ACS Applied Nano Materials
·
2019
·
IF 0
Yanan Sun,Yuwei Wu,Chaoran Yu,Li Zhang,Gang Song,Zhiyi Yao
We report nanoscale pyrenyl excimers induced by the self-assembly of a pyrene-based fluorescent probe, 12-pyren-1-yldodecanoic acid (PyDA), for rapid detection of 2,4,6-trinitrotoluene (TNT) in aqueous media. Under the optimized conditions, the sensing nanosystem exhibited fast response and high selectivity and sensitivity for the detection of TNT. Especially, it could distinguish TNT from TNP, which is the major interferent in most circumstances. The detection limit is as low as 5.0 × 10–9 M. It also can be used for qualitatively and quantitatively monitoring TNT in real samples.
研究领域:
纳米技术
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
RSC Advances
·
2015
·Vol. 5
·期 30
·23981-23989
·
IF 3.84
Wei Xu,Chao Lin,Huan Liu,Hongbo Yu,Kai Tao,Shenghu Zhou
Tungsten substituted mesoporous FDU-12 (W-FDU-12) catalysts were synthesized by a one-pot hydrothermal process using F127 as the structure directing agent. The studies of TEM, SAXS and BET illustrated that the highly ordered mesoporous structure of FDU-12 was maintained in the doped W-FDU-12 samples. XPS studies revealed that a high concentration of W5+ species appeared in doped W-FDU-12 catalysts whereas supported WO3/FDU-12 and WO3/SiO2 catalysts only contained W6+ species. Tandem catalytic conversion of 1-butene and ethene to propene through isomerization of 1-butene to 2-butene and consecutive cross metathesis of 2-butene and ethene in a fixed-bed reactor at different temperatures and atmospheric pressure was used to evaluate the catalytic performance of the W-FDU-12 catalyst, combined with MgO. The catalytic results showed that the doped W-FDU-12 illustrated a superior catalytic performance relative to the supported WO3/FDU-12 and WO3/SiO2 catalysts. The higher metathesis activity of W-FDU-12 catalysts can be ascribed to the good dispersion of W species and the incorporation of W species into the framework of FDU-12, forming a substantial amount of W5+, which was beneficial for the cross metathesis of 2-butene and ethene to propene.
研究领域:
催化化学
·
化工工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2024
·
IF 15.8
Bin Wang,Maoping Tang,Qijing Chen,William Ho,Yilong Teng,Xiaojian Xiong,Zhitong Jia,Xiuling Li,Xiaoyang Xu,Xue-Qing Zhang
T cell exhaustion has emerged as a major hurdle that impedes the clinical translation of stimulator of interferon genes (STING) agonists. It is crucial to explore innovative strategies to rejuvenate exhausted T cells and potentiate the antitumor efficacy. Here, we propose an approach utilizing MSA-2 as a STING agonist, along with nanoparticle-mediated delivery of mRNA encoding interleukin-12 (IL-12) to restore the function of T cells. We developed a lipid nanoparticle (DMT7-IL12 LNP) that encapsulated IL12 mRNA. Our findings convincingly demonstrated that the combination of MSA-2 and DMT7-IL12 LNP can effectively reverse the exhausted T cell phenotype, as evidenced by the enhanced secretion of cytokines, such as tumor necrosis factor alpha, interferon gamma, and Granzyme B, coupled with reduced levels of inhibitory molecules such as T cell immunoglobulin and mucin domain-3 and programmed cell death protein-1 on CD8+ T cells. Furthermore, this approach led to improved survival and tumor regression without causing any systemic toxicity in melanoma and lung metastasis models. These findings suggest that mRNA encoding IL-12 in conjunction with STING agonists has the potential to confer superior clinical outcomes, representing a promising advancement in cancer immunotherapy.
