ChemNanoMat
·
2020
·Vol. 6
·期 5
·765-772
·
IF 3.384
Di Zhang,Qi Wang,Xiaocui Cheng,Huanhuan Li,Qiuyue Wang,Fangfang Yu,Wenying Wang,Jing Xin,Qingmei Li,Changfeng Dou,Xiaoqing Wu,Hongli Qian,Wei Fang,Fengrui Wu,Yong Liu,Shuanglin Jiang,Wenyong Li,Rong Wang
Graphical Plasmid DNA coheres to the surface of AuNPs through electrostatic adsorption of the PEI mixture with a certain proportion of high-molecular-weight (25,000) PEI (HMW PEI) and low-molecular-weight (1,800) PEI (LMW PEI). AuNPs−PEI−DNA shows a very low toxic impact to embryo development and excellent efficiency of delivering exogenous DNA into mouse early embryonic cells. A transgenic system of noninvasive cytosolic transport based on exogenous plasmid DNA (pIRES2−EGFP) loading on gold nanoparticles (AuNPs) coated with polyethyleneimine (PEI) was successfully constructed, which can remove the technical threshold and possible damage of microinjection to oocytes and embryo development. In this study, mouse zygotes were cultured in KSOM medium containing the complex of AuNPs−PEI−DNA (APD) until formation of blastocyts, and then transferred into pseudopregnant female mice for obtaining transgenic offspring. Fluorescence observations confirm the expression of GFP in early embryos; importantly, data from identifying experiments by genotyping, immunofluorescence and DNA sequencing show that the GFP sequence indeed integrates into the chromosome of offspring individuals and is expressed. Furthermore, TUNEL test and analysis on cell differentiation of blastocysts indicate an excellent safety of APD to the development of embryos. All these results suggested that AuNPs, being an alternative technique to traditional microinjection, can efficiently introduce biomolecules into early embryonic cells without the prerequisite of expensive micromanipulators and skilled technicians, which will greatly promote study of life sciences in various aspects.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Yang Zhou,Mingyu Ma,Hanping He,Zhiwei Cai,Nan Gao,Chaohui He,Gang Chang,Xianbao Wang,Yunbin He
Detection of nitrite is important for environmental safety and human health, and the development of high-performance sensors for accurate detection of nitrite is highly desirable. Herein, a highly sensitive graphene electrochemical transistor (GECT) nitrite sensor was designed and fabricated for the first time. A single layer of graphene was placed between the source and drain electrodes by the wetting transfer method to act as channel for the transistor. Au nanoparticles modified reduced graphene oxide nanocomposites (AuNPs/RGO) were electrodeposited at the transistor gate to improve its catalytic oxidation performance of nitrite with optimized electrodeposition conditions. The sensing principle was attributed to changes in effective gate voltage applied to GECT induced by electrooxidation of nitrite at gate electrodes . Due to the high carrier mobility of graphene in the channel and the excellent electrocatalytical activity of AuNPs/RGO on the gate, the obtained sensor device exhibited an exceedingly low detection limit (0.1 nM nitrite) and ultra-wide linear range from 0.1 nM to 7 μM and from 7 to 1000 μM, which are comparable or superior to the performance of large-scale instruments (e.g. chromatography , spectrophotometry , and spectrofluorimetry etc.). The GECT device also showed good anti-interference performance toward common interfering ions and stable performances. Nitrite in natural lake water has been proven to be monitored by our devices. Therefore, the present novel GECT sensor could act as a desirable practical platform for highly sensitive detection of nitrite in the food and environmental fields.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Kunhao Yang,Zhongqiu Li,Yueguang Lv,Chuyi Yu,Peilong Wang,Xiaoou Su,Li Wu,Yujian He
An electrochemical aptasensor for ultrasensitive detection of hydroxylated polychlorinated biphenyl (OH-PCB) was developed. In this work, the sulfydryl aptamer selected for OH-PCB was easily immobilized on the surface of the modified glassy carbon electrode via Au-S bond due to incorporation of gold nanoparticles/poly dimethyl diallyl ammonium chloride-graphene composite. The fabrication process of the electrochemical aptasensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry . The peak currents obtained by differential pulse voltammetry decreased with the increasing of OH-PCB concentrations. The current responded approximately logarithmically to the OH-PCB concentration ranging from 2.9 × 10 −11 M to 2.9 × 10 −7 M with excellent linear correlation performance. The detection limit of OH-PCB was estimated as low as 5.3 × 10 −12 M. The developed method had been successfully applied to analyzing lake water. Particularly, the method of the electrochemical aptasensor opens up a new perspective in environmental monitoring by the aptamer identification.
