutic effect on experimental diabetic renal fibrosis by mitigating the EMT and the subsequent ECM deposition with inhibition of p38 and ERK signaling pathways.Keywords:nucleosides and nucleobases;diabetic nephropathy;HK-2 cells;extracellular matrix;epithelial–mesenchymal transition
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-9
·
IF 5.479
Hou Xingyu,Huang Wei,Tong Yukui,Tian Miaomiao
The authors describe boronate-modified hollow dummy template imprinted polymers (B-hDIPs) for selective and sensitive extraction of the catecholamines norepinephrine (NE), epinephrine (E), and dopamine (DA) from urine samples prior to their detection by HPLC with UV detector. The B-hDIPs were prepared by using (a) nano-TiO 2 as the sacrificial support, (b) 3-carboxyphenylboronic acid (CPBA) as the boronate-affinity functional monomer, and (c) catechol which acts as dummy template. The nano-TiO 2 can be etched off by using hydrofluoric acid to form the hollow imprint. The main advantages of using such hollow polymers arise from their controllable hole structure which favors template removal and fast mass transfer. The B-hDIPs material was characterized by FT-IR, transmission electron microscope, dynamic light scattering, X-ray photoelectron spectrum, and thermal gravimetric analysis. The effects of imprinting conditions, sample pH, sample flow rate, adsorbent amount, selectivity and competitive capacities were investigated. Under optimal conditions, the limits of detection for the catecholamines are in the range from 15 to 47 ng mL −1 . The method was successfully applied to their determination of NE, E, and DA in spiked urine, with recoveries ranging from 63.4 to 106.2%.
Schematic presentation of boronate-modified hollow dummy template imprinted polymers as an adsorbent to extraction of the catecholamines (norepinephrine, epinephrine, and dopamine) from urine samples prior to their detection by HPLC-UV.
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高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2019
·
IF 3.372
Tengda Wang,Jianxing Shan,Li Wang,Xiangwen Zhang,Guozhu Li
Aqueous-phase hydrogenation of 4-nitrophenol (4-NIP) by H 2 has been investigated in the presence of Pd nanoparticles (NPs) regulated by small biological molecules. Nucleosides and their derivatives were employed as capping agents of Pd NPs, and their effect on Pd activity was evaluated. All four kinds of nucleosides exhibited obvious promoting effect on the activity of Pd NPs in comparison to that without any capping agent. The general promoting effect sequence of nucleosides and their derivatives on the activity of Pd NPs was nucleoside ≥ nucleotide > deoxynucleoside > base > deoxynucleotide. The quantitative correlation between the activity promoting effect and the structure of nucleosides and their derivatives were also studied using the method based on poly-parameter linear free energy relationships. Moreover, the kinetic behaviors of 4-NIP hydrogenation over Pd NPs regulated by different nucleosides (adenosine, guanosine, cytidine, and β-thymidine) were studied systematically and different kinetic models were developed to fit the data. The obtained kinetic data fitted well to the Langmuir-Hinshelwood (L-H) model, where the surface reaction is the rate-controlling step. The apparent activation energy, adsorption enthalpy of 4-NIP, and dissociated adsorption enthalpy of hydrogen were calculated and compared for a better understanding of the mechanism. Pd NPs regulated by cytidine showed the lowest apparent activation energy of 43.66 kJ mol −1 .
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Maofang He,Rong Wang,Yijia He,Jiwei Shen,Chunye Liu,Chaozhan Wang,Yinmao Wei
Boronate affinity is an important method for the enrichment and separation of cis -diol containing compounds, but most of the conventional boronate materials suffer from weak binding strength as well as low binding capacity towards glycoproteins due to the use of single boronic acids as ligands. In this work, a novel multidentate boronate magnetic adsorbent was assembled by using amined polyhedral oligomeric silsesquioxane as spacer and a diboronic acid as ligand. The specially designed adsorbent exhibited high adsorption capacity for cis -diols due to the high density of phenylbronic acid moieties. More interestingly, the dissociation constants toward glycoproteins on the material were lowered to be ∼10 −6 M, being at least 3 orders lower than the single boronic acid bonded adsorbents. By comparing the binding properties of small molecules containing one and two pairs of cis -diols, the enhanced binding strength of glycoproteins on the multidentate boronate magnetic adsorbent was attributed to the synergistic binding of glycoproteins on the special interface. The new materials successfully captured glycoproteins from 1000-fold diluted egg white, suggesting that the material could be an optional alternative adsorbent for enriching trace glycoproteins from complex bio-samples.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-8
·
IF 5.479
Wang Jing,Li Bingzhi,Lu Qiaoyun,Li Xiaoyun,Weng Chenyuan,Yan Xiaoqiang,Hong Junli,Zhou Xuemin
The authors describe a versatile aptasensing scheme based on the use of polypyrrole nanoparticles (PPyNPs) and DNA-silver nanoclusters (DNA-AgNCs) for multiple target detection. The DNA-AgNCs consist of two functional domains, viz. (a) a nucleation domain for attaching the metal core of the nanoclusters, and (b) a recognition domain which consists of a single-stranded aptamer. In the absence of analytes, the single-strand recognition domain will be absorbed onto the surface of the PPyNPs through π stacking and hydrophobic interactions. As a result, the red fluorescence of the DNA-AgNCs (with excitation/emission peaks at 535/625 nm) is quenched by the PPyNPs. On introducing the analytes, the DNA-AgNCs will bind them. This leads to the desorption of DNA-AgNCs and the recovery of the red fluorescence. Based on the above strategy, a versatile, sensitive and selective aptasensor was established for detection of adenosine, thrombin and interferon-gamma. The method was applied to the detection of the above targets in (spiked) serum samples and gave satisfactory results, with detection limit of 0.58 nM for IFN-γ, 0.39 nM for adenosine, and 2.2 nM for thrombin. The use of PPyNPs results in uniquely low non-specific absorption and in improved analytical results in case of real-sample analysis when compared to previously reported methods.