研究领域:
癌症研究
·
免疫学
·
分子生物学
·
治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiawen Li,Qinbo Jiang,Meiyu Chen,Teng Li,Quancai Sun,Ye Peng,Yang Li,Yucheng Zou,Hui Zhang
This study aimed at improving the physical stability of the electrospinning fiber aerogel (EFA) templates and the templated oleogels (EFA-O), and study the effect of hydrophobic treatment on the properties of the templates and oleogels. The protein thermal denaturation and 12-hydroxystearic acid (12-HSA) loading were utilized to hydrophobically modify the electrospun fiber aerogel templates. Both of the two treatments increased the average fiber diameter, and 12-HSA loading made the EFA obtained a stable and lasting hydrophobicity (water contact angle was around 130°). Meanwhile, both hydrophobic treatments improved the stress feedback of EFA, and thermal denaturation could maintain the excellent resilient performance. The oil adsorption and holding capacity of EFA reached 48.77 g/g and 85.73 %, respectively, and the two low-degree hydrophobic methods were combined to prepare compositely treated EFA-O with excellent performances. The hydrophobic EFA framework supported the 3D structure of oleogels, where the fiber diameter could reach 464.01 nm. Thermal denaturation was beneficial to improve the rheological properties, and 12-HSA loading significantly strengthened the texture properties. Hydrophobic treatments could also effectively inhibit the release of free fatty acids (down to 23.84 %) or curcumin (down to 14.19 %) in EFA-O, which was promising for constructing stable controlled-release systems. The combination of the two treatments can achieve complementary modification effects and lead to better physical and chemical properties. This modification method was not only an effective way to strengthen the performances of EFA, but it met the edibility and safety requirements of oleogel preparation.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 26
·11336-11341
·
IF 3.288
Dongying Shi,Chao-Jie Cui,Chun-Xiao Sun,Jun-Ping Du,Chun-Sen Liu
A new [Co21(H2O)4(OH)12]30+ unit-incorporating polyoxotungstate, Na18[Co(H2O)6]2{[Na(H2O)4]2[Na(H2O)2]2[Co(H2O)3]2[Co21(H2O)4(OH)12](SiW10O37)6}·24H2O (denoted as Co25Si6W60), has been hydrothermally synthesized and fully characterized via elemental analyses, thermogravimetric analysis, bond valence sum (BVS) calculations, IR spectrum, UV-vis spectrum, diffuse reflectance spectrum, powder X-ray diffraction and single-crystal structural analysis. The most remarkable feature is that the gigantic [Co21(H2O)4(OH)12(SiW10O37)6]30− core in Co25Si6W60 is composed of a square-like [Co21(H2O)4(OH)12]30+ nanosheet encapsulated by six dilacunary Keggin [α-SiW10O37]10− moieties in a staggered fashion, resulting in a remarkable sandwich-type assembly. Furthermore, the unique Co25Si6W60-based biosensing system, as a simple, rapid, and highly sensitive electrochemical platform, offers an environmentally friendly route for detecting dichlorvos in the field of both food safety and environmental pollution.
研究领域:
无机化学
·
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2022
·
IF 5.546
Rongtao Liu,Zebiao Xu,Chen Zhao,Shiyang Zhang,Hongyu Zhou,Li Zhou,Yidong Liu,Yonggang Min
Although electrical stimulation (ES) has been proved to be an effective strategy in promoting the regeneration and repair of injured peripheral nerve tissue, it is still a big challenge to encounter the risk of inflammation and infection when the conductive biological platform directly contacted with the damaged tissue. Therefore, it is a very promising solution to develop non-invasive flexible electrodes’ bio-scaffolds for clinical treatment of nerve tissue regeneration combined with ES. With such purpose, the flexible composite electrodes by depositing polypyrrole on laser-induced graphene (LIG) have been developed. The proliferation and differentiation of PC-12 cells incubated on the prepared composite electrodes combined with an ES of 400 mV/cm at different times have also been investigated. The results showed that the fabricated composite electrodes exhibited excellent conductivity and wettability. Moreover, the essence of ES was to induce a field effect, namely, the cell proliferation was independent of the conductivity of the composite electrodes. In addition, we observed that ES could significantly enhance proliferation and differentiation of PC-12 cells. With the prolongation of ES time, the increasing trend finally stabilized. Importantly, the cell growth through MTT value was 1.37 when ES time was 4 h, and the increment was the largest, reaching 0.3, compared with that of 2 h. Furthermore, the cell differentiation showed that the neurite outgrowth length at 8 h of ES was the broadest distribution (the half-width ca 33.83 μm). And the average neurite length reached 68.39 μm after 8 h of ES, which increased by 16.26 μm compared with that of 4 h. This work inspires the design and construction of a practical conductive platform, and provided a new theoretical basis and data support for clinical therapy in nerve tissue engineering combined with ES.
