Horticulturae
·
2023
·Vol. 9
·期 7
·789
·
IF 3.1
Tao Xu,Min Zhang,Tianchi Chen,Lili Gong,Lingling Hu,Jie Yang,Haoxuan Si,Yueyan Wu
Suboptimal light stress in grapevines is increasing worldwide with the spread of grapevine cultivation, which can affect grapevine physiology and productivity, such as in Southern China. Abscisic acid (ABA) is an important hormone in plant adaptive responses to abiotic stress, including low light stress. However, ABA signaling pathway genes (APGs) are not well characterized in the grapevine, and little is known of their potentially mitigating role in grapevine growth under weak light stress. Our study aimed to explore the potential role of the ABA signaling pathway in the response of grapevines to suboptimal light conditions. In this study, APGs were identified in the grapevine genome, and the distribution of conserved motifs was shown to reflect their phylogenetic relationships. Gene duplication analysis indicated that segmental duplication was an important driver for gene expansion in the grapevine ABA signaling pathway. Suboptimal conditions of light were shown to seriously affect the growth of grapevine leaves and berries, with the differential regulation of APGs in the grapevine. Our study summarizes the basic characteristics of APGs in grapevine, which can now be examined further for their roles in grapevine’s response to suboptimal light conditions.Keywords:grape;weak light stress;abscisic acid;gene family
研究领域:
植物科学
·
分子生物学
·
胁迫生理学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2018
·
IF 3.677
Li Zhan,Gao Yue,Zhang Yuchan,Lin Cheng,Gong Dongting,Guan Yajing,Hu Jin
Seed germination is a complex process controlled by various mechanisms. To examine the potential contribution of reactive oxygen species (ROS) and gibberellin acid (GA) in regulating seed germination, diphenylene iodonium chloride (DPI) and uniconazole (Uni), as hydrogen peroxide (H2O2) and GA synthesis inhibitor, respectively, were exogenously applied on tobacco seeds using the seed priming method. Seed priming with DPI or Uni decreased germination percentage as compared with priming with H2O, especially the DPI + Uni combination. H2O2 and GA completely reversed the inhibition caused by DPI or Uni. The germination percentages with H2O2 + Uni and GA + DPI combinations kept the same level as with H2O. Meanwhile, GA or H2O2 increased GA content and deceased ABA content through corresponding gene expressions involving homeostasis and signal transduction. In addition, the activation of storage reserve mobilization and the enhancement of soluble sugar content and isocitrate lyase (ICL) activity were also induced by GA or H2O2. These results strongly suggested that H2O2 and GA were essential for tobacco seed germination and by downregulating the ABA/GA ratio and inducing reserve composition mobilization mutually promoted seed germination. Meanwhile, ICL activity was jointly enhanced by a lower ABA/GA ratio and a higher ROS concentration.
研究领域:
植物生理学
·
分子生物学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Yan Liu,Jiajia Han,Guosheng Chen,Siming Huang,Shuyao Huang,Juan Zheng,Jianqiao Xu,Fang Zhu,Gangfeng Ouyang
Phytohormones are a class of endogenous substances that separately or synergistically regulate the growth, development, and differentiation of plants. Accurately and efficiently detecting and monitoring the concentration of plant hormones in living plants is of significant importance. Herein, a novel mesoporous carbon hollow spheres (MCHS)-based in vivo solid phase microextraction (SPME) probe was designed for in vivo sampling of plant hormones. The designed MCHS features the advantages of high surface area, porous shells, and large hollow spaces, facilitating the dynamic adsorption and enrichment of target phytohormone. In addition, a cationic polyelectrolyte, (poly (diallyl dimethyl ammonium chloride) (PDDA), was further modified onto the MCHS to expedite the extraction process by electrostatic interaction. Utilizing the MCHS@PDDA probe in combination with HPLC-MS/MS facilitated the continuous monitoring of three plant hormones (abscisic acid (ABA), indole-3-acetic acid (IAA), and gibberellin (GA3)) in Chinese aloe. The detection limit of this method was 0.016–0.090 μg/L, the linear range was 10–1000 μg/L, and both the RSD of the single probe (n = 6) and probe-to-probe test (n = 6) were less than 7.2 %. This method had excellent accuracy and good reproducibility comparable to the traditional sample pretreatment method. Ultimately, this established in-vivo SPME method was successfully adopted to quantify three selected plant hormones in living Chinese Aloes, providing a new method for the long-term monitoring of endogenous active substances in living system.
