CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Qingru Chen,Ying Liu,Xiaohui Liu,Qingzhe Xu,Zheng Sun,Caili Zhang,Yongxin Li,Bin Zhao,Dawei Gao,Yan Zhang,Binbin Huang,Shenghai Huang,Maozhen Han
A novel SMX-degrading strain, Bacillus cereus SDB-X , was isolated and achieved high SMX degradation. • Strain SDB-X degrades SMX through acetylation, desulfonylation, and hydroxylamination pathways. • Adaptive mutation in the key gene menC increased enzyme–substrate binding affinity, lowering the binding energy from −8.3 to −8.6 kcal/mol. • Long-term acclimation upregulated the expression of key degradation genes, facilitating the adaptive evolution of SDB-X's SMX-degrading capability. • In complex aqueous matrices, the SDB-X strain demonstrated consistent SMX removal efficiency. Sulfamethoxazole (SMX), a widely distributed emerging contaminant, poses risks to ecosystems and public health. Microbial degradation offers an efficient and eco-friendly remediation strategy. In this study, a SMX-degrading strain was isolated from hospital wastewater and identified as Bacillus cereus SDB-X. The original strain (SDB-X00) degraded 89% of 50 mg/L SMX within five days, and after 60 days of acclimation under SMX stress, the degradation efficiency increased to 96%. Kinetic analysis revealed that the acclimated strain SDB-X60 exhibited the fastest degradation rate ( k = 0.721 d −1 , t ₁/₂ = 0.96 d). Moreover, SDB-X60 maintained stable degradation activity in a complex wastewater matrix. LC-MS/MS analysis proposed a tentative SMX metabolic pathway involving N -acetylation and typical intermediate metabolites. Genomic analysis identified several candidate degradation-related genes; a mutation in SDB-X60 altered the MenC protein substrate binding free energy from −8.3 to −8.6 kcal/mol. Transcriptomic analysis further indicated upregulation of degradation-related genes after acclimation. This study expands understanding of SMX biodegradation by B. cereus and highlights the role of acclimation in enhancing degradation activity, providing a theoretical basis for bioremediation of antibiotic-contaminated environments.
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CHIRALITY
·
2018
·Vol. 30
·期 7
·907-912
·
IF 1.833
Jianyong Zheng,Xing Lan,Lijuan Huang,Yinjun Zhang,Zhao Wang
D-alanine is widely used in medicine, food, additives, cosmetics, and other consumer items. Esterase derived from Bacillus cereus WZZ001 exhibits high hydrolytic activity and stereoselectivity. In this study, we expressed the esterase gene in Escherichia coli BL21 (DE3). We analyzed the biocatalytic resolution of N-acetyl-DL-alanine methyl ester by immobilized whole E . coli BL21 (DE3) cells, which were prepared through embedding and cross-linking. We analyzed biocatalytic resolution under the optimal conditions of pH of 7.0, temperature of 40°C and substrate concentration of at 700 mM with an enantiomeric excess of 99.99% and e.e. p of 99.50%. The immobilized recombinant B . cereus esterase E . coli BL21 (DE3) cells exhibited excellent reusability and retained 86.04% of their initial activity after 15 cycles of repeated reactions. The immobilized cells are efficient and stable biocatalysts for the preparation of N-acetyl-D-alanine methyl esters.
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PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Yanmei Li,Lun Luo,Wenhai Wang,Bin Hong,Yi Ma,Jufang Wang
Synergistic antibacterial effect as a promising strategy against foodborne pathogens is compelling since the development of novel antibacterial agents is not so easy. Here we investigated the synergistic effect of cell wall hydrolase Lys14579 and cinnamaldehyde (CAL) against emetic Bacillus cereus . The MICs of Lys14579 and CAL against emetic B. cereus were 25 and 200 μg/mL, respectively. The combination of Lys14579 and CAL displayed a synergistic antimicrobial effect with a Fractional Inhibitory Concentration Index (FICI) of 0.375. Bactericidal assays revealed that this combination had better bactericidal performance than either substance alone. Furthermore, the combination of Lys14579 and CAL effectively inhibited the biofilm formation of emetic B. cereus at the concentrations of 6.25 and 50 μg/mL, respectively. Additionally, it down-regulated the cereulide synthetase gene ( cesB ) expression at the transcription level and reduced swarming motility by 55%. Scanning electron microscope and PI uptake assays further demonstrated that the combination accelerated the disruption of emetic B. cereus cells. Finally, the synergistic preservative effect of this combinational was observed in boiled rice and fresh lean beef. Thus, the combination of Lys14579 and CAL represents a potential synergistic strategy for controlling emetic B. cereus contamination in the food industry.
