JOURNAL OF FLUORESCENCE
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2025
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IF 3.1
Xue Weijian,Ling Xiangyu,Li Huiqian,Liu Yuhang,Zhao Bing,Yin Yanbing
In this work, two chain aromatic hydrocarbon derivatives 1 – 2 , which are constructed by linking two 3 H-imidazo[4,5- b ]pyridine moieties through phenyls and oxygen bridges, were designed and synthesized. The aggregation and disaggregation behavior of these sensors could be modulated by pH, as evidenced by H 1 NMR, fluorescence spectrum and DLS, attributable to the altered deprotonation of the 3 H-imidazo[4,5- b ]pyridine cores. An increase in the sensor concentration leads to the reemergence of aggregation, correspondingly diminishing the fluorescence intensity. Polyaromatic aromatic hydrocarbons (PAHs), known for their luminescent characteristics, demonstrate sustained luminescence even at high concentrations of 10 − 3 M. Notably, the fluorescence of PAHs are effectively quenched upon mixed with 7 × 10 − 5 M aggregated sensor 1 or 10 − 4 M aggregated sensor 2 . Aggregation is identified as the predominant mechanism for fluorescence quenching, the finding corroborated by fluorescence spectroscopy and DLS measurements. Based on computational studies and mechanistic evaluations, sensors 1 and 2 emerge as promising candidates for serving as efficient fluorescence quenchers for polycyclic aromatic hydrocarbons. Graphic
研究领域:
化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xinyao Su,Jia Liu,Jiarou Liu,Qiang Xue,Caixia Wang
Melia azedarach L. serves as an important source of timber and bioactive triterpenoids, notably tetracyclic limonoids and pharmacologically active pentacyclic triterpenes such as β-amyrin derivatives. Although limonoid biosynthesis has been extensively characterized, the enzymatic machinery governing pentacyclic triterpene formation remains poorly defined. Through transcriptomic mining of M. azedarach , we identified three CYP88A subfamily cytochrome P450 genes ( MaCYP88A108 , MaCYP88A164 , and MaCYP88A222 ). Functional characterization in both Nicotiana benthamiana transient expression systems and yeast microsomes demonstrated their catalytic roles as β-amyrin 11-oxidases, sequentially converting β-amyrin to 11-oxo-β-amyrin via C-11 oxidation. Intriguingly, MaCYP88A108 and MaCYP88A164 exhibited dual functionality, participating not only in pentacyclic triterpene production but also in limonoid biosynthesis, suggesting their evolutionary recruitment for triterpenoid diversification. Phylogenetic reconstruction and structural modeling revealed that these CYP88A enzymes share close homology and conserved active-site architectures with Glycyrrhiza uralensis CYP88D6 (GuCYP88D6), a characterized β-amyrin 11-oxidase, despite lacking genomic synteny. This observation supports a paradigm of functional convergence driven by structural conservation rather than shared gene lineage. Furthermore, we characterized an oxidosqualene cyclase ( MaβAS1 ) responsible for cyclizing 2,3-oxidosqualene to β-amyrin, thereby establishing the substrate pool for downstream oxidation. Our study uncovers a unique catalytic versatility in plant CYP88A P450s, demonstrating their capacity to coordinate two divergent triterpenoid branches. This functional plasticity, mediated by evolutionarily conserved structural frameworks, expands current paradigms of enzyme multifunctionality in plant specialized metabolism. The identified genes ( CYP88As and MaβAS1 ) establish critical tools for synthetic biology platforms targeting heterologous production of pharmaceutically important triterpenoids, bridging mechanistic exploration of plant secondary metabolism with biotechnological applications.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2022
·Vol. 20
·期 1
·1-13
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IF 9.429
Luo Zhenyu,Luo Lihua,Lu Yichao,Zhu Chunqi,Qin Bing,Jiang Mengshi,Li Xiang,Shi Yingying,Zhang Junlei,Liu Yu,Shan Xinyu,Yin Hang,Guan Guannan,Du Yongzhong,Cheng Ningtao,You Jian
Adoptive cell therapy (ACT) was one of the most promising anti-tumor modalities that has been confirmed to be especially effective in treating hematological malignancies. However, the clinical efficacy of ACT on solid tumor was greatly hindered by the insufficient tumor-infiltration of cytotoxic CD8 + T cells. Herein, we constructed a nanoplatform termed dual-binding magnetic nanoparticles (DBMN) that comprised PEG-maleimide (Mal), hyaluronic acid (HA) and Fe3O4 for adoptive T cell-modification and ACT-sensitization. After a simple co-incubation, DBMN was anchored onto the cell membrane (Primary linking) via Michael addition reaction between the Mal and the sulfhydryl groups on the surface of T cells, generating magnetized T cells (DBMN-T). Directed by external magnetic field and in-structure Fe3O4, DBMN-T was recruited to solid tumor where HA bond with the highly expressed CD44 on tumor cells (Secondary Linking), facilitating the recognition and effector-killing of tumor cells. Bridging adoptive T cells with host tumor cells, our DBMN effectively boosted the anti-solid tumor efficacy of ACT in a mouse model and simultaneously reduced toxic side effects.
