Soft Matter
·
2023
·Vol. 19
·期 25
·4651-4659
·
IF 3.4
Xi Zhao,Jiani Peng,Liangliang Shi,Guoxiang Wang,Yong Gao
The preparation of mixed-shell polymeric nanoparticles (MSPNs) and their stabilized non-aqueous Pickering emulsions was described in this study. Poly(methyl methacrylate)–poly(4-vinylpyridine) (PMMA–P4VP) diblock copolymer nanoparticles with different morphologies including spheres, worms and vesicles were first prepared via reversible addition-fragmentation chain transfer-based polymerization induced self-assembly in toluene. C18 alkyl chains were subsequently grafted onto the surfaces of the as-prepared PMMA–P4VP nanoparticles, affording C18/PMMA–P4VP MSPNs with P4VP blocks as the core and C18/PMMA chains as the mixed shells. MSPNs were utilized as Pickering emulsifiers to prepare non-aqueous Pickering emulsions, where [Bmim][PF6] and toluene oils were selected. Two kinds of different Pickering emulsions, [Bmim][PF6]-in-toluene and toluene-in-[Bmim][PF6], could be formed, depending on the initial location of MSPNs. However, neither of them could be generated when PMMA–P4VP diblock copolymer nanoparticles were adopted as Pickering emulsifiers, indicating MSPNs were better than diblock copolymer nanoparticle precursors in the aspect of stabilizing oil–oil interfaces. The formation mechanisms of different kinds of Pickering emulsions were unraveled in this study.
研究领域:
材料科学
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化学工程
·
胶体科学
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2026
·
IF 4
Jia Chen,Run-qin Wang,Bei-bei Guo,Li Yang,Shuang Hu
A magnetic mesoporous silica (SBA-15)-based dispersive solid phase microextraction was first proposed and introduced for simultaneous analysis of four cardiac glycosides (including deslanoside, lanatoside C, digoxin, and digitoxin) in human plasma coupled with HPLC-UV. In this study, magnetic C 18 -modified SBA-15 (SBA-15/C 18 /Fe 3 O 4 ) was used as the extractant, which was prepared and characterized using a suite of techniques including Fourier transform infrared spectroscopy, transmission electron microscopy, N 2 adsorption-desorption, vibrating sample magnetometer, scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. The influencing parameters of the extraction were investigated and optimized. The adsorption process of SBA-15/C 18 /Fe 3 O 4 followed the Langmuir isotherm and pseudo-second-order kinetic models. The adsorbent also exhibited excellent thermal and chemical stability, alongside good reusability. Under the optimum experimental conditions, the proposed method demonstrated satisfactory performance with good linearity (8-3200 ng/mL for deslanoside and 1.6-640 ng/mL for lanatoside C, digoxin and digitoxin with the correlation coefficients no less than 0.9987), high precision (0.8%
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Liang Qi,Xinting Zhang,Longjiang Li,Xilan Li,Xiangna Chang
Prolonged use of cosmetics containing banned fluoroquinolones can cause skin disorders and increase drug resistance. The QuEChERS method holds considerable application prospect in the pretreatment of cosmetic samples. Although octadecyl (C 18 )-bonded silica sorbent is commonly used as a QuEChERS clean-up material, the conventionally commercially available bulk C 18 sorbent is relatively costly and has a limited purification mechanism. In this study, using dendritic silica sphere (dSiO 2 ) as the substrate, and through the condensation reaction between cyanuric chloride (CNCl) and the amino group, a dNSiO 2 -CNCl-C 18 sorbent was synthesized. The sorbent was characterized using infrared spectroscopy, transmission electron microscopy, surface area and pore size analysis, and water contact angle measurements. The experimental results regarding the matrix removal from the model cosmetic lotion showed that dNSiO 2 -CNCl-C 18 exhibited a higher matrix removal efficiency compared to a commercial C 18 sorbent. Furthermore, a QuEChERS-HPLC-FLD method was developed using dNSiO 2 -CNCl-C 18 for the simultaneous detection of four fluoroquinolones, norfloxacin (NOR), pefloxacin (PEF), enrofloxacin (ENR), and difloxacin (DIF). The linear ranges were 0.10 to 100.0 μg L −1 for NOR and PEF, and 0.05 to 100.0 μg L −1 for ENR and DIF, with coefficient of determination exceeding 0.999. Satisfactory spiked recoveries for the lotion sample ranged from 91.5% to 107.0%, with intra-day and inter-day precision values not exceeding 7.6%. The established method was subsequently validated through its successful application in additional cream and emulsion samples. dNSiO 2 -CNCl-C 18 exhibits low synthesis costs and demonstrates promising potential for matrix purification in various complex samples, facilitating the rapid determination of target compounds.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2022