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RSC Advances
·
2023
·Vol. 13
·期 23
·15379-15390
·
IF 3.9
Nan Xu,Li-hua Du,Yan-chao Chen,Jin-hao Zhang,Qian-feng Zhu,Rong Chen,Guo-ping Peng,Qi-ming Wang,Hua-zhong Yu,Li-qun Rao
Lonicera japonica Thunb. has attracted much attention for its treatment of bacterial and viral infectious diseases, while its active ingredients and potential mechanisms of action have not been fully elucidated. Here, we combined metabolomics, and network pharmacology to explore the molecular mechanism of Bacillus cereus ATCC14579 inhibition by Lonicera japonica Thunb. In vitro inhibition experiments showed that the Lonicera japonica Thunb.'s water extracts, ethanolic extract, luteolin, quercetin, and kaempferol strongly inhibited Bacillus cereus ATCC14579. In contrast, chlorogenic acid and macranthoidin B had no inhibitory effect on Bacillus cereus ATCC14579. Meanwhile, the minimum inhibitory concentrations of luteolin, quercetin, and kaempferol against Bacillus cereus ATCC14579 were 15.625 μg mL−1, 31.25 μg mL−1, and 15.625 μg mL−1. Based on the previous experimental basis, the metabolomic analysis showed the presence of 16 active ingredients in Lonicera japonica Thunb.'s water extracts and ethanol extracts, with differences in the luteolin, quercetin, and kaempferol contents between the water extracts and ethanol extracts. Network pharmacology studies indicated that fabZ, tig, glmU, secA, deoD, nagB, pgi, rpmB, recA, and upp were potential key targets. Active ingredients of Lonicera japonica Thunb. may exert their inhibitory effects by inhibiting ribosome assembly, the peptidoglycan biosynthesis process, and the phospholipid biosynthesis process of Bacillus cereus ATCC14579. An alkaline phosphatase activity assay, peptidoglycan concentration assay, and protein concentration assay showed that luteolin, quercetin, and kaempferol disrupted the Bacillus cereus ATCC14579 cell wall and cell membrane integrity. Transmission electron microscopy results showed significant changes in the morphology and ultrastructure of the cell wall and cell membrane of Bacillus cereus ATCC14579, further confirming the disruption of the cell wall and cell membrane integrity of Bacillus cereus ATCC14579 by luteolin, quercetin, and kaempferol. In conclusion, Lonicera japonica Thunb. can be used as a potential antibacterial agent for Bacillus cereus ATCC14579, which may exert its antibacterial activity by destroying the integrity of the cell wall and membrane.
