Food Analytical Methods
·
2017
·Vol. 11
·期 4
·1155-1162
·
IF 2.038
Zheng Yang,Peng Xiaofeng,Wu Yiwei
A sensitive method has been established by using activated carbon as an adsorbent for extraction of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) from bean sprout, tomato, potato, cucumber, and wheat grain prior to field-amplified sample injection-micellar electrokinetic capillary chromatography (FASI-MEKC) detection. Parameters affecting FASI and solid-phase extraction (SPE) were optimized in detail. Under the optimal conditions, the sensitivity of SPE-FASI-MEKC for IAA and IBA was respectively improved by 32.5 and 29.7 times as compared with the direct MEKC. The detection limits were 4.1 for IAA and 5.3 ng mL −1 for IBA with 4.9–5.2% RSD ( n = 9, C = 0.03 μg mL −1 ). The new method has been successfully applied to detect IAA and IBA in bean sprout, tomato, potato, cucumber, and wheat grain with the recoveries of 81.7–110%.
研究领域:
植物学
·
分析化学
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DOI
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Chen Lexian,Lv Qianqian,Cai Jianghong,Liang Jiajie,Liang Ziyan,Lin Jiahui,Xiao Ying,Chen Ruiyao,Zhang Zhiling,Hong Yue,Ji Hong
Coumarin derivatives have diverse structures and show various significant biological activities. Aiming to develop more potent coumarin derivatives for cancer treatment, a series of coumarin acrolein hybrids were designed and synthesized by using molecular hybridization approach, and investigated for their antiproliferative activity against A549, KB, Hela and MCF-7 cancer cells as well as HUVEC and LO2 human normal cells. The results indicated that most of the synthesized compounds displayed remarkable inhibitory activity towards cancer cells but low cytotoxicity on normal cells. Among all the compounds, 5d and 6e were the most promising compounds against different cancer cell lines, especially for A549 and KB cells. The preliminary action mechanism studies suggested that compound 6e, the representative compound, was capable of dose-dependently suppressing migration, invasion and inducing significant apoptosis. Furthermore, the combined results of network pharmacology and validation experiments revealed that compound 6e induced mitochondria dependent apoptosis via the PI3K/AKT-mediated Bcl-2 signaling pathway. In summary, our study indicated compound 6e could inhibit cell proliferation, migration, invasion and promote cell apoptosis through inhibition of PI3K/AKT signaling pathway in human oral epidermoid carcinoma cells. These findings demonstrated the potential of 3-(coumarin-3-yl)-acrolein derivatives as novel anticancer chemotherapeutic candidates, providing ideas for further development of drugs for clinical use.
研究领域:
药理学
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肿瘤学
·
化学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Linlin Ding,Qingjie Li,Kaige Qi,Yonghui Chen,Na Hu,Sangyu Hu,Tianqi Fang,Shuang Guan,Jianfeng Wang,Jiazhang Qiu,Xuming Deng,Lei Xu
Background Salmonella pullorum ( S. pullorum ) is an enteric pathogen that impairs growth performance, leading to substantial economic losses. Evidence demonstrates that the natural flavonoid phloretin can modulate intestinal microbiota functionality, and which underpins its therapeutic efficacy in ameliorating gastrointestinal disorders. However, the protective effects of phloretin against S. pullorum infections and underlying mechanisms remain unelucidated. Purpose This study aimed to elucidate the protective effects and mechanisms of phloretin in improving defense against S. pullorum infection by modulating gut microbiota in chicks. Methods H&E staining, RT-qPCR and ELISA assays were used to assess the protective potentials of phloretin in S. pullorum -infected chicks. Then, 16S rRNA gene sequencing and untargeted metabolomics were employed to identify key microbiota and metabolites regulating the intestinal microenvironment. Moreover, fecal microbiota transplantation (FMT) and dietary metabolite supplementation were conducted to reshape the gut microbiota, elucidate the interaction between the microbiota and S. pullorum infection. Results Phloretin treatment alleviated intestinal injury and enhanced growth performance in S. pullorum -infected chicks via improved intestinal barrier integrity, suppression of inflammatory responses, and restructuring of gut microbial composition. Additionally, these beneficial effects were also observed following FMT from phloretin-treated donors. Subsequent microbial and untargeted metabolomic analysis revealed that phloretin significantly enriched abundance of the functional bacterium Faecalibacterium , and the microbiota-derived phenylalanine metabolites 3-phenylpropionic acid (3-PPA). Importantly, 3-PPA supplementation attenuates S. pullorum -induced intestinal barrier damaged and inflammation in chicks through modulation of the AhR/IL-22/STAT-3 signalling axis. Conclusion These findings provide new insights into the therapeutic potentials of phloretin for S. pullorum -infected chicks.