Schematic illustration of DNA-silver nanoclusters and polypyrrole nanoparticles in an aptasensor for detection of multiple targets.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 15
·2523-2533
·
IF 2.516
Yuan Yuan,Min Jiang,Hongyang Zhang,Ju Liu,Min Zhang,Ping Hu
We present an ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole time-of-flight mass spectrometry method for the simultaneous quantification of ten purines (adenine, hypoxanthine, guanine, xanthine, deoxyadenosine, adenosine, inosine, guanosine, xanthosine, and uric acid) and creatinine in human urine. After chromatographic separation on an ACE Excel 2 AQ column, high abundant creatinine and uric acid and the other low abundant purines were sequentially detected by ultraviolet and quadrupole time-of-flight mass spectrometry within a single run. Method validations including specificity (improved by accurate mass measurement), linearity (correlation coefficients ≥0.9944), limit of quantification (0.002–9.756 µg/mL), intra- and interday precision (relative standard deviations ≤9.1 and 14.0%, respectively), accuracy (relative errors ≤13.1%), extraction recovery (between 90.3 and 109.6%), matrix effect (between 85.3 and 110.5%), and stability (relative errors ≤14.3%) were fully evaluated. This approach was applied to characterize the disordered purine metabolism in acute and chronic gout as an example. Quantitative results (normalized by creatinine) showed that an overproduction of urinary purine precursors might be involved in the gout process. The developed method represents a useful tool to investigate the purine disturbances in gout and other relevant diseases.
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JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Yu-Mei Zhao,Lv-Huan Wang,Si-Fan Luo,Qi-Qin Wang,Ruin Moaddel,Ting-Ting Zhang,Zheng-Jin Jiang
Neuraminidase (NA) is a glycoside hydrolase that has been proposed as a potential therapeutic target for influenza. Thus, the identification of compounds that modulate NA activity could be of great therapeutic importance. The aim of this study is to develop a drug discovery tool for the identification of novel modulators of NA from both compound libraries and natural plant extracts. NA was immobilized onto the surface of magnetic beads and the inherent catalytic activity of NA-functionalized magnetic beads was characterized. Based on the enzymatic activity (hydrolysis ratio), the inhibitory activities of 12 compounds from plant secondary metabolites were screened, and the desired anti-NA activities of flavonoids were certified. Ligand fishing with the immobilized enzyme was optimized using an artificial test mixture consisting of oseltamivir, lycorine and matrine prior to carrying out the proof-of-concept experiment with the crude extract of Flos Lonicerae . The combination of ligand fishing and HPLC-MS/MS identified luteolin-7- O -β-D-glucoside, luteolin, 3,5-di- O -caffeoylquinic acid and 3,4-di- O -caffeoylquinic acid as neuraminidase inhibitory ligands in Flos Lonicerae . This is the first report on the use of neuraminidase functionalized magnetic beads for the identification of active ligands from a botanical matrix, and it sets the basis for the de novo identification of NA modulators from complex biological mixtures.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Rong Wang,Zhenkun Mao,Zilin Chen
A novel monolithic column based on the copolymerization of ethylene dimethacrylate and a deep eutectic solvent (DES), which composed of chlorocholine chloride and itaconic acid, was developed for capillary electrochromatography (CEC). The structure and morphology of the obtained monolithic column was characterized by Fourier transform infrared spectra and scanning electron microscopy, which showed a porous monolithic structure with good permeability. The influence of pH value and acetonitrile content on electroosmotic flow (EOF) of DES-based monolithic column was investigated. The DES-based monolithic column was used as the separation column of CEC and exhibited excellent performance for the separation of neutral compounds, phenols, toluidines, nucleosides, nucleotide bases and alkaloids. The retention mechanism might be attributed to synergistic effect of hydrophobic interaction, hydrogen bond interaction and electronic interaction. The DES-based monolithic column showed good repeatability with relative standard deviations of migration time less than 0.80%, 2.15%, 4.26% and 3.08% for intra-day, inter-day, column-to-column and batch-to-batch, respectively. Thus, organic polymer monolithic columns with DESs as functional monomers are a promising option in chromatographic separation.
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高影响因子
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