CATALYSIS TODAY
·
2020
·
IF 5.825
Jinfeng Han,Caixia Liu,Qingling Liu,Shuangchun Lu,Yalian Bi,Xiaohan Wang,Mingyu Guo,Chunfeng Song,Na Ji,Xuebin Lu,Degang Ma,Zhenguo Li
Novel Al-rich OFF-CHA twin-crystal zeolite was synthesized by one-pot hydrothermal method, and applied in ammonia-selective catalytic reduction (NH 3 -SCR) after copper ion exchange. Results indicated that Cu content in Cu-OFF-CHA played a significant role in NH 3 -SCR reaction. The insufficiency of copper activity sites and the formation of CuOx were the main reasons affecting low and high temperature SCR activity of catalyst, respectively. Cu 3.1 -OFF-CHA, with the optimal copper content, demonstrated over 90 % NOx conversion and exhibited excellent N 2 selectivity in the range from 160 to 550 ℃. Further research verified that the SCR activity of Cu 3.1 -OFF-CHA maintained above 80 % in the temperature range from 180–450 °C after hydrothermal treatment 16 h. The reason might be that Cu 2+ in Cu-OFF-CHA was migrated from CHA to OFF sites after hydrothermal treatment, and Cu 2+ in OFF sites on Cu-OFF-CHA was more stable, resulting in less conversion of the active site to CuOx clusters. Cu-OFF-CHA has the potential to be applied in SCR after-treatment systems of diesel exhaust due to the excellent SCR activity and hydrothermal stability.
研究领域:
催化化学
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环境化学
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材料科学
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化学工程
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Chunyu Su,Qianying Wang,Bingying Jiang,Ruo Yuan,Yun Xiang
A highly sensitive and aptamer-based fluorescent assay for flunixin is established. • DNAzyme walker/self-replication CHA circuit enables significant signal amplification. • Selective detection of fluxnixin in complex milk sample matrix by this assay is verified. Flunixin is a widely used veterinary non-steroidal anti-inflammatory drug, and its improper administration can lead to residue contamination in animal-derived foods, which poses a pressing need for rapid and sensitive residue monitoring. Herein, we develop an aptamer-based fluorescent assay that integrates a Mg 2+ -dependent DNAzyme walker with an autocatalytic catalytic hairpin assembly (CHA) circuit to achieve cascade signal amplification for flunixin determination. In the presence of flunixin, target binding to an aptamer/DNAzyme duplex activates the DNAzyme, which repeatedly walks on gold nanoparticle (AuNP) tracks and cleaves rA-containing substrate hairpins, continuously releasing single-stranded trigger sequences. These triggers initiate a three-hairpin CHA process to generate Y-shaped DNA products and simultaneously open FAM/Dabcyl-quenched reporter hairpins, restoring fluorescence. Notably, the Y-shaped products regenerate trigger-equivalent sequences, driving a self-replication CHA cycle that enables sustained reporter opening and markedly enhanced fluorescence output. Under optimized conditions, the proposed strategy achieves a detection limit of 0.41 nM with good selectivity against potential interferents. The assay was further validated by determining flunixin in milk samples, supporting its applicability for sensitive analysis of small-molecule veterinary molecule residues. Download: Download high-res image (44KB) Download: Download full-size image
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
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IF 8.4
Xinghui Zhou,Chengpeng Li,Wanxue Wang,Feng Zhang,Yi Zhou,Danping Chen,Zhenchao Wang
N 6 -methyladenosine (m 6 A) is a crucial post-transcriptional epigenetic modification in RNAs which associates with numerous cancers development. Here, a cost-effective, and easy-to-operate fluorescence strategy for m 6 A analysis was developed based on the different cleavage efficiency of 8–17 DNAzyme for methylation and unmethylation sites. By arranging 8–17 DNAzyme to recognize and cleave 5'-rAG-3' and 5'-rm 6 AG-3' motifs in the target sequence respectively, various short ssDNA oligoes were produced, leaving different amounts of target sequence. Benefiting from the varying degrees of retardation of CHA amplification reaction, the tiny discrepancy between m 6 A and unmethylated adenine (A) can be distinguished. Moreover, we found that 8–17 DNAzyme was more sensitive to m 6 A than to A. Importantly, the strategy we proposed not only avoids sophisticated chemical labelling and expensive proteins, but also can be easily performed on the ordinary heating apparatus of accessible laboratory. The DCHA strategy demonstrated good analytical performance in complex biological matrices, and was successfully applied to hepatocellular carcinoma-related m 6 A analysis in CTNNB1, MGAT5 and SOCS-2 mRNAs with satisfactory results, showing its potential in clinical analysis.
研究领域:
分子生物学
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生物化学
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癌症生物学
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表观遗传学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CATALYSIS
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2016
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IF 2.628
Jixing Liu,Jian Liu,Zhen Zhao,Weiyu Song,Yuechang Wei,Aