RSC Advances
·
2016
·Vol. 6
·期 49
·42994-42999
·
IF 3.289
Zonggang Mu,Lei Jiao,Qin Wei,He Li
Nobel metal nanoparticles have attracted intense attentions in biological immunoassay due to the inhereted good catalytic activity. In this study, a new kind of nanocomposite of ternary Pt@Pd@Ru nanodendrite-decorated graphene oxide (GO–Pt@Pd@Ru) was prepared as a signal label for ultrasensitive detection of carcinoembryonic antigen (CEA). With a sandwich type immunoassay, the established electrochemical immunosensor showed highly sensitive detection performance towards CEA in a linear range (0.1 pg mL−1 to 9 ng mL−1) with an ultralow detection limit of 0.05 pg mL−1. The enhancing detection performance could be attributed to: ternary Pt@Pd@Ru nanodendrites have exhibited superior electrochemical reduction activity towards H2O2, then GO–Pt@Pd@Ru can combine all generated electrochemical reduction current towards H2O2 by the decorated Pt@Pd@Ru nanodendrites, which can significantly amplify the detection signal.
研究领域:
生物医学工程
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分析化学
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纳米材料科学
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DOI
高影响因子
CANCER SCIENCE
·
2018
·Vol. 109
·期 4
·1185-1194
·
IF 4.372
Hai-Ning Liu,Tao-Tao Liu,Hao Wu,Yan-Jie Chen,Yu-Jen Tseng,Can Yao,Shu-Qiang Weng,Ling Dong,Xi-Zhong Shen
Recently, many new diagnostic biomarkers have been developed for colorectal cancer. We chose 2 methods with high diagnostic efficiency, the detection of serum microRNA and metabolomics based on gas chromatography/mass spectrometry (GC/MS), and aimed to establish appropriate models. We reviewed the diagnostic value of all microRNA identified by previous diagnostic tests. We selected appropriate microRNA to validate their diagnostic efficiency, and determined the optimal combination. We included 85 patients with colorectal cancer (CRC) and 78 healthy controls (HC) and detected the expression of the microRNA. GC/MS analysis was conducted, and we used 3 multivariate statistical methods to establish diagnostic models. The concentrations of carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) were detected for comparison with the novel models. Ultimately, 62 published studies and 63 microRNA were included in this review. MiR-21, miR-29a, miR-92a, miR-125b and miR-223 were selected to further validate their diagnostic value. The serum levels of the 5 microRNA in CRC patients were significantly higher than those in the HC. The combination of miR-21, miR-29a, miR-92a and miR-125b had the highest area under the curve (AUC) at 0.952, with a sensitivity of 84.7% and a specificity of 98.7%. The GC/MS analysis exhibited an excellent diagnostic value and the AUC reached 1.0. With regard to traditional biomarkers, the AUC of CEA and CA19-9 were 0.808 and 0.705, respectively. The application prospects are good for microRNA and metabolomics as new diagnostic methods because of their high diagnostic value compared with traditional biomarkers.
研究领域:
医学
·
分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Xiangfei Zheng,Guichun Mo,Yongyu He,Dongmiao Qin,Xiaohua Jiang,Weiming Mo,Biyang Deng
[email protected] QDs were synthesized by a simple one-step method. The prepared [email protected] quantum dots ( [email protected] QDs) composite showed a strong cathode ECL emission using potassium persulfate (K 2 S 2 O 8 ) as a co-reactant. The poly-(diallyldimethylammonium chloride) (PDDA) reduced graphene oxide (PDDA-rGO) composite provided a larger activity site could conjugate more [email protected] QDs and second antibody (Ab 2 ) to improve the sensitivity of the ECL immunosensor. The electrode modified with Au NPs was employed for immobilization of primary antibodies (Ab 1 ) and enhancing the electronic transmission rate of the immunosensor. A novel ECL immunosensor was constructed using Au NPs as a sensing platform and PDDA-rGO/ [email protected] QDs composite as signal probe for highly-sensitive carcinoembryonic antigen (CEA) detection in human serum. Under optimal conditions, a range of 0.0001–100 ng/mL and the detection limit of 0.029 pg/mL for CEA were obtained. This ECL immunosensor possesses long-term stability, good reproducibility and high sensitivity. These are promising qualities for detecting biomarkers, drugs, and environmental pollutants .
