MICROCHIMICA ACTA
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2023
·Vol. 190
·期 9
·1-11
·
IF 5.7
Liu Yujie,Gao Ran,Liu Xingcen,Zheng Jinhua,Wu Xia
Curcumin (Cur) possesses diverse biological and pharmacologic effects. It is widely used as a food additive and therapeutic medicine. A study to determine a sensitive detection method for Cur is necessary and meaningful. In this work, double rare earth ions co-doped fluorescent coordination polymer nanoparticles (CPNPs) were developed for the Cur detection. The CPNPs were synthesized by using adenosine monophosphate (AMP) as bridge ligands via coordination self-assembly with Ce 3+ and Tb 3+ . The AMP-Ce/Tb CPNPs exhibited the characteristic green fluorescence of Tb 3+ and had high luminescence efficiency. Under the optimal conditions, the fluorescence intensity of AMP-Ce/Tb CPNPs could be significantly quenched by Cur. The fluorescence quenching extent at λ ex / λ em of 300 nm/544 nm showed a good linear relationship with the Cur concentration in the range of 10 to 1000 nM. The detection limit was as low as 8.0 nM ( S/N = 3). This method was successfully applied to the determination of Cur in real samples with satisfactory results. The luminescence mechanism of AMP-Ce/Tb CPNPs and the fluorescence quenching mechanism of the CPNPs by Cur were both examined. Graphical abstract
研究领域:
分析化学
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生物医学
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材料科学
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PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Chunling Nie,Zejie Chen,Yijun Liu,Zhonghui Shen,Yujia Dang,Yingtian Qu,Pengrui Wu,Hong Zeng,Yanbo Wang,Jianguo Wang
Salt ions induced CNCs aggregation by electric double layer compression. • Aggregated CNCs formed interfacial bridging structures between emulsion droplets. • Salt-induced CNCs networks enhanced interfacial reinforcement and emulsion viscosity. • AMP/HPMC Pickering emulsions exhibited remarkable long-term stability at 60 days. • Salt-induced CNCs aggregation hindered the contact between AMP and digestive enzymes. Salt ions play a key role in governing the property of food system. In this work, the effects of salt ions on physicochemical property of cellulose nanocrystals (CNCs) and their stabilization behavior in Amylopectin/Hydroxypropyl methylcellulose (AMP/HPMC) water-in-water (W/W) emulsions were investigated. The addition of NaCl significantly reduced the absolute value of zeta potential of CNCs and resulted in particle aggregation, forming of network structures. These structure changes promoted the droplet clustering and bridging flocculation of AMP/HPMC W/W Pickering emulsions and enhanced their stability during long-term storage (60 days). Rheological analysis revealed that increasing NaCl concentration resulted in enhanced viscosity and obvious viscous-dominated behavior due to the synergistic interaction between salt-induced CNCs network and thermal-induced aggregation of HPMC. Furthermore, in vitro digestion results showed that salt-induced structural changes of CNCs effectively limited enzyme accessibility, thereby reducing AMP digestion and glucose release. This work demonstrates that salt ions could regulate the structure-function relationship of CNCs-stabilized W/W Pickering emulsions, and provides new insights to design novel food systems with controlled digestion properties. Download: Download high-res image (188KB) Download: Download full-size image
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CHEMICAL ENGINEERING SCIENCE
·
2026
·
IF 5.1
Xiaoxia Ye,Feiyang Wang,Juan Liu,Yifan Liu,Yuancai Lv,Chunxiang Lin,Liang Song,Minghua Liu,Ying Zhang,Jie Chen
Radioactive 137 Cs poses a severe threat in nuclear wastewater. Conventional powdered adsorbents suffer from poor recoverability and are ill-suited for large-scale continuous-flow operations. To address these limitations, a novel three-dimensional bulk ammonium phosphomolybdate-gelatin composite adsorbent (AMP@GA) was fabricated. Through this strategy, highly selective AMP particles were uniformly immobilized within a porous (92%) gelatin network. Rapid adsorption kinetics (reaching equilibrium within 30 min), a Langmuir-fitted theoretical maximum Cs + adsorption capacity of 142.17 mg·g −1 at 45 °C, and acceptable chemical stability across a broad pH range (2.0-11.0) were exhibited by the resulting AMP@GA. Most significantly, the material demonstrated outstanding selectivity over competing ions ( K d = 7.48 × 10 4 mL·g −1 ). Its practical viability was confirmed by achieving over 88% removal efficiency in authentic seawater. Furthermore, in fixed-bed column tests, the material sustained continuous operation for 163.5 h. The mechanism is dominated by NH 4 + -Cs + ion exchange, assisted by electrostatic interactions. This work presents a scalable, easily recoverable bulk adsorbent that effectively helps address the engineering bottlenecks of traditional powdered materials.
