APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2020
·Vol. 104
·期 18
·7787-7799
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IF 3.53
Yu Shengzhu,Guo Liwei,Zhao Luyao,Chen Zhenya,Huo Yixin
Phloroglucinol is a three-hydroxyl phenolic compound and has diverse physiological and pharmacological activities such as antivirus and anti-inflammatory activities. Chemical synthesis of phloroglucinol suffered from many drawbacks such as high cost and environmental pollution. To avoid the above issues, microbial phloroglucinol biosynthesis was successfully accomplished in this study, while the abundant and low-cost acetate was used as the main carbon source. Firstly, the toxicity of phloroglucinol was tested, and E. coli BL21(DE3) could tolerate 5 g/L phloroglucinol. The ability of phloroglucinol synthase (PhlD) for catalyzing malonyl-CoA to phloroglucinol was confirmed, and E. coli BL21(DE3) expressing PhlD and acetyl-CoA carboxylase (ACCase) could produce 1107 ± 12 mg/L phloroglucinol from glucose. Then, E. coli BL21(DE3) was engineered to utilize acetate to produce 228 ± 15 mg/L phloroglucinol. Then, the endogenous citrate synthase (GltA) which could catalyze oxaloacetate and acetyl-CoA generated from acetate to citrate was knocked down by CRISPRi system in order to enhance the carbon flux for phloroglucinol production, and the titer was improved to 284 ± 8 mg/L. This work demonstrated that acetate could be used as low-cost substrate to achieve the biosynthesis of phloroglucinol and provided an example of effective utilization of acetate.
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Frontiers in Pharmacology
·
2023
·
IF 5.6
Jing Chang,Jiahui Wang,Beibei Luo,Weihao Li,Ziyue Xiong,Chaoqi Du,Xue Wang,Yuejiao Wang,Jingya Tian,Shuxin Li,Yue Fang,Longjie Li,Jing Dong,Ke Tan,Yumei Fan,Pengxiu Cao
Introduction: Pulmonary fibrosis (PF) is a fatal chronic lung disease that causes structural damage and decreased lung function and has a poor prognosis. Currently, there is no medicine that can truly cure PF. Vitamin E (VE) is a group of natural antioxidants with anticancer and antimutagenic properties. There have been a few reports about the attenuation of PF by VE in experimental animals, but the molecular mechanisms are not fully understood. Methods: Bleomycin-induced PF (BLM-PF) mouse model, and cultured mouse primary lung fibroblasts and MLE 12 cells were utilized. Pathological examination of lung sections, immunoblotting, immunofluorescent staining, and real-time PCR were conducted in this study. Results: We confirmed that VE significantly delayed the progression of BLM-PF and increased the survival rates of experimental mice with PF. VE suppressed the pathological activation and fibrotic differentiation of lung fibroblasts and epithelial-mesenchymal transition and alleviated the inflammatory response in BLM-induced fibrotic lungs and pulmonary epithelial cells in vitro . Importantly, VE reduced BLM-induced ferritin expression in fibrotic lungs, whereas VE did not exhibit iron chelation properties in fibroblasts or epithelial cells in vitro . Furthermore, VE protected against mitochondrial dysmorphology and normalized mitochondrial protein expression in BLM-PF lungs. Consistently, VE suppressed apoptosis in BLM-PF lungs and pulmonary epithelial cells in vitro . Discussion: Collectively, VE markedly inhibited BLM-induced PF through a complex mechanism, including improving iron metabolism and mitochondrial structure and function, mitigating inflammation, and decreasing the fibrotic functions of fibroblasts and epithelial cells. Therefore, VE presents a highly potential therapeutic against PF due to its multiple protective effects with few side effects.