研究领域:
癌症研究
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纳米技术
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免疫疗法
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生物医学工程
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DOI
PubMed
高影响因子
Environmental Science-Nano
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2025
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IF 5.1
Zhongmiao Wang,Jie Hou,Jiang Xu,Kun Yang,Daohui Lin
Microorganisms are pivotal in decomposing persistent organic pollutants in the environment. However, the bioavailability of pollutants is often hindered by their strong adsorption to ubiquitous fine colloids. This study provides the first evidence that Rhodococcus biphenylivorans, a known intracellular oxidative degradation bacterium, can reductively degrade organochlorine pollutants adsorbed on biochar colloids (BCCs) extracellularly, besides the oxidative degradation of BCC-adsorbed pollutants intracellularly. Over a 30 day biodegradation period, the total biodegradation rates of adsorbed 2,4,4′-trichlorobiphenyl (PCB28, 10 mg L−1) on BCCs (1 g L−1) prepared at pyrolysis temperatures of 300, 500, and 700 °C were 59.4%, 34.8%, and 10.4%, respectively. The biodegradation products of adsorbed PCB28 on low-temperature BCCs were mainly chlorosubstituted-2-hydroxy-6-oxo-6-phenyl-2,4-hexadienoic acid-type products, while in the high-temperature BCC system, dichlorobiphenyl was the main product. Mechanistically, BCCs with low pyrolysis temperature could fragment during the degradation, facilitating the transport of adsorbed PCB28 into bacteria for the intracellular oxidative degradation; the interactions between bacterial cells and BCCs (especially with high pyrolysis temperature) could stimulate the respiratory chain electron efflux for the extracellular dechlorination degradation. These findings not only provide new insights into the modulatory influence of BCCs on microbial degradation of pollutants, but also enrich our knowledge on the environmental fate of colloid-adsorbed pollutants.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
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2022
·
IF 8.129
Wei Ruan,Haoyun Liu,Hao Wu,Yuan Qi,Mengchang Zhou,Changsong Zhou,Zhen Zhang,Hongmin Yang
In this work, the Zr-based MOF was successfully fabricated with 4,6-Diamino-2-mercaptopyrimidine (DMP) and a novel adsorbent (Uio-DMP) was synthesized for the immobilization of Pb 2+ in aqueous medium . The morphological and structural characteristics of Uio-DMP were characterized by X-ray diffractometer (XRD), Fourier transform infrared (FT-IR), Field emission scanning electron microscopy (FE-SEM), Brunner Emmet Teller (BET), and X-ray photoelectron spectroscopy (XPS). The characterization results showed that the Uio-DMP maintained a pristine crystal structure and the sulfhydryl group was introduced to Zr-based MOF after chemical functionalization. The adsorption behavior of Uio-DMP for Pb 2+ was investigated in different conditions (solution pH, sorbent dose, Pb 2+ concentration, adsorption time, and co-existing ions). The experimental results indicated that the optimal pH was 6, the maximum adsorption capacity was 296.5 mg/g, and the Langmuir model well fitted the Pb 2+ removal process based on adsorption isotherm studies. The adsorption kinetic experiments revealed that the adsorption equilibrium could be achieved at 30 min and the adsorption process was consistent with the Pseudo-second-order model. The co-existing ions had a limited impact on Pb 2+ removal and the removal rate of 71.0% was maintained after five consecutive adsorption-desorption experiments. Furthermore, zeta potential and XPS analysis proved that the removal process of Pb 2+ over Uio-DMP was affected by electrostatic interaction , and nitrogen/sulfur atoms acting as the main adsorption sites. This study provides an insight into the removal of Pb 2+ in aqueous medium and the functionalization of adsorbents.