·
IF 6.51
Yanjuan Huang,Xiuling Dai,Zilin Guan,Di Liu,Lingling Ren,Meixu Chen,Zishan Zeng,Jingwen Jiang,Yong Luo,Yuanfeng He,Min Huang,Chunshun Zhao
Due to a powerful DNA damage repair system and a lack of surface markers, there is currently no effective chemotherapy or tailored targeted therapies available for triple-negative breast cancer (TNBC) treatment. Herein, a tailored DNA damage nanoamplifier ( [email protected] /Pt(IV) C18 ) was engineered to simultaneously induce DNA damage and inhibit DNA reparation for highly efficient TNBC treatment. A newly synthesized Pt(IV) C18 prodrug , the DNA damaging inducer, and the hydrophobic poly(ADP-ribose) polymerases (PARPs) inhibitor niraparib, which is used as the DNA repair blocker, were concurrently encapsulated in highly biocompatible PEGylated liposomes to prepare [email protected] /Pt(IV) C18 , for enhanced cancer therapy and future clinical translation. [email protected] /Pt(IV) C18 with an appropriate size and excellent stability, effectively accumulated at the tumor site. After internalization by tumor cells, niraparib, a highly-selective hydrophobic PARP1 inhibitor, could exacerbate the accumulation of platinum-induced DNA lesions to induce excessive genome damage for synergistic cell apoptosis, which was evidenced by the upregulated γ-H 2 AX and cleaved-PARP levels. Importantly, [email protected] /Pt(IV) C18 exhibited remarkable antitumor efficacy on TNBC without BRCA mutants in vivo with little systemic toxicity. Inspired by the concept of “synthetic lethality”, this study provides an inspirational and clinically transformable nanobased DNA damaging amplification strategy for the expansion of TNBC beneficiaries and highly efficient TNBC treatment via DNA damage induction and DNA repair blocking.
研究领域:
肿瘤学
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纳米技术
·
药理学
·
癌症治疗
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JOURNAL OF CHROMATOGRAPHY A
·
2026
·
IF 4
Jinlin He,Luying Shen,Dunming Xu,Ting Sun,Yan Chen,Jinsheng Hu,Yan Ji,Lijuan Yan
First demonstration of C18-column HILIC-like separation for aminoglycosides. • Hydrophilic retention via water layer & vicinal silanols on base-deactivated silica. • Tandem WCX-C18 SPE protocol developed for effective matrix cleanup. • Robust, MS-compatible, practical monitoring of aminoglycoside residues in foods. An LC–MS/MS method was developed for the simultaneous determination of 16 aminoglycosides (AGs) in pork, chicken, milk, and eggs using a conventional Hypersil base-deactivated silica (BDS) C 18 column operated under HILIC-like conditions. Unlike typical C 18 -based methods that rely on ion-pairing reagents in the LC mobile phase, separation was achieved with an ion-pair-free mobile phase consisting only of 0.1% formic acid and acetonitrile under gradient elution. Column comparison, aqueous-fraction experiments, and SRM 870 probe testing indicated a mixed retention mechanism involving hydrophilic partitioning into a water-enriched interfacial layer and reversible interactions with accessible residual silanol sites. Because the method prioritizes MS compatibility and reproducible multi-residue quantification, compound-specific MRM transitions and matrix-matched calibration were used to ensure selectivity and quantitative reliability. The method showed good linearity (R² > 0.99), satisfactory accuracy (71.2–99.8%) with reported standard deviations, acceptable precision (CV ≤ 11.3%), and low detection limits (LODs 3–15 μg/kg; LOQs 10–50 μg/kg). Overall, this work demonstrates that a conventional C 18 column can exhibit pronounced hydrophilic selectivity under high-organic conditions, offering a practical, MS-friendly alternative for aminoglycoside residue analysis and informing future stationary-phase development for hydrophilic separations.