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Microbial Biotechnology
·
2024
·Vol. 17
·期 4
·e14455
·
IF 4.8
Xuke Yang,Jichao Yang,Mengyu Lyu,Yaqiong Li,Anqi Liu,Bang Shen
Toxoplasma gondii is a zoonotic parasite infecting humans and nearly all warm-blooded animals. Successful parasitism in diverse hosts at various developmental stages requires the parasites to fine tune their metabolism according to environmental cues and the parasite's needs. By manipulating the β and γ subunits, we have previously shown that AMP-activated protein kinase (AMPK) has critical roles in regulating the metabolic and developmental programmes. However, the biological functions of the α catalytic subunit have not been established. T. gondii encodes a canonical AMPKα, as well as a KIN kinase whose kinase domain has high sequence similarities to those of classic AMPKα proteins. Here, we found that Tg KIN is dispensable for tachyzoite growth, whereas Tg AMPKα is essential. Depletion of Tg AMPKα expression resulted in decreased ATP levels and reduced metabolic flux in glycolysis and the tricarboxylic acid cycle, confirming that Tg AMPK is involved in metabolic regulation and energy homeostasis in the parasite. Sequential truncations at the C-terminus found an α-helix that is key for the function of Tg AMPKα. The amino acid sequences of this α-helix are not conserved among various AMPKα proteins, likely because it is involved in interactions with Tg AMPKβ, which only have limited sequence similarities to AMPKβ in other eukaryotes. The essential role of the less conserved C-terminus of Tg AMPKα provides opportunities for parasite specific drug designs targeting Tg AMPKα.
研究领域:
寄生虫学
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分子生物学
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医学
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PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Wu Jiequn,Chen Danqing,Wu Jinrong,Chu Xiaohe,Yang Yongmei,Fang Lina,Zhang Wei
Daptomycin, which is produced by Streptomyces roseosporus, has been characterized as a novel cyclic lipopeptide antibiotic that is effective against Gram-positive bacteria. The biosynthesis of daptomycin is regulated by various factors. In the present study, we demonstrated that the cyclic AMP receptor protein (Crp) plays an important role in producing daptomycin in the S. roseosporus industrial strain. We found that daptomycin production from the crp deletion strain decreased drastically, whereas production from the crp overexpression strain increased by 22.1%. Transcriptome and qPCR analyses showed that some genes related to the daptomycin biosynthetic gene cluster (dpt) and the pleiotropic regulator (adpA) were significantly upregulated. RNA-seq also shows Crp to be a multifunctional regulator that modulates primary metabolism and enhances precursor flux to secondary metabolite biosynthesis. These results provide guidance for the development and improvement of potential natural products.
