ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Xingda Song,Rongbin Nie,Xiaoxia Liu,Yiping Chen,Li Yang
We proposed an innovative surface modification-mediated porous layer open tubular (PLOT) capillary, which was modified via an in situ biphasic reaction. This capillary comprised three-dimensional homogeneous and porous structures, which could increase the surface-area-to-volume ratio for antibody immobilization. The PLOT capillary was shown as an ideal immunoreaction base to enhance the sensitivity of immunoassays and shorten analysis time. By connecting two separate PLOT capillaries using a suitable sleeve tube, we can perform multiplex targets detection in the same sample. We developed a sensitive, rapid, and multiplex PLOT capillary-mediated immunosensor for the simultaneous identification of alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA) in clinical serum samples with good accuracy. The detection sensitivity of the PLOT immunosensor improved by 10-fold compared with that of bare-capillary sensor, and the whole analysis could be completed within 1 h. This work suggest that suitable surface modification strategy is an effective tool to improve the analytical performance of conventional immunoassay and our study provided a feasible, sensitive, and multi-target assay for the detection of cancer biomarkers, which would be of valuable application in clinical diagnosis.
研究领域:
分析化学
·
生物医学工程
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 5
·526-532
·
IF 2.073
Jing Wang,Dongcheng Yang,Mifa Chen,Beifang Liu,Hou Chen,Hui Xu,Wenxiang Wang,Liangjiu Bai
A novel strategy based on nanoprobes for signal amplification was introduced in an electrochemical immunoassay platform. The nanoprobes based on poly(2-hydroxyethyl methacrylate)-r-poly(acrylic acid) (PHEMA-r-PAA) and graphene oxide (GO) have the effect of double signal amplification. The resulting sandwich-type electrochemical immunosensor was able to detect the tumor marker alpha fetal protein (AFP) with high sensitivity and high selectivity. A good linear relationship could be observed in the AFP concentration range of 3.5 pg mL−1 to 35 ng mL−1 (R2 = 0.9898), and the minimum detection limit was 0.106 pg mL−1. In addition, the electrochemical immunosensor exhibited good stability and could be applied to AFP detection in clinical serum.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
癌症研究
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产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Yaran Zhang,Kebing Yi,Feng Gong,Ziwen Tang,Yu Feng,Yilong Tian,Ming Xiang,Fuxiang Zhou,Min Liu,Xinghu Ji,Zhike He
Alpha-fetoprotein (AFP), as a tumor marker, plays a vital role in the diagnosis of liver cancer. In this work, a novel sandwich immunoassay based on a thermosensitive polymer, poly(N-isopropylacrylamide) (PNIPAM), was developed for the detection of AFP. This immunoassay could realize one-step rapid reaction within 1 h, and facilitate the separation of the target molecules by incorporating PNIPAM. In this method, a conjugate of PNIPAM and capture antibody (Ab1) was successfully synthesized as a capture probe and the synthetic method of PNIPAM-Ab1 was simple, while the detection antibody (Ab2) was labeled with fluorescein isothiocyanate (FITC) to form a fluorescent detection probe. By employing a sandwich immunoassay, the method achieved quantitative determination of AFP, exhibiting a wide linear range from 5 ng/mL to 200 ng/mL and a low detection limit of 2.44 ng/mL. Furthermore, it was successfully applied to the analysis of spiked human serum samples and the screening of patients with hepatic diseases in clinical samples, indicating its potential application prospect in the diagnosis of liver cancer.