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LANGMUIR
·
2023
·
IF 3.9
Qianli Sheng,Min Yuan,Dan Wang,Yuanqi Kou,Lei Liu,Yan Chen,Shiyong Song
Vitamin E derivatives are particularly effective in chemotherapy drug development because they are nontoxic, biocompatible, and selective. Among them, α-tocopheryl succinate (α-TOS) can act synergistically with some chemotherapeutic agents. However, its hydrophobicity limits its systemic administration, and localized formulations are not available. Herein, we developed an injectable hydrogel based on self-assembled micelles of a triblock amphiphilic derivative of α-TOS (PEG-2VES), in which doxorubicin (DOX) was encapsulated in the core of the micelles for combined chemotherapy. A molecule of α-TOS was grafted onto each end of poly(ethylene glycols) (PEGs) of different lengths. Hydrogels were prepared by dissolving the polymers or the DOX-loaded micelles in water at room temperature. The subcutaneously injected hydrogels kept their shape and sustainably released the payloads over 7 days without any noticeable inflammatory response. In vitro and in vivo results confirmed the synergistic antitumor effects of the hydrogel and loaded drug. Furthermore, DOX-loaded hydrogels showed greater therapeutic efficiency and fewer toxic side effects than DOX alone. Overall, this hydrogel acts as a multifunctional system that can deliver drug, improve the therapeutic effect, and minimize drug toxicity.
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ULTRASONICS SONOCHEMISTRY
·
2019
·
IF 7.279
Lin Lin,Xinlei Wang,Changzhu Li,Haiying Cui
Ultrasonic sterilization (US), as a promising non-thermal sterilization method, exhibits unique superiorities than traditional sterilization methods. In this study, the inactivation mechanism of E. coli O157:H7 under US was investigated in cucumber and bitter gourd vegetable juices. Results revealed that the US treatment showed good antibacterial ability in countering E. coli O157:H7. Through determinations of conductivity and β -galactosidase activity, significant augmentation in membrane permeability of the bacteria was confirmed after the US treatment. The morphologies of the US treated E. coli O157:H7 demonstrated that the integrity of the cell membrane was disrupted by US treatment. SDS-PAGE and LSCM data further proved the disruptive action of US, leading to the leakage of proteins and DNA through the breakage on cell membrane. The decrease of metabolic-related enzyme activity was verified through investigation of bacterial metabolism. The antibacterial mechanism analysis indicated that the US can generate free radicals which resulted in the rise of intracellular oxidative stress, attenuation of energy metabolism and inhibition of hexose monophosphate pathway. As the application verification, the US treatment can cause the deprivation of E. coli O157:H7 cell viability in vegetable juices without obvious impact on the sensory quality.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Huacui Xiang,Zhijian Li,Zhou Bai,Haiwei Wu,Guodong Liu,Hongwei Zhou,Hanbin Liu
Wearable human–machine interaction remains a huge challenge when various flexible electronics have been developed to date, for which the information input seems more difficult than the information output such as display devices. Here in this work, an e-skin for handwriting input was disclosed based on a double network hydrogel of gluten protein and polyvinyl alcohol (PVA) that is cross-linked by borax. The hydrogel can tightly adhere onto human skin, being stretchable, self-healable, and working properly even under breakage, which perfectly match the requirements of the e-skin for human–machine interaction. Under the assistant of deep learning, the handwriting of letter and words on it can be recognized with high accuracy of above 89%, even under tensile state or destruction. In addition, this flexible device is biocompatible and biodegradable benefiting from the protein and PVA networks, which make it suitable as a wearable e-skin and being free of electronic wastes. The finding of this work may open a window for the development of e-skin for the two-way human–machine interaction.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Xingchun Zhai,Junjie Li,Weiwei Cheng,Xiaolu Li,Yongheng Zhang,Bingxue Cao,Junjie Wen,Ninghui Zhu,Da Wu,Tao Wang,Fuzhen Xuan,Guoyue Shi,Min Zhang
This study introduces a radial-hierarchical, diffusion-enhanced spatiotemporal sensing paradigm for volatile organic compound (VOC) analysis via an integrated microchamber paper-based chromatomimetic e-nose. The proposed system synergizes interlayer spatiotemporal dynamics with planar spatial variance by employing a radially symmetric electrode array and a hierarchical porous chemoresistive ink (CuP@G). This design leverages molecular diffusion gradients across the sensing plane, enabling precise discrimination of complex VOC mixtures through multidimensional “spatiotemporal fingerprints”. A physics-informed framework integrates molecular transport principles with multitask learning convolutional neural network (MTL-CNN) analytics, achieving unprecedented resolution in real-sample classification. Systematic validation demonstrates superior performance in discriminating diverse VOCs, binary mixtures, and authentic tobacco samples (origin and level classification accuracy: 92–99%). This work establishes a scalable blueprint for high-fidelity VOC analytics, bridging gas diffusion physics with intelligent signal processing to advance e-nose technology toward precision-driven design.