研究领域:
微生物学
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分子生物学
·
药学
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PubMed
高影响因子
Joule
·
2026
·
IF 37.1
Shu Yuan,Lizhen Wu,Rongyi Wang,Rui Xue,Fangyuan Li,Huiyuan Li,Guiru Zhang,Peng Shen,Zhiming Bao,Congfan Zhao,Qing Wang,Jiewei Yin,Liuxuan Luo,Shuiyun Shen,Liang An,Xiaohui Yan,Junliang Zhang
Industrial implementation of electrochemical CO 2 reduction requires maximizing both throughput and durability. While conventional rib-channel flow fields are the current standard, further elevating their performance is constrained by the inherent trade-offs involving CO 2 transport, water management, and salt precipitation behavior. To overcome these coupled challenges, we introduce a 3D foam flow field architecture. This design utilizes an interconnected porous structure to provide moderate convective transport, effectively mitigating local CO 2 starvation and enabling effective passive water management. Consequently, we achieve a CO Faradaic efficiency of 90% at an industrially relevant current density of 1,000 mA cm −2 . The optimized transport yields a high CO product concentration of 74 vol % and increases single-pass conversion to 43%. The device also demonstrates superior stability with a degradation rate only 1/14 of the baseline design, maintaining performance for over 400 h. This highlights that advanced flow field engineering is essential for unlocking industrial-scale CO 2 electrolysis.
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npj Science of Food
·
2025
·
IF 7.8
Cao Liguang,Li Guojin,Liu Kai,Gao Wenrui,Dong Yifei,Yang Zifeng,Luo Jianchun,Lei Lin,Du Xiliang,Song Yuxiang,Li Xinwei,Gao Wenwen,Liu Guowen
Deoxynivalenol (DON) and fumonisin B1 (FB1) are prevalent mycotoxins commonly found together in dairy cow feed, posing significant risks to cow health and food safety. This study investigates combined toxic effects of DON and FB1 on bovine mammary epithelial cells (BMECs), and role of AMPK-Caspase 6 axis in mycotoxin-induced apoptosis in BMECs and mouse model. We showed that even subcytotoxic doses of DON, combined with FB1, induced synergistic or additive toxic effects, reducing cell viability, increasing LDH release, and promoting apoptosis. This combination also impaired milk triglyceride synthesis and downregulated genes involved in milk fat and protein production. Silencing Caspase 6 via siRNA, inhibiting with Z-VEID-FMK, or activating AMPK with AICAR alleviated apoptosis. Conversely, overexpressing Caspase 6 diminished AMPK’s protective effects. In vivo, inhibiting Caspase 6 alleviated apoptosis in mammary tissues. These findings highlight therapeutic potential of targeting the AMPK-Caspase 6 pathway to mitigate combined toxicity of DON and FB1.
研究领域:
毒理学
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分子生物学
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兽医学
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食品安全
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细胞生物学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shaoyang Zhi,Junli Wang,Yiran Wang,Yijie Li,Mengjuan Zhao,Liping Yang,Chaobin Qin,Xiao Yan,Guoxing Nie
AMPKα1 and AMPKα2, key kinases in regulating energy homeostasis, have not been previously cloned or characterized in common carp ( Cyprinus carpio ). This study identified the open reading frame (ORF) sequences of ampkα1 (1722 bp, encoding 573 amino acids) and ampkα2 (1659 bp, encoding 552 amino acids) through homologous cloning. Sequence alignment and phylogenetic analysis showed a high similarity of both genes with fish homologs. Expression analysis revealed that ampkα1 and ampkα2 are widely expressed across tissues in carp, with ampkα1 highly expressed in the gonads and ampkα2 in the heart. Fasting significantly reduced ampkα1 expression in the heart, adipose tissue, and foregut but increased it in the hindgut and white muscle. Similarly, ampkα2 expression decreased in the hypothalamus and muscle during fasting, with an increase in the midgut. Glucose tolerance tests showed dynamic regulation of ampkα1 and ampkα2 , with initial downregulation followed by upregulation in the hepatopancreas, red muscle, and brain. High-glucose and high-fat diets significantly increased ampkα1 and ampkα2 expression in multiple tissues. Insulin and glucagon treatment induced time-dependent changes in both genes in hepatocytes, while Aeromonas hydrophila infection, LPS, and Poly (I:C) stimulation upregulated ampkα1 and ampkα2 in immune-related tissues. Knockdown of ampkα2 , but not ampkα1 , reduced glut1b mRNA levels, while both knockdowns of ampkα1 and ampkα2 promoted the expression of gsk3β , pygm , acc , fas , srebp , cs , and pro-inflammatory cytokines, suggesting their involvement in metabolic and immune regulation in carp.
