JOURNAL OF PHARMACY AND PHARMACOLOGY
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2025
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IF 3.2
Yao Wang,Hua Dong-Ming,Wang Wen-kai,Zhang Zhao-zhou,Guan Yun-Feng,Wang Yan
ObjectiveTo investigate the mechanism of ginsenoside Rb1 (G-Rb1) against colorectal cancer under chronic stress.MethodsA chronic restraint stress (CRS) model and a colorectal cancer (CRC) subcutaneous xenograft model were established. Western blot analysis quantified β2-adrenergic receptor (β2-AR), cAMP response element-binding protein 1 (CREB1), and p-CREB1 expression. Additionally, glycolytic enzymes GLUT1, HK2, and PFKP were analyzed via Western blot and RT-qPCR, with glucose uptake, lactate, ATP, and stress hormone levels assessed by flow cytometry, kits, and ELISA.Key findingsCompared to the control group, the stress group exhibited increased tumor volume and mass, along with elevated expression of β2-AR, p-CREB1, and upregulated expression levels of GLUT1, HK2, and PFKP. Additionally, glucose, lactate, and epinephrine levels were higher in the stress group. In comparison to the stress group, G-Rb1 treatment demonstrated reduced tumor volume and mass, decreased p-CREB1 expression, as well as downregulated protein and mRNA levels of GLUT1, HK2, and PFKP. Glucose, lactate, and epinephrine levels also showed a reduction in the G-Rb1-treated groups.ConclusionsG-Rb1 suppresses the growth of colorectal cancer under chronic stress, potentially through downregulation of the β2-AR/CREB1 signaling pathway, thereby reducing glycolytic activity in colorectal cancer under chronic stress.
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Nature Communications
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2026
·Vol. 17
·期 1
·4225
·
IF 18.1
Wang Yu-Meng,Jia Yanyan,Wu Yu,Zhao Qiaohang,Guo Yumeng,Zhang Li,Jin Min,Zheng Yufang
Elevated prenatal androgen levels have been associated with an increased incidence of several neurodevelopmental conditions in offspring. However, the underlying mechanisms remain poorly understood. Here we showed that elevated prenatal androgens, modeled via aromatase inhibition in mice, disrupt cortical development and behavior in male offspring by activating androgen receptors (AR). AR interacts with MeCP2 to upregulate the transcription factor MEF2C, bypassing standard androgen response elements. Knocking down Mecp2 or Mef2c , or blocking AR, reversed these effects. Mecp2 duplication alone also upregulated MEF2C, driving similar neurogenesis and behavioral changes that were reversed by AR blockade. This reveals a MeCP2-mediated, non-canonical pathway by which androgens may contribute to male-biased neurodevelopmental conditions.
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PLASMA CHEMISTRY AND PLASMA PROCESSING
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2024
·Vol. 44
·期 5
·1951-1969
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IF 2.6
Siyuan Sui,Guohua Ni,Hongmei Sun,Ling Kong,Tao Sun
In this paper, a novel method is presented to enhance the antimicrobial properties of polyvinyl chloride (PVC) via plasma-induced grafting of quaternary ammonium (QA). The results show that the content of oxygen-containing functional groups on PVC surface is significantly increased after Ar-O 2 plasma treatment, beneficially enhancing the thickness and adhesion of QA coating. Plasma treatment time critically affects the morphology of PVC surface, which is closely related to the number, depth and diameter of pit on the surface. With the increase of pits size, the PVC surface forms ridge-like protrusions. The coating formed by grafting QA maintains the morphology characteristics of plasma-treated PVC surface. Results of antibacterial test show that specimens have the best antibacterial activity when there are a high and sharp ridge-like structure of QA coating on the plasma-treated PVC surface.
研究领域:
生物材料学
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表面科学
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高分子化学
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抗菌材料
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医学工程
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JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
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2020
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IF 3.442
Yihan Liu,Lina Kong,Xin Guo,Jianping Xu,Shaobo Shi,Lan Li
The construction of surface defects on semiconductor oxide photoanodes has been identified as a promising route for attaining improved photoelectrochemical (PEC) performance. Here, Ar plasma treatment has been used to etch the surface of hydrothermally synthesized WO 3 nanoplate array films, which generated surface oxygen vacancies (O v s) with the concomitant reduction of surface W 6+ to W 5+ /W 4+ . As a result, the visible-light absorption and photogenerated charge separation of WO 3 was enhanced, as evidenced by X-ray photoelectron spectroscopy (XPS), UV/Vis diffuse-reflectance spectroscopy (DRS), and photoluminescence (PL) spectroscopy. A WO 3 photoanode subjected to Ar plasma treatment for 120 s exhibited a photocurrent density of 1.32 mA cm −2 and an O 2 evolution rate of 1.0 μmol cm −2 ·min −1 under simulated solar light irradiation at a bias potential of 1.23 V vs. reversible hydrogen electrode, approximately 1.7- and 5-times higher, respectively, than those of a pristine sample. This improvement is mainly attributed to the facilitative effect of surface oxygen vacancy defects, which promote electron–hole generation and separation rates and decrease the interfacial charge-transfer resistance between the WO 3 photoanode and electrolyte. Our Ar plasma surface modification also has potential for developing other photoanodes for efficient PEC performance.