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Analytical Methods
·
2023
·Vol. 15
·期 7
·884-895
·
IF 3.1
Xin Wang,Xiaoqi Sun,Yueying Jin,Shengyu Cheng,Yu Han,Minghui Zhang,Lingli Zhang,Xi-Ling Li,Chun-Yan Xu,Jun Zhe Min
This study reports a novel fluorescent chiral derivatization reagent, 4-(N,N-dmethylaminosulfonyl)-2,1,3-benzoxadiazole-(2-succinimidoxy)-trans-2-methyl-L-proline (DBD-S-M-Pro), with a benzoxadiazole structure containing an N-hydroxysuccinimide activation group. DBD-S-M-Pro targets chiral amino-functional compounds under alkaline conditions without a condensation agent. Gradient elution was performed on a BEH C18 (100 × 2.1 mm, 1.7 μm) column with a mobile phase of 0.05% formic acid (FA) in 10 mM ammonium acetate (CH3COONH4) and 0.1% FA in acetonitrile or methanol. The efficiency of the chiral resolution was evaluated under excitation and emission wavelengths of 450 nm and 560 nm, respectively. The 19 chiral amino acids were separated in the range of 1.45–14.84. The resolutions of almost all DL-amino acids exceeded 1.5; the exceptions were serine (Ser) and lysine (Lys), with resolutions of 1.45 and 1.46, respectively. In addition, a new approach was devised for the simultaneous analysis of four chiral amino acids (DL-Glu, DL-Ala, DL-Val, and DL-Phe) in human hair. These amino acids were analyzed in the range of 12.5–400 pmol, with R2 ≥ 0.9990, limits of detection (S/N = 3) of 4–10 pmol, and intraday and interday precisions of 0.57–6.23%. The average spikes in the hair recoveries were 89.76–111.54%, and the matrix effects were 92.47–102.40%. Next, the contents of free chiral amino acids in the hair samples of 10 healthy volunteers (five males and five females) were analyzed with this method, and the differences were compared. The developed DBD-S-M-Pro provides a novel strategy for the sensitive determination of free chiral amino acids in living organisms.
研究领域:
分析化学
·
生物化学
·
药学
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CATALYSIS TODAY
·
2018
·
IF 4.667
Guanhua Xing,Siyang Liu,Qingxin Guan,Wei Li
This paper focused on the isomerization and cracking of n -alkanes to synthesize bio-jet fuel. A series of SAPO-11 supports with different Si/Al molar ratio (0.2, 0.4, 0.6) were prepared, characterized, and evaluated by the hydroisomerization on hydrodeoxygenation product of castor oil. As a result, a Ni-Mo/SAPO-11(0.4) catalyst with hollow tubular structure and moderate acidic strength exhibited optimum conversion (97.2%) and jet fuel selectivity (81.6%) in the reaction. Subsequently, Ni-Mo/SAPO-11(0.4) was selected to carry out further experiments on studying the hydroisomerization and hydrocracking of long chain n -alkanes. The results show that increasing temperature may promote hydroisomerization and hydrocracking. Temperature is more favorable for the reaction with the length of the carbon chain decreasing, no matter conversion, hydrocracking degree or isomerization tendency. The product selectivity of different n -alkanes is associated with the carbon number. In reaction to n -C 18 , Ni-Mo/SAPO(0.4) surpassed the other two catalysts in the conversion and i/n ratio at 623 K which is the optimum temperature.
研究领域:
催化化学
·
能源科学
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高影响因子
Food Chemistry: Molecular Sciences
·
2026
·
IF 4.8
Yongbang Si,Yongna Song,Dong Huang,Yifei Chen,Jun Sun,Zezhong Wu,Rui Ma,Guoliang Sun,Yuqiong Meng
Driven by sustainability, plant oils are increasingly used to replace fish oil in aquafeeds, thereby altering muscle fatty acid composition and potentially affecting volatile profiles. Lipoxygenases (LOXs) participate in fatty-acid bioconversion, but the role of 5-lipoxygenase (5-LOX) in non-immune fish muscle remains unclear. We hypothesized that acute intragastric exposure to plant-derived C18 fatty acids would be associated with changes in muscle 5-LOX protein abundance and volatile profiles in triploid rainbow trout. Using an acute oral gavage model, trout received oleic, linoleic, or α-linolenic acid, alone or with the selective 5-LOX inhibitor zileuton. Muscle samples were analyzed for 5-LOX mRNA expression, detectable protein abundance, and volatile profiles. Detectable 5-LOX protein abundance was low under basal conditions and changed after fatty-acid administration, with linoleic acid showing the clearest increase. Several major volatiles also changed across treatments. Under acute fatty-acid exposure, zileuton reduced hexanal concentration by 85.7% (221.2 to 31.6 ng/g) and decreased the nonanal odor activity value by 34.4%. These results indicate that acute plant-derived C18 fatty acid exposure was associated with changes in detectable muscle 5-LOX protein abundance and volatile profiles in triploid rainbow trout. The inhibitor-associated volatile changes further support the possibility that multiple oxidative processes may be involved under these acute conditions.
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ANALYTICAL LETTERS
·
2021
·
IF 2.329
Mengke Jin,Jiajia Shan,Xue Wang,Tao Ren,Xinjing Li
Florfenicol has been widely used in livestock and aquaculture to treat bacterial infections. Due to the abuse of antibiotics, florfenicol has been released into the environment. Thus, there is a need for the rapid determination of trace levels of florfenicol in the environment. To improve the sensitivity for florfenicol, solid phase extraction (SPE) was employed to enrich florfenicol from multi-residue antibiotic mixtures followed by in situ determination by surface-enhanced Raman spectroscopy (SERS). Activated carbon, C18, graphene oxide (GO), and sand were applied to concentrate florfenicol in water. Silver nanoparticles (AgNP)-coated silicon (AgNPs@Si) substrate was employed to determine florfenicol enriched on the adsorbent. The results showed that the detection limit of florfenicol in water was 2.8 × 10−9 mol/L following the adsorption and enrichment of activated carbon. Besides, activated carbon was performed to extract florfenicol from the multi-residue antibiotic mixtures, including enrofloxacin, norfloxacin, and florfenicol. The results showed that activated carbon combined with SERS specifically determined florfenicol in complex antibiotic mixtures with a detection limit of 2.8 × 10−9 mol/L. Moreover, to better understand the adsorption mechanisms, the mechanism of the interaction of florfenicol with activated carbon was investigated.