研究领域:
微生物学
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免疫学
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分子生物学
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兽医学
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代谢组学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2018
·
IF 4.019
Chen Xin,Tang Hongzhi,Liu Yongdi,Xu Ping,Xue Yong,Lin Kuangfei,Cui Changzheng
3-Hydroxybenzoate 6-hydroxylase, an NADH-dependent flavoprotein, can convert 3-hydroxybenzoate which is an important intermediate in the biodegradation of many aromatic hydrocarbons. 3-Hydroxybenzoate is metabolized by entering the TCA cycle through the gentisate pathway. We found a putative 3HB6H gene from a cluster that potentially encodes for gentisic acid degradation from a halophilic Martelella sp. strain AD-3. The corresponding protein was expressed with an N-terminal His-tag and purified by Ni2+-nitrilotriacetic acid affinity chromatography. The protein showed an overexpressed band of about 46 kDa by SDS–PAGE, and it was also proven that the enzyme contains FAD by absorption spectroscopy and HPLC analysis. The optimal activity of 3HB6H from strain AD-3 was observed in phosphate buffer (pH 8.0) at 37∘C without salinity (NaCl) and metal salts. The Km values of 3-hydroxybenzoate 6-hydroxylase were determined to be 72.6 ± 10.1 μM and 104.1 ± 18.2 μM for 400 μM NADH and 3-hydroxybenzoate, respectively. Site-directed mutagenesis showed that residues 305, 306 and 308 are important for FAD binding. In addition, we found that Tyr221 and Gln305 of 3HB6H from strain AD-3 are involved in substrate binding.
研究领域:
微生物学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2018
·
IF 2.441
Qiong Lou,Jun Wen,Yuanying Jiang,Jinghua Huang,Guorong Fan,Fenyun Song
A reliable liquid chromatography/tandem mass (LC-MS/MS) method was developed for quantitation of 3′, 4′-dimethoxy flavonol-3-β- d -glucopyranoside (DF3G) in rat plasma using pantoprazole as the internal standard. An Agilent C18 column (100 mm × 3 mm, 3.5 μm) was performed for chromatographic separation with the mobile phase composed of methanol-water (containing formic acid) at a flow rate of 0.4 mL/min. A triple-quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source in positive ion mode was applied to quantitative analysis by multiple reacting monitoring (MRM). The MRM precursor-product ion transition of DF3G and Pantoprazole (IS) were m / z 461.1 → 299.2 and 383.9 → 200.2, respectively. Calibration curves were recovered in a concentration range of 1–500 ng/mL for plasma with a limit of lower quantification (LLOQ) of 1 ng/mL. The intra-day and inter-day precision were not >10%. The accuracy of DF3G ranged from −10% to 0.53% in quality control (QC) samples at three concentrations. DF3G was not degraded during the analysis and the storage period. All the data were validated in accordance with the FDA bioanalytical method validation guideline. The LC-MS/MS method was successfully employed in the pharmacokinetic study of the DF3G after oral administration and intravenous injection in rats.
研究领域:
分析化学
·
药物代谢
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2022
·Vol. 30
·期 8
·3209-3217
·
IF 4.705
Han Xiaolong,Jin Yujuan,Huang Jian,Tian Huafeng,Guo Maolin
Hyperbranched polymer with flexible long chains (Ɛ-CL) were synthesized based on hydroxyl terminated hyperbranched polyesters (HBPEs). The prepared HBP-CLs were used to toughen poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by melt-blending. Comparing with pure PHBV, the crystallinity (X c ) of the PHBV/3.0phr HBP-CLs blends decreased by 10.2% (from 67.8 to 60.9%), the impact strength increased by 533.3% (from 5.1 kJ·m − 2 to 32.3 kJ·m − 2 ), and the elongation at break increased by 160.5% (from 1.62 to 4.22%). With the increase of HBP-CLs, the fracture surface of the PHBV/HBP-CLs blends became rough and a significant decrease in crystallization area can be seen from POM images. Even filamentous structures and tiny holes was formed, which further demonstrated that HBP-CLs acted as an excellent toughening effect on PHBV.