研究领域:
材料科学
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能源科学
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JOURNAL OF HAZARDOUS MATERIALS
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2020
·
IF 9.038
Y.L. Zhao,G.G. Li,H.M. Hou,J.C. Shi,S.N. Luo
We investigate atomic and molecular emission of laser-ablated nitromethane in an Ar or N 2 buffer gas, with fs laser-induced breakdown spectroscopy. The electronic bands of CN, C 2 , and NH molecules and the atomic transition lines of C I, N I, and H α are identified. The time series of the emissions are obtained, and the formation mechanisms of CN and C 2 are deduced. The CN violet system, the B 2 Σ + -X 2 Σ + (0-0) band, is chosen to extract plasma temperature from the experimental spectra.
研究领域:
物理化学
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光谱学
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Asia-Pacific Journal of Chemical Engineering
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2012
·Vol. 8
·期 4
·567-577
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IF 0.758
Ying Gu,Xiaowei Liu,Bo Zhao,Wenqiang Liu,Minghou Xu
Nitrogen species distribution during coal pyrolysis with Ca addition in N 2 , CO 2 and Ar was studied at 1000 °C using a horizontal tube furnace. In particular, the effect of Ca on the distribution of nitrogen species in N 2 and CO 2 atmospheres, which were closer to pyrolysis processes of conversional air and oxy-fuel combustion in comparison with inert Ar environment, was investigated for the first time in this work. It was found that Ca has a great effect on nitrogen species distribution during both coal and demineralized coal pyrolysis; on the other hand, CO 2 causes the formation of NO. Demineralization increases the formation of HCN and char nitrogen, but there is little effect on NH 3 formation during pyrolysis in N 2 , in contrast to pyrolysis in Ar atmosphere, which decreases NH 3 formation. A significant reduction in char yield was found after char gasification in CO 2 at 1000 °C. With two different Ca-based additives, i.e., (CH 3 COO) 2 Ca and Ca(OH) 2 , char yield decreases with the increasing amount of Ca additives in both cases. The effect of Ca on HCN and NH 3 formation during pyrolysis depends on both the form of Ca and the amount of addition. In most cases, HCN and NH 3 formation decreases with Ca addition in Ar, but increases in N 2 . Ca addition slightly promotes HCN formation during pyrolysis in CO 2 . There is no NH 3 detected during pyrolysis in CO 2 because of the reaction of NH 3 and CO 2 in the background. In addition, NO concentration decreases sharply with increasing the amount of Ca addition in the atmosphere of CO 2 , which means that Ca has an effect not only on pyrolysis, but also on gasification of char in CO 2 . In most cases, char nitrogen increases with Ca addition in N 2 or Ar. © 2012 Curtin University of Technology and John Wiley & Sons, Ltd.
研究领域:
能源科学
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环境科学
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JOURNAL OF ETHNOPHARMACOLOGY
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2022
·
IF 5.195
Ying-Li Cai,Feng Zhang,Xiu-Xiu Dou,Hua-Wu Zeng,Gao-Song Wu,Yan-Lin Liang,Xi-Ke Xu,Jing Zhao,Ji Ye,Wei-Dong Zhang
Ethnopharmacological relevance Dingkun Pill (DKP), a traditional Chinese medicine prescription, was modified from Bujing decoction and Xusijiangsheng pill by the imperial physician in the Qing dynasty (1700′ s). It was believed to treat various gynecological diseases by nourishing qi and blood. Accumulating evidence indicates that it is effective in treating polycystic ovary syndrome (PCOS). However, the therapeutic efficacy and mechanism of action DKP against PCOS need to be further elucidated. Aim of the study To investigate the therapeutic effect and action mechanism of DKP against PCOS using an integrated approach of metabolomics and network pharmacology . Materials and methods The rat model of PCOS was established by dehydroepiandrosterone . An integrated metabolomics and network pharmacology strategy was applied to systemically clarify the mechanism of DKP against PCOS. Theca cells were prepared to evaluate the effect of DKP and its ingredients on testosterone synthesis in vitro. Results The pharmacological experiments demonstrated that DKP could effectively convert the disordered estrous cyclicity, decrease the level of testosterone and the luteinizing hormone/follicle stimulating hormone ratio, and inhibit abnormal follicle formation in PCOS rats. By metabolomics analysis, 164 serum endogenous differential metabolites and 172 urine endogenous differential metabolites were tentatively identified. Steroid hormone biosynthesis and ovarian steroidogenesis were the most significantly impacted pathways. Based on network pharmacology and metabolomics studies, the ingredient-target-pathway network of DKP in the treatment of PCOS was constructed. Among the 10 key targets, CYP17A1 , CYP19A1 , STS, AR, ESR1, and MYC were closely involved in ovarian androgen synthesis. In theca cell-based assay of testosterone synthesis, DKP and its two active compounds (ligustilide and picrocrocin) showed inhibitory effects. Conclusion DKP effectively improved symptoms in rats with dehydroepiandrosterone-induced PCOS. The mechanism of DKP in the treatment of PCOS is related to the CYP17A1 enzyme required for androgen synthesis.
研究领域:
药理学
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中医
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妇科
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代谢组学
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网络药理学
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Nature Communications
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2023
·Vol. 14
·期 1
·1-8
·
IF 16.6
Chi Hongbin,Liu Yang,Li Ziyi,Chen Wanxin,He Yi
Flexible covalent organic frameworks (COFs) have been studied for applications containing sorption, selective separation, and catalysis. How to correlate the microscopic structure with flexibility in COFs is a great challenge. Herein, we visually track the flexible deformation behaviors of single COF-300 and COF-300-AR particles in response to solvent vapour guests with dark-field microscopy (DFM) in an in operando manner. COF-300-AR with freely-rotating C-N single bonds are synthesized by the reduction of imine-based COF-300 consisting of rigid C=N double bonds without changing topological structure and crystallinity. Unexpectedly, we observe that the flexible deformation of COF-300 is extremely higher than that of COF-300-AR despite it bears many C-N single bonds, clearly illustrating the apparent flexibility decrease of COF-300 after reduction. The high spatiotemporal resolution of DFM enables the finding of inter-particle variations of the flexibility among COF-300 crystals. Experimental characterizations by variable-temperature X-ray diffraction and infrared spectroscopy as well as theoretical calculations demonstrate that the flexible deformation of COF-300 is ascribed to the pedal motion around rigid C=N double bonds. These observations provide new insights into COF flexibility.
研究领域:
材料科学
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化学
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纳米技术
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Advanced Science
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2026
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IF 14.1
Zhengquan Zhu,Yihan Wang,Haiyun Chen,Xinye Yu,Tingyu Wang,Yajing Weng,Meihong Guo,Ying Huang,Gaojian Tao,Wangsen Cao,Yong Wang,Daojuan Wang
Persistent androgen receptor (AR) activation is an important contributor to polycystic ovary syndrome (PCOS) and is affected by transcriptional regulation via histone acetylation; however, the underlying mechanisms are partially understood. This study demonstrated that AR activation in ovarian granulosa cells (GCs) of both dehydroepiandrosterone (DHEA) and high-fat diet-induced PCOS mouse models correlated with a significant increase in the histone acetyltransferase p300 and histone acetylation. Conversely, GC-specific p300 knockout or pharmacological inhibition with C646/A-485 effectively reduced AR activation, histone 3 acetylation (H3K18ac/H3K27ac), and ovarian fibrosis in PCOS mice, highlighting p300 as a critical driver. ATAC-seq and RNA-seq identified “open” chromatin regions at the AR promoter in PCOS ovaries, corresponding with increased AR transcription and histone acetylation. p300, along with transcription factor SP1 and the acetyl-reader BRD4, bound to H3K18ac and H3K27ac of the AR promoter in PCOS-modeled ovaries and GCs, which was blocked by C646 and the SP1 inhibitor Plicamycin, respectively. Importantly, continuous AR activation by its ligand DHT largely diminished the anti-fibrotic and ovarian-protective effects of C646. These findings suggest that p300, SP1, and BRD4, form a critical transcriptional complex driving AR activation and PCOS development, and that targeting the p300/AR axis may present a promising therapeutic approach for treating PCOS.