研究领域:
分析化学
·
植物科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF EXPERIMENTAL BOTANY
·
2023
·
IF 6.9
He Chengyong,Xing Fei,Liang Jiahui,Zhang Zhixiang,Zhan Binhui,Habili Nuredin,Wang Hongqing,Li Shifang
Apple necrotic mosaic virus (ApNMV) is associated with apple mosaic disease in China. However, the mechanisms of ApNMV infection, as well as host defence against the virus, are still poorly understood. Mitochondrial ATP synthase plays a fundamental role in the regulation of plant growth and development. However, mitochondrial ATP synthase function in response to virus infection remains to be defined. In the present study, a yeast two-hybrid (Y2H) screening revealed that the apple mitochondrial ATP synthase oligomycin sensitivity-conferring protein (OSCP) subunit (MdATPO) interacts with ApNMV coat protein (CP). It was further verified that overexpression of MdATPO in Nicotiana benthamiana inhibited viral accumulation. In contrast, silencing of NbATPO facilitated viral accumulation, indicating that ATPO plays a defensive role during ApNMV infection. Further investigation demonstrated that ApNMV infection accelerated abscisic acid (ABA) accumulation, and ABA negatively regulated ATPO transcription, which was related to the ability of ABA insensitive 5 (ABI5) to bind to the ABA-responsive elements (ABREs) of the ATPO promoter. Taken together, our results indicated that transcription factor ABI5 negatively regulated ATPO transcription by directly binding to its promoter, leading to the susceptibility of apple and N. benthamiana to ApNMV infection. The current study facilitates a comprehensive understanding of the intricate responses of the host to ApNMV infection.
研究领域:
植物病理学
·
分子生物学
·
病毒学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANNALS OF BOTANY
·
2024
·
IF 3.6
Guo Huanhuan,Li Shiqin,Liu Yong,Yang Qinsong
Background and AimsChinese pistachio (Pistacia chinensis Bunge), an important horticultural plant species, holds great ornamental value with beautiful leaves and fruits. Seedling propagation of this tree species is restricted by its erratic seed germination, however, the germination mechanism is ambiguous yet. The aim of this study was to figure out the germination mechanism from the novel perspective based on the multi-omics data.MethodsThe multi-omics technique combined with hormone content measurement was first applied in seed germination of Chinese pistachio.Key ResultsDue to the great accumulation during seed germination, catechin stood out from the identified metabolites by broadly targeted metabolomic analysis. Exogenous catechin of 10 mg/L significantly improved the germination of Chinese pistachio seeds. An interesting result of hormone analysis showed that the improving effect of catechin could be attributed to increase of the gibberellic acid 3 (GA3) content rather than decrease of the abscisic acid (ABA) content before germination. The paclobutrazol (PAC, a GA biosynthesis inhibitor) and PAC + catechin treatments also showed that the promoting effect of catechin on seed germination depends on GA biosynthesis. Transcriptome analysis and qRT‒PCR further revealed that catechin induced the expression of PcGA20ox5 to activate GA biosynthesis. Several transcription factors were induced by catechin and GA treatments, such as TCP, bZIP and C3H, which may play an important regulatory role in GA biosynthesis in a catechin-mediated way.ConclusionsCatechin promotes seed germination via GA biosynthesis in Chinese pistachios. This study proposes a novel mechanism by which catechin promotes seed germination via the GA pathway, which provides new insight into a comprehensive understanding of seed dormancy and germination.
研究领域:
植物学
·
植物生理学
·
分子生物学
·
园艺学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Hongmei Xiang,Jiao Meng,Wubin Shao,Dan Zeng,Jin Ji,Peiyi Wang,Xiang Zhou,Puying Qi,Liwei Liu,Song Yang
The excessive use of non-renewable formulations, low utilization efficiency, and eco-unfriendly behavior are serious disadvantages of traditional pesticide formulations. Herein, a scalable and sustainable biopolymer-based core–shell (CS) nanocarrier loaded with an anti-viral candidate agent, Bingqingxiao (BQX), was investigated for plant disease management. Preliminary [email protected] @SWP nanoparticles ( [email protected] @S NPs) were manufactured through the noncovalent self-assembly of pyridine-grafted poly (hydroxyethyl methacrylate) (PyPHEMA) and natural soy whey protein (SWP), and exhibited a stable spherical morphology with a diameter of approximately 130 nm. Notably, encapsulation of BQX into the NPs increased the ultra-violet (UV) resistance of the compound by 8.7-fold, and the nanocarrier [email protected] ( [email protected] ) enhanced the BQX foliar surface wettability, resulting in a slow, urea-responsive release. In vivo studies revealed that the protective activity of [email protected] @S NPs was 1.4-fold higher than that of BQX against tobacco mosaic virus (TMV), and the antiviral efficiency was further improved by stimulation with 0.2 M urea. Furthermore, foliar application of [email protected] @S NPs activated host plant defense responses by upregulating the expression of salicylic acid (SA)- and abscisic acid (ABA)-related genes. Interestingly, the developed [email protected] @S NPs act as plant nutrition to enhance the crop fresh and dry weight by 24.7% and 19.9%, respectively. Consequently, [email protected] is a promising biocompatible carrier that can enhance the potency of various pesticides, synergistically improve plant disease resistance, and simultaneously act as a fertilizer for crop growth.