研究领域:
生物技术
·
医学诊断
·
高分子科学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Dan Zhao,Juan Li,Chuanyun Peng,Shuyun Zhu,Jian Sun,Xiurong Yang
Inspired by the special reducing capability of ascorbic acid (AA), ascorbic acid 2-phosphate (AA2P) has been extensively utilized as a substrate in current alkaline phosphatase (ALP) activity assays owing to the ALP-triggered transformation of AA2P into AA. However, such assays usually require AA-related complicated and laborious synthesis and/or signal generation procedures. Herein, we report an interesting in situ fluorogenic interaction between o-phenylenediamine (OPD) and AA, which inspires us to put forward a novel and simple AA2P/OPD-participated fluorescence turn-on ALP activity assay for the first time, and then the corresponding ALP-based fluorescence enzyme-linked immunosorbent assay (ELISA) has also been developed by means of the conventional ELISA platforms. According to the convenient and facile detection process with clear response mechanism, our fluorogenic reaction-based assay exhibits good sensitivity, selectivity, and excellent sensing performance, which ensures fluorescence ELISA to potentially be applied in clinical diagnosis by employing a well-studied biomarker of hepatocellular carcinoma, α-fetoprotein (AFP) as the model analyte. Such original ELISA via in situ formation of fluorophore from scratch gives a new sight to develop other potential immunoassay platforms in early clinical diagnosis by controlling the target antigens in the near future.
研究领域:
生物医学工程
·
分析化学
·
临床诊断
·
免疫学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Huinan Chen,Jingyao Song,Yuanyuan Li,Dongmei Deng,Yuchen Song,Xiaoli Zhu,Liqiang Luo
Tumor biomarkers are a kind of important biomolecules for aiding cancer diagnosis and assessing tumor efficacy, among which alpha-fetoprotein (AFP) is a crucial tumor biomarker for the early diagnosis of hepatocellular carcinoma. In this study, a novel cascade signal amplifying strategy was designed for the ultrasensitive detection of AFP by DNAzyme cleaving-mediated hybridization chain reaction (HCR). The primary antibody was immobilized on the Au nanoparticles modified glassy carbon electrode, which subsequently captured AFP and the secondary antibody. Then, streptavidin was combined with the biotin-modified secondary antibody and Cu 2+ -specific DNAzyme. When Cu 2+ was present, the substrate strand of the DNAzyme would cleave and produce an ssDNA end. Finally, HCR was triggered to form long double-stranded DNA on the electrode, which would greatly inhibit the charge transfer at the electrode/solution interface. Therefore, the DNAzyme cleaving-mediated HCR strategy can be applied as cascade signal amplification for the ultrasensitive electrochemical determination of tumor biomarker AFP. Under optimal conditions, the immunosensor exhibits a broad linear range of 0.001−100 ng·mL −1 (R 2 = 0.990) and a low detection limit of 15.8 fg·mL −1 . The proposed sensor displays high sensitivity and selectivity for AFP detection, which can be employed as a universal sensing platform in clinical diagnostics.
研究领域:
生物医学工程
·
分析化学
·
癌症诊断
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 5
·1-9
·
IF 5.479
Li Guiyin,Zeng Junxiang,Liu Huiling,Ding Ping,Liang Jintao,Nie Xinmin,Zhou Zhide
Alpha-fetoprotein (AFP) is a reliable clinical marker of hepatocellular carcinoma (HCC). A highly sensitive fluorometric aptamer nanoprobe is described for AFP detection. It is based on fluorescence resonance energy transfer (FRET) between AFP aptamer labelled with 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). The PdNPs quench the green fluorescence of the FAM-AFP aptamer via interactions between nitrogen functional groups of the AFP aptamer and PdNPs. When AFP was introduced into the FAM-AFP aptamer-PdNPs FRET system, the AFP aptamer preferentially combines with AFP. This results in a conformational change and weakens the interaction between the aptamer and the PdNPs. Thus, the fluorescence of FAM recovers. The fluorescence recovery of FAM increases linearly in the 5.0–150 ng·mL −1 AFP concentration range and has a 1.4 ng·mL −1 detection limit. The assay was applied to the analysis of spiked diluted human serum. The recovery values ranged from 98.3 to 112.9%, with relative standard deviations of <1.1%. This biosensing strategy provides a reliable and ultrasensitive protocol for the quantification of biomarkers with relevant antigens and aptamers.
