Frontiers in Microbiology
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2022
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IF 6.064
Cai Xiao-Yu,Xu Man,Zhu Yu-Xuan,Shi Ying,Wang Hong-Wei
Neonicotinoids (NEOs) are the most widely used insecticides in the world and pose a serious threat to aquatic ecosystems. The combined use of free-floating aquatic plants and associated microorganisms has a tremendous potential for remediating water contaminated by pesticides. The aim of this study was to determine whether plant growth-promoting bacteria (PGPB) could enhance the phytoremediation efficiency of duckweed (Lemna aequinoctialis) in NEO-contaminated water. A total of 18 different bacteria were isolated from pesticide-stressed agricultural soil. One of the isolates, Pseudomonas monteilii FC02, exhibited an excellent ability to promote duckweed growth and was selected for the NEO removal experiment. The influence of strain FC02 inoculation on the accumulation of three typical NEOs (dinotefuran, thiacloprid, and imidaclothiz) in plant tissues, the removal efficiency in water, and plant growth parameters were evaluated during the 14-day experimental period. The results showed that strain FC02 inoculation significantly (p < 0.05) increased plant biomass production and NEO accumulation in plant tissues. The maximum NEO removal efficiencies were observed in the inoculated duckweed treatment after 14 days, with 92.23, 87.75, and 96.42% for dinotefuran, thiacloprid, and imidaclothiz, respectively. This study offers a novel view on the bioremediation of NEOs in aquatic environments by a PGPB–duckweed partnership.
研究领域:
环境科学
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微生物学
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植物学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
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2020
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IF 1.467
Xu Ning,Liu Mu-Hua,Yuan Hai-Chao,Huang Shuang-Gen,Wang Xiao,Zhao Jin-Hui,Chen Jian,Wang Ting,Hu Wei,Song Yi-Xin
In order to meet the growing demand for food safety, the rapid classification of sulfadimidine and sulfapyridine in duck meat based on surface-enhanced Raman spectroscopy (SERS) was investigated using gold nanoparticles (Au NPs) and NaCl solution as the active substrate and active agent, respectively. The results showed that the Raman characteristic peaks at 557 and 573 cm−1 were used to determine whether sulfadimidine and sulfapyridine remained in duck meat. A single factor experimental method was used to optimize the conditions. Three pretreatment methods were employed to optimize the raw measurements. According to the classification accuracies, adaptive iterative reweighted penalty least squares and second derivative were selected to be the optimal pretreatment method. Next, principal component analysis (PCA) was performed to extract the characteristic variables. The first four score values of PCA were selected to be the input values of support vector machine (SVM) classification model, which classified duck meat samples into four categories. Nu-support vector classification and radial basis function were selected to be the employed SVM classification model and the Kernel type, respectively. The gamma and nu values were 0.25 and 0.255, respectively. The classification accuracy for the test set was 90.44%. The sensitivities and specificities of test set were calculated, and the highest sensitivity and specificity values were 96.97% and 100%, respectively. The experimental results showed that this classification model provides favorable classification results. Therefore, the adopted method may be used for the rapid classification of sulfadimidine and sulfapyridine residues in duck meat.
研究领域:
食品安全
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光谱学
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生物医学工程
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DOI
高影响因子
Food Science and Human Wellness
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2020
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IF 2.455
Wenfu Hou,Qiqi Yue,Wen Liu,Ji Wu,Yang Yi,Hongxun Wang
Chilled duck meat is a popularly consumed meat in China but easily perishes at room/low temperature. Kojic acid is usually used as an antimicrobial. This study investigated the sensory scores, total bacteria number, and total volatile base nitrogen (TVB-N) value of chilled duck meat under kojic acid treatment during storage. The results presented that kojic acid suppressed the growth of spoilage bacteria and prolonged the shelf-life of chilled duck meat by at least two days. In further investigation, the bacterial community composition was explored through high-throughput sequencing. Pseudomonas spp. were the predominant spoilage bacteria after 6-day storage and were significantly suppressed by kojic acid. Besides, Brochothrix showed drug resistance and became the dominant bacteria in the kojic acid treated sample after 6-day storage. In conclusion, kojic acid maintained the sensorial, chemical, and microbial quality of duck meat stored at low temperatures. Meanwhile, kojic acid enriched the bacteria composition and showed strong antimicrobial activity against Pseudomonas spp.