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Biology-Basel
·
2025
·Vol. 14
·期 8
·992
·
IF 3.5
Kaicheng Gu,Lindong Yang,Yi Jiang,Zhiqiang Wang,Jiannan Chen
Simple SummaryBisphenol E (BPE), a typical endocrine disruptor, is widely present in various environmental media. Using zebrafish as a model, this study systematically evaluated the toxic effects of BPE on embryonic development and the nervous system. By integrating a network toxicology analysis and molecular docking techniques, the neurotoxic mechanism was revealed: BPE inhibits the activity of NOS3/PKG, blocks the transmission of the cGMP/PKG signaling pathway, and ultimately induces neurotoxic damage. This research advances the understanding of endocrine disruptor toxicity by providing novel experimental evidence and delivers key theoretical pillars for building a health risk assessment system targeting environmental pollutants.As typical environmental hormones, endocrine-disrupting chemicals (EDCs) have become a global environmental health issue of high concern due to their property of interfering with the endocrine systems of organisms. As a commonly used substitute for bisphenol A (BPA), bisphenol E (BPE) has been frequently detected in environmental matrices such as soil and water in recent years. Existing research has unveiled the developmental and reproductive toxicity of BPE; however, only one in vitro cellular experiment has preliminarily indicated potential neurotoxic risks, with its underlying mechanisms remaining largely unelucidated in the current literature. Potential toxic mechanisms and action targets of BPE were predicted using the zebrafish model via network toxicology and molecular docking, with RT-qPCRs being simultaneously applied to uncover neurotoxic effects and associated mechanisms of BPE. A significant decrease (p< 0.05) in the frequency of embryonic spontaneous movements was observed in zebrafish at exposure concentrations ≥ 0.01 mg/L. At 72 hpf and 144 hpf, the larval body length began to shorten significantly from 0.1 mg/L to 1 mg/L, respectively (p< 0.01), accompanied by a reduced neuronal fluorescence intensity and a shortened neural axon length (p< 0.01). By 144 hpf, the motor behavior in zebrafish larvae was inhibited. Through network toxicology and molecular docking, HSP90AB1 was identified as the core target, with the cGMP/PKG signaling pathway determined to be the primary route through which BPE induces neurotoxicity in zebrafish larvae. BPE induces neuronal apoptosis and disrupts neurodevelopment by inhibiting the cGMP/PKG signaling pathway, ultimately suppressing the larval motor behavior. To further validate the experimental outcomes, we measured the expression levels of genes associated with neurodevelopment (elavl3,mbp,gap43,syn2a), serotonergic synaptic signaling (5-ht1ar,5-ht2ar), the cGMP/PKG pathway (nos3), and apoptosis (caspase-3,caspase-9). These results offer crucial theoretical underpinnings for evaluating the ecological risks of BPE and developing environmental management plans, as well as crucial evidence for a thorough comprehension of the toxic effects and mechanisms of BPE on neurodevelopment in zebrafish larvae.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Cong Chen,Fei Yu,Xinli Wen,Shuna Chen,Kaixi Wang,Feiquan Wang,Jianming Zhang,Yuanyuan Wu,Puming He,Youying Tu,Bo Li
Two major green leaf volatiles (GLVs) in tea that contribute greatly to tea aroma, particularly the green odor, are (E)-2-hexenal and (Z)-3-hexenal. Until now, their formation and related mechanisms during tea manufacture have remained unclear. Our data showed that the contents of (E)-2-hexenal and (Z)-3-hexenal increased more than 1000-fold after live tea leaves were torn. Subsequently, a new (Z)-3:(E)-2-hexenal isomerase (CsHI) was identified in Camellia sinensis . CsHI irreversibly catalyzed the conversion of (Z)-3-hexenal to (E)-2-hexenal . Abiotic stresses including low temperature, dehydration, and mechanical wounding, did not influence the (E)-2-hexenal content in intact tea leaves during withering, but regulated the proportions of (Z)-3-hexenal and (E)-2-hexenal in torn leaves by modulating CsHI at the transcript level. For the first time, this work reveals the formation of (E)-2-hexenal during tea processing and suggests that CsHI may play a pivotal role in tea flavor development as well as in plant defense against abiotic stresses.