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NANOTECHNOLOGY
·
2024
·Vol. 36
·期 4
·045501
·
IF 2.9
Liyun Ding,Pan Wang,BaoQuan Xiao,Zelin Gao,Dekui Zhang,Tuanjie Che,Juanjuan Zuo,Yumai Li
Enrofloxacin (ENR), as a synthetic broad-spectrum antibiotic is widely utilized in veterinary medicine to treat animal diseases and promote livestock growth, it can inhibit bacterial DNA gyrase subunit A, thereby preventing bacterial DNA replication and exerting its antibacterial effect. However, excessive use of enrofloxacin poses significant risks to ecological balance and human health due to residual contamination. We have developed a novel ENR aptamer sensor based on the gold nanoparticles/aptamer (AuNPs-Apt) complexes, in which AuNPs were synthesized via the seed method and functionalized with aptamers. The optical properties, particle size, functional groups and morphology of the AuNPs-Apt probe were characterized by transmission electron microscope, Fourier transform infrared spectrometer and UV–vis spectrophotometer, respectively. The aptamer biosensor can specifically identify enrofloxacin, with a wide detection range (0.05–100 μg ml−1) and a good linear relationship ({R^2}=0.99) within the detection range. In addition, the biosensor also has the advantages of short detection time, low biological toxicity, good stability, and low detection cost. Therefore, it shows a great prospect for practical application in the field of detecting enrofloxacin residues.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
San-Jun Shi,Rong-Fu Huang,Zi-Yan Fan
Development of rapid and sensitive methods for screening of large number of chemical pollutants with potential genotoxicity is in urgent demands. In this work, we reported a chemical genotoxicity sensor based on the lateral flow strip (LFS) quantification of the 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo). The proposed sensing strategy introduced human 8-oxoguanine DNA glycosylase (hOGG 1) and human apyrimidinic endonuclease (APE 1) to convert 8-oxodGuo to strand break. Then, the DNA conjugated gold nanoparticles (AuNPs) complexes could not be captured on test line (T-line), showing visible red color fading. Additionally, the mean grey value of the optical density in the T-line zone was analyzed by using a mobile phone camera and Image J software to quantify 8-oxodGuo. Under the optimized experimental conditions, the LFS DNA biosensor could detect the lowest concentration of 8-oxodGuo down to 0.2 pmol. To demonstrate its universal applications, in vitro generation of 8-oxodGuo by Rose Bengal under irradiation and Fe 2+ -mediated Fenton reagent were successfully measured. The detection limit of oxidative DNA damage induced by Rose Bengal or Fenton reagent could be down to 0.01 μM or 1 nM Fe 2+ /4 nM H 2 O 2 , respectively. The 10-fold higher sensitivity for Fenton reagent was attributed to much more severe damage than Rose Bengal, producing both DNA strand break and nucleobases conversion. The results demonstrated that the LFS DNA biosensor could detect oxidative DNA damage for the first time, showing its potential application for assessment of the genotoxicity of abundant chemical pollutants.