研究领域:
食品科学
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微生物学
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生物技术
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产品关联
DOI
高影响因子
BMC Veterinary Research
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2014
·Vol. 10
·期 1
·1-9
·
IF 1.743
Xiong Wen,Chen Yun,Wang Yu,Liu Jiaguo
Background Duck viral hepatitis (DVH) is an acute disease of young ducklings with few convenient and effective veterinary drugs to treat. In pathology, present study mainly focused on the immune mechanism, but very few studies have concerned with the role of oxidative stress in the pathogenesis of DVH. To study the antioxidative and hepatoprotective effects of icariin and its phosphorylated derivative against DVH, we prepared phosphorylated icariin (p-icariin) using the sodium trimetaphosphate–sodium tripolyphosphate method. Ducklings were drunk with icariin and p-icariin after being challenged with duck hepatitis virus 1 (DHV-1). We recorded the number of dead ducklings, gross pathological changes in the liver, and changes in indices of oxidative stress and liver injury. The correlations between these indices were also analyzed.Results Exposure to DHV-1 induced significant oxidative damage in ducklings. Administration of icariin or p-icariin attenuated liver pathological injury and significantly increased the survival rate, with better outcomes in ducklings treated with p-icariin than in those treated with icariin. Icariin and p-icariin also attenuated the changes in oxidative stress and liver injury. We found positive correlations among indices of oxidative stress (malondialdehyde and inducible nitric oxide synthase) and liver injury (alanine aminotransferase, alkaline phosphatase, and lactate dehydrogenase), suggesting that DHV-1 causes significant oxidative damage, which is related to the extent of hepatic injury.Conclusion sIcariin and p-icariin improved the survival and attenuated oxidative stress and liver dysfunction induced by DHV-1. These outcomes were better in ducklings treated with p-icariin than in those treated by icariin. The clinical effects of both components were related to their antioxidant activities.
研究领域:
兽医学
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药理学
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病毒学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
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IF 3.671
Ke Ming,Yun Chen,Jintong Shi,Jingjing Yang,Fangke Yao,Hongu Du,Wei Zhang,Jingying Bai,Jiaguo Liu,Deyun Wang,Yuanliang Hu,Yi Wu
To explore new effective anti-duck hepatitis A virus drugs, Chrysanthemum indicum polysaccharide (CIPS) was phosphorylation modified using STMP-STPP method, and phosphorylated Chrysanthemum indicum polysaccharide (pCIPS) was obtained. Characteristic absorption peaks were observed in pCIPS using IR spectrum, suggested that CIPS was successfully modified. In addition, field emission scanning electron micro-scope (FE-SEM) was used to observe the polysaccharides’ surface features. In vitro, we found that the survival rate of DHAV-infected hepatocytes increased after the two drugs treatment, indicated that the two drugs possess good anti-DHAV activity. The results of real-time PCR showed that pCIPS inhibited the virus gene replication more effectively than CIPS. Reed-Muench assay was used to observe the changes of the virulence, and the expression level of IFN-β was observed to verify the changes of virulence. In vivo experiment, the blood virus content reduced after CIPS and pCIPS treatment. To evaluate the ducklings’ hepatic injury, the serum ALT, AST, TP and ALB levels were detected. Results showed that both CIPS and pCIPS could alleviate the hepatic injury of ducklings infected DHAV, especially for pCIPS. All the results above mentioned demonstrated that the anti-DHAV activity of CIPS was enhanced after phosphorylation modification.
