Frontiers in Plant Science
·
2023
·
IF 5.6
Pengfei Deng,Ruoyong Yin,Huiling Wang,Leiru Chen,Xiaoqing Cao,Xiaoniu Xu
The intercropping practice has been regarded as a practical land-use selection to improve the management benefits of Bletilla striata plantations. The reports about the variety of economic and functional traits of Bletilla pseudobulb under intercropping systems were limited. The present study investigated the variation of economic and functional traits of Bletilla pseudobulb under different intercropping systems (the deep-rooted intercropping system: B. striata - Cyclocarya paliurus, CB; and the shallow-rooted intercropping system: B. striata - Phyllostachys edulis, PB). The functional traits were analyzed through non-targeted metabolomics based on GC-MS. The results indicated that the PB intercropping system significantly decreased the yield of Bletilla pseudobulb while significantly increasing the total phenol and flavonoids compared with the control (CK). However, there were no significant differences in all economic traits between CB and CK. The functional traits among CB, PB, and CK were separated and exhibited significant differences. Under different intercropping systems, B. striata may adopt different functional strategies in response to interspecific competition. The functional node metabolites (D-galactose, cellobiose, raffinose, D-fructose, maltose, and D-ribose) were up-regulated in CB, while the functional node metabolites (L-valine, L-leucine, L-isoleucine, methionine, L-lysine, serine, D-glucose, cellobiose, trehalose, maltose, D-ribose, palatinose, raffinose, xylobiose, L-rhamnose, melezitose, and maltotriose) were up-regulated in PB. The correlation between economic and functional traits depends on the degree of environmental stress. Artificial neural network models (ANNs) accurately predicted the variation in economic traits via the combination of functional node metabolites in PB. The correlation analysis of environmental factors indicated that Ns (including TN, NH4+-, and NO3--), SRI (solar radiation intensity), and SOC were the main factors that affected the economic traits (yield, total phenol, and total flavonoids). TN, SRI, and SOC were the main factors affecting the functional traits of the Bletilla pseudobulb. These findings strengthen our understanding of the variation of economic and functional traits of Bletilla pseudobulb under intercropping and clarify the main limiting environmental factors under B. striata intercropping systems.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Miaoyan Ren,Liting Niu,Liuyan Pu,Jinhua Wu,Zhilin Xie,Xiao-kun Ouyang,Jun Deng
Developing biomimetic asymmetric wound dressings that mimic the structure and multifunctionality of natural skin remains challenging. Here, we report a natural polysaccharide-based asymmetric hydrogel (MP/AMBGZ) derived from Bletilla striata polysaccharide (BSP) via a facile one-pot, two-step strategy. Methacrylated BSP forms a biocompatible network, while gallic acid provides wet adhesion and antioxidant activity. ZnO nanoparticles incorporated into the inner layer enable Zn 2+ -mediated antibacterial activity and promote angiogenesis. Meanwhile, the outer MA-POSS layer forms a hydrophobic barrier that prevents bacterial adhesion. The hydrogel exhibits good mechanical properties, high swelling capacity, and excellent biocompatibility. In vitro studies demonstrate its cytocompatibility, antibacterial activity, hemostatic performance, and anti-inflammatory effects. In vivo experiments show rapid hemostasis and accelerated wound healing. In infected full-thickness wounds, MP/AMBGZ achieves near-complete closure within 14 days and promotes functional skin regeneration, including hair follicle formation. Overall, this work highlights the potential of BSP-based asymmetric hydrogels as multifunctional wound dressings for clinical applications.
