Advanced Science
·
2023
·Vol. 10
·期 11
·2207228
·
IF 15.1
Wei Zhang,Jing-Jing Hu,Rui Liu,Jun Dai,Lizhen Yuan,Yiheng Liu,Bochao Chen,Mingxing Gong,Fan Xia,Xiaoding Lou
Despite the promising advancements of in situ forming nanoassembly for the inhibition of tumor growth and metastasis, the lack of sufficient triggering sites and hardly controlling the forming position restrict their further developments. Herein, a smart transformable peptide-conjugated probe (DMFA) with enzyme cleavage-induced morphological change is designed for treatment on the tumor cell membrane. Specifically, after self-assembling into nanoparticles and anchoring on the cell membrane with sufficient interaction sites rapidly and stably, DMFA will be efficiently cleaved into α -helix forming part (DP) and β -sheet forming part (LFA) by overexpressed matrix metalloproteinase-2. Thus, the promoted Ca 2+ influx by DP-induced cell membrane breakage and decreased Na + /K + -ATPase activity by LFA-assembled nanofibers wrapping the cells can inhibit PI3K-Akt signaling pathway, leading to the inhibition of tumor cell growth and metastasis. This peptide-conjugated probe undergoes in situ morphological transformation on the cell membrane, exhibiting great potential in tumor therapy.
研究领域:
生物技术
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纳米技术
·
癌症治疗
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Wang Yang-Yang,Sun Yan-Ping,Yang Bing-You,Wang Qiu-Hong,Kuang Hai-Xue
Herbs with a “hot” properties are frequently used to treat cold symptoms in TCM. However, the underlying mechanisms of the herbs with “hot” properties on hypothyroidism have not been investigated. This study aimed to explore four typical “hot” and “cold” property herb on hypothyroidism. Firstly, the difference efficacy between the four typical “hot” property herbs and the four typical “cold” property herbs was assessed by physical signs, thyroid function, and the metabolic profile using multivariate statistical analysis. The influence of the four typical “hot” property herbs on hypothyroidism was validated pathologically. The impact mechanism of the four typical “hot” property herbs on hypothyroidism was investigated through a metabolomics method combined with network analysis. Na+/K+-ATP, ACC1 enzyme, UCP-1, and the PI3K-Akt pathway were used to confirm the metabolite pathways and target-associated metabolites. The results showed that the four typical “hot” property herbs could significantly improve physical signs, thyroid function, and the metabolic profile in hypothyroidism rats, the four typical “cold” property herbs did not show any benefit. Moreover, the four typical “hot” property herbs could improve lipid metabolism, energy metabolism, and thyroid hormone levels by the PI3K-Akt signaling pathway, Ca2+- AMPK signaling pathways, purine metabolism, and tryptophan metabolism. Additionally, the levels of UCP-1, Na+/K + -ATP enzyme, and ACC1 were ameliorated by the four typical “hot” property herbs in hypothyroidism rats. Therefore, a metabolomics strategy combined with network analysis was successfully performed and interpreted the mechanism of the four typical “hot” property herbs on hypothyroidism based on the theory of “cold and hot” properties of TCM well.