研究领域:
药理学
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病毒学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Tianxin Qiu,Yu Shi,Miao He,Wenjia Wang,Jinwu Meng,Jinxue Ding,Weiran Wang,Siya Li,Kun Li,Jiaguo Liu
In order to ascertain the mechanism underlying the therapeutic efficacy of Bush sophora root polysaccharides (BSRPS) and phosphorylated Bush sophora root polysaccharides (pBSRPS) in the treatment of in duck viral hepatitis (DVH), an investigation was conducted to assess the protective impact of BSRPS and pBSRPS against duck hepatitis A virus type 1 (DHAV-1) induced mitochondrial dysfunction both in vivo and vitro . The BSRPS underwent modification through the utilization of the sodium trimetaphosphate - sodium tripolyphosphate method, and was subsequently characterized though Fourier infrared spectroscopy and scanning electron microscopy. Following this, the degree of mitochondrial oxidative damage and dysfunction was described through the use of fluorescence probes and various antioxidative enzyme assay kits. Furthermore, the utilization of transmission electron microscopy facilitated the observation of alterations in the mitochondrial ultrastructure within the liver tissue. Our findings demonstrated that both BSRPS and pBSRPS effectively mitigated mitochondrial oxidative stress and conserved mitochondrial functionality, as evidenced by heightened antioxidant enzyme activity, augmented ATP production, and stabilized mitochondrial membrane potential. Meanwhile, the histological and biochemical examinations revealed that the administration of BSRPS and pBSRPS resulted in a reduction of focal necrosis and infiltration of inflammatory cells, thereby mitigating liver injury. Additionally, both BSRPS and pBSRPS exhibited the ability to maintain liver mitochondrial membrane integrity and enhance the survival rate of ducklings infected with DHAV-1. Notably, pBSRPS demonstrated superior performance in all aspects of mitochondrial function compared to BSRPS. The findings indicated that maintaining mitochondrial homeostasis is a crucial factor in DHAV-1 infections, and the administration of BSRPS and pBSRPS may mitigate mitochondrial dysfunction and safeguard liver function.
研究领域:
病毒学
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药理学
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分子生物学
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病理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Mengge Xiao,Andrew Yuk Keung Leung,Zhonghao Zhu,Liang Zhu,Kaijun Xiao
In this paper, response surface methodology (RSM) was used to optimize the extraction process of proteins from duckweed and the protein extraction rate was increased to 24.11 ± 0.04 %. The duckweed protein extract (DP) was mixed with sodium carboxymethyl cellulose (CMC) and glycerol to prepare DP/CMC antioxidant membranes with different additions (0.5 % ∼ 2.5 %), and 2.0 % DP/CMC antioxidant membranes were used in blueberry preservation experiments. The results showed that the moisture permeability of DP/CMC antioxidant membrane was significantly reduced, the thermal stability was significantly improved, and the antioxidant property was significantly higher than that of CMC membrane ( p < 0.05). The lowest oxygen content in the 2.0 % DP/CMC membrane package compared to PE and CMC membranes may be due to the synergistic effect of aerobic respiration of blueberries and oxidation of antioxidants in the membrane, under which the loss of antioxidants in the blueberries was lowest and the quality of blueberries was effectively maintained. In this study, for the first time, antioxidant membranes with significant freshness preservation effects were prepared using duckweed protein extract (DP), demonstrating its great potential as an active packaging material for food preservation, and opening up a new development direction for the application of DP.