研究领域:
生物医学工程
·
神经科学
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Yijing Zhang,Xisen Cao,Guang Hu,Rui Ye,Li Zhang,Jie Song
Interleukin-12 (IL-12) is a critical cytokine with notable anticancer properties, including enhancing T-cell-mediated cancer cell killing, and curbing tumor angiogenesis. To date, many approaches are evaluated to achieve in situ overexpression of IL-12, minimizing leakage and the ensuing toxicity. Here, it is focused on circular single-stranded DNA (Css DNA), a type of DNA characterized by its unique structure, which could be expressed in mammals. It is discovered that Css DNA can induce sustained luciferase expression for half a year by intramuscular injection and showed effective antitumor results by intratumoral injection. Motivated by these findings, a folate-modified LNP system is now developed for the delivery of Css DNA expressing IL-12 for the therapy of 4T1 triple-negative breast cancer (TNBC). This delivery system effectively activates anti-cancer immune responses, slows tumor growth, significantly prolongs survival in animal models, and prevents tumor recurrence. After 6 months of long-term observation, the elevated level of IL-12 is still detectable in the lymph nodes and serum of the cured mice. This study highlights the long-term sustained expression capacity of Css DNA and its ability to inhibit recurrence, and the potential of tumor-targeted LNPs for Css DNA-based cancer therapy, providing a new insight into gene overexpression strategy.
研究领域:
癌症生物学
·
分子生物学
·
基因治疗
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sensors & Diagnostics
·
2023
·Vol. 2
·期 2
·427-437
·
IF 0
Xiangfei Zheng,Dongmiao Qin,Shenglan Hu,Weiming Mo,Biyang Deng
Tin oxide nanoflowers (SnO2NFs) are used as both an electrochemiluminescent (ECL) signal generator and a carrier of nanomaterials due to the advantages of excellent electrical conductivity, large specific surface area, and good optical properties. AuNPs/PEI-SnO2NFs provide a larger binding site for the immobilization of secondary antibodies (CA12-5-Ab2). The synthesized Ag@ZnONS composite with a large specific surface area and good biocompatibility was applied to immobilize more primary antibodies (CA12-5-Ab1), which further enhanced the ECL signal and obviously improved the sensitivity and selectivity of the immunosensor. A novel signal amplification ECL immunosensor for detecting carbohydrate antigen 12-5 (CA12-5) was fabricated with AuNPs/PEI-SnO2NFs as a signal probe and Ag@ZnONSs as the sensing matrix. The ECL intensity was linearly related to the logarithm of the CA12-5 concentration in the range from 1 × 10−5 to 50 ng mL−1 under optimized conditions. The limit of detection was 2.6 fg mL−1 (S/N = 3). The assay had good operational stability, specificity, and high sensitivity. The study was applied to analyze the CA12-5 level in human serum with satisfactory results, which could be applied in clinical immunoassays.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
临床诊断
✓ 已验证
DOI
Virtual and Physical Prototyping
·
2023
·
IF 10.6
Yanbei Hou,Ming Gao,Jiayao Chen,Wei Shian Tey,Mei Chen,Han Zheng,Boyuan Li,Lihua Zhao,Kun Zhou
Multi jet fusion (MJF), a powder-based additive manufacturing technology, is suitable for fabricating fibre-reinforced polymer composites. However, the types of reinforcement fibres applied in MJF are limited due to the incompatibility between the fibres and polymer. Herein, a simple and cost-effective surface modification method for Fe3O4-coated aramid fibres (Fe3O4@AF hybrids) is proposed to fabricate polyamide 12 (PA12) composites by MJF. The optimal fibre content for Fe3O4@AF/PA12 composites is 6 wt%, which resulted in 10.83% and 16.05% higher ultimate tensile strength and Young’s modulus, respectively, compared with AF/PA12. The addition of Fe3O4@AF hybrids also improved the flame retardancy of AF/PA12 by reducing the peak heat release rate (PHRR) and postponing the temperature at PHRR. This practical surface modification method can be potentially applied to other reinforcement fibres for fabricating functional polymer composites by other powder-based manufacturing technologies.