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ANALYST
·
2025
·
IF 3.3
Yue Cao,Longfei Lai,Qingyuan Bao,Yiru Sheng,Peng Ye,Zhaogang Teng,Yang Zhou
In recent years, the overuse and improper disposal of antibiotics have led to their widespread presence in various environmental matrices and biological fluids. Exploring non-noble metal sulfides as electrocatalysts for reliable antibiotic detection holds substantial value but is limited by their inherent low electrochemical activity. Herein, CoS2 nanoparticles were first synthesized via a hydrothermal method, and used for the in situ assembly of ZnIn2S4 nanosheets. Then, plasma technology was employed for efficient and rapid generation of sulfur vacancies. Detailed studies illustrated the formation of heterojunctions, facilitating electron transfer from the thin, large-area ZnIn2S4 to CoS2, which acts as a co-catalyst due to its inherent conductivity and metallicity. The plasma-assisted generation of vacancies resulted in the formation of numerous exposed metal catalytic centers. Also, both the heterojunction and vacancy construction afforded a narrowed energy gap, an upregulated conduction band, and an enhanced carrier density, favoring electron injection during the electro-reduction process. Thus, an electrochemical platform was established using this material as an electrode substrate for furazolidone (Fz) detection, achieving a detection limit of 1.2 nM. The constructed platform was finally applied to evaluate Fz in actual samples of river water and urine, thanks to its good selectivity, high stability, and acceptable repeatability. In addition, in situ Fourier transform infrared technology was also used to monitor the electrocatalytic process. This work paves the way for preparing low-cost non-precious metal sulfides, which can be used as promising candidates for electrocatalysts in antibiotic testing.
研究领域:
环境科学
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材料科学
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分析化学
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PROGRESS IN ORGANIC COATINGS
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2025
·
IF 7.3
Xinglei Cui,Zhonglian Li,Yizhuo Wang,Long Li,Zhi Fang
The weather resistance capability of insulating materials, i.e. surface performance including hydrophobicity, mechanical and electrical strength under diverse weather conditions, is considered of critical importance for high-voltage devices working outdoors. In this study, atmospheric pressure plasma jet with Ar/Hexamethyldisiloxane (HMDSO) assisted by γ-Aminopropyltriethoxysilane (APTES) and oxygen (O 2 ) addition is employed to deposit a robust insulating film with good weather resistance capability on the surface of Polymethylmethacrylate (PMMA). The gas flow rates of Si-containing precursors (HMDSO/APTES) and auxiliary gas (O 2 ) are varied to elucidate the correlation between precursor ratio and film performances. The mechanism for robust film deposition is explored by investigating reactions of active particles deduced from discharge characteristics, surface physical morphology and chemical composition variations. The results indicate that APTES plays a pivotal role in the enhancement of the film mechanical strength, while O 2 can further improve both mechanical and electrical performances. A robust superhydrophobic film is deposited, inhibiting the reduction in water contact angle by 81.6 % after the tape peel test compared with the film deposited by Ar/HMDSO system, and the flashover voltage is increased by 38.2 % with the optimal HMDSO/APTES/O 2 ratio of 7:5:1. The simultaneous improvements of the film hydrophobic, mechanical and electrical performances are contributed by crosslinking of silicone deposits and the introduction of -NH₂ groups. The weather resistance capability of the insulating material is effectively enhanced, providing guidance for outdoor insulation applications.
研究领域:
材料科学
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电气工程
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JOURNAL OF PLANT GROWTH REGULATION
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2022
·Vol. 42
·期 3
·2006-2022
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IF 4.64
Wang Junhong,Cheng Jun-Hu,Sun Da-Wen
Previous studies have shown the great potential of using plasma-activated water (PAW) on improving agriculture seed germination, however, information on the influence of PAW on crop plantlet juice remains scanty. In this research, the effect of PAW generated by atmosphere pressure Ar–O 2 plasma jet for 1–5 min on wheat seed germination, seedling growth and nutritional properties of wheat plantlet juice was investigated. Results revealed that all PAWs could enhance wheat seed germination and seedling growth in 7 days by improving the germination rate, germination index, fresh weight, dry weight and vigour index, and especially that PAW activated for 3 min (PAW-3) showed the best overall performance. In addition, the application of PAWs enhanced the nutritional properties of wheat plantlet juice from those grown for 14 days by improving total soluble solids, protein content, photosynthetic pigments, total phenolic content, antioxidant activity, enzyme activity, free amino acids and minerals content, and the best enhancement was also observed in PAW-3. It was concluded that PAWs would be an effective technique to enhance the growth and nutritional properties of crop sprouts, which could be served as functional foods in many forms.
研究领域:
农业科学
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植物生理学
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食品科学
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