研究领域:
环境科学
·
分析化学
·
药物分析
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高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zihan Xu,Shanshan Liu,Mingyue Shen,Jianhua Xie,Jun Yang
Trans fatty acids (TFAs), associated with the risks of coronary heart disease and diabetes, are formed by isomerization of unsaturated fatty acids during refining of linseed oils. In this study, TFAs and the chemical characteristics (acid value, peroxide value, carbonyl compounds, bioactive minor components and fatty acids) in 32 commercial linseed oils were investigated, and the correlation among them were further analyzed. Results showed that C18:3 TFAs were predominant TFAs in linseed oils and about 9% of the samples had TFA contents above 2 g/100 g fat, as well as the average level of TFA in the refined samples was higher than that in the unrefined oils. The correlation analyses suggested C18:3 TFAs exhibited significant negative correlations with acid value, levels of acetone, trans -2-nonenal, campesterol and α-linolenic acid. These results provided a comprehensive insight of TFAs in linseed oil and had important implications for consumers and linseed oil industry.
研究领域:
食品科学
·
营养学
·
化学
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Food Analytical Methods
·
2026
·Vol. 19
·期 1
·60
·
IF 3
Hussain Aisha,Wu Jiapeng,Wen Jiaxi,Xiong Chaomei
Sudan dyes, a class of azo dyes, are genotoxic and carcinogenic to humans. However, they are still used illegally to color food products and spices due to their color fastness. In this study, multi-functionalized magnetic silica nanoparticles (C 18 /DVB/NVP(4:1)-Fe 3 O 4 @SiO 2 MNPs) were synthesized, and solid–liquid extraction (SLE) coupled with magnetic solid phase extraction (MSPE) was employed for the extraction and preconcentration of five Sudan dyes (Sudan I − IV, Sudan Red 7B), followed by high-performance liquid chromatography/ultraviolet spectroscopy (HPLC–UV) for their detection in powdered chili pepper samples. The SLE conditions for the analytes in powdered chili pepper samples were optimized. Under optimal conditions, the extraction recovery ranged between 78.0% and 106.9%. We investigated and optimized the potential factors that may affect the efficiency of MSPE. Under these optimal conditions, calibration curves showed good linearity ( R 2 > 0.9989) across the tested concentration range of 0.98 to 125 µg/g. The limits of detection for the five Sudan dyes were in the range of 0.06–0.26 µg/g. The intra-day and inter-day accuracy ranged from 93.5% to 106.3% with a relative standard deviation (RSD) of not more than 9.1%. The developed method was successfully applied to analyze ten different commercial brands of powdered chili pepper samples purchased from markets in Wuhan, confirming its feasibility for routine analysis of illegal Sudan dyes in these samples.
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Nature Communications
·
2021
·Vol. 12
·期 1
·1-10
·
IF 14.919
Zhang Lufeng,Zhang Liang,Deng Hui,Li Huan,Tang Wentao,Guan Luyao,Qiu Ye,Donovan Michael J.,Chen Zhuo,Tan Weihong
Helicobacter pylori infection is a major etiological factor in gastric diseases. However, clinical antibiotic therapy for H. pylori is limited by continuously decreased therapeutic efficacy and side effects to symbiotic bacteria. Herein, we develop an in vivo activatable pH-responsive graphitic nanozyme, PtCo@Graphene (PtCo@G), for selective treatment of H. pylori . Such nanozymes can resist gastric acid corrosion, exhibit oxidase-like activity to stably generate reactive oxygen species only in acidic gastric milieu and demonstrate superior selective bactericidal property. C 18 -PEG n -Benzeneboronic acid molecules are modified on PtCo@G, improving its targeting capability. Under acidic gastric pH, graphitic nanozymes show notable bactericidal activity toward H. pylori , while no bacterial killing is observed under intestinal conditions. In mouse model, high antibacterial capability toward H. pylori and negligible side effects toward normal tissues and symbiotic bacteria are achieved. Graphitic nanozyme displays the desired enzyme-like activities at corresponding physiological sites and may address critical issues in clinical treatment of H. pylori infections.
研究领域:
纳米技术
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生物医学工程
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微生物学
·
药学
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