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DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
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2025
·
IF 1.8
Taobo Liang,Junyu Cao,Liyan Zhuo,Chunhong Zhu,Yufei Wang,Shanshan Hu,Jiaxing Hao,Guangsheng Zhuo,Tao Wang
Microbial contamination during the collection of placental and umbilical cord tissues presents a significant challenge to the safety of stem cell-based therapies and the commercial storage. In this study, microbial contamination analysis of a large cohort placental and umbilical cord samples collected recent revealed with 38 distinct microbial species identified. Contaminated samples were predominantly from vaginal deliveries (82.7%), and higher contamination rates were observed in mothers aged ≥30 years. For rapid monitoring, we developed a novel multiplex quantitative PCR assay for simultaneous detection of the most prevalent contaminants: Escherichia coli, Candida albicans , and Enterococcus faecalis . The assay demonstrated robust detection limits in pure cultures, with a lower limit of detection of 10 2 CFU/mL for E. coli and C. albicans , and 10 1 CFU/mL for E. faecalis . Based on the analytical performance described above, this multiplex qPCR assay establishes a robust framework for rapid, quantitative surveillance of microbial contaminants in placental-derived biologics. By enabling same-shift screening with sensitivity down to 10 CFU/mL, the method significantly enhances quality assurance protocols for stem cell banking a critical advancement toward safer clinical translation of placental-based therapeutics.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Hongyue Li,Yang Wang,Jiaxin Zhang,Xuepeng Li,Jinxiang Wang,Shumin Yi,Wenhui Zhu,Yongxia Xu,Jianrong Li
Electronic nose (E-nose), electronic tongue (E-tongue) and colorimeter combined with data fusion strategy and different machine learning algorithms (artificial neural network, ANN; extreme gradient boosting, XGBoost; random forest regression, RFR; support vector regression, SVR) were applied to quantitatively assess and predict the freshness of horse mackerel ( Trachurus japonicus ) during the 90-day frozen storage. The results showed that the fusion data of the E-nose, E-tongue and colorimeter could contain more information (with a total variance contribution rate of 94.734 %) than that of the independent one. ANN, RFR and XGBoost showed good performance in predicting biochemical indexes with the R P 2 (the square correlation coefficient of the Test set) ≥ 0.929, 0.936, 0.888, respectively, while SVR models showed a bad performance ( R P 2 ≤ 0.835). In addition, among the established quantitative models, the RFR model had the best prediction effect on K value (freshness index) with R p 2 of 0.936, ANN model had the highest fitting degree in predicting carbonyl content (protein oxidation degree) with R p 2 of 0.978, XGBoost model had the best performance in predicting the TBA value (lipid oxidation degree) with R p 2 of 0.994, RFR model was the best strategy for predicting Ca 2+ -ATPase activity (protein denaturation degree) with R p 2 of 0.969. The results demonstrated that the freshness of frozen fish can be effectively evaluated and predicted by the combination of electronic sensor fusion signals.