研究领域:
中医中药
·
药理学
·
代谢组学
·
内分泌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2016
·Vol. 11
·期 4
·e0151479
·
IF 3.057
Huiqing Wu,Qingping Wu,Guojie Wu,Qihui Gu,Linting Wei
The goal of this study was to identify Cd-resistant bacterial strains with endurance capacity and to evaluate their ability to remove cadmium ions from cadmium-polluted water. The Bacillus cereusS5 strain identified in this study had the closest genetic relationship with B . cereus sp. Cp1 and performed well in the removal of Cd 2+ ions from solution. The results showed that both the live and dead biomasses of the Cd 2+ -tolerant B . cereus S5 strain could absorb Cd 2+ ions in solution but that the live biomass of the B . cereus S5 strain outperformed the dead biomass at lower Cd 2+ concentrations. An analysis of the cadmium tolerance genes of B . cereus S5 identified ATPase genes that were associated with cadmium tolerance and involved in the ATP pumping mechanism. The FTIR spectra revealed the presence of amino, carboxyl and hydroxyl groups on the pristine biomass and indicated that the cadmium ion removal ability was related to the structure of the strain. The maximum absorption capacity of the B . cereus S5 strain in viable spore biomass was 70.16 mg/g (dry weight) based on a pseudo-second-order kinetic model fit to the experimental data. The Langmuir and Langmuir-Freundlich isotherm adsorption models fit the cadmium ion adsorption data well, and the kinetic curves indicated that the adsorption rate was second-order. For Cd 2+ concentrations (mg/L) of 1–109 mg/L, good removal efficiency (>80%) was achieved using approximately 3.48–10.3 g/L of active spore biomass of the B . cereus S5 strain. A cadmium-tolerant bacteria-activated carbon-immobilized column could be used for a longer duration and exhibited greater treatment efficacy than the control column in the treatment of cadmium-polluted water. In addition, a toxicity assessment using mice demonstrated that the biomass of the B . cereus S5 strain and its fermentation products were non-toxic. Thus, the isolated B . cereus S5 strain can be considered an alternative biological adsorbent for use in emergency responses to severe cadmium pollution and in the routine treatment of trace cadmium pollution.
研究领域:
环境微生物学
·
生物修复
·
水处理
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2023
·
IF 7
Xiuling Meng,Weijie Wu,Ben Niu,Ruiling Liu,Huizhi Chen,Haiyan Gao,Hangjun Chen
Perillaldehyde , the predominant antimicrobial compound found in Perilla , offers a safe and highly efficient solution for the preservation of fruits and vegetables. This study delved into the inhibitory potential of perillaldehyde against Fusarium graminearum , the primary postharvest pathogen affecting ginger. The current research encompassed both in vitro and in vivo investigations. Our results revealed that the minimum inhibitory concentration of perillaldehyde required to combat F. graminearum was 240 µL·L −1 . At this concentration, perillaldehyde exhibited remarkable efficacy in reducing ginger disease rates, with a staggering 99.9% inhibition in mycelium growth. Furthermore, perillaldehyde disrupted the integrity of the fungal cell membrane, hence elevated outer membrane permeability . This resulted in the leakage of proteins, sugars, nucleic acids , and an increase in electrical conductivity within F. graminearum . Notably, perillaldehyde treatment led to a substantial increase in malondialdehyde content in F. graminearum , which was 2.5 times higher than the control group. It also significantly reduced ergosterol content and ATPase activity by 87% and 85%, respectively. The presence of reactive oxygen species (ROS) increased, exacerbating membrane peroxidation damage. Electron microscopy unveiled the transformative impact of perillaldehyde on F. graminearum . Perillaldehyde induced extensive shrinkage, morphological thinning, spatial structure folding, and the leakage of essential cell nutrients, cell wall membranes, and organelle damage. In summary, perillaldehyde's mode of action disrupts the energy supply and metabolism of mycelia, ultimately inducing apoptosis and proving to be a potent inhibitor of F. graminearum . Its application in preserving fresh ginger effectively extended the nutritional quality of the product, demonstrating its potential as an efficient preservative. This biological preservation technique offers convenience over traditional physical methods and addresses concerns related to chemical residue, thereby opening new avenues for ginger green preservation technology.
