Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 14
·2089-2103
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IF 4.776
Yi Lin,Yidi Yang,Jianqin Yan,Jun Chen,Jun Cao,Yuji Pu,Li Li,Bin He
In this study, redox/ATP switchable theranostic nanoparticles (TNs) with precise specificity and controllable mobility were developed for the real-time monitoring of the release of an anticancer drug. A fluorescent probe (FAM) and a quencher (BHQ-1) were covalently conjugated to one end of an adenosine-5′-triphosphate (ATP) aptamer and its complementary DNA (cDNA), respectively. Then, doxorubicin (DOX) was intercalated within the DNA duplex to form a stable physical conjugate (FBA@DOX). Poly(ethylene glycol)-block-poly (aspartic acid-graft-cystamine) (PAS), a glutathione-sensitive cationic polymer, was synthesized and complexed with the FBA@DOX, endowing it with excellent stability in physiological solutions. Fluorescence recovery/quenching, DNase degradation, in vitro drug release, cellular uptake, and intracellular trafficking results revealed that the TNs remained in the “OFF” state, with a minimal FAM fluorescent signal and negligible DOX premature release, in low-glutathione and/or low-ATP environments. In contrast, the TNs turned “ON” and rapidly released FBA@DOX in glutathione-rich environments after internalization in cancer cells. The intracellular ATP triggered the conformational changes in FBA@DOX, thereby enabling the controlled release of DOX and simultaneous recovery of the fluorescence for monitoring the DOX release. In a cytotoxicity and apoptosis study, the redox/ATP switchable TNs demonstrated strong anticancer effects, attributable to their selective release of the drug. Overall, our findings may offer a promising strategy for developing a new generation of “smart” theranostic platforms.
研究领域:
生物医学工程
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药物递送系统
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2022
·Vol. 22
·期 7
·2425
·
IF 3.847
Xin Chen,Yangkun Feng,Haohan Chen,Yuting Zhang,Xiaoli Wang,Nandi Zhou
Owing to the significant roles of adenosine triphosphate (ATP) in diverse biological processes, ATP level is used to research and evaluate the physiological processes of organisms. Aptamer-based biosensors have been widely reported to achieve this purpose, which are superior in their flexible biosensing mechanism, with a high sensitivity and good biocompatibility; however, the aptamers currently used for ATP detection have a poor ability to discriminate ATP from adenosine diphosphate (ADP) and adenosine monophosphate (AMP). Herein, an ATP-specific aptamer was screened and applied to construct a fluorescent aptasensor for ATP by using graphene oxide (GO) and strand displacement amplification (SDA). The fluorescence intensity of the sensor is linearly related to the concentration of ATP within 0.1 μM to 25 μM under optimal experimental conditions, and the detection limit is 33.85 nM. The biosensor exhibits a satisfactory specificity for ATP. Moreover, the experimental results indicate that the biosensor can be applied to determine the ATP in human serum. In conclusion, the screened aptamer and the biosensor have promising applications in the determination of the real energy charge level and ATP content in a complex biological system.Keywords:ATP;aptamer;graphene oxide;strand displacement amplification;molecular beacon;fluorescent sensor
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
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2021
·
IF 4.889
Wei Lang,Gengjinsheng Cheng,Rongpeng Peng,Qian-Yong Cao
A fluorescent poly (norbornene)-based copolymer probe P1 , which was obtained via ring-opening metathesis polymerization (ROMP) of a PEG-anchored norbornene monomer NB-PEG with a rhodamine-functionalized monomer NB-RB, has been designed and synthesized. Owing to its amphiphilic property, copolymer P1 self-assembles nanostructure aggregations in aqueous solution with a weak emission. Upon addition of ATP , visual color change from colorless to red and a large fluorescence enhancement were observed for the ring-opening of rhodamine spirolactam mechanism. This probe shows a good photostability, and has been successfully applied for imaging of ATP level in living SMMC cells.
