FOOD CHEMISTRY
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2025
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IF 9.8
Hongfei Du,Huang Zhang,Zixin Zhang,Zihan Yuan,Maiquan Li,Lei Zhou,Yan Liu,Aihua Lou,Mei Li,Ye Jiao,Qingwu Shen,Chaoyi Xue,Wei Quan,Lan Mo
The growing health consciousness of consumers has led the food industry to embrace healthier cooking methods, with air frying becoming increasingly popular in households. This study investigated the dual role of 10 hydrocolloids in reducing the formation of heterocyclic amines (HAs) and advanced glycation end products (AGEs) in air-fried beef patties while preserving physicochemical and sensory qualities. Marination with hydrocolloids at concentrations of 0.1–1.0 % mitigated lipid/protein oxidation, retained moisture, improved texture, and significantly inhibited HA (26.3–55.4 %) and AGE (21.3–51.1 %) formation. Among the hydrocolloids, chitosan (0.5 % and 1.0 %) demonstrated optimal efficacy, reducing HAs and AGEs contents by 48.8–55 % and 42.4–47.2 %, respectively with limited effects on the key flavor compound. Mechanistically, hydrocolloids formed protective matrices, scavenged free radicals, and reacted with Maillard reaction intermediates. These findings provide actionable insights for developing safer air-fried meat products without compromising quality, addressing critical public health concerns related to dietary carcinogens.
研究领域:
食品科学
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营养学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
SENSORS
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2020
·Vol. 20
·期 4
·1065
·
IF 3.275
Huihui Zhang,Wenqing Shao,Shanshan Qiu,Jun Wang,Zhenbo Wei
Aroma and taste are the most important attributes of alcoholic beverages. In the study, the self-developed electronic tongue (e-tongue) and electronic nose (e-nose) were used for evaluating the marked ages of rice wines. Six types of feature data sets (e-tongue data set, e-nose data set, direct-fusion data set, weighted-fusion data set, optimized direct-fusion data set, and optimized weighted-fusion data set) were used for identifying rice wines with different wine ages. Pearson coefficient analysis and variance inflation factor (VIF) analysis were used to optimize the fusion matrixes by removing the multicollinear information. Two types of discrimination methods (principal component analysis (PCA) and locality preserving projections (LPP)) were used for classifying rice wines, and LPP performed better than PCA in the discrimination work. The best result was obtained by LPP based on the weighted-fusion data set, and all the samples could be classified clearly in the LPP plot. Therefore, the weighted-fusion data were used as independent variables of partial least squares regression, extreme learning machine, and support vector machines (LIBSVM) for evaluating wine ages, respectively. All the methods performed well with good prediction results, and LIBSVM presented the best correlation coefficient (R2≥ 0.9998).Keywords:e-tongue;e-nose;rice wine;modified electrodes;smartphone
研究领域:
食品科学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2024
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IF 5.3
Remah Sobhy,Jianing Zhang,Zhihua Li,Xiaobo Zou,Asad Nawaz,Noman Walayat,Salim Manoharadas,Yangyang Jia,Mahmoud Salah
We aimed at tabulating and characterizing a flavan-3-ol isolated from date seeds into date seed pills (DSP) as a commercial healthcare pill, and deeply studying their anti-glycoxidation impacts on bovine serum albumin using multi-dimensional approaches SDS-PAGE/NBT, UPLC-ESI-Q-TOF-MS, antiradical via Fenton-type reaction, multispectral, and molecular docking. Results showed that DSP were successfully tabulated (an encapsulation efficiency of 98.78 %) with high antioxidant values via noncovalently binding with whey proteins supported by FTIR and lib-dock results. DSP dose-dependently decayed fluorescent-AGEs by 16.77, 5.32, and 37.41 % in DSP500-BSA-G/MGO/GO models, respectively, later confirmed by SDS-PAGE and initial precursors mitigation results. The docking results confirmed that DSP could bind with glycoxidation sites of BSA-subunits, namely Lys431, Arg458, and Arg196 via H-bonds. DSP trapped both di-carbonyls and reduced CML-level, where new adducts such as epicatechin-mono/MGO-GO, were identified. 500 µg mL −1 DSP reduced OH-induced fluorescence intensity of AGEs by 43.32 % in BSA-G/Fe 2+ /H 2 O 2 . Our results open a new direction for upcycling by-products as effective glycoxidation inhibitors in a type of healthcare pills in protein-rich models.
研究领域:
药学
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生物化学
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营养保健品
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食品科学
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产品关联
DOI
高影响因子
Frontiers in Nutrition
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2022
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IF 6.59
Feng Nianjie,Tang Fei,Hu Chuanqin,Cheng Lei,Lv Zhejuan,Shen Yang,Li Wei,Xiao Gengsheng,Dong Hao,Wu Qian
Lactic acid bacteria (LAB) have already been used as fermentation strains to enhance the antioxidant capacity of polyphenols. Antioxidant capacity is one of the most important factors to inhibit advanced glycation end product (AGE) formation and could LAB increase the inhibitory capacity of procyanidins on AGEs formation? It was surprising that opposite results were obtained both in simulated food processing and gastrointestinal digestion systems. After incubation with Lactobacillus plantarum (L. plantarum), litchi pericarp oligomeric procyanidins (LPOPCs) were bioconverted to several phenolic acids, which increased the antioxidant activity as expected. However, antiglycation ability and trapping carbonyl compounds capacity both weakened and it might be the primary reason for decreasing the inhibitory effect on AGE formation. Furthermore, it was found that LPOPCs incubated with L. plantarum inhibited the activity of digestive enzymes and thus decreased the digestibility of glycated protein. Our study systematically proposed for the first time that procyanidins bioconversion is an effective means to improve the antioxidant activity but has no remarkable promoting effect on AGEs inhibition.
研究领域:
食品科学
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生物化学
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医学
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产品关联
DOI
PubMed
高影响因子
Food Science and Human Wellness
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2024
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IF 5.6
Qiaozhi Zhang,Huatao Li,Ruixing Zheng,Lili Cao,Shufen Zhang,Shuifeng Zhang,Huadong Sheng,Yuhao Jiang,Yanbo Wang,Linglin Fu
Advanced glycation end-products (AGEs) are a group of heterogeneous compounds formed in heat-processed foods and are proven to be detrimental to human health. Currently, there is no comprehensive database for AGEs in foods that covers the entire range of food categories, which limits the accurate risk assessment of dietary AGEs in human diseases. In this study, we first established an isotope dilution UHPLC-QqQ-MS/MS-based method for simultaneous quantification of 10 major AGEs in foods. The contents of these AGEs were detected in 334 foods covering all main groups consumed in Western and Chinese populations. Nε -Carboxymethyllysine, methylglyoxal-derived hydroimidazolone isomers, and glyoxal-derived hydroimidazolone-1 are predominant AGEs found in most foodstuffs. Total amounts of AGEs were high in processed nuts, bakery products, and certain types of cereals and meats (> 150 mg/kg), while low in dairy products, vegetables, fruits, and beverages (< 40 mg/kg). Assessment of estimated daily intake implied that the contribution of food groups to daily AGE intake varied a lot under different eating patterns, and selection of high-AGE foods leads to up to a 2.7-fold higher intake of AGEs through daily meals. The presented AGE database allows accurate assessment of dietary exposure to these glycotoxins to explore their physiological impacts on human health.
研究领域:
营养学
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食品安全
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生物化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
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IF 3.571
Wei Wei,Jie Yang,Dan Yang,Xiangyu Wang,Zhenyu Yang,Qingzhe Jin,Manyi Wang,Jianqiang Lai,Xingguo Wang
We compared phospholipids (PLs), PL fatty acid (FA) composition, and milk fat globule size and structure in human milk (n = 120) from mothers of full-term and preterm infants during lactation (colostrum, transition, 1 mo, 2 mo, and 3 mo) and 8 brands of infant formulas. The absolute quantification of PLs was analyzed using 31P NMR spectroscopy. Sphingomyelin was the dominant PLs (35.01 ± 3.31%) in human milk, whereas phosphatidylcholine and phosphatidylethanolamine were the dominant PLs in infant formulas. The PL content in preterm milk increased during lactation, whereas that in term milk remained stable. Saturated FAs (mainly 16:0 and 18:0) were the most abundant (>60%) PL FA in both preterm and term milk and increased throughout lactation. The mean diameter of milk fat globules in infant formulas was much smaller than that found in human milk (200 nm vs 5.63 μm). Significant differences were observed between human milk and infant formulas with regard to PLs, suggesting that more research is needed to mimic the PL profile in infant formulas.
研究领域:
营养学
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生物化学
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DOI
PubMed
高影响因子
Bioengineered
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2021
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IF 3.269
Yunxin Zhang,Jianlong Liu,Wei Jia,Xuan Tian,Peng Jiang,Zhiyuan Cheng,Jinyong Li
This study is aimed at identifying the roles of AGE/RAGE and ET-1 in deep vein thrombosis (DVT). Advanced glycation end products (AGEs) in glycated human serum albumin (M-HSA) were detected by ELISA. The viability of HUVECs was examined by CCK-8 assay. Flow cytometry was performed to detect cell apoptosis, followed by ELISA for the detection of inflammatory cytokine level and oxidative stress level in HUVECs. Immunofluorescence was performed to detect ET-1 and eNOS expression. The expression of specific proteins was assayed by western blot. As a result, decreased HUVEC viability was observed after stimulation with M-HSA, whereas RAGE inhibitor improved it. Cell apoptosis showed the opposite trend. Additionally, M-HSA-induced inflammatory cytokine release and oxidative stress of HUVECs were both alleviated by RAGE inhibitor. RAGE inhibitor also increased the levels of NO and eNOS while decreasing the level of ET-1 in M-HSA-stimulated HUVECs. Furthermore, decreased protein expression of Bax, cleaved-caspase3, RAGE, p65, ET-1 and iNOS was observed after treatment with RAGE inhibitor, in addition to increased protein expression of Bcl-2 and eNOS. In conclusion, blocking AGE/RAGE pathway downregulates ET-1, thereby mitigating HUVEC damage in DVT.
研究领域:
心血管疾病
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分子生物学
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细胞生物学
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血栓形成
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Yuhang Xin,Yingxi Dong,Jiasheng Zhang,Jiajing Liu,Zhuoer Tian,Xiang Duan,Xuebo Liu
This study investigated the inhibition of lactoferrin (LF) glycation by three pineapple peel-derived polyphenols—gallic acid (GA), epicatechin (EC), and ferulic acid (FA). Spectroscopic analysis and molecular docking revealed that each polyphenol binds to LF via hydrogen bonds through static quenching, increasing α-helix content by 1.38%–2.48% and enhancing structural stability. LF-polyphenol complexes exhibited significantly improved DPPH (77.93%–88.74%) and ABTS (55.25%–68.05%) radical scavenging rates compared to LF alone. In vitro anti-glycation assays demonstrated that all three polyphenols effectively inhibited AGEs formation, surpassing aminoguanidine (1 mM) at 12 days. Mechanistic studies indicate these polyphenols inhibit AGEs through multiple combined pathways: potent antioxidant activity, metal ion chelation (up to 46.07%), effective α-dicarbonyl capture, and stabilization of LF structure.
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DOI
PubMed
高影响因子
PHYTOMEDICINE
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2020
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IF 4.268
Nani Wang,Pingcui Xu,Xuping Wang,Weixuan Yao,Binjie Wang,Yuanzhao Wu,Dan Shou
Background Advanced glycation end products (AGEs) deposition causes inflammatory injury in osteoblasts and contributes to diabetic osteoporosis. The receptor for advanced glycation end product/mitogen-activated protein kinase pathway (RAGE/MAPK) signaling pathway is closely linked to the pathogenesis of diabetic osteoporosis. Timosaponin AIII, a steroidal saponin isolated from Anemarrhena asphodeloides Bunge (Asparagaceae), shows anti-inflammatory and anti-osteoporosis effects. Purpose The present study was aimed to investigate the therapeutic effects of timosaponin AIII on diabetic osteoporosis and whether its effect is dependent on protecting osteoblasts against AGEs-induced injury via RAGE/MAPK signaling suppression. Methods An alloxan-induced diabetic osteoporosis zebrafish model was applied to investigate the effects of timosaponin AIII in vivo , and alendronate was used as a positive control. Moreover, related mechanisms were explored in primary rat osteoblasts. Molecular docking was applied to investigate the interactions between timosaponin AIII and RAGE. Results Timosaponin AIII treatment reversed alloxan-induced reduction in the mineralized area of the larvae head skeleton, accompanied by a decreased level of triglyceride and total cholesterol in the zebrafish. Additionally, AGEs significantly influenced RAGE expression, alkaline phosphatase activity, interleukin 1β expression, interleukin 6 expression, and tumor necrosis factor-α expression, and increased cell apoptosis. Timosaponin AIII significantly downregulated AGEs-induced interleukin 1β, interleukin 6, and tumor necrosis factor-α levels, and upregulated alkaline phosphatase and osteocalcin levels. Timosaponin AIII also significantly reduced the expression of RAGE and had additive effects on downstream P38, extracellular signal-regulated kinase and c-Jun N-terminal kinase in AGEs-induced osteoblast. Molecular docking predicted that hydrogen and hydrophobic interactions occurred between timosaponin AIII and RAGE. Conclusion These data clarified that timosaponin AIII attenuates diabetic osteoporosis via a novel mechanism involved suppressing the RAGE/MAPK signaling pathway. Our finding highlights the potential value of timosaponin AIII as an anti-diabetic osteoporosis agent.
研究领域:
药理学
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生物学
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医学
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内分泌学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 5
·657
·
IF 5.561
Huiyu Hu,Yuting Wang,Yousheng Huang,Yanpeng Yu,Mingyue Shen,Chang Li,Shaoping Nie,Mingyong Xie
Maillard reaction during food processing contributes to the formation of some unpleasant heat-induced toxicants including advanced glycation end products (AGEs) and 5-hydroxymethylfurfural (HMF). The current study prepared butter cookies fortified with two dietary natural antioxidants (catechins and curcumin) and two dietary hydrocolloids (pectin and chitosan), and investigated their effects on formation of free Nε-(carboxymethyl)lysine (CML)/Nε-(carboxyethyl)lysine (CEL), protein-bound CML/CEL and HMF and on the sensory qualities of butter cookies. Meanwhile, three typical α-dicarbonyl compounds were also determined to identify possible correlations between α-dicarbonyl intermediates and formation of these harmful heat-induced products in butter cookies. Experimental data showed that catechin exhibited the strongest inhibitory effects on formation of AGEs and HMF, but its addition would impair the color and taste of cookies. On the other hand, chitosan was not so effective in inhibiting AGEs and HMF as compared to catechin, but its addition could increase the sensory qualities of butter cookies.Keywords:advanced glycation end products;5-hydroxymethylfurfural;butter cookies;natural antioxidants;hydrocolloids
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 2
·1667
·
IF 5.6
Shengqi Huo,Qian Wang,Wei Shi,Lulu Peng,Yue Jiang,Mengying Zhu,Junyi Guo,Dewei Peng,Moran Wang,Lintong Men,Bingyu Huang,Jiagao Lv,Li Lin
Cuproptosis resulting from copper (Cu) overload has not yet been investigated in diabetic cardiomyopathy (DCM). Advanced glycosylation end products (AGEs) induced by persistent hyperglycemia play an essential role in cardiotoxicity. To clarify whether cuproptosis was involved in AGEs-induced cardiotoxicity, we analyzed the toxicity of AGEs and copper in AC16 cardiomyocytes and in STZ-induced or db/db-diabetic mouse models. The results showed that copper ionophore elesclomol induced cuproptosis in cardiomyocytes. It was only rescued by copper chelator tetrathiomolybdate rather than by other cell death inhibitors. Intriguingly, AGEs triggered cardiomyocyte death and aggravated it when incubated with CuCl2or elesclomol–CuCl2. Moreover, AGEs increased intracellular copper accumulation and exhibited features of cuproptosis, including loss of Fe–S cluster proteins (FDX1, LIAS, NDUFS8 and ACO2) and decreased lipoylation of DLAT and DLST. These effects were accompanied by decreased mitochondrial oxidative respiration, including downregulated mitochondrial respiratory chain complex, decreased ATP production and suppressed mitochondrial complex I and III activity. Additionally, AGEs promoted the upregulation of copper importer SLC31A1. We predicted that ATF3 and/or SPI1 might be transcriptional factors of SLC31A1 by online databases and validated that by ATF3/SPI1 overexpression. In diabetic mice, copper and AGEs increases in the blood and heart were observed and accompanied by cardiac dysfunction. The protein and mRNA profile changes in diabetic hearts were consistent with cuproptosis. Our findings showed, for the first time, that excessive AGEs and copper in diabetes upregulated ATF3/SPI1/SLC31A1 signaling, thereby disturbing copper homeostasis and promoting cuproptosis. Collectively, the novel mechanism might be an alternative potential therapeutic target for DCM.Keywords:cuproptosis;advanced glycosylation end products;diabetic cardiomyopathy;SLC31A1
研究领域:
心脏病学
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糖尿病
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分子生物学
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细胞信号传导
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病理生理学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2021
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IF 4.24
Chuan Qin,Yueqin Li,Yunzhen Zhang,Lianliang Liu,Zufang Wu,Peifang Weng
In this study, the inhibitory effects of oat polyphenols constituent on advanced glycation end products (AGEs) formation in the bovine serum albumin (BSA)-glucose and BSA-fructose models were explored. Spectroscopic techniques , SDS-PAGE and molecular docking were used to characterize the antiglycation capacity of oat polyphenols constituent. The results unveiled that apigenin and luteolin had the strongest inhibitory effect on AGEs. Spectroscopy results indicated that apigenin and luteolin inhibited the BSA glycation in a dose-dependent manner by attenuating the changes of conformational structure and microenvironment induced by glycation. Apigenin and luteolin also suppressed the cross-linking or aggregation of glycated BSA, which was reflected in the changed molecular weight and determined by SDS-PAGE. The prediction of molecular docking demonstrated that binding affinity of BSA-luteolin was greater than that of apigenin, which might be due to the additional hydroxyl group in the position of A ring of luteolin. In conclusion, luteolin had a stronger AGEs inhibitory activity than apigenin ( P < 0.05). Oat polyphenols may be a good food source for inhibiting the formation of AGEs in vitro .
研究领域:
食品科学
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生物化学
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分子生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）