FITOTERAPIA
·
2019
·
IF 2.431
Fenqin Zhao,Qianqian Zhang,Yan Yan,Haiyan Jia,Xinran Zhao,Xiying Li,Lihua Zheng,Guang Han
Dendranthema morifolium cv. ‘jinsidaju’, cultivated only in Kaifeng, has been eaten for more than 1000 years. During the antioxidant-activity-guided studies on its chemistry and health care function, two new bisabolane-type sesquiterpenes , (6 R ,7 R )-7-hydroxybisabol-2,9 E ,11-triene-4-one (jinsidajuol A, 1 ) and (6 R ,7 R )-7-hydroxy-11-methoxybisabol-2,9 E -diene-4-one (jinsidajuol B, 2 ), and thirteen known compounds ( 3 – 15 ) were isolated from the flowers. Their structures were elucidated by 1D and 2D NMR spectroscopy and HRMS. 1 and 2 are the first example of bisabolane-type sesquiterpenes isolated from the genus Dendranthema. Compounds 6 – 8 , 12 and 13 exhibited strong scavenging activities on the ABTS radical cation with IC 50 3.33, 5.67, 2.00, 2.50, 5.33 μg/mL, respectively. The IC 50 values of all compounds on HepG2 human hepatoma tumor cell line were higher than 50 μM.
研究领域:
药用植物学
·
生物化学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 9
·3928
·
IF 4.6
Jiao Huang,Donglin Chen,Mengying Liu,Yarui Yu,Yi Zhang,Jing Huang
Seven new phenylhexanoids, (S)-(+)-3,4-dihydroxy-11-methoxyphenylhex-9-one (1), (E) 3,4-dihydroxy-phenylhex-10-en-9-one (2), (E)-4-hydroxyphenylhex-10-en-9-one (3), (R)-(−)-3,4,11-trihydroxyphenylhex-9-one 11-O-β-d-glucopyranoside (4), (R)-(−)-4,11-dihydroxyphenylhex-9-one 11-O-β-d-glucopyranoside (5), phenylhex-4,9,11-triol 11-O-β-d-glucopyranoside (6), and 9-O-acetyl-phenylhex-4,9,11-triol 11-O-β-d-glucopyranoside (7), were isolated and identified from Tibetan medicineSaxifraga umbellulatavar.pectinate. The antioxidant activities of these compounds were evaluated using the DPPH and ABTS radical scavenging experiments. In the ABTS experiment, compounds1(IC5013.99 ± 2.53 μM) and2(IC5013.11 ± 0.94 μM) exhibited significantly better antioxidant activity than L-ascorbic acid (IC5023.51 ± 0.44 μM).Keywords:Saxifraga umbellulatavar.pectinate;phenylhexanoid;antioxidant activity
研究领域:
药用植物学
·
天然产物化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Natural Product Communications
·
2014
·
IF 0.924
Zihao Zhou,Jun Tang,Xueping Song
The chemical composition of the essential oil from roots and rhizomes of Ligularia hodgsonii was analyzed by gas chromatography (GC) and GC-mass spectrometry (MS). Forty-seven components were identified, representing 77.0% of the total oil. The main constituents were l-(+)-ascorbic acid 2,6-dihexadecanoate (15.7%), selina-6-en-4-ol (8.4%) and 9,10-dimethyl-1,2,3,4,5,6,7,8-octahydroanthracene (6.6%). The free radical scavenging activities of the essential oil, aqueous extract, ethanolic extract, and crude polysaccharides of L. hodgsonii, as well as some of their major components, were investigated using DPPH and ABTS assays. The essential oil displayed a lower capacity to quench free radicals than the extracts; the ethanolic and aqueous extracts showed considerably higher antioxidant potential that deserves further study.