研究领域:
材料科学
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分析化学
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生物医学工程
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细胞生物学
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产品关联
DOI
高影响因子
mSphere
·
2025
·
IF 3.1
Xinda Li,Xiaoyi Luo,Bin Wang,Lei Fu,Xi Chen,Yu Lu
Drug-resistant tuberculosis (DR-TB) urgently requires safer, more accessible alternatives to bedaquiline (BDQ), which faces critical flaws like cardiotoxicity, high costs, and emerging resistance. WX-081, a promising BDQ alternative, has demonstrated superior anti-TB activity and improved safety in clinical studies. However, its mechanism of action remains unexplored, underscoring the need for further research to optimize its potential in advancing global TB elimination efforts. This study reveals WX-081’s dual mechanisms: targeting atpE to disrupt ATP synthase and proton motive force via resistance screening, gene sequencing, and functional assays while enhancing host immunity through macrophage transcriptomics. Molecular docking confirmed atpE binding sites, and immune activation pathways (NF-κB/MAPK) were identified, positioning WX-081 as a potent, safe anti-DR-TB candidate despite unresolved mechanistic details.
研究领域:
微生物学
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药物研发
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免疫学
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产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
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2020
·Vol. 31
·期 5
·1164-1171
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IF 2.578
Mengmeng Song,Meng Zhou,Hengfeng Hu,Heqing Fu
First, attapulgite-Fe 3 O 4 magnetic filler (ATP-Fe 3 O 4 ) was prepared by using a chemical precipitation method. Subsequently, graphite oxide (GO) was prepared through Hummer method, and then reduced GO (RGO) was prepared through GO reduced by chitosan (CS). Finally, a series of WPU-RGO/ATP-Fe 3 O 4 /CS composites were prepared by introduced RGO/ATP-Fe 3 O 4 /CS to waterborne polyurethane. The structure and properties were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffraction (XRD), vibrating sample magnetometry (VSM), thermogravimetric analysis TGA, conductivity test, and tensile test. The experimental results indicated that thermal stability and tensile strength of nanocomposites were improved with the increase of the content of RGO/ATP-Fe 3 O 4 /CS. Meanwhile, with the increase of the RGO/ATP-Fe 3 O 4 /CS content, the electrical and magnetic properties of WPU-RGO/ATP-Fe 3 O 4 /CS composites were improved. When the content of RGO/ATP-Fe 3 O 4 /CS was 8 wt%, the electrical conductivity and the saturation magnetic strength of WPU-RGO/ATP-Fe 3 O 4 /CS composites were 3.1 × 10 −7 S·cm −1 and 1.38 emu/g, respectively. WPU-RGO/ATP-Fe 3 O 4 /CS composites have excellent electrical and magnetic properties.
研究领域:
材料科学
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纳米技术
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2017
·Vol. 185
·期 2
·385-395
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IF 1.751
Cao Hao,Li Chengcheng,Zhao Jing,Wang Fang,Tan Tianwei,Liu Luo
Glutathione (GSH) is an important reducing agent in the living cells. It is synthesized by a two-step reaction and requires two molecules of adenosine triphosphate (ATP) for one molecule GSH. The enzymatic cascade reaction in vitro is a promising approach to achieve a high titer and limit side reactions; although, a cost-effective phosphate donor for ATP regeneration is required. Triphosphate (PolyP (3) ), tetraphosphate (PolyP (4) ), and hexametaphosphate (PolyP (6) ) were investigated in this study. Triphosphate inhibited the bifunctional GSH synthetase (GshF) from Streptococcus agalactiae , while no significant inhibition was observed by adding hexametaphosphate. The polyphosphate kinase from Corynebacterium glutamicum was hence investigated to use hexametaphosphate for regeneration of ATP. Further, the orthogonal experiment, which includes seven factors (buffer concentration, pH value, ADP concentration, GshF dosage, polyphosphate kinase (PPK) dosage, reaction temperature, substrate ratio of amino acid, and reaction times), indicated that the capacity of buffer is the most significant factor of the reaction conditions for enzymatic production of glutathione coupling with a PPK-based ATP regeneration system. After optimizing the Mg 2+ concentration, the reaction was scaled up to 250 mL in a stirred reactor with pH feedback control to stabilize the pH value of reaction system and nitrogen protection to avoid the oxidation of product. A yield of 12.32 g/L was achieved. This work provided a potential GshF-based enzymatic way coupling the PPK-based ATP regeneration to product GSH in the optimal conditions towards cost-effectiveness at the industrial scale.
研究领域:
生物化学
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酶工程
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工业微生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Ting Zhou,Min Fan,Ruiyun You,Yudong Lu,Luqiang Huang,Yunchao Xu,Shangyuan Feng,Yang Wu,Huiying Shen,Lanjin Zhu
We have successfully prepared a highly sensitive sandwich nanosensor combined Fe 3 O 4 and [email protected] @Ag nanorods for histamine detection based on surface-enhanced Raman spectroscopy (SERS). The Fe 3 O 4 beads with –COOH served as a capture part to enrich histamine. The [email protected] @Ag core-shell nanorods functionalized with Nalpha,Nalpha-Bis(carboxymethyl)- l -lysine (AB-NTA) were then used to connect with the imidazolyl group of histamine, simultaneously the internal standard 4-aminothiophenol (4-ATP) in the core-shell structure was used as the SERS signal. PLS regression model based on concentration range 10 −3 -10 −8 mol/L showed a linear trend with R 2 = 0.9907. Our new approach can quickly and reliably determine histamine in fish sample and RAW264.7 cell lysates. This protocol for histamine extraction and SERS analysis enables the development of ultra-sensitive method for histamine detection.
研究领域:
分析化学
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纳米技术
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2020
·Vol. 412
·期 11
·2529-2536
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IF 3.637
Zhang Baozhu,Wei Chunying
A simple turn-on fluorescence strategy is proposed for the detection of ATP based on DNA-stabilized copper/silver nanoclusters (DNA-Cu/Ag NCs). The fluorescence intensity of DNA-Cu/Ag NCs increases significantly in the presence of ATP, because the specific interaction between ATP and its aptamer causes two darkish Cu/Ag NCs to be situated at the 5′ and 3′ termini close to each other. A limit of detection (LOD) of 7.0 μM is found, in a linear range of 2–18 mM, and the proposed sensor is simple, sensitive, and selective. Additionally, the DNA-Cu/Ag NCs/ATP system is further developed into a sensor for ADA detection and demonstrates a linear response to ADA from 5 to 50 U/L with a LOD of 5 U/L. The proposed method is also shown to be successful in detecting ATP and ADA in a solution of fetal bovine serum.
研究领域:
分析化学
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纳米技术
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生物传感器
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 9
·1-10
·
IF 5.833
Chen Xi-Xi,Hou Mei-Jia,Mao Guo-Jiang,Wang Wen-Xin,Xu Fen,Li Yongfei,Li Chun-Yan
A near-infrared (NIR) fluorescence nanoprobe named RhI-DOX@ZIF-90 has been synthesized by wrapping the guest molecule (RhI and DOX) into ZIF-90 framework. The nanoprobe itself is non-fluorescent and the drug (DOX) is inactive. Upon the addition of ATP, the structure of RhI-DOX@ZIF-90 is degraded. The fluorescence of RhI is recovered and DOX is released. The nanoprobe can detect ATP with high sensitivity and selectivity. There is good linear relationship between the nanoprobe and ATP concentration from 0.25 to 10 mM and the detection limit is 0.10 mM. The nanoprobe has the ability to monitor the change of ATP level in living cells and DOX is released inducing apoptosis of cancer cells. RhI-DOX@ZIF-90 is capable of targeting mitochondria, which provides a basis for improving the efficiency of drug delivery by mitochondrial administration. In particular, the nanoprobe is preferentially accumulated in the tumor sites and detect ATP in tumor mice by fluorescence imaging using near-infrared fluorescence. At the same time, DOX can be released accurately in tumor sites and have good anti-tumor efficiency. So, this nanoprobe is a reliable tool to realize early diagnosis of cancer and improve effect of anticancer drug. Graphical abstract
研究领域:
生物医学工程
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纳米医学
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肿瘤学
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Jie Wei,Yumeng Li,Qingyang Si,Qiao Xiao,Qingmin Chen,Tianhui Jiao,Quansheng Chen,Xiaomei Chen
A label-free, sensitive and reliable electrochemical sensor based on hemin/G-quadruplex (G4) DNAzyme was engineered to determine adenosine triphosphate (ATP) in fish. The nucleic acid sequence (DNA1) including the G4 sequence subunit and anti-ATP aptamer segment is anchored on Au electrodes. The nucleic acid sequence (DNA2) is composed of another G4 sequence fragment and anti-ATP aptamer subunit. The probes DNA1 and DNA2 self-assemble into the active hemin/G4 DNAzyme structure on Au electrodes upon exposure to the target ATP. The determination of the target ATP relied on an increase in the catalytic currents occurring on DNAzyme-anchored electrode via the electrochemical reduction of H 2 O 2 . The proposed strategy enables high-performance and specified determination of the target ATP with a detection limit of 0.04 μmol/L. Specifically, the proposed ATP strategy was further implemented to determine ATP in microorganisms in carp samples. Compared with the classical total viable count (TVC) assay, the proposed ATP assay was feasible to assess the freshness of carp, which expands the applications of hemin/G4 DNAzyme and holds promising potential for freshness assessment in food safety.