研究领域:
环境科学
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材料科学
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化学工程
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DOI
高影响因子
Materials Today Chemistry
·
2023
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IF 7.3
L. Wang,D. Wang,Y. Tao,J. Wang,M. Tian,Q. Liu,Y. Yang,Y. Zou,F. Ke,X. Guo,X. Li,D. Gao
Phenylboronic acid functionalized high-crystallinity fluorescent covalent organic framework ( [email protected] ), which can be applied for rifamycin antibiotics and water sensing, and for rifamycin antibiotics adsorption, was synthesized for the first time. To effectively form [email protected] by post-synthetic modification, a fluorescent COF (COF–OMe) was prepared using deep-eutectic solvent as solvent without using catalyst. The [email protected] showed good fluorescence property and adsorption capacity, which owned superior effects in detecting and adsorbing rifampicin, rifapentine, and rifabutin. The results of fluorescence quenching mechanisms showed that internal filtration effect, dynamic quenching, and photoinduced electron transfer were the major mechanisms. Moreover, mainly due to the pore selectivity of [email protected] , hydrogen bonding, and π-π interaction, [email protected] showed good selectivity for three antibiotics. Finally, a dual-function platform based on [email protected] was successfully developed for sensing and adsorbing three antibiotics from various samples. Besides, [email protected] also showed good effect to detect water amount in ethanol (0.1−50%, v/v).
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BIOSENSORS & BIOELECTRONICS
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2023
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IF 12.6
Jun Wang,Jiawen Han,Juan Wang,Xujuan Lv,Daoqing Fan,Shaojun Dong
Versatile nanozymes with fascinating catalytic properties provide inspiring and effective options for biosensing and pharmaceutical analysis. Herein, we report the first nanozyme-based ratiometric fluorescent platform for cysteine (Cys) and bleomycin (BLM) detection by harnessing the cost-effective and “mix & act” G-quadruplex/Cu(II) (G4/Cu) metal-nanozyme with satisfactory peroxidase-like activity, which was fully proven by circular dichroism (CD), electron paramagnetic resonance (EPR) spectra and reactive oxygen species (ROS) scavenging experiments. Based on the catalytic oxidation of G4/Cu metal-nanozyme toward two fluorescent substrates (Amplex Ultrared, AU; Scopoletin , Sc) with opposite responses in the presence of H 2 O 2 , and the specific interaction between Cu 2+ and targets, we achieved the highly sensitive detection of Cys and BLM. Through recording the fluorescence changes of AU (emission at 590 nm, F590) and Sc (emission at 465 nm, F465), we obtained good linear relationships between ratiometric fluorescence values (F590/F465) and variable contents of targets, resulting in the competitive LODs of Cys (6.7 nM) and BLM (10 nM), respectively. Moreover, this platform presented high selectivity (without the need for masking agent) and acceptable performance in human serum samples. Furthermore, a library of DNA contrary logic pairs (CLPs) and multilevel concatenated circuits were fabricated based on the reverse dual-output of the above platform, enriching the building blocks of biocomputing. This work not only enlightened the design of affordable, “mix & act” type nanozyme-based ratiometric biosensors with high reliability, but also facilitated the pluralistic application of nucleic acid-templated nanozymes to innovative biocomputing.
研究领域:
生物传感
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药物分析
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生物计算
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DOI
PubMed
高影响因子
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