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
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DOI
高影响因子
NEUROBIOLOGY OF DISEASE
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2022
·
IF 7.046
Shilang He,Xuelin Huang,Jun Zheng,Yuehong Zhang,Xiangcai Ruan
Depressive symptoms comorbid with chronic pain are a common health problem, but the underlying neural circuit mechanisms remain elusive. Here, we identify a glutamatergic projection from the nucleus of the solitary tract (NTS) to the central nucleus of the amygdala (CeA) that mediates depression-like behaviors in a chemotherapy-induced neuropathic pain model. Inhibition or ablation of the glutamatergic NTS neurons alleviates depressive but not hypersensitive behaviors in these mice. The projected neurons form excitatory synapses with somatostatin-expressing neurons in the CeA. Silencing the NTS-CeA projection alleviates depressive but not hypersensitive behaviors, whereas activating the proection promotes depressive behaviors. In addition, in naïve mice, activation of the NTS-CeA projection induces obvious depressive behaviors that can be blocked by silencing the CeA somatostatin-expressing neurons. Together, we reveal a modulatory role of the NTS and its glutamatergic projection to the CeA circuit in modulating depression-like behaviors comorbid to chronic pain.
研究领域:
神经科学
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疼痛研究
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行为神经科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yu Li,Ying Chen,Dongmei Deng,Liqiang Luo,Haibo He,Zhenxin Wang
In this work, a water-dispersible graphene/amphiphilic pyrene derivative nanocomposite has been synthesized for electrochemical immunosensing carcinoembryonic antigen (CEA). Water-soluble 4-armed poly(ethylene glycol)-NH 2 is immobilized on graphene through noncovalent functionalization with the assistance of pyrene as an anchor group. The noncovalent functionalization strategy can significantly improve the hydrophilicity of graphene and loading capacity of gold nanoparticles (AuNPs) on graphene, resulting in efficient immobilization of antibodies on the nanocomposite. The morphology and conductivity of the as-prepared nanocomposites were characterized by scanning electron microscopy, Fourier transform infrared and UV–vis spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). CEA monoclonal antibody (anti-CEA) is immobilized on AuNPs via gold-thiol chemistry to construct the electrochemical immunosensing platform, and the surface binding events between anti-CEA and CEA is achieved by monitoring the change of the electrochemical impedance response. Under optimum conditions, the modified electrode exhibits a linear impedance response to CEA in a wide range of 0.1–1000 ng mL −1 ( R 2 = 0.9959) with low detection limit of 0.06 ng mL −1 ( S/N = 3).
研究领域:
生物医学工程
·
分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Hongye Yan,Lei Jiao,Hengjia Wang,Weiqing Xu,Yu Wu,Wenling Gu,Dan Du,Yuehe Lin,Chengzhou Zhu
Enzyme-linked immunosorbent assay (ELISA) has been widely used in the area of biological analysis, while there are still some challenges getting in the way to ensure both excellent sensitivity and reliable accuracy. To overcome these obstacles, we simulated the “smart” sense-and-treat carriers in a drug delivery system to fabricate an improved ELISA, employing zeolitic imidazolate framework-8 (ZIF-8) as the carrier to deliver the tracer agent carbon dots (CDs) and the “drug” thymolphthalein (TP), which was referred to as a “sense-and-treat” ELISA. In the “sense” part, the strong fluorescence intensity of CDs could be observed directly to achieve the sensitive detection of the target. In the “treat” part, after stimulation of alkaline solution, TP was released from ZIF-8 carriers and generated a color change with an obvious absorption, which was beneficial for increasing the sensitivity of this ELISA due to the high loading of the TP. Finally, we took carcinoembryonic antigen (CEA) as a model and performed dual-modal detection using this improved “sense-and-treat” ELISA to accomplish quantitative determinations sensitively and accurately. In addition, the results indicated by the two parts kept good concordance, which could demonstrate the reliable accuracy of this ELISA.
研究领域:
生物分析
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医学诊断
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DOI
高影响因子
Micro & Nano Letters
·
2021
·Vol. 16
·期 4
·257-262
·
IF 1.102
Xiao Wang,Mei Wu,Chulei Zhao,Xiaochen Liao,Mengmeng Zhang,Lisha Mei,XiuWen Qiao,Chenglin Hong
Using Ag-CoFe 2 O 4 -GO nanocomposite as biosensor interface, a novel unlabelled electrochemical immunosensor was designed to detect tumour marker carcinoembryonic antigen. Here, Ag-CoFe2O4-GO was synthesized by one-pot method as an immunological probe. They have the following advantages: (1) Silver nanoparticles have a strong redox capacity under applied voltage and good biocompatibility, so they can bind more antibodies; (2) GO has a large specific surface area, which can load more Ag NPs and CoFe 2 O 4 nanoparticles, and has excellent conductivity; (3) CoFe 2 O 4 is widely favoured by researchers due to its advantages such as low production cost and simple preparation process, The prepared Ag-CoFe 2 O 4 -GO nanocomposite can amplify the electrochemical signal; (4) The presence of GO can effectively prevent the agglomeration of Ag NPs nanoparticles and CoFe 2 O 4 nanoparticles, promote the formation of electron transfer network, and contribute to the electron transfer of sensors. Under optimal condition, the current signal and the concentration of carcinoembryonic antigen were linear in the range of 1 pg mL < sp > −1 < /sp > ∼ 80 ng mL < sp > −1 < /sp , > and the detection limit was 0.3 pg mL < sp > −1 < /sp > (S/N = 3). Under certain conditions, the manufactured immunosensor has good reproducibility, selectivity and excellent stability. Thus it has certain clinical value for the detection of carcinoembryonic antigen.
研究领域:
生物医学工程
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分析化学
·
纳米技术
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DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Xia Wang,Zhe Qin,Fei Zhang,Chong Li,Xianxian Yuan,Jing Yang,Huaixia Yang
Carcinoembryonic Antigen (CEA), an acidic glycoprotein with human embryonic antigen properties, is found on the surface of cancer cells that have differentiated from endodermal cells. This paper presents a label-free electrochemical immunoassay for the dual amplification detection of CEA using gold nanoparticles loaded with polypyrrole polydopamine (Au/PPy-PDA) and polymerized polycaprolactone (Ng-PCL) prepared by ring-opening polymerization (ROP). First, the composite Au/PPy-PDA was adhered to the electrode surface. Then, gold nanoparticles form a Au–S bond with the sulfhydryl group in Apt1 to secure it on the electrode surface. Subsequently, the non-specific binding sites on the electrodes surface are closed by bovine serum albumin (BSA). Next, CEA is dropped onto the electrode surface, which is immobilized by antigen-antibody specific recognition, and the carboxyl-functionalized Apt2 forms a "sandwich structure" of antibody-antigen-antibody by specific recognition. Polymeric Ng-PCL is adhered to the electrode surface, leading to an increase in the electrochemical impedance signal, resulting in a complete chain of signal analysis. Finally, the response signal is detected by electrochemical impedance spectroscopy (EIS). Under optimal experimental conditions, the method has the advantages of high sensitivity and wide linear range (1 pg mL −1 ∼100 ng mL −1 ), and the lower limit of detection (LOD) is 0.234 pg mL −1 . And it has the same high sensitivity, selectivity and interference resistance for the real samples detection. Thus, it provides a new way of thinking about biomedical and clinical diagnosis.
研究领域:
生物医学
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临床诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2026
·
IF 1.8
Xuejun Liang,Jiuxin Cui,Kehong Liu,Jichao He,Qincong Feng,Yabo Wang
Early detection of cancer biomarkers is essential for accurate diagnosis and timely therapeutic intervention. Carcinoembryonic antigen (CEA), a nonspecific and broad-spectrum tumor-associated biomarker, is extensively employed for the early screening and diagnosis of multiple malignancies. In recent years, electrochemical nanopipette sensing technology has been increasingly explored for clinical disease detection owing to its high sensitivity, low background interference, and inherent suitability for single-cell analysis. Nucleic acid aptamers, characterized by target-specific three-dimensional conformations, are capable of binding a wide range of molecular targets with high affinity and selectivity. In this work, a functional nanopipette microelectrode was rationally designed and fabricated by immobilizing nucleic acid aptamers onto electrochemical nanopipette electrodes, enabling label-free detection of CEA at the single-cell level. Under optimized conditions, the resulting sensor achieved a limit of detection as low as 1.27 pg/mL.