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Food Bioscience
·
2025
·
IF 5.9
Yuwei Liu,Yuting Wang,Ziyi Liu,Chang Li,Qiang Yu,Jianhua Xie,You Long,Qianzhu E,Yi Chen
Spice use in traditional braised He Bao carp (Cyprinus carpio) remains empirical, and its flavor-modulating mechanisms lacks scientific elucidation. This study elucidated the differential flavor-modulating mechanisms of four natural spices—Perilla frutescens, dried tangerine peel (Citrus reticulata), lemongrass (Cymbopogon citratus), and orange peel (Citrus sinensis). Aroma-active and taste-active compounds were characterized by GC–MS, GC–IMS, E-nose, E-tongue, and molecular docking. A total of 129 volatiles were identified, including 16 shared core compounds (e.g., benzaldehyde, nonanal, geraniol). Lemongrass group yielded the highest volatile content (110.34 mg/kg) with dominant citral, geraniol, citronellol, and eugenol, while orange peel group exhibited abundant D-limonene (9.93 mg/kg, OAV > 200). Perilla frutescens group suppressed fishy odor by reducing 1-octen-3-ol, and dried tangerine peel group enriched thymol and γ-terpinene, enhancing herbal-citrus notes. Molecular docking revealed stronger binding of key pleasant odorants (–4.4 to –5.1 kcal/mol) to OR1D2 receptors than off-flavor compounds. In taste evaluation, dried tangerine peel group and lemongrass group enhanced umami perception via increased Glu (≈1.09 g/100 g) and 5′-IMP (up to 258.7 mg/100 g). Overall, natural spices distinctly modulated aroma and taste, with lemongrass group and orange peel group producing complex aromatic profiles and dried tangerine peel group improving taste balance. These findings provide mechanistic insight and practical guidance for enhancing the flavor and standardization of traditional Jiangxi cuisine.
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TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2026
·
IF 3.6
Shangxiang Feng,Kun Song,Hao Zhang,Zhuoqun Wang,Yuchi Zhao,Zhongyuan Zhao,Chao Wan
This study investigated the effects of Aureusidin on extracellular matrix (ECM) degradation and pyroptosis of chondrocytes in osteoarthritis. Network pharmacology, molecular docking, and pull-down assays were initially conducted to explore the target of Aureusidin. Subsequently, Sprague-Dawley rats underwent anterior cruciate ligament transection (ACLT) received oral administration of Aureusidin. Cartilage damage was evaluated histopathologically by H&E and Safranin O-fast green staining. Primary rat chondrocytes were treated with Aureusidin under IL-1β stimulation. Pyroptotic morphology was observed under light microscopy. Propidium iodide staining and flow cytometry were conducted to evaluate membrane rupture. The expression of markers associated with ECM degradation and pyroptosis was analyzed by qRT-PCR, Western blot, immunohistochemistry, and ELISA. Caspase-3 was identified as a target for Aureusidin. Aureusidin (5, 10, 20 mg/kg) alleviated ACLT-induced cartilage damage and ECM degradation. It reduced the OARSI score and serum levels of CTX-II and COMP, while upregulating the expression of Col2a1 and Acan , and downregulating Adamts5 , Mmp13 , and Mmp3 . Aureusidin also mitigated atypical pyroptosis, evidenced by inhibited expression of GSDME-N, Caspase-3, and Cleaved-Caspase-3, and decreased levels of IL-1β and IL-18. In IL-1β-stimulated primary chondrocytes, Aureusidin (5, 10, 20 μM) attenuated ECM degradation and Caspase-3/GSDME-mediated pyroptosis. Treating chondrocytes with the Caspase-3 inhibitor Z -DEVD-FMK under IL-1β-stimulated conditions alleviated ECM degradation and pyroptosis, but the additional application of Aureusidin did not provide further inhibition. Aureusidin has the potential to inhibit ECM degradation and Caspase-3/GSDME-mediated chondrocyte pyroptosis during osteoarthritis progression. Its therapeutic effects are dependent on binding to and modulating the Caspase-3.
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