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ANALYTICAL SCIENCES
·
2024
·
IF 1.8
Cai Tongji,Chen Meilun,Yang Jie,Tang Chunhua,Lu Xiaoling,Wei Zheng,Jiang Hanbing,Hou Yucui,Zhao Jia,Yu Peng
Abstract Vitamin D 3 (VD 3 ) is the main form of vitamin D and an essential nutrient for maintaining human life. Currently, traditional methods for detecting 25-hydroxyvitamin D3(25(OH)D 3 ) are complex and expensive. In this study, we constructed an accurate, sensitive, simple, and cost-effective label-free biosensor based on an aptamer for the detection of 25(OH)D 3 . The aptamer-modified sulfhydryl adopted self-assembly as a way to stably immobilize at the glassy carbon electrode (GCE) surface modified by gold nanoparticles (AuNPs). Upon 25(OH)D 3 binding to the aptamer, the complexes inhibit electron transfer at the electrode surface, leading to reduced [Fe(CN) 6 ] 3−/4− redox peak current. Consequently, the quantity of 25(OH)D 3 that interacts with the electrode-bound aptamer correlates with the observed electric current response values. The Aptamer/AuNPs/GCE aptasensor achieved direct and highly sensitive detection of 25(OH)D 3 over a wide concentration range (1.0–1000 nM), with a limit of detection of 1.0 nM. At the same time, other molecules with a similar structure, such as 25(OH)D 2 , Vitamin D 3 , and Vitamin D 2 , had lower response interference than 25(OH)D 3 . Therefore, this biosensor has great potential to become a portable diagnostic device for 25(OH)D 3 . Graphic abstract
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Analytical Methods
·
2024
·Vol. 16
·期 8
·1281-1287
·
IF 2.7
Keyu Li,Peng Xiao,Ningning Yuan,Shujuan Yan,Pei Zhao,Guowei Zuo
MiRNAs are critical regulators of target gene expression in many biological processes and are considered promising biomarkers for diseases. In this study, we developed a simple, specific, and sensitive miRNA detection method based on proximity ligation reaction, which is easy to operate. The method uses a pair of target-specific DNA probes immobilized on the same gold nanoparticles (AuNPs), which hybridize to the target miRNA. Hybridization brings the probes close together, allowing the formation of a continuous DNA sequence that can be amplified by Quantitative Real-time PCR (qPCR). This method eliminates the need for complex reverse transcription design and achieves high specificity for discriminating single base mismatches between miRNAs through a simple procedure. This method can sensitively measure three different miRNAs with a detection limit of 20 aM, providing high versatility and sensitivity, even distinguishing single-base variations among members of the miR-200 family with high selectivity. Due to its high selectivity and sensitivity, this method has important implications for the investigation of miRNA biological functions and related biomedical research.
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高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Xinrui Feng,Qinwei Xu,Yan Liu,Sijia Wang,Yong Cao,Chen Zhao,Shuai Peng
Deoxynivalenol (DON) is a common mycotoxin in food that mainly pollutes grain crops and feeds, such as barley, wheat and corn. DON has caused widespread concern in the field of food and feed safety. In this study, a colorimetric immunoassay was proposed based on the aggregation of gold nanoparticles (AuNPs) due to the decomposition of Mn 2+ from gold-coated manganese dioxide (AuNP@MnO 2 ) nanosheets. In this study, 2-(dihydrogen phosphate)- l -ascorbic acid (AAP) was hydrolyzed by alkaline phosphatase (ALP) and converted to ascorbic acid (AA). Then, AuNP@MnO 2 was reduced to Mn 2+ and AuNPs aggregation occurred. Using the unique optical characteristics of AuNPs and AuNP@MnO 2 , visible color changes realized simple detection of DON with high sensitivity and portability. With increasing DON content, the color changed more obviously. To quantitatively detect DON, pictures can be taken and the blue value can be read by a smartphone. The detection limit (Ic10) of this method was 0.098 ng mL −1 , which was 326 times higher than that of traditional competitive ELISA, and the detection range was 0.177–6.073 ng mL −1 . This method exhibited high specificity with no cross-reaction in other structural analogs. The average recovery rate of DON in corn flour samples was 89.1 %–110.2 %, demonstrating the high accuracy and stability of this assay in actual sample detection. Therefore, the colorimetric immunoassay can be used for DON-related food safety monitoring.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qinghao Song,Zhihao Huang,Ying Xiao,Yu Zhang,Liyi Li,Yi Yu,Si Chen,Qing Wu