研究领域:
材料科学
·
制造工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Wenbin Li,Tongmeng Xing,Peng Zhang,Bin Chen,Zhuo Zhang,Baiyang Zhou,Ting Ding,Jing Li,Lunyong Zhang
The photo-assisted enhancement, as well as the multifunctional energy-environment synergy not only demonstrate substantial research significance in electrocatalytic materials but also pose considerable scientific challenges. In this study, we propose a quadruple-functional photo-assisted electrocatalytic (PEC) system for efficient and monitorable polychlorinated biphenyl (PCB-12) dechlorination during alkaline water splitting. The system comprises Ni(OH) 2 S x nanosheets decorated with NiS/Ni 3 S 2 clusters and Ag 2 S large nanoparticles (NPs) embedded with small Ag NPs. This integrated material demonstrates four distinct and efficient functions: 1) water splitting with current density of 1 A/cm 2 at 1.62 V vs RHE; 2) PEC dechlorination and degradation of 10 −4 M PCB-12 within 50 h; 3) facilitation of Cl - capture and hot electron injection from Ag 2 S/Ag hetero-NPs; 4) monitoring of PCB degradation as in-situ surface-enhanced Raman spectroscopy (SERS) substrate. The results indicate that NiS/Ni 3 S 2 is conducive to the retention of H*/OH*, while the H*/OH* on the Ni(OH) 2 S x is prone to desorption, makes the two active sites beneficial for PCB dechlorination and water splitting, respectively. This study provides a novel strategy for the collaborative application of multifunctional photo-electrocatalysts in energy conversion and environmental remediation.
研究领域:
电催化
·
环境修复
·
能量转换
·
纳米材料
·
光谱学
✓ 已验证
产品关联
DOI
高影响因子
MEDIATORS OF INFLAMMATION
·
2016
·
IF 3.418
Xin Liu,Ning Wang,Yuanfeng Zhu,Yongjun Yang,Xiaoli Chen,Shijun Fan,Qian Chen,Hong Zhou,Jiang Zheng
Activated macrophages are the primary sources of IL-12, a key cytokine bridging innate and adaptive immunity. However, macrophages produce low amounts of IL-12 upon stimulation and the underlying regulatory mechanism remains unclear. In this study, we found a new calcium-dependent mechanism that controlled IL-12 production in LPS-treated murine macrophages. First, LPS was demonstrated to induce extracellular calcium entry in murine peritoneal macrophages and inhibition of calcium influx resulted in marked enhancement in IL-12 production. Then, withdrawal of extracellular calcium was found to suppress CaMKK and AMPK activation triggered by LPS while chemical inhibition or genetic knockdown of these two kinases augmented LPS induced IL-12 production. AMPK activation increased the NAD+/NADH ratio and activated Sirtuin 1 (SIRT1), a NAD+-dependent deacetylating enzyme and negative regulator of inflammation. Chemical inhibitor or siRNA of SIRT1 enhanced IL-12 release while its agonist suppressed IL-12 production. Finally, it was found that SIRT1 selectively affected the transcriptional activity of NF-κB which thereby inhibited IL-12 production. Overall, our study demonstrates a new role of transmembrane calcium mobilization in immunity modulation such that inhibition of calcium influx leads to impaired activation of CaMKKβ-AMPK-SIRT1 signaling pathway which lifts restriction on NF-κB activation and results in enhanced IL-12 production.
研究领域:
免疫学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nutrients
·
2025
·Vol. 17
·期 19
·3099
·
IF 5
Zhuo Liu,Wenxiu Wang,Ning Li,Jinkuan Chen,Qianxu Wang,Mengzhen Jia,Xiaorui Wang,Bo Zhang,Nan Sheng,Zhigang Liu
Background/Objectives: Infant diarrhea is a major global cause of morbidity and mortality. WhileBifidobacteriumis linked to diarrhea, its preventive effects, underlying mechanisms, and potential synergistic benefits with prebiotics remain unclear. The objective of this study was to explore the efficacy of a synbiotic composed ofBifidobacterium animalissubsp.lactisBB-12 (BB-12) and 2′-fucosyllactose (2′-FL) in alleviating infant diarrhea.Methods: One-week-old C57BL/6J mice were used to construct a model of infant diarrhea via infection with enteropathogenicEscherichia coli(EPEC) O127. Mice were administered BB-12 (108CFU per mouse), 2′-FL (1 g/kg), or their combination (synbiotic) for three consecutive weeks.Results: Administration of the synbiotic not only markedly improved diarrhea, anxiety-like behavior, colon inflammation, and gut barrier function but also positively reshaped the microbial community. This was achieved through a significant rise in short-chain fatty acid (SCFA)-producing bacteria (e.g.,AkkermansiaandParaprevotella), a rise in fecal SCFAs, and a reduction in harmful bacteria such asEscherichia.Conclusions: The synbiotic effectively relieves EPEC-induced infant diarrhea by regulating gut microbiota composition and metabolic functions. These findings highlight its potential as a dietary intervention in infant diarrhea and provide new insights into infant health applications.
研究领域:
微生物学
·
儿科
·
营养学
·
免疫学
·
分子生物学
·
肠道生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）