研究领域:
植物病理学
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食品科学
·
微生物学
·
农业化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Yongsheng Ni,Mengyi Wang,Lizhi Liu,Mile Li,Shuhui Hu,Junwei Lin,Jing Sun,Tianli Yue,Ming-Qiang Zhu,Jianlong Wang
Photocatalytic disinfection is a promising way to combat bacterial pollution in the water environment. Inefficient use of visible light and undirected diffusion of reactive oxygen species (ROS) reduce photocatalytic disinfection efficiency. Herein, inspired by the concentrating effect of convex lens, photocatalysts with particular “nano-magnifying glass effect” (TCNMgNOs) were designed by embedding magnesium oxide with “converge effect” into the tailored hierarchical triple-shell porous g-C 3 N 4 with “one light multi-purpose effect” to boost the visible-light utilization. Meanwhile, the ATPase hydrolysis homeostasis of bacteria was destroyed by TCNMgNOs to achieve the targeted movement of ROS. The results confirmed that the photocatalytic sterilization efficiency of TCNMgNOs was amplified by 30 times over g-C 3 N 4 , which was achieved by focusing visible light, multiple reflecting visible light and light transmission within the porous thin shells as well as the “addictive sterilization mechanism”. The sterilization efficiency still maintains 98.8 % (15 min) after 6 rounds recycling and reusing in practical river water disinfection . A novel pathway for fighting against microbial contaminants in natural water was explored.
研究领域:
环境科学
·
材料科学
·
化学
·
水处理技术
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Bohan Yuan,Jiachao Yao,Zeyu Wang,Luyao Dai,Min Zhao,Dzmitry Hrynsphan,Savitskaya Tatsiana,Jun Chen
In this work, Rhodococcus ruber HJM-8 and Paracoccus communis YBH-X were isolated and used to enhance N , N -dimethylacetamide (DMAC) degradation and mineralization efficiencies. The monoculture and co-culture of the two strains for DMAC degradation were compared; results indicated that, a degradation efficiency of 97.62% was obtained in co-culture, which was much higher than that of monocultures of HJM-8 (57.34%) and YBH-X (34.02%). The degradation mechanism showed that co-culture could efficiently improve extracellular polymeric substances production, electron transfer , and microbial activity . Meanwhile, the mineralization mechanism suggested that acetate was the dominant intermediate which had an inhibitory effect on HJM-8, and co-culture was conducive to mineralization due to the high performance of acetate conversion and Na + K + -ATPase vitality. Besides, a pathway of DMAC biodegradation was proposed for co-culture: DMAC was degraded into acetate by HJM-8, then the accumulated acetate was mineralized by YBH-X. Additionally, the co-culture system was further optimized by Box-Behnken design.
研究领域:
环境微生物学
·
生物修复
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2025
·
IF 5.1
Jixiang Gong,Xuri Wu,Xin He,Feng Tan
The coexistence of nanoplastics and organic pollutants in aquatic environments is common and leads to combined effects on organisms. This study investigated the biological effects of zebrafish embryos subjected to individual and combined treatments of polystyrene nanoplastics (PSNPs) and perfluorooctane sulfonate (PFOS) by analyzing morphological changes, as well as proteomic and metabolic responses. Exposure to 200 μg L−1 PSNPs and/or 100 μg L−1 PFOS at 48 hour post-fertilization (hpf) for six days resulted in increased deformities in zebrafish embryos, including spinal deformities, pericardial edema, and other abnormalities. As the exposure time increased, the combined exposure group demonstrated additive/synergistic embryolethal effects. Proteomic analysis revealed changes in protein expression levels, with 439, 421, and 692 proteins showing altered expression in the PSNPs, PFOS, and co-exposure groups, respectively. These proteins are primarily associated with protein synthesis and transport, energy metabolism, and cardiac muscle contraction. Specifically, in the combined exposure group, certain key proteins related to cardiac muscle contraction, such as sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA2a) and tropomyosin (TPM), showed increased expression, possibly as an adaptive response to pericardial edema. Metabolic analysis revealed that differential metabolites caused by the exposures were mainly involved in the synthesis and metabolism of amino acids, carbohydrates, lipids, and nucleotides, indicating extensive metabolic disruption in the embryo. This study offers insights at the protein and metabolite levels into the biological effects of individual and combined exposures to PSNPs and PFOS, providing evidence of the ecological risks associated with environmental exposure to PFOS and PSNPs.