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Frontiers in Pharmacology
·
2022
·
IF 5.988
Tianxiang Zhu,Baifei Hu,Cheng Ye,Haiming Hu,Mingzhu Yin,Zhigang Zhang,Shuiqing Li,Yanju Liu,Hongtao Liu
This study aimed to elucidate the mechanism of Bletilla striata oligosaccharides (BO) in the treatment of ulcerative colitis (UC). A UC mouse model was induced by 3% Dextran sodium sulfate (DSS), and BO (200 mg/kg/d) were administered for intervention. The results show that BO effectively inhibited the release of intestinal inflammatory cytokines such as IL-6, TNF-α, and IL-1β. Also, BO profoundly elevated the secretion of mucins and the expression of tight junction (TJ) proteins to attenuate dysfunction of the intestinal barrier. The 16S rDNA sequencing and liquid chromatography/gas chromatography-mass spectrometer (LC/GC-MS) analysis of mouse feces revealed that BO regulated the disturbance of gut microbiota and intestinal metabolites. By using the in vitro fermentation broth of BO and gut microbiota-depleted mice treated with antibiotics, we confirmed the protection of BO against UC. In conclusion, BO played a role in improving UC by modulating gut microbial composition and intestinal metabolites, which provided new therapeutic strategies for UC treatment.Keywords:
Bletilla striata oligosaccharides, ulcerative colitis, gut microbiota, intestinal metabolites, intestinal barrier
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Hai-Yuan Zhang,Kun-Tang Wang,Yan Zhang,Yuan-Lu Cui,Qiangsong Wang
Skin trauma presents significant treatment challenges in clinical settings. Hydrogels made from naturally-derived polysaccharide have demonstrated great potential in wound healing. Here, a novel in-situ crosslinked self-healing hydrogel was prepared using oxidized Bletilla striata polysaccharide (BSP) and cationic gelatin via a Schiff-base reaction without the need for any chemical crosslinkers. Similar to the natural extracellular matrix, the BSP-gelatin hydrogel (BG-gel) exhibited typical viscoelastic characteristics. The rheological properties, mechanical behavior, porous structure, and degradation performance of BG-gel could be adjusted by changing the aldehyde group content of BSP. Importantly, the hydrogel showed superior hemostatic performance in rat tail amputation and liver incision models. It significantly facilitated wound healing by promoting hair follicles regeneration, blood vessels repair, collagen deposition, and inducing skin tissue remodeling via increased CD31 expression in a full-thickness skin wound rat model. This multifunctional hydrogel holds potential as a wound dressing for skin trauma, offering both hemostasis and expedited healing.
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JOURNAL OF BIOMATERIALS APPLICATIONS
·
2024
·
IF 2.3
Jin Shang,Liangliang Duan,Weimin Zhang,Qibin Zhuang,Xiaomei Ren,Dale Gu
Bletilla striata polysaccharide (BSP) was added to curdlan to form a blend hydrogel through a simple heating-cooling procedure to improve the hydrophilicity and healing efficacy of curdlan-based hydrogel used in wound healing. We explored the interplay between BSP and curdlan, studied how BSP concentration affects the physical properties and microstructures of hydrogels, and examined the biocompatibility and healing properties of the blend hydrogel. It was proved that the hydrogel framework was primarily formed by ordered arranged curdlan molecules, with BSP uniformly dispersed and intertwined with curdlan through hydrogen bonding. This effectively improved its hydrophilicity and strengthened the microstructure. Curdlan was found to be compatible with BSP. The blend hydrogel B3Cd3 (containing 1.5% BSP and 1.5% curdlan, w/v) was identified as the optimal formulation based on its higher water adsorption, water retention, thermal stability and interconnected microstructure, and was thus selected for further research. In vitro experiments revealed the highest cell viability of L929 in B3Cd3 extracts compared to those extracts of single-component curdlan hydrogel (Cd). In vivo, animal studies indicated that the B3Cd3 accelerated wound healing compared to the control group by improving re-epithelialization and blood vessel regeneration. On Days 3 and 11, the therapeutic benefits of B3Cd3 exceeded those of the Cd group, and no significant differences were observed in wound healing rates between the B and B3Cd3 groups from Day 7. The study proves that BSP enhances the physical and healing properties, as well as cell proliferation, of the curdlan-based hydrogel. The blend hydrogel B3Cd3, with its exceptional properties, holds potential for future application as a material for non-infected wound healing.