研究领域:
植物化学
·
药理学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Dandan Ren,Jie Mei,Jian Bao,Fangdi Wei,Guanhong Xu,Jing Yang,Yong Sun,Qin Hu,Yao Cen
In this work, we reported a novel and convenient profuse color card for naked eye determination of iodide (I − ) in urine using chromogenic substrate 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). I − catalyzed the oxidation of ABTS by peroxyacetic acid causing ABTS to yield cyan product ABTS + with a new absorption peak at 730 nm. The addition of rose-red dye rhodamine B (RhB) changes the overall color of the solution from pink to purple and finally to blue, which makes the solution multicolor and easy to distinguish. A good linear relationship for I − was obtained ranging from 10.0 to 500.0 μg/L with the detection limit of 9.2 μg/L. Importantly, the sensor can semi-quantitatively estimate the concentration of I − in human urine with naked eye through the standard color card and assess the deficiency or excess of iodine in human body. The proposed profuse color card opens up a new colorimetric method for the rapid, simple and reliable determination of I − in clinic, and has promising applications in developing assay kit for the clinical diagnosis of I − in urine.
研究领域:
医学诊断
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 7
·2123-2129
·
IF 4.831
Li Xue-Jiao,Wang Yong-Sheng,Yang Sheng-Yuan,Tang Xian,Liu Lu,Zhou Bin,Wang Xiao-Feng,Zhu Yu-Feng,Huang Yan-Qin,He Shun-Zhen
We report on a photometric method for the determination of the metallothioneins (MTs). It is known that citrate capped gold nanoparticles (AuNPs) coated with traces of mercury possess peroxidase-like properties that can catalyze the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline- 6-sulfonate) (ABTS) to form a blue product in acetate buffer of pH 4.5. It is found that if the AuNPs are first aggregated by the cysteine-rich metallothioneins, the peroxidase-like properties of the resulting aggregates (AuNP-Hg-MTs) cause a largely accelerated oxidation of ABTS. The effect of adding MTs to such a solution is used to quantify the MTs by a kinetic assay. Changes in absorbance at 416 nm are linearly correlated to the concentration of MTs in the 4.3 to 49 nM range, and the detection limit is 1.3 nM. The method was successfully applied to the determination of MTs in (spiked) human urine. The strategy may pave the way for related detection platforms.
The gold nanoparticle (AuNP)-Hg-metallothionein (MT) hybrid possesses a stronger peroxidase-like activity than the AuNP-Hg complex. It can catalyze H 2 O 2 -mediated oxidation of ABTS along with the change of the absorbance of the assay system, which is directly proportional to the concentration of MTs in the linear range.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2026
·Vol. 40
·期 7
·e70526
·
IF 1.7
Shu-Zhen Chen,Yong-Lin Ma,Dong-Xiao Li,Lian-Lin Yang,Ji-Wen Wang,Qian-Qian Hu,Hong-Liang Ma
This study developed an online HPLC-ABTS method to screen antioxidant phenolic acids in Pandani tectorii radix et rhizoma (PT) from 20 geographical origins. Six common constituents were screened and assigned by standards: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C. Using these six components as variables, four regression models were constructed to predict ABTS radical scavenging rate: support vector regression (SVR), decision tree regression, K-nearest neighbors (KNN) regression, and extreme gradient boosting (XGBoost) regression. From 80 samples, 64 were used for model development, and 16 were reserved for hold-out validation. Model selection employed 5-fold, nested, and leave-one-out cross-validations, complemented by permutation tests and learning curve analysis. The SVR model performed best, with nested CV R 2 = 0.9298, RMSE = 4.1207, MAE = 2.2313, and an optimism gap of 0.0137. After final tuning (C = 100, gamma = 0.1, and epsilon = 0.5), it achieved R 2 = 0.9000, RMSE = 4.0602, and MAE = 2.2986 on the hold-out set. This efficient and accurate method enables simultaneous screening of antioxidant constituents and prediction of radical scavenging activity, providing a new technical tool and scientific basis for PT quality evaluation.