研究领域:
环境毒理学
·
水生生态学
·
分子生物学
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发育生物学
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生态毒理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Bioengineered
·
2022
·
IF 6.832
Yujie Xu,Dingmao Wang,Guodong Zhao
Proteasome 26S non-ATPase subunit 7 (PSMD7) and forkhead box P3 (FOXP3) have been found to be both upregulated in gastric cancer tissues. FOXP3 was also predicted to have binding sites on PSMD7 promoter. Thus, this study investigated the relationship between PSMD7 and FOXP3 and their roles in gastric cancer. Bioinformatic databases predicted PSMD7 expression in non-cancerous gastric tissue and gastric cancer tissue, as well as the correlation between PSMD7 and the overall/disease free survival. PSMD7 expression in non-cancerous gastric tissue or cells and gastric cancer tissue or cells was detected by qPCR and Western blot. After PSMD7 downregulation by siRNA interference, cell viability, colony-forming capacity and cell apoptosis were analyzed with cell counting kit-8 assay, colony formation assay and terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling. Proliferation and apoptosis markers were assayed by qPCR and Western blot. Dual-luciferase reporter and chromatin immunoprecipitation assays were performed to look at the binding relationship between FOXP3 and PSMD7 promoter. Cell proliferation and apoptosis were examined again after co-transfection of PSMD7 siRNA plasmid and FOXP3 overexpression plasmid. PSMD7 expression was much higher in gastric cancer tissue and cell lines. Interference with PSMD7 decreased gastric cancer cell viability, inhibited their proliferation and colony formation and promoted cell apoptosis. FOXP3 was found to bind to PSMD7 promoter and activate PSMD7 expression. Overexpression of FOXP3 could rescue the effects of PSMD7 knockdown on gastric cancer cells. PSMD7 is involved in the proliferation and apoptosis of gastric cancer cells and can be transcriptionally regulated by FOXP3.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Jia-Gen Cui,Hao Zhang,Ming-Shan Chen,Jia-Xin Wang,Yi Zhao,Jin-Long Li
Phthalates (PAEs), especially di (2-ethylhexyl) phthalate (DEHP), are generally considered to have adverse impact on nervous system. The residue of DEHP in the environment has gradually become a widely concerned environmental problem due to its widespread use in plastic items. Lycopene (LYC) as the readily available natural antioxidant is considered to have the potential to alleviate exogenous poisons-induced nerve damage. However, there is currently a lack of strategies to alleviate the neurotoxicity caused by DEHP, and it is also unknown whether LYC can alleviate the neurotoxicity caused by DEHP. The experiment demonstrated that LYC had the potential to mitigate DEHP-induced mitochondrial damage in cerebellum. DEHP induced the disorder of Ca 2+ transport in cerebellum, thereby resulting in the imbalance of protein homeostasis. Such disruption in protein homeostasis further results in the overactivation of mitochondrial unfolded protein response (UPR mt ) and mitochondrial injury. Mechanistically, LYC could alleviate the imbalance of calcium homeostasis and protein homeostasis induced by DEHP via regulating inositol 1, 4, 5-trisphosphate receptor type1 (IP3R1) and sarco/endoplasmic reticulum Ca (2+)-ATPase 2 (SERCA2), further alleviating mitochondrial damage in cerebellum. Subsequently, the present study suggested the mechanism of cerebellar injury induced by DEHP, and provided a novel approach to treating DEHP-induced neurotoxicity.