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PHARMACOLOGICAL RESEARCH
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2020
·
IF 5.893
Baifei Hu,Cheng Ye,Elaine Lai-Han Leung,Lin Zhu,Haiming Hu,Zhigang Zhang,Junping Zheng,Hongtao Liu
As traditional Chinese medicine, Bletilla striata has been widely applied to clinical treatment for its unique pharmacological profiles. This study aimed to investigate the beneficial role of Bletilla striata oligosaccharides (BO) in improving the metabolic syndrome by regulation of gut microbiota and intestinal metabolites. Treatment of HFD-fed mice with BO prevented weight gain, reversed the glucose intolerance and insulin resistance, and inhibited adipocyte hypertrophy. BO-treated mice also suppressed chronic inflammation and protected intestinal barrier from damage. These effects were linked to the reversal of gut microbiota dysbiosis, which contributed to the homeostasis of intestinal metabolites including bile acids, short-chain fatty acids and tryptophan catabolites. The depletion and reconstitution of intestinal flora from BO- or HFD-treated mice confirmed the significance of gut microbiota in regulation of HFD-induced metabolic disorders. We demonstrated for the first time that BO improved metabolic syndrome through the regulation of gut microbiota and intestinal metabolites. The modulation initiated by BO represents a promising strategy for treatment of obesity and related metabolic diseases.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Guofeng Zhong,Mengyu Qiu,Junbo Zhang,Fuchen Jiang,Xuan Yue,Chi Huang,Shiyi Zhao,Rui Zeng,Chen Zhang,Yan Qu
In this study, a novel nanofiber material with Polylactic acid (PLA), natural plant polysaccharides- Bletilla striata polysaccharide (BSP) and Rosmarinic acid (RA) as the raw materials to facilitate wound healing was well prepared through coaxial electrospinning. The morphology of RA-BSP-PVA@PLA nanofibers was characterized through scanning electron microscopy (SEM), and the successful formation of core-shell structure was verified under confocal laser microscopy (CLSM) and Fourier transform infrared spectroscopy (FTIR). RA-BSP-PVA@PLA exhibited suitable air permeability for wound healing, as indicated by the result of the water vapor permeability (WVTR) study. The results of tension test results indicated the RA-BSP-PVA@PLA nanofiber exhibited excellent flexibility and better accommodates wounds. Moreover, the biocompatibility of RA-BSP-PVA@PLA was examined through MTT assay. Lastly, RA-BSP-PVA@PLA nanofibers can induce wound tissue growth, as verified by the rat dorsal skin wound models and tissue sections. Furthermore, RA-BSP-PVA@PLA can facilitate the proliferation and transformation of early wound macrophages, and down-regulate MPO + expression of on the wound, thus facilitating wound healing, as confirmed by the result of immunohistochemical. Thus, RA-BSP-PVA@PLA nanofibers show great potential as wound dressings in wound healing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaojia Zhang,Yingying Mu,Lijie Zhao,Yanlong Hong,Lan Shen
Biomaterials capable of achieving effective sealing and hemostasis at moist wounds are in high demand in the clinical management of acute hemorrhage . Bletilla striata polysaccharide (BSP), a natural polysaccharide renowned for its hemostatic properties, holds promising applications in biomedical fields. In this study, a dual-dynamic-bonds crosslinked hydrogel was synthesized via a facile one-pot method utilizing poly(vinyl alcohol) (PVA)-borax as a matrix system, followed by the incorporation of BSP and tannic acid (TA). Chemical borate ester bonds formed around borax , coupled with multiple physical hydrogen bonds between BSP and other components, enhanced the mechanical properties and rapid self-healing capabilities. The catechol moieties in TA endowed the hydrogel with excellent adhesive strength of 30.2 kPa on the surface of wet tissues and facilitated easy removal without residue. Benefiting from the synergistic effect of TA and the preservation of the intrinsic properties of BSP, the hydrogel exhibited outstanding biocompatibility , antibacterial, and antioxidant properties. Moreover, it effectively halted acute bleeding within 31.3 s, resulting in blood loss of 15.6 % of that of the untreated group. As a superior hemostatic adhesive, the hydrogel in this study is poised to offer a novel solution for addressing future acute hemorrhage, wound healing , and other biomedical applications.
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Foods
·
2026
·
IF 6
Junliang Liu,Jiayao Lu,Peiyi Wang,Chenglin Zhu,Luca Laghi
Natural polysaccharides have considerable potential to modulate the gut microbial ecosystem. This study integrated INFOGEST-based simulated gastrointestinal digestion, in vitro fermentation using rat feces, untargeted metabolomics, and 16S rRNA sequencing to characterize the digestive stability ofBletilla striatapolysaccharides (BSP) and the dose-specific microbial and metabolic responses to BSP. During digestion, BSP exhibited changes in total and reducing sugar content while retaining appreciable antioxidant activity. During fermentation, BSP promoted microbial growth and reduced pH. The medium dose selectively enrichedBifidobacterium, whereas the high dose favoredLactobacillusandLigilactobacillus, accompanied by lower relative abundances of potentially unfavorable taxa, includingEscherichia–ShigellaandProteus. Metabolomic analysis further revealed that BSP predominantly altered amino acid, aromatic amino acid, and carbohydrate metabolism, generating distinct dose-specific metabolic signatures. Overall, BSP remained available for microbial utilization after digestion and reshaped both microbial composition and metabolic profiles in a dose-specific manner, indicating its promise as a functional food ingredient.