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY OF NATURAL COMPOUNDS
·
2020
·Vol. 56
·期 5
·821-826
·
IF 0.653
Shao Jin,Zhao Tong,Ma Hui-Ping,Jia Zheng-Ping,Jing Lin-Lin
A convergent synthesis route of 6-hydroxygenistein (6-OHG) was reported, starting from cheap and readily available biochanin A, via methylation, bromination, methoxylation, and demethylation. The structure of the products was confirmed by MS, IR, 1 H NMR, and 13 C NMR analysis. The antiradical activity of 6-OHG was determined using six different methods, namely, DPPH assay, ABTS assay, nitric oxide assay, superoxide assay, reducing power assay, and phosphomolybdenum assay using ascorbic acid (V C ) as positive control. The results show that 6-OHG possesses similar or greater antiradical activity than V C . In conclusion, 6-OHG can be useful as an antioxidant agent.
研究领域:
有机化学
·
药物化学
·
天然产物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
L.X. Chen,D.J. Hu,S.C. Lam,L. Ge,D. Wu,J. Zhao,Z.R. Long,W.J. Yang,B. Fan,S.P. Li
Snow chrysanthemum ( Coreopsis tinctoria Nutt.), a world-widely well-known flower tea material, has attracted more and more attention because of its beneficial health effects such as antioxidant activity and special flavor. In this study, a high performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) and 2,2′-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt (ABTS) based assay was employed for comparison and identification of antioxidants in different samples of snow chrysanthemum. The results showed that snow chrysanthemum flowers possessed the highest while stems presented the lowest antioxidant capacities. Fourteen detected peaks with antioxidant activity were temporarily identified as 3,4′,5,6,7-pentahydroxyflavanone-O-hexoside, chlorogenic acid, 2R-3′,4′,8-trihydroxyflavanone-7-O-glucoside, flavanomarein, flavanocorepsin, flavanokanin, quercetagitin-7-O-glucoside, 3′,5,5′,7-tetrahydroxyflavanone-O-hexoside, marein, maritimein, 1,3-dicaffeoylquinic acid, coreopsin, okanin and acetyl-marein by comparing their UV spectra, retention times and MS data with standards or literature data. Antioxidants existed in snow chrysanthemum are quite different from those reported in Chrysanthemum morifolium , a well-known traditional beverage in China, which indicated that snow chrysanthemum may be a promising herbal tea material with obvious antioxidant activity.
研究领域:
植物化学
·
食品科学
·
药用植物研究
✓ 已验证
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 8
·1733
·
IF 4.2
Shuo Zhang,Ze Liu,Xinyu Xu,Ruihua Zhao,Shujiang Zhang,Rong Luo
In traditional Chinese medicine, Aurantii Fructus Immatures (AFIs) have been utilized for more than 2000 years. The proportions of different fruit parts are crucial for evaluating AFI quality in China. However, the basis for this statement’s substance is unclear. Differences in quality are intimately correlated with a plant’s metabolite composition. On the basis of a widely targeted metabolome, this study intended to investigate the metabolite composition and evaluate the antioxidant capacity of the peel and pulp of an AFI. Metabolites were identified and quantified by UHPLC-QqQ-MS. To assess their antioxidant ability, DPPH and ABTS assays were carried out. There were 1327 chemical compounds identified by UHPLC-QqQ-MS. After screening the differential metabolites using a multivariate statistical analysis, it was found that there were 695 significant differences in the metabolites between the peel and the pulp. Among them, it was discovered that the content of active ingredients in the peel group was higher than that in the pulp group. Furthermore, the aqueous extracts from the peel showed stronger antioxidant capacities than those from the pulp. The metabolites and antioxidant capacities were significantly different between the peel and the pulp. This study of different fruit parts might provide a guide for AFI quality assessments.
研究领域:
药理学
·
中药学
·
代谢组学
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2023
·
IF 2.9
Dongyu Gu,Shanshan Tang,Chang Liu,Dajun He,Jing Tian,Yi Yang
Coprinus comatus is an edible and medicinal fungus. In this study, the antioxidant activity of the fermentation product of C. comatus was investigated through optimization of fermentation process. The results indicated that the fermentation product of C. comatus had obvious scavenging ability for 2,2’-Azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) free radical. The EC50 of the n-butanol extract from the fermentation product on ABTS·+ was 0.65 ± 0.02 mg/mL. On this basis, the liquid fermentation conditions of C. comatus were optimized through single factor and response surface optimization experiments according to the scavenging ability of ABTS·+ to improve the antioxidant capacity of the fermentation product. The results showed that when the 14% of C. comatus was fermented in a culture medium with a C/N ratio of 48:1 for 6 days, the ABTS·+ scavenging ability was the strongest, and the EC50 of n-butanol extract was 0.57 ± 0.01 mg/mL, which was 12.31% higher than the initial activity. This study laid the foundation for the development of C. comatus.