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PubMed
高影响因子
CATALYSIS LETTERS
·
2019
·Vol. 149
·期 2
·610-618
·
IF 2.372
Qin Yan-Li,Ruan Tao,Hou He-Shuai,Hou Ya-Li,Yang Zhong-Hua,Quan Can
Carbonyl reductase, as biocatalyst, is very important to chiral alcohols production through asymmetric reduction of carbonyl compound. A novel thermal stable carbonyl reductase from Bacillus cereus (BcCR) dependant on NADPH was obtained through a new genome mining strategy proposed in this work. By analyzing its amino acid sequence and structure, the BcCR should be a thermal stability and wide pH tolerance carbonyl reductase. Its gene was cloned by PCR with B. cereus genomic DNA as template. Its heterologous expression system, E. coli BL21 (DE3) plysS/pET28a- bccr , was constructed, and BcCR was successfully expressed. Enzymatic properties show that at 57.5 °C and pH 7.0 it can reach maximum reaction rate. Its K m and V max to ethyl 4-chloroacetoacetate is 1.85 mmol/L and 0.22 µmol/(min·mg protein ), respectively. It can catalyze the asymmetric reduction of the β-carbonyl compound, such as ethyl 4-chloroacetoacetate to ethyl S -4-chloro-3-hydroxybutyrate. This paper proposes a practical method for discovery of new carbonyl reductases, and provides a novel enzyme as biocatalyst for asymmetric reduction of β-carbonyl compound.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Cheng Zhang,Qinghai Zhang,Ming Luo,Qiuping Wang,Xiaomao Wu
Butylated hydroxytoluene (BHT), as an emerging contaminant in ecosystems, has potential influences on animals, aquatic organisms, and public health, and has been proven to be a major allelochemical of Pinellia ternata . In this study, Bacillus cereus WL08 was used to rapidly degrade BHT in liquid culture. Strain WL08 immobilized on tobacco stem charcoal (TSC) particles notably accelerated BHT removal in contract to its free cells, and exhibited excellent reutilization and storage capacities. The optimal removal parameters of TSC WL08 were ascertained to be pH 7.0, 30 °C, 50 mg L −1 BHT and 0.14 mg L −1 TSC WL08. Moreover, TSC WL08 significantly accelerated the degradation of 50 mg L −1 BHT in sterile and non-sterile soils compared to that of free WL08 or natural dissipation, and notably shortened their half-lives by 2.47- or 362.14- fold, and 2.20- or 14.99- fold, respectively. Simultaneously, TSC WL08 was introduced into the continuous cropping soils of P. ternata , which accelerated the elimination of allelochemical BHT, and notably enhanced the photosynthesis, growth, yield, and quality of P. ternata . This study provides new insights and strategies for the rapid in situ remediation of BHT-polluted soils and effective alleviation of P. ternata cropping obstacles.
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高影响因子
JOURNAL OF DAIRY SCIENCE
·
2020
·
IF 3.333
Qianying Li,Guoyang Xie,Yutong Wang,Zoraida P. Aguilar,Hengyi Xu
ABSTRACT In this study, a new vancomycin (Van)-modified poly- l -lysine (PLL) magnetic bead (MB) technique was developed for isolation of gram-positive bacteria. The method combines magnetic separation with a multiplex PCR (mPCR) assay and gel electrophoresis for easy and rapid detection of Bacillus cereus . Vancomycin was used as a molecular ligand between the MB and the d -alanyl- d -alanine moieties on the cell wall surface of B. cereus . The PLL served as a flexible molecular tether between the MB and Van that reduced steric hindrance maintaining the biological activity of Van. The MB-PLL-Van capture nanoprobes exhibited excellent capture and isolation efficiency for B. cereus in spiked milk matrix samples without interference from the complex food matrix. The subsequent mPCR assay showed high specificity for the 4 target genes in B. cereus , the entFM , cesB , cer , and 16S rRNA genes, that were used to achieve efficient genotyping and detection. Under optimum conditions, the limit of detection reached 10 3 cfu/mL, with a dynamic range of detection at 10 3 to 10 7 cfu/mL in pure culture. Application of the MB-PLL-Van mediated mPCR assay for B. cereus in milk matrix samples achieved results similar to those of the pure culture. In addition, with a 6-h pre-enrichment of B. cereus that was spiked in milk matrix samples, the limit of detection reached 10 1 cfu/mL. The MB-PLL-Van mediated mPCR assay developed in this study could be used as a universal technology platform for the efficient enrichment and genotyping of gram-positive bacteria.
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Jun Li,Fu-Yu Liu,Bao-Lin He,Rui Cui,Hong Wu
Carvacrol has great antibacterial activity against Bacillus cereus , and its action mechanism was comprehensively studied in this work. It could not only control the growth of B. cereus by affecting the integrality of cellular structure and physiological metabolism, but also inhibit the swarming motility, biofilm formation, and toxins production. To increase the stability of carvacrol during the application, the sodium casein (SC)/hydroxypropyl- β -cyclodextrin (HPCD)/carvacrol nanoemulsion was fabricated. The mean particle size and polydispersity index of the nanoemulsions were 113.8 nm and 0.23, respectively, at SC and HPCD concentrations of 2% and 1%. FTIR analysis demonstrated that carvacrol was successfully encapsulated into the nanoemulsions. The MIC and MBC of nanoemulsions against B. cereus were 0.15 and 0.30 mg/mL, respectively, which were lower than those of free carvacrol (0.19 and 0.38 mg/mL). Carvacrol in nanoemulsions showed a slow and sustained release behavior. Furthermore, the nanoemulsions had good stability (particle size <200 nm) after storage at 4 °C and 25 °C for 15 days. Notably, the nanoemulsions were effective in controlling the growth of B. cereus in whole, low-fat, and skim milk and had better antimicrobial activity than free carvacrol. The results indicated that carvacrol nanoemulsions have potential in the antibacterial application of the food industry.
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2026
·
IF 5.7
Gaolei Bai,Jing Gao,Shizi Zhang,Liumi Meng,Ren Chu,Jichang Zhang,Lijuan Luo,Lingyan Jiang
The search for natural and safe alternatives to synthetic fungicides is crucial for controlling postharvest anthracnose in papaya, a disease primarily caused by Colletotrichum species. This study identifies Bacillus cereus ZW21 as a potent biocontrol agent and delineates the mechanism of its volatile organic compounds (VOCs) directly in a fruit system. In vivo assays on papaya fruit demonstrated that ZW21 VOCs significantly reduced lesion area and disease incidence by up to 80.95% and 70.46%, respectively. Bioactivity-guided identification revealed hydroxyacetone (HA) and 3-methyl-3-buten-1-ol (ME) as the key antifungal VOCs. These compounds exhibited direct antifungal activity by disrupting fungal membrane integrity, inducing mitochondrial dysfunction, and elevating reactive oxygen species. Beyond direct inhibition, transcriptomic analysis of treated fruit revealed that HA and ME activated host defense pathways, including phenylpropanoid biosynthesis and hormone signaling, while suppressing ripening-related metabolism. A critical distinction was observed: ME enhanced glycolysis and pyruvate metabolism, providing a mechanistic explanation for potential phenolic overflow and fruit blackening, whereas HA avoided this metabolic overdrive. Our findings not only validate the efficacy of a novel biocontrol strain and its defined VOCs but also provide a comprehensive mechanistic framework supporting their application for sustainable postharvest protection.
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PubMed
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Yanmei Li,Zixi Chen,Zigan Xu,Jinmei Wu,Dexin Yang,Yeye Yu,Ting Xie,Yong Dai,Shaodong Luan,Hanchao Gao
The study aims to investigate the antibacterial effect and mechanism of rhein against Bacillus cereus using Tandem Mass Tag (TMT) quantitative proteomics combined with non-targeted metabolomics. The results showed that rhein exhibited strong antimicrobial effect against B. cereus , with a minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of 1.2 μg/mL and 2.4 μg/mL, respectively. Additionally, rhein treatment increased membrane permeability, induced reactive oxygen species generation, and disrupted the cell morphology of B. cereus cells. Notably, rhein could effectively inhibit the proliferation of B. cereus in cooked rice and skimmed milk during the storage at 4 °C. Proteomics and metabolomics analyses identified 288 differentially expressed proteins (DEPs) and 402 differential metabolites (DMs) in rhein-treated B . cereus . These molecules were involved in multiple biological pathways related to primary metabolism, carbon metabolism, amino acid biosynthesis and energy production, as well as the synthesis of secondary metabolites and stress response-related signaling pathways. Bioinformatics analysis further revealed that rhein disrupted key cellular processes, including amino acid metabolism, nucleotide metabolism, glycolysis/gluconeogenesis and flagellar structure. These disruptions significantly impaired bacterial metabolic functions and motility, ultimately inhibiting bacterial growth and leading to cell death. This research provides valuable understanding into the mechanism of rhein's action against B . cereus and lays theoretical foundations for its potential use as an antibacterial agent in the food industry.
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危险品化学品经营许可证（带存储）