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Plants-Basel
·
2025
·Vol. 14
·期 5
·689
·
IF 4.1
Hai Huang,Long Yang,Chunfang Luo,Tuo Qi,Junna Duan
ABletilla striata(Thunb.)Reichb.f., known as Bai Ji in Chinese, is a plant from the Orchidaceae family that has been used for its medicinal properties for thousands of years in China.B. striataholds significant economic value due to its esteemed medicinal applications. Our study aimed to analyze the transcriptome of wildB. striatatubers across multiple years to understand the molecular mechanisms regulating polysaccharide metabolism and tuber enlargement. We collected wildB. striatasamples of different growth ages and analyzed their chemical composition, including total phenols, polysaccharides, alkaloids, and proteins. The results showed that the content of these compounds varied with the growth age of the plants. Our study focused on analyzing the genes associated with growth years and accelerating the seedling growth cycle, which holds immense value for the preservation and optimal utilization of superiorB. striataorchid resources. To further investigate the underlying molecular mechanisms, we performed a comprehensive transcriptome analysis to explore gene expression, functional annotation, and regulatory networks related to the development and chemical composition ofB. striatatubers. The quality of perennial medicinal herbs is intricately linked to their growth age. Unfortunately, excessive wild resource excavation has resulted in the premature harvesting of these herbs, causing a decline in their overall quality and effectiveness. Our study offers valuable insights into the conservation and utilization ofB. striataresources.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xuechun Wang,Yanxiang Sang,Hongjie Zhang,Benyan Zheng,Bibo Wang,Yuan Hu,Ruiying Wu
As a wound management strategy, this study developed a nanocomposite hydrogel based on the photothermal therapy and photodynamic therapy of nanomaterials, as well as the effects of Bletilla striata polysaccharide (BSP). In this research, MXene nanosheets loaded with indocyanine green were embedded into a hydrogel doped with BSP. By combining the photothermal effect and photodynamic effect, the treatment of wounds infected with Staphylococcus aureus was achieved under 808 nm near-infrared light irradiation (NIR). The characterization results of the hydrogel showed that it had good adhesiveness with an adhesive strength of 629 KPa ± 15.52 KPa and excellent biocompatibility; the swelling rate after 48 h was 485.53 % ± 0.26 %. In terms of antibacterial performance, the hydrogel had an antibacterial rate of over 90 % against Staphylococcus aureus . In addition, it could significantly promote the deposition of collagen and angiogenesis in infected wounds. When applied to wound treatment, the wound closure rate reached 98 % after 14 days, the collagen fraction of the healed wound was 87.63 % ± 1.81 %, and the epidermis thickness was 130.33 μm ± 1.25 μm. This indicated that the nanocomposite hydrogel dressing could effectively promote wound healing.
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MOLECULAR PHARMACEUTICS
·
2026
·
IF 4.9
Zhou Yuhao,Yue Zhongqun,Feng Tingting,Zhang Fangxia,Guan Jing,Luo Juanjuan,Tan Yanan,Zhou Ying,Zhu Yue,Wang Zuhua
Gastric cancer is a highly prevalent malignant tumor of the digestive system worldwide. Although andrographolide (AG) and coixol (CX) possess potential anti-gastric cancer effects, their clinical application is restricted by low bioavailability and poor targeting ability. Based on the meridian tropism theory of traditional Chinese medicine and the concept of “integrated drug and carrier”, this study constructed Bletilla striata polysaccharide-vitamin E succinate (BSP-VES) polymeric micelles co-loaded with AG and CX (AG&CX@BSP-VES) for synergistic targeted therapy of gastric cancer. In this study, AG&CX@BSP-VES was first systematically characterized in vitro to clarify its physicochemical properties, drug release behavior and hemolysis profile. Subsequently, using subcutaneous and orthotopic gastric cancer mouse models as research objects, in vitro cellular uptake and in vivo tumor targeting ability efficacy and biological safety of this drug delivery system were further evaluated. Results showed that the micelles were regularly spherical with a particle size of approximately 105 nm, exhibited favorable biological safety and asynchronous differential release characteristics, and could be specifically internalized by cells in vitro (which could be blocked by free BSP). In vivo, the accumulation of micelles in tumor masses was significantly higher than that in normal gastric tissues. Moreover, the cytotoxicity of the micelles against SGC-7901 tumor cells was remarkably higher than that of free AG or CX alone, which was attributed to enhanced cellular drug uptake and the synergistic effect between AG and CX. This study provides novel insights for the precise delivery of active ingredients from traditional Chinese medicine and individualized therapy of gastric cancer.
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