研究领域:
分子生物学
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营养学
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免疫学
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PubMed
高影响因子
MEDICAL SCIENCE MONITOR
·
2018
·
IF 1.894
Xi Chen,Kai Li,Guoqing Zhao
Background Propofol has antitumor effects against various cancers. However, the mechanism of action of propofol in HeLa human cervical cancer cells has not been elucidated. Material/Methods We treated HeLa human cervical cancer cells with different concentrations of propofol. Cell viability was evaluated with Cell Counting Kit-8 and apoptosis was analyzed by annexin V-fluorescein isothiocyanate and propidium iodide staining and flow cytometry. Autophagosome formation was evaluated based on microtubule-associated protein light chain (LC)3 conversion and light chain 3 puncta formation. Autophagosome clearance was assessed according to p62 protein level and autolysosome generation. Results We found that propofol decreased cell viability and increased autophagosome generation in HeLa cells. Autophagosome formation was evaluated based on LC3 conversion and LC3 puncta formation. Autophagosome clearance was assessed according to p62 protein level. The AMPK/mTOR signaling pathway was found to be activated in propofol-induced autophagosome accumulation. Fluorescence analysis using LysoTracker dye revealed that propofol blocked autophagosome–lysosome fusion. Administration of rapamycin increased autophagosome clearance in propofol-treated HeLa cells. Additionally, propofol induced endoplasmic reticulum (ER) stress and disrupted intracellular Ca 2+ balance, thereby enhancing autophagosome accumulation. Suppressing ER stress by treatment with tauroursodeoxycholic acid (TUDCA) enhanced these effects, suggesting that the cytotoxicity of propofol is related to induction of ER stress. Conclusions This study is the first to provide evidence that propofol-mediated autophagy regulation is an underlying part of the mechanism by which propofol regulates HeLa cells progression.
研究领域:
肿瘤学
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细胞生物学
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药理学
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PubMed
高影响因子
Theranostics
·
2024
·Vol. 14
·期 17
·6571
·
IF 12.4
Xiaodie Li,Minglong Chen,Xinni He,Jinghang Cong,Wanchen Zhao,Yanping Fu,Chao Lu,Chuanbin Wu,Xin Pan,Guilan Quan
The endogenous immunomodulator adenosine (ADO) was expected to be potentialized as an efficacious mediator to combat psoriasis. However, its efficacy is severely hindered by its poor metabolic stability and insufficient accumulation at the dermatological lesions. Methods: In this study, a biomineralized in situ catalytic nanoreactor was delicately customized by encapsulating ADO precursor (adenosine monophosphate, AMP) within the internal porous skeleton of zeolitic imidazolate framework-90, followed by the biomineralization of the AMP catabolic enzyme on the outer layer. The nanocrystals were then incorporated into a dissolving microneedles patch, which was designed to deliver drugs with precision into the cutaneous lesion and enhance the efficacy of psoriasis treatment. Results: Upon penetration into the skin, the nanoreactors were released and underwent a gradual collapse of their structure, releasing AMP when exposed to the acidic microenvironment. Meanwhile, the acidic pH could trigger an in situ catalytic reaction to continuously produce ADO. This system yielded remarkable results in a psoriasis-like mouse model. The mechanism study demonstrated that this system could substantially reshape the inflammatory ecosystem by inhibiting the keratinocyte hyperplasia, reducing inflammatory cytokine expression, and regulating the infiltration of immune cells. Conclusion: The in situ catalytic nanoreactor integrated microneedle patch is a promising modular platform for co-delivery of prodrugs and their catabolic enzymes, offering a potential solution for various diseases.