研究领域:
微生物学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2014
·Vol. 8
·期 6
·1556-1564
·
IF 1.802
Liu Tao,Zhang Xiao,Hao Jianpeng,Zhu Wenxin,Liu Wei,Zhang Daohong,Wang Jianlong
Herein, we for the first time report an acetylcholinesterase (AChE)-free colorimetric method for the detection of chlorpyrifos (CP), which is based on the oxidation reaction of H 2 O 2 with CP and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS 2− ) catalyzed by hemin/G-quadruplex DNAzyme. In the absence of CP, ABTS 2− is directly oxidized by H 2 O 2 with hemin/G-quadruplex DNAzyme as a biocatalyst to produce the blue-green-colored free radical anion (ABTS ·− ), accompanied with an obvious UV-visible absorbance at 418 nm. However, the amount of H 2 O 2 used in H 2 O 2 -DNAzyme-ABTS 2− system will be decreased if CP is first reacted with H 2 O 2 , leading to the decrease of the amount of ABTS ·− and a change in the UV-visible absorbance of ABTS ·− at 418 nm. Thus, an indirect dependence relation between CP concentration and the absorbance of ABTS ·− at 418 nm could be constructed and used for CP detection. Under optimal conditions, the detection range of the method is 0.04–1 μg/mL, with a detection limit of 0.01 μg/mL. Application of the method in real juice samples shows good analytical features in terms of recoveries (95–107.5 %), the relative standard deviation (RSD) (0.6–4.7 %), rendering it as an attractive candidate to current methodologies for the determination of CP.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 6
·2446
·
IF 4.6
Dan Wu,Qile Xia,Huilin Huang,Jinhu Tian,Xingqian Ye,Yanbin Wang
When the total phenolic content (TPC) and antioxidant activity of sea buckthorn juice were assayed by spectrophotometry, the reaction solutions were not clarified, so centrifugation or membrane treatment was needed before determination. In order to find a suitable method for determining TPC and antioxidant activity, the effects of centrifugation and nylon membrane treatment on the determination of TPC and antioxidant activity in sea buckthorn juice were studied. TPC was determined by the Folin-Ciocalteau method, and antioxidant activity was determined by DPPH, ABTS, and FRAP assays. For Treatment Method (C): the sample was centrifuged for 10 min at 10,000 rpm and the supernatant was taken for analysis. Method (CF): The sample was centrifuged for 10 min at 4000 rpm, filtered by Nylon 66 filtration membranes with pore size of 0.22 μm, and taken for analysis. Method (F): the sample was filtered by Nylon 66 filtration membranes with pore size of 0.22 μm and taken for analysis. Method (N): after the sample of ultrasonic extract solution reacted completely with the assay system, the reaction solution was filtered by Nylon 66 filtration membranes with pore size of 0.22 μm and colorimetric determination was performed. The results showed that centrifugation or transmembrane treatment could affect the determination of TPC and antioxidant activity of sea buckthorn juice. There was no significant difference (p> 0.05) between methods (CF) and (F), while there was a significant difference (p< 0.05) between methods (C) (F) (N) or (C) (CF) (N). The TPC and antioxidant activity of sea buckthorn juice determined by the four treatment methods showed the same trend with fermentation time, and the TPC and antioxidant activity showed a significant positive correlation (p< 0.05). The highest TPC or antioxidant activity measured by method (N) indicates that method (N) has the least loss of TPC or antioxidant activity, and it is recommended for sample assays.Keywords:DPPH;ABTS;FRAP;centrifugation;transmembrane
研究领域:
食品科学
·
分析化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）