研究领域:
材料科学
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高分子科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 139
·期 4
·51551
·
IF 3.125
You Wu,Yujuan Jin,Jian Huang,Huafeng Tian,Yunxuan Weng
Phenyl terminated hyperbranched polyesters (HBP-Bs) were synthesized based on Boltorn-type hydroxyl terminated HBPEs and characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, gel permeation chromatography, and so forth. The prepared HBP-Bs were used to toughen poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) directly by melt blending. The thermal properties, rheological properties, mechanical properties, and microstructure of the PHBV/HBP-Bs blends were studied in detail. Comparing with pure PHBV, the crystallinity of the PHBV/0.5 phr HBP-Bs blends decreased by 14.10% (from 64.39% to 55.04%), the impact strength increased by 158.30% (from 7.41 kJ·m −2 to 19.14 kJ·m −2 ), and the elongation at break increased by 400.00% (from 0.91% to 4.55%). It can be seen from the scanning electron microscope that the fracture surface of the PHBV/HBP-Bs blends became rough with the increase of HBP-Bs, even with large filamentous structures and tiny holes, which further demonstrated that HBP-Bs acted as an excellent toughening effect on PHBV.
研究领域:
材料科学
·
高分子科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Shimeles Addisu Kitte,Fuad Abduro Bushira,Tesfaye Refera Soreta
Efficient coreactants are of high importance in electrochemiluminescence sensing. Herein, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide was demonstrated as an effective coreactant for tris(2,2′-bipyridine)ruthenium(II) electrochemiluminescence system for the first time. The new electrochemiluminescence system showed a good sensing performance for H 2 O 2 on the basis of quenching of the electrochemiluminescence intensity. It allows the detection of H 2 O 2 in the dynamic range of 0.5–200 µM. The limit of detections for H 2 O 2 was 0.33 µM ( 3σ/m ). In addition, the developed ECL system exhibited satisfactory recoveries for H 2 O 2 sensing in real samples.
研究领域:
分析化学
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
Polymer Chemistry
·
2016
·Vol. 7
·期 39
·6120-6132
·
IF 5.687
Cai Wang,Yudong Zheng,Yi Sun,Jinsheng Fan,Qiujing Qin,Zhenjiang Zhao
A novel block polyurethane was designed and synthesized based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), 4,4′-diphenylmethanediisocyanate (MDI) and poly(ethylene glycol) (PEG). The obtained polyurethanes were characterized by proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), differential scanning calorimetry (DSC) and wide angle X-ray diffraction (WAXD). Furthermore, the biodegradability of the synthesized polyurethanes was evaluated at 37 °C in phosphate buffer solution (PBS) at pH 7.4. The results showed that these PHBV-based polyurethanes were degradable in PBS, as indicated by weight loss measurements, scanning electron microscopy (SEM) and FTIR. The cytotoxicity of the novel polyurethane was evaluated using the cell counting kit-8 (CCK8) assay in vitro. Preliminary evaluation of hemocompatibility was carried out via the hemolysis test, dynamic blood coagulation time and platelet adhesion. The current work provides a promising approach to prepare nontoxic and biodegradable polyurethanes with excellent mechanical properties and good hemocompatibility. This new material may find potential application in blood vessel tissue engineering.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
高影响因子
Canadian Journal of Chemistry
·
2016
·Vol. 94
·期 5
·
IF 1.003
Yingying Wu,Xuemin Chen,Yanying Wang,Chunya Li