研究领域:
食品科学
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生物技术
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植物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Bojia Li,Hongyu Liang,Yueqi Hu,Xinyue Zhou,Peiyu Yang,Xiaojuan Chen,Hongshan Liang,Can Wu,Bin Zhou
Tannic acid (TA) has attracted the attention of researchers as a promising organic ligand capable of forming metal-organic coordination networks with various metal ions at interfaces to impact surface properties. In this study, we innovatively reported a self-assembly method for surface decoration by depositing TA/Fe 3+ coatings on the surface of desalted duck egg white nanoparticles (DEWN), further studying the oil/water interfacial properties of the modified particles. The results showed that the ratio and concentration of TA to Fe 3+ could modulate interfacial properties. The modified DEWN has low interfacial tension, with TFe2 having near-neutral wettability (θ o/w ~ 90°) and stabilizing emulsions for over 60 days. Moreover, the emulsions stabilized by TFe1 and TFe2 formed stronger gel structures with better thixotropic recovery (98.82 % and 89.26 %). After further increasing the oil phase ratio, the increased layer assembly concentration improved the stability of the oil phase and formed a dense gel mesh structure. The effects of temperature and salt ion concentration on the emulsion were investigated under optimum conditions, both of which showed good stability. Overall, our research not only highlighted straightforward strategies for preparing emulsions with higher stability using green and sustainable raw materials, but also broadened the range of applications for metal-phenol decoration.
研究领域:
食品科学
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材料化学
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胶体与表面化学
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产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
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2018
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IF 2.129
Zhaopeng Wang,Xuezhi Yu,Licai Ma,Hebing Liu,Shuangyang Ding,Zhanhui Wang,Xiya Zhang,Jianzhong Shen,Kai Wen
In this work, high affinity polyclonal antibodies for ribavirin (RBV) from new haptens were prepared and were used to analyse RBV residues in chicken muscle, eggs and duck muscle. The new haptens were synthesised with different spacers, and the best antibody was obtained with an IC50 value as low as 0.61 ng/mL in indirect competitive enzyme-linked immunosorbent assay (ELISA). The cross-reactivities with another five antiviral drugs including amantadine, rimantadine, moroxydine, zanamivir and oseltamivir were less than 0.1%, which indicated the good specificity of the antibody. An ELISA was developed based on the antibody and applied to detect RBV in multi-food matrices. The sample preparation prior to detection only needed simple dilution after trichloroacetic acid extraction. The limits of detection were 1.07, 1.18 and 1.03 μg/kg in chicken muscle, eggs and duck muscle, respectively. Recoveries ranged from 89.0% to 112.7% with coefficients of variation below 13.0%. Ten blind samples of chicken muscle were analysed simultaneously by ELISA and liquid chromatography-tandem mass spectrometry, and a good correlation between the methods was observed. The results indicated that the high affinity antibody could be applied for the simple and fast detection of RBV in multi-food matrices.
研究领域:
免疫学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
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2022
·
IF 4.336
Xueru Wang,Ruiming Hu,Chang Wang,Zejing Wei,Shaoxing Pi,Yong Li,Guyue Li,Fan Yang,Caiying Zhang
Excessive molybdenum (Mo) and cadmium (Cd) have toxic effects on animals. However, hepatotoxicity co-induced by Mo and Cd in ducks is still unclear. To evaluate the effects of Cd and Mo co-exposure on autophagy by nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated antioxidant defense and endoplasmic reticulum stress (ERS) in duck livers, 40 healthy 7-day-old ducks were randomly assigned to 4 groups and fed diets containing different doses of Mo and/or Cd for 16 weeks, respectively. The results verified that Mo and/or Cd induced oxidative stress via decreasing glutathione peroxidase (GSH-Px), catalase (CAT), and total-superoxide dismutase (T-SOD) activities and increasing hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) concentrations; inhibited Nrf2 axis by downregulating the pathway-related genes and proteins expression levels , and activated ERS through upregulating the protein kinase RNA-like endoplasmic reticulum kinase (PERK)/eukaryotic initiation factor 2a (eIF2a), inositol-requiring enzyme 1 (IRE1) and activating transcription factor 6 (ATF6) pathway-related genes and proteins expression levels, which triggered autophagy via increasing autophagosomes , light chain 3 (LC3) puncta, LC3A, LC3B, autophagy-related gene 5 (Atg5), Bcl-2-interacting protein (Beclin-1) mRNA levels and Beclin-1, microtubule-associated protein light chain 3 II/I (LC3II/LC3I) protein levels, decreasing Dynein, p62 , mammalian target of rapamycin (mTOR) mRNA levels and p62 protein level. Additionally, the changes in Mo and Cd group were the most obvious. Briefly, our study reveals that autophagy induced by Mo and/or Cd may be associated with the activation of crosstalk between Nrf2-mediated antioxidant defense response and ERS in duck livers. Mo and Cd may aggravate toxic damage to the liver.