研究领域:
药学
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纳米技术
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皮肤病学
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免疫学
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PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Weiliang Guan,Wenqian Nong,Xiaobo Wei,Renchi Chen,Zhihai Huang,Yuting Ding,Xiaoming Qin,Luyun Cai,Linchun Mao
Background Water-free transportation (WFT), as a novel strategy for express delivery of live shrimp ( Litopenaeus vannamei ), was recently developed. However, air exposure during this transportation arouses a series of abiotic stress to the shrimp. In this work, the influences of WFT stress on glycolysis and lipolysis metabolism and meat quality (umami flavor and drip loss) were investigated in comparison with the conventional water transportation (WT). Results Results showed that type II muscle fibers with the feature of anaerobic metabolism were dominated in shrimp flesh. In addition, the increments of intracellular Ca 2+ was detected in WFT and WT, which then activated the AMP-activated protein kinase (AMPK) pathway, and thereby promoted the consumption of glycogen, accumulation of lactate and lipolysis under the enzymolysis of hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), and adipose triglyceride lipase (ATGL). Glycogen glycolyzed to latate. Meanwhile, ATP degraded along with glycolysis resulting in the generation of ATP-related adenosine phosphates such as inosine monophosphate (IMP) with umami flavor and phosphoric acid. More remarkable ( p < 0.05) physiological changes (except LDH and lactate) were observed in WFT compared to WT. Additionally, fatty acid profile also slightly changed. Conclusions The transport stress induced significant energy metabolism changes of shrimp flesh, and therefore effected the flesh quality. The intensifications of freshness (K value) of shrimp flesh were detected due to ATP degradation, which were more pronounced after WFT. However, the drip loss of shrimp flesh was more significantly increased ( p < 0.05) after WFT compared to WT. This article is protected by copyright. All rights reserved.
研究领域:
水产养殖
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生物化学
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代谢
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食品科学
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PubMed
高影响因子
CELLULOSE
·
2023
·Vol. 30
·期 10
·6695-6718
·
IF 5.7
Zhang Xuefeng,Li Dan,You Fei,Li Gang,Zhou Chang,Cheng Dan,Pan Yu,Wang Junqi,Ma Jing
Cotton fabrics were treated with Gelatin (GEL)/Adenosine 5′-monophosphate (AMP) by layer-by-layer (LBL) assembly coatings and SiO 2 sol–gel coatings to improve flame retardancy. Functional groups, surface element compositions, crystal structures, surface microscopic morphologies and element distributions of original and treated cotton fabrics were characterized by techniques including Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and Scanning electron microscopy–Energy dispersive spectrometer (SEM–EDX). The results of FTIR, XPS, XRD and SEM–EDX show that the composite coatings are successfully deposited onto cotton fiber surfaces. Thermogravimetric, limiting oxygen index (LOI) experiments, vertical flammability tests (VFTs) and cone calorimetry tests (CCTs) were used to assess the thermal stabilities, flame retardancy levels and reaction-to-fire properties of cotton fabrics. The washing durability, air permeability and hydrophobicity levels were investigated by American Association of Textile Chemists and Colorists, Chinese national standard and water contact angle tests. Results show that the 10BL-SiO 2 @COT exhibits optimum flame retardancy level. Its char residue rate at 700.0 ℃ increases by 506.7% when comparing with original cotton fabric. It has a LOI value of 30.3% (non-flammable level). It can self-extinguish with a char length of only 4.4 cm during a VFT. Its total heat release and peak heat release rate reduce by 18.7% and 50.4% when comparing with original cotton fabric. Its fire growth rate value decreases to 2.2 kW/(m 2 ·s) with a synergistic effectiveness parameter of 1.3 (≥ 1.0). In addition, its LOI value decreases by 26.1% after 10 laundering cycles, but its air permeability enhances by 5.8% as compared with original cotton fabric. Also, it is very hydrophilic. GEL/AMP LBL assembly and SiO 2 gel coatings play more synergistic roles in providing physical barriers and structural supports, promoting and reinforcing char formations, cooling burning zones, diluting oxygen and gaseous products.
研究领域:
纺织科学
·
材料科学
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高影响因子
危险品化学品经营许可证（带存储）