Schematic presentation of a fluorometric aptamer nanoprobe for AFP assay based on fluorescence resonance energy transfer (FRET) between AFP aptamer labelled with 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs).
研究领域:
生物化学
·
纳米技术
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癌症研究
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 13
·6943-6952
·
IF 4.682
Zhang Yongjun,Zhao Xiaoyu,Zhu Qi,Cheng Mingyu,Xu Wei,Wang Yaxin,Zhu Aonan,Gao Renxian,Zhang Jian,Zhang Xiaolong
As an important marker of Hepatocellular carcinoma (HCC), AFP-L3 has attracted attention. In this study, the surface-plasmon-assisted Ag nanoparticles symmetrical growth technology under magnetic field is used to fabricate the six-nanohole nanojar array containing Ag nanoparticles as the sensing substrate. The displacement of SERS signal of the optimized structure is used to detect HCC marker AFP-L3. This method has good stability, high accuracy and has great potential in the early diagnosis of HCC.
研究领域:
纳米技术
·
癌症诊断
·
生物物理
·
材料科学
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产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2021
·Vol. 37
·期 12
·1695-1700
·
IF 2.081
Nailong GAO,Jianguo CHANG,Peng DAI,Ziming ZHU,Hui YOU
Alpha-fetoprotein (AFP) is an important disease biomarker, relating to cancers such as hepatocarcinomas and gastric cancer. However, traditional methods are time-consuming, relied on bulky instruments and trained professionals, cannot satisfy the demand for low cost and point-of-care testing (POCT). In this study, a power-free POCT device was developed for the rapid and low-cost detection of AFP via one-sampling. Based on the principle of sandwich immunofluorescence, the chip is capable of automatically accomplishing on-chip mixing, labeling and capturing procedures, which only require that operator add 40 μL sample into the chip one time. The proposed device is capable of sensitively detecting human AFP in FBS with a dynamic range of 10 – 1000 ng/mL and LOD (1.88 ng/mL) within a short time of 3 min. Predictably, our method holds a great potential to be applied in the POC diagnostics of proteins, especially for some regions that are resource-limited.
研究领域:
生物医学工程
·
癌症研究
·
即时诊断
·
微流控技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Shibo Zhang,Wanchao Zuo,Pengcheng Wu,Xiangming Meng,Caixia Sun,Donghui Zhang,Jianjun Dai,Yanmin Ju
It is of great significance for the early diagnosis of cancer to detect the tumor biomarkers in human blood. However, it is still challenging to develop easily available and cost-effective methods to detect the ultralow-level tumor biomarkers. In this work, an innovative signal amplification strategy was developed for the ultrasensitive detection of tumor biomarkers. Amphiphilic peptides with high self-assembly performance can self-assemble into regulable nanofibers and enrich fluorophore to amplify the signal, which can improve the sensitivity of detection. Meanwhile, the design of MOFs with higher fluorescence quenching efficiency and luminescence characteristics can realize double-signal output and further improve the accuracy and sensitivity of detection. Taking alpha-fetoprotein (AFP) as a model tumor biomarker, this strategy achieved a wide linear range of 1–40 ng/mL detection, reducing the limit of detection (LOD) from 9.02 ng/mL to 0.28 ng/mL compared with no signal amplification strategy. Significantly, the results of clinical samples detected by this strategy was consistent with chemiluminescence immunoassay used in clinical practice. Our work provides a brand-new idea for signal amplification strategy and shows an important application prospect in the early diagnosis of various diseases.
研究领域:
生物传感器
·
癌症诊断
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
BioChip Journal
·
2021
·Vol. 15
·期 3
·268-275
·
IF 3.494
Gao Nailong,Chang Jianguo,Zhu Ziming,You Hui
Sufficiently long reaction time and low flow velocity are crucial for capillary microfluidic immunoassay chips, especially for truly “one-step” detection with continuous on-chip labelling and capturing. In this study, a multistory stairs-based new delay valve is used to meet the time and flow requirements for protein testing. The delay strategy is capable of slowing down the flow of liquid into the functional region and delaying the time out of the regions as well, thus extending the labelling and capture time to about 100 and 60 s respectively, and further reducing the flow velocity to below 8 μL/min. Based on the principle of sandwich-like immunocomplex, the designed chip is capable of automatically accomplishing on-chip mixing, labelling and capturing procedures via once sample loading and sensitively detecting human Alpha-Fetoprotein (AFP) in 40 μL serum with a wide dynamic ranging from 10 ng/mL to 10 000 ng/mL within 5 min. It could be foreseeable that this microchip has great potential in biomarkers clinical testing.
研究领域:
生物医学工程
·
微流控技术
·
即时诊断
·
生物标志物检测
·
免疫分析
✓ 已验证
DOI
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 7
·2640-2645
·
IF 7.367
Lin Yang,Ming Xuan Gao,Lei Zhan,Min Gong,Shu Jun Zhen,Cheng Zhi Huang
Early detection of cancer is helpful for the control and prevention of diseases. Due to the low content of cancer biomarkers in the early disease phases, however, an ultrasensitive and selective method is critical. In this contribution, an ultrasensitive surface-enhanced Raman scattering (SERS) immunoassay is newly developed with the principle of introducing a common enzyme-induced deposition (EID) reaction to coat a silver layer on the surface of gold nanoparticles and to form a core–shell nanostructure of Au@Ag. By using alkaline phosphatase (ALP) to dephosphorylate its substrate, 2-phospho-L-ascorbic acid trisodium salt (AAP), to form vitamin C, silver ions could be reduced into silver atoms and coated on the surface of the AuNPs; a greatly enhanced SERS signal was then obtained. As a proof of concept, α-fetoprotein (AFP) was detected as a target, which is a biomarker of liver cancer. Excellent analytical performance of the SERS immunoassay could be achieved in the range from 0.5 to 100 pg mL−1 with a limit of detection of 0.081 pg mL−1 (3σ). Identical results could be obtained by using the newly proposed SERS immunoassay for the clinical detection of AFP in serum samples of patients to those clinically obtained by chemiluminescence immunoassays, demonstrating the potential applications of the developed method in clinical diagnosis.
研究领域:
生物技术
·
纳米技术
·
分析化学
·
癌症研究
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shuo Li,Yawen Guan,Yunjie Li,Yuzhuo Tuo,Yakun Luo,Liang Si,Yingjie Hua,Fang Chai
As an early multi-purpose tumor marker of hepatocellular carcinoma, alpha-fetoprotein (AFP) plays a vital role in early diagnosis and treatment. To achieve the early and accurate determination of AFP, the POMOF was fabricated by embedding H 3 PW 12 O 40 (PW 12 ) into UIO-66-NH 2 , further immobilized on reduced GO (rGO) and fabricated an innovative POMOF nanocomposite (PW@UIO-Au/rGO) as an electrochemical immunosensor (ECI-sensor). The PW@UIO-Au/rGO achieved 17-fold signal enhancement owing to their synergistic effect, enabling PW@UIO-Au/rGO exhibit high oxidase-like catalytic activity, facilitating their sensing performance. Under optimal experimental conditions, the proposed PW@UIO-Au/rGO ECI-sensor presented excellent sensing performance over a wide range from 0.01 ng mL −1 to 500 ng mL −1 with ultra-low detection of 4.0 pg mL −1 . Notably, sensing results in real serum samples were verified by the clinical enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence immunoassay (ECL) methods with excellent accuracy and consistency, indicating the excellent environmental tolerance of the proposed ECI-sensor. This work provided a promising strategy for designing feasible ultra-sensitive probe for sensing AFP in clinical test.
研究领域:
分析化学
·
生物材料科学
·
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）