研究领域:
神经科学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2020
·Vol. 12
·期 31
·16451-16461
·
IF 6.895
Yuqian Jiang,Zheng Wang,Wei Duan,Lingyan Liu,Mahan Si,Xiaoyuan Chen,Chen-Jie Fang
Multidrug resistance (MDR) remains a huge obstacle during cancer treatment. One of the most studied MDR mechanisms is P-glycoprotein (P-gp) mediated drug efflux. Based on the three-dimensional structural characteristics of P-gp, gold nanoparticles (AuNPs) with average sizes of 4.1 nm and 5.4 nm were designed for the construction of nanodrug delivery systems (NanoDDSs), with the anticancer molecules 2-(9-anthracenylmethylene)-hydrazinecarbothioamide (ANS) and 6-mercaptopurine (6-MP) modified on the AuNP surfaces through the thiol group. In vitro cytotoxicity results suggested that the larger sized AuNPs can effectively decrease the drug resistance index of MCF-7/ADR cells to ∼2. Verapamil and P-gp antibody competitive experiments, combined with the cellular uptake of AuNPs, indicated that larger NanoDDSs were more conducive to intracellular drug accumulation and thus had improved anticancer activities, due to a size mismatch between the nanoparticles and the active site of P-gp, and, therefore, reduced drug efflux was seen. Measurements of ATPase activity and intracellular ATP levels indicated that the larger nanoparticles do not bind well to P-gp, thus avoiding effective recognition by P-gp. This was further evidenced by the observation that 4.1 nm and 5.4 nm NanoDDS-treated MCF-7/ADR cells showed remarkable differences in energy-related metabolic pathways. Therefore, the critical size of AuNPs for overcoming MDR was identified to be between 4.1 nm and 5.4 nm. This provides a more accurate description of the composite dimension requirements for NanoDDSs that are designed to overcome MDR.
研究领域:
癌症研究
·
药学
·
纳米技术
·
细胞生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Du Qi,Fang Chuangdong,Qi He,Benjakul Soottawat,Aubourg Santiago P.,Zhang Bin
Phosphates and trehalose are widely accepted additives in animal muscle products. In this study, the effects of pre-soaking with sodium tripolyphosphate (STPP) and trehalose under vacuum permeation (VP) conditions on the physicochemical properties of shrimp muscle were evaluated over 120 d of frozen storage. The results indicate the STPP/trehalose-VP treatments significantly reduced the thawing loss and prevented changes in the texture, myofibrillar protein (MP) content, and Ca2+-ATPase activity of shrimp muscle during frozen storage compared with results of control and individual STPP or trehalose soaking treatments. The histological structure analysis revealed the permeated STPP/trehalose distinctly inhibited the dissociation of muscle fibers and reduced physical damage to connective tissues during storage. Furthermore, analysis of the thermal properties indicated STPP/trehalose treatment increased the Tg’ values of shrimp muscle tissues, likely by restricting the mobility of water molecules in muscle tissues and embedding proteins in the glassy matrix. Thus, the physical destruction caused by ice crystal growth was greatly reduced, due to the absence of water molecules around muscle proteins during frozen storage. Accordingly, the combined STPP/trehalose-VP treatment significantly enhanced the stability of frozen shrimp, and the results support the application of traditional cryoprotective additives. The treated shrimp can be stored at comparatively higher temperatures with limited physicochemical reactions during frozen storage.
研究领域:
食品科学
·
食品化学
·
食品保藏
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR CELL
·
2023
·
IF 16
Dongmei Tang,Yijun Chen,Hao Chen,Tingting Jia,Qiang Chen,Yamei Yu
In response to the persistent exposure to phage infection, bacteria have evolved diverse antiviral defense mechanisms. In this study, we report a bacterial two-component defense system consisting of a Sir2 NADase and a HerA helicase. Cryo-electron microscopy reveals that Sir2 and HerA assemble into a ∼1 MDa supramolecular octadecamer. Unexpectedly, this complex exhibits various enzymatic activities, including ATPase, NADase, helicase, and nuclease, which work together in a sophisticated manner to fulfill the antiphage function. Therefore, we name this defense system "Nezha" after a divine warrior in Chinese mythology who employs multiple weapons to defeat enemies. Our findings demonstrate that Nezha could sense phage infections, self-activate to arrest cell growth, eliminate phage genomes, and subsequently deactivate to allow for cell recovery. Collectively, Nezha represents a paradigm of sophisticated and multifaceted strategies bacteria use to defend against viral infections.
研究领域:
微生物学
·
分子生物学
·
结构生物学
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）