3-Decyl-1-(3-pyrrole-propyl)imidazolium tetrafluoroborate (DPIMBF4) ionic liquid was synthesized and characterized. DPIMBF4 ionic liquid not only possesses a pyrrole group that can be electrochemically polymerized onto a glassy carbon electrode surface by using a multipotential step technique, but it also contains a long carbon chain that can improve the stability of a polymerized ionic liquid film in an aqueous solution. X-ray photoelectron spectroscopy, scanning electron microscope, and electrochemical impedance spectroscopy were used to confirm the successful polymerization of the ionic liquid. Voltammetry was employed to investigate the electrochemical behaviors of an environmental estrogen, hexestrol, at the polymerized ionic liquid film electrode. Hexestrol presents an irreversible oxidation peak at the polymerized DPIMBF4 ionic liquid film electrode. Compared with the bare glassy carbon electrode, the oxidation peak of hexestrol increased significantly on the polymerized DPIMBF4 ionic liquid film electrode. The oxidation peak current was found to be linearly related to hexestrol concentration in the range of 5.0 × 10−9 to 1.0 × 10−5 mol L−1. The detection limit was calculated to be 1.25 × 10−9 mol L−1 (S/N = 3). Hexestrol in crucian meat was determined using the polymerized DPIMBF4 ionic liquid film electrode with good accuracy.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
INORGANIC CHEMISTRY
·
2020
·
IF 4.825
Xuehua Dong,Ying Long,Xiaoyu Zhao,Ling Huang,Hongmei Zeng,Zhien Lin,Xin Wang,Guohong Zou
Two new antimony fluoride sulfates named A6Sb4F12(SO4)3 (A = Rb, Cs) with unique crown-like clusters have been successfully synthesized by introducing Sb3+ with a stereochemically active lone pair in the sulfate system through a facile hydrothermal method. The structure regulation induced by the cation sizes results in the symmetry difference in these two title compounds, i.e., Rb6Sb4F12(SO4)3 crystallizing in the polar noncentrosymmetric (NCS) space group of P3 and Cs6Sb4F12(SO4)3 in the centrosymmetric (CS) space group of P1̅. Detailed characterizations, including XRD, thermal behaviors, optical properties, and the theoretical calculations, for these two compounds have been performed. The discovery of the unique crown-like clusters observed in A6Sb4F12(SO4)3 (A = Rb, Cs) enriches the diversity of structures for antimony sulfates systems. Simultaneously, a summary and comparison for all reported antimony halide sulfates has been made to illustrate the synergistic effect of the stoichiometric ratio and cation sizes on the macroscopic centricity and the framework structure, which will guide the systematical discovery of antimony(III)-based functional materials in future.
研究领域:
无机化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2013
·
IF 2.906
Xiaowei Zhang,Boyan Gao,Fang Qin,Haiming Shi,Yuangrong Jiang,Xuebing Xu,Liangli (Lucy) Yu
The present study was conducted to test the hypothesis that a free radical was formed and mediated the formation of 3-monochloropropanediol (3-MCPD) fatty acid diesters, a group of food contaminants, from diacylglycerols at high temperature under a low-moisture condition for the first time. The presence of free radicals in a vegetable oil kept at 120 °C for 20 min was demonstrated using an electron spin resonance (ESR) spectroscopy examination with 5,5-dimethylpyrroline-N-oxide (DMPO) as the spin trap agent. ESR investigation also showed an association between thermal treatment degree and the concentration of free radicals. A Fourier transform infrared spectroscopy (FT-IR) analysis of sn-1,2-stearoylglycerol (DSG) at 25 and 120 °C suggested the possible involvement of an ester carbonyl group in forming 3-MCPD diesters. On the basis of these results, a novel free radical mediated chemical mechanism was proposed for 3-MCPD diester formation. Furthermore, a quadrupole-time of flight (Q-TOF) MS/MS investigation was performed and detected the DMPO adducts with the cyclic acyloxonium free radical (CAFR) and its product MS ions, proving the presence of CAFR. Furthermore, the free radical mechanism was validated by the formation of 3-MCPD diesters through reacting DSG with a number of organic and inorganic chlorine sources including chlorine gas at 120 and 240 °C. The findings of this study might lead to the improvement of oil and food processing conditions to reduce the level of 3-MCPD diesters in foods and enhance food safety.
研究领域:
食品科学
·
化学
✓ 已验证
DOI
高影响因子
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