研究领域:
农业科学
·
纳米技术
·
植物病理学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Dahe Qiao,Junyan Zhu,Xiaozeng Mi,Hui Xie,Mingtao Shu,Minyi Chen,Rui Li,Shengrui Liu,Chaoling Wei
Withering time of fresh leaves is a key factor affecting the stability of flavor quality of Taiping Houkui tea (TPHK). It's effect and mechanism on the formation of flavor in TPHK remain unelucidated. Here, metabolomics and transcriptomics were combined to comprehensively investigate changes in the main flavor compounds and the expression patterns of related genes under different withering times. The results showed that withering had the greatest influence on the content of most amino acids and aroma volatiles. The relative content of most volatiles was observed to be highest at 6–8 h of withering time, which was consistent with the results from the sensory evaluation of aroma quality. Plant hormone signal transduction pathways played a key role in the variation of the flavor compound content under different withering times, especially ABA and JA signals. WGCNA demonstrated that ABA, JA, IAA, SA and different flavor substances were significantly associated with specific modules. JA may be involved in the metabolism of theanine during withering, while ABA may be involved in the accumulation of aromatic amino acids and main aroma components of TPHK. This research may serve as a reference for the optimization of the processing technology for TPHK.
研究领域:
植物学
·
遗传学
·
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Polymer Chemistry
·
2024
·
IF 4.1
Huangbing Xu,Teng Qiu,Haotian Shi,Xiaoqian Tian,Xiaoyu Li,Longhai Guo
RAFT-mediated polymerization-induced self-assembly (RAFT-PISA) has been undergoing fast development currently, promoting research toward more diverse morphologies and more cutting-edge applications. Herein, we demonstrate a polymerization-pH tailored RAFT-PISA (pH-PISA) process, which can conveniently harvest abundant nano/micro-scale morphologies using popular monomers N,N-dimethylacrylamide (DMAA) and diacetone acrylamide (DAAM). The process proceeded through ordinary RAFT dispersion polymerization in an aqueous medium but under different pH conditions. With the use of pH-sensitive bi-carboxylic acid-capped trithiocarbonate as the chain transfer agent (CTA), it was found that the PISA of propagating amphiphilic ABA triblock copolymers was highly affected by the different ionization of the CTA. When the polymerization pH was adjusted to 2.5, an especially wide spectrum of morphologies was detected, including spheres, worms, lamellae, single or double-head tadpoles, vesicle strings and large spheres with surface protrusions. However, the diversity in morphology gradually declined when the polymerization pH was increased to 6 or 8. Diverse structures with same monomer compositions provide an ideal model to explore the influence of particle morphology on ice recrystallization inhibition (IRI) activities. The variations in mean largest grain sizes (MLGS) and growth rates of a single ice crystal were related to packing parameters (P), bridging the interface geometry of the packing of amphiphilic molecules and ice-controlling activities.
研究领域:
高分子化学
·
材料科学
·
胶体与表面化学
·
低温生物学
✓ 已验证
产品关联
DOI
高影响因子
PLANT SCIENCE
·
2021
·
IF 4.729
Zhengke Zhang,Jialiang Liu,Donald J. Huber,Hongxia Qu,Ze Yun,Taotao Li,Yueming Jiang
Litchi fruit has high commercial value on the international market, but senesces rapidly after harvest. We used weighted gene co-expression network analysis (WGCNA) and degradome technology to investigate the molecular mechanisms of melatonin-mediated delay of litchi fruit senescence through application of exogenous melatonin and p-chlorophenylalanine (p-CPA, an inhibitor of melatonin biosynthesis) treatments. Results demonstrated that exogenous melatonin treatment delayed litchi fruit senescence while p-CPA accelerated senescence. Coupled analyses of transcriptome and physiological parameters of litchi fruit provided the correlation of network modules with dynamic changes in browning index during storage. Additionally, we found that microRNAs (miR858 and miR160a) and their targets were actively involved in melatonin-mediated delay of litchi fruit senescence. Melatonin treatment decreased abscisic acid (ABA) content but increased PP2C and F-box expression levels, suggesting the involvement of ABA signaling in melatonin-mediated antisenescence. The transcriptions of ZAT, NAC and DREB1 were activated by melatonin treatment. Moreover, the major functional genes involved in histone methylation, γ-aminobutyric acid (GABA) metabolism, energy production, reactive oxygen species (ROS) accumulation and cell death were identified in the melatonin-inhibited litchi pericarp browning. Taken together, we first constructed the global map of the important regulators and pathways to delay litchi senescence and pericarp browning mediated by melatonin.
研究领域:
植物学
·
遗传学
·
分子生物学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）