研究领域:
毒理学
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分子生物学
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兽医学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
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2025
·Vol. 15
·期 5
·286
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IF 5.6
Junshi Huang,Runhua Zhou,Jinlong Lin,Qi Chen,Ping Liu,Shuanggen Huang,Jinhui Zhao
Sulfadimethazine (SM2) is widely used in livestock and poultry farming, but its improper use can pose a serious threat to human health. Therefore, the detection of SM2 residues in livestock and poultry products, including duck blood, is of great significance for food safety. A rapid detection method for SM2 residues in duck blood based on surface-enhanced Raman spectroscopy (SERS) was proposed in this paper. Density functional theory (DFT) was employed to optimize the molecular structure of SM2 and perform theoretical Raman vibrational analysis, thereby identifying its characteristic peaks. The enhancement effects of four different substrates were compared. The sample pretreatment method and detection conditions were optimized through single-factor experiments, including the types and amounts of electrolyte aggregators, the amount of gold nanocolloids, and the adsorption time. Under optimal conditions, the SERS spectral data of the samples were preprocessed, and features were extracted to establish an optimal quantitative prediction model. The experimental results found that the adaptive iteratively reweighted penalized least-squares method (air-PLS) was the best preprocessing method, and the competitive adaptive reweighted sampling–multiple linear regression (CARS-MLR) model demonstrated the best prediction performance, with a coefficient of determination for the prediction set (Rp2) of 0.9817, a root mean square error of calibration (RMSEC) of 1.5539 mg/L, a relative prediction deviation (RPD) of 7.1953, and limits of quantification of 0.75 mg/L. The research demonstrated that the combination of SERS technology and chemometric methods was feasible and effective for the detection of SM2 residues in duck blood.
研究领域:
食品安全
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分析化学
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药物残留检测
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产品关联
DOI
PubMed
高影响因子
EXPERIMENTAL BIOLOGY AND MEDICINE
·
2016
·
IF 2.542
Yixuan Wang,Yun Chen,Hongxu Du,Jingjing Yang,Ke Ming,Meiyun Song,Jiaguo Liu
Duck hepatitis A virus (DHAV) (Picornaviridae) causes an infectious disease in ducks which results in severe losses in duck industry. However, the proper antiviral supportive drugs for this disease have not been discovered. Polysaccharide is the main ingredient of Astragalus that has been demonstrated to directly and indirectly inhibit RNA of viruses replication. In this study, the antiviral activities of Astragalus polysaccharide (APS) and its derivatives against DHAV were evaluated and compared. APS was modified via the sodium trimetaphosphate and sodium tripolyphosphate (STMP-STPP) method and chlorosulfonic acid-pyridine method to obtain its phosphate (pAPS) and sulfate (sAPS), respectively. The infrared structures of APS, pAPS, and sAPS were analyzed with the potassium bromide disc method. Additionally, the antiviral activities were evaluated with the MTT ((4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) method in vitro and the artificial inoculation method in vivo. The clinical therapy effects were evaluated by mortality rate, liver function-related biochemical indicators, and visual changes in pathological anatomy. The anti-DHAV proliferation effects of APS, pAPS, and sAPS on the viral multiplication process in cell and blood were observed with the reverse transcription-polymerase chain reaction method. The results revealed that pAPS inhibited DHAV proliferation more efficiently in the entire process of viral multiplication than APS and sAPS. Moreover, only pAPS significantly improved the survival rate to 33.5% and reduced the DHAV particle titer in the blood as well as liver lesions in clinical trials. The results indicated that pAPS exhibited greater anti-DHAV activity than APS and sAPS both in vitro and in vivo.
研究领域:
病毒学
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药理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
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