研究领域:
分析化学
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食品科学
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电化学
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生物传感器
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产品关联
DOI
高影响因子
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
·
2014
·Vol. 34
·期 5
·1780-1791
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IF 3.55
Zhang Rong,Xiang Yang,Ran Qian,Deng Xiaojun,Xiao Yanni,Xiang Lixin,Li Zhongjun
Aim: The aim of this study was to investigate the mechanisms of Cr6+-induced red blood cells (RBCs) damage. Methods: The effect of Cr6+ exposure on RBCs was evaluated by hemolytic rate and blood gas assays. After exposure to 20 μM Cr6+, the percentage of phosphatidylserine (PS)-exposing cells, intracellular Ca2+, reactive oxygen species (ROS), and ATP levels were evaluated, and cell morphology was observed. RBCs were exposed to Cr6+ in different Ringer solutions to investigate the role of changes of Ca2+, ROS, and ATP levels in eryptosis and morphology. Results: The Cr6+-induced damage of RBCs was dose-dependent. After a 6 h incubation with Cr6+, RBCs exhibited significant Ca2+ influx, ROS increase, ATP depletion, and PS exposure, but displayed no obvious change in morphology at this time point. After 24 h Cr6+ exposure, the RBCs decreased in size, appeared to be spike-like, and had decreased forward scatter. Inhibition of Ca2+ influx attenuated PS-exposure caused by 6 h Cr6+ exposure, but did not prevent 24 h Cr6+ exposure-induced morphological change in RBCs. Inhibition of rapid ATP consumption using adenine significantly ameliorated Cr6+-caused PS-exposure at 12 h, 24 h and 48 h, and prevented 24 h Cr6+ incubation-induced morphological change in RBCs. Conclusion: Cr6+ can lead to eryptosis. Ca2+ influx, increased ROS levels, and rapid ATP consumption are closely related to Cr6+-induced RBCs damage. Ca2+ influx plays an extremely important role in Cr6+-mediated toxicity
研究领域:
毒理学
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血液学
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DOI
PubMed
高影响因子
Polymer Chemistry
·
2021
·Vol. 12
·期 8
·1125-1135
·
IF 5.582
Yanfen Jiang,Shuqi Dong,Guoyang Qin,Li Liu,Hanying Zhao
This study reports the facile synthesis of a phenylboronic acid-functionalized block copolymer containing tertiary sulfoniums via the combination of reversible addition–fragmentation chain transfer polymerization of a methionine-derived methacrylate monomer and the alkylation of thioether. The block copolymer self-assembled to form positively charged spherical micelles in aqueous media. Because of the deboronation and subsequent benzyl 1,6-elimination reaction in the presence of H2O2, the micelles displayed an oxidation-responsive feature. Triggered by H2O2, the micelles underwent an expansion, and the surface charge decreased due to the transformation of cationic sulfoniums to neutral sulfurs. Moreover, the micelles can interact with adenosine triphosphate (ATP) via the formation of reversible boronates and electrostatic interactions. In the presence of ATP, the block copolymer micelles spontaneously contracted to small aggregates of ATP/copolymer complexes. Taking advantage of the cationic sulfoniums, the block copolymer co-assembled with negatively charged bovine serum albumin (BSA) to fabricate protein-entrapped biohybrid micelles, driven by multiple electrostatic interactions. BSA in the micelles retained 94% of the native BSA bioactivity. Triggered by H2O2, the BSA-entrapped micelles underwent destruction because of the charge conversion of sulfonium cations to neutral sulfurs. The BSA-entrapped micelles have good biocompatibility and demonstrate an H2O2 and ATP dual-responsive release profile.
研究领域:
高分子化学
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生物材料
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药物递送
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）