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DOI
高影响因子
ACS Omega
·
2025
·
IF 4.3
Zhengyao Zhang,Zhi Li,Yuhang Jin,Xu Gao,Zekai Zhu,Hangyu Zhang,Bin Wu,Bo Liu
Colloidal gold immunochromatography has emerged as a pivotal platform for point-of-care diagnostics, yet challenges persist in stabilizing nanoparticle-antibody interactions and ensuring batch-to-batch reproducibility. To address these limitations, we engineered a nanogold-affinity peptide probe for the rapid detection of the lung cancer biomarker carcinoembryonic antigen (CEA), leveraging phage-display-derived peptides as biorecognition elements. The probe was synthesized by conjugating CEA-specific affinity peptides to polyethylene glycol (PEG)-functionalized gold nanoparticles (AuNPs) via covalent amide bonding, ensuring precise orientation and enhanced colloidal stability. Systematic optimization of reaction parameters, including the PEGylation time, peptide-to-nanoparticle ratios, and centrifugation conditions, yielded a robust preparation protocol. The resulting immunochromatographic test strip demonstrated a detection limit of 2.5 ng/mL for CEA, surpassing the clinical threshold of 5 ng/mL, and exhibited 91.7% accuracy in clinical serum samples. Notably, the substitution of antibodies with synthetic affinity peptides reduced costs by approximately 8-fold while maintaining high specificity and resistance to nonspecific binding. This work not only advances the integration of biomolecular engineering and nanotechnology for diagnostic applications but also establishes a scalable framework for developing stable, low-cost biosensors targeting macromolecular biomarkers.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2018
·Vol. 18
·期 12
·8085-8093
·
IF 1.354
Qi Jianrong,Hu Meixin,Li Hongxia,Jing Ruan,Shen Guangxia
Due to the dual role as an electron acceptor and an electron donor in solution, carbon dots (Cdots) have broad applications in environmental analysis, biological detection, and biosensors. Herein, we report a facile-green strategy for a large-scale synthesis of fluorescent N, P-doped
carbon dots (N, P-Cdots) with an absolute quantum yield of 66.08% by a simple one-step thermal decomposition. Glucose was selected as a carbon precursor and tryptophan (Trp) as an N-doping and passivation reagent. Organic polar solvents with a high boiling point, i.e., ethylene glycol and
glycerol, were used as the reaction medium, and phosphoric acid was employed as a P source and oxidation accelerator. It is shown that the emission wavelength of the N, P-Cdots can be tuned by adjusting the reaction conditions, such as mass ratio, heating time, temperature, and medium, without
further passivation. Finally, advantage was taken of the superior fluorescent characteristics of N, P-Cdots to detect selectively and with high sensitivity a cancer marker, carcinoembryonic antigen (CEA), based on the fluorescent quenching mechanism. Additionally, CEA was also detected in
human serum samples with high efficiency and RSD, further confirming that the proposed method has a good consistency and stability for supersensitive fluorimetric detection of cancer markers.
研究领域:
分析化学
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生物医学
·
材料科学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 8
·3940-3946
·
IF 3.591
Bo Liu,Luanying Yang,Gang Wang,Sha He,Xiaobo Wang,Ling Ye
In this study, a simple and low-cost electrochemical immunosensor for the detection of tumor markers (carcinoembryonic antigen, CEA, as a model) was investigated. The self-polymerization of dopamine was used to provide a secondary reaction platform, and a more stable dithiol compound (thioctic acid) as a spacer was fixed on the platform via the Michael's addition reaction. A dithiol-functionalized platform was used for the covalent immobilization of antibodies, and the structure showed significant changes in the atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) images. The electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) measurements were performed to test the performance of a double-antibody sandwich structure. The designed CEA immunosensor exhibited a linear response over a concentration range of 0.05–80 ng mL−1 with a low detection limit of 1.21 pg mL−1, and the results demonstrated a potential application for clinical diagnosis.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
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