MXene Ti 3 C 2 has emerged as a promising biosensing material due to the high surface area, excellent biocompatibility, tunable functional groups (-F, O, -OH), and outstanding electrical conductivity, effectively addressing issues such as low sensitivity and inadequate biocompatibility in sensors. This work, MXene Ti 3 C 2 -AuNPs are synthesized as functional materials through a one-step synthesis method. We propose the first all-fiber biosensor featuring a laterally offset SMF-MMF-SMF-MMF-SMF (SMSMS) interferometric structure functionalized with MXene Ti 3 C 2 -AuNPs as the substrate of the biosensor sensor device, for the detection of the alkane degradation (ALK) gene, to the best of our knowledge. The biosensor enables in-situ target DNA (tDNA) detection across 1 fM to 1 μM concentrations based on this nanoengineered interface, demonstrating 1755.95 nm/RIU sensitivity and a 1 fM limit of detection (LOD) – Two orders of magnitude lower than conventional optical DNA sensors (μM-level). The sensor exhibits a wavelength shift of 1.23 nm in response to complementary DNA, which is 8.2 times greater than that for non-complementary DNA, with a maximum wavelength shift error of 0.11 nm across three repeated experiments and a shift within 0.1 nm over 7 days for sensors with attached functional MXene Ti 3 C 2 , demonstrating excellent specificity, stability, and reproducibility. These advantages render the fiber optic device coated with MXene Ti 3 C 2 -AuNPs an effective solution for ALK gene monitoring and broaden its applicability for ultra-trace DNA detection in environmental and biomedical contexts
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Shuyan Xiao,Jiping Lu,Liang Sun,Shengli An
A new colorimetric biosensor for specific detection of azlocillin was developed by using DNA aptamer as recognition element and unmodified gold nanoparticles (AuNPs) as colorimetric indicator. In the absence of azlocillin, the AuNPs were protected by the aptamer and stabilized at high NaCl concentrations, displaying a red solution. In the presence of azlocillin, the aptamer reacts specifically with azlocillin, resulting in the aggregation of AuNPs and an apparent red to blue color change. The characteristic change can be easily observed by the naked eye and quantitatively detected by an ultraviolet–visible (UV–Vis) spectrometer. Under the optimal conditions, the absorbance variation at 522 nm (ΔA 522 ) of AuNPs changed proportionally with increasing concentration of azlocillin, which exhibited a linear relationship in the concentration range of 50 nM to 500 nM, with a detection limit of 11.6 nM. Furthermore, the aptasensor was successfully used to detect azlocillin in milk and tap water samples, with recoveries ranging from 97.64% to 102.21% and a relative standard deviation (RSD) less than 3.81%.
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TALANTA
·
2011
·
IF 3.722
Zhihua Wang,Hui Li,Jing Chen,Zhonghua Xue,Bowan Wu,Xiaoquan Lu
A novel electrochemical sensor based on molecularly imprinted polymer film has been developed for aspirin detection. The sensitive film was prepared by co-polymerization of p -aminothiophenol ( p -ATP) and HAuCl 4 on the Au electrode surface . First, p -ATP was self-assembled on the Au electrode surface by the formation of Au–S bonds. Then, the acetylsalicylic acid (ASA) template was assembled onto the monolayer of p -ATP through the hydrogen-bonding interaction between amino group ( p -ATP) and oxygen (ASA). Finally, a conductive hybrid membrane was fabricated at the surface of Au electrode by the co-polymerization in the mixing solution containing additional p -ATP, HAuCl 4 and ASA template. Meanwhile, the ASA was spontaneously imprinted into the poly-aminothiophenol gold nanoparticles (PATP–AuNPs) complex film. The amount of imprinted sites at the PATP–AuNPs film significantly increases due to the additional replenishment of ASA templates. With the significant increasing of imprinted sites and doped gold nanoparticles , the sensitivity of the molecular imprinted polymer (MIP) electrode gradually increased. The molecularly imprinted sensor was characterized by electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). The linear relationships between current and logarithmic concentration were obtained in the range from 1 nmol L −1 to 0.1 μmol L −1 and 0.7 μmol L −1 to 0.1 mmol L −1 . The detection limit of 0.3 nmol L −1 was achieved. This molecularly imprinted sensor for the determination of ASA has high sensitivity, good selectivity and reproducibility, with the testing in some biological fluids also has good selectivity and recovery.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 39
·18718-18723
·
IF 3.925
Rui Shi,Jin Yang,Shiqi Cheng,Shangying Qin,Li Huang,Yilin Wang,Yuanjin Xu
AuNPs@MoS2 NSs with high peroxidase-like activity were prepared and used for the colorimetric determination of biothiols. The synergistic effect between gold nanoparticles (AuNPs) and MoS2 nanosheets (MoS2 NSs) accelerated the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2 to form the blue oxidized TMB (oxTMB) with an absorption peak at 652 nm. However, the AuNPs@MoS2 NS-catalyzed TMB oxidation could be inhibited by biothiols, resulting in an absorbance decrease. Under the optimized experimental conditions, the signal responses to homocysteine (Hcy), glutathione (GSH), and cysteine (Cys) were linear in the range from 1.0 to 100 μmol L−1, and the limit of detection of these biothiols was 0.93, 0.71, and 0.73 μmol L−1, respectively. The suggested method was successfully applied for the determination of Hcy in human serum samples with a recovery of 97.7–101.2%, indicating the potential application of this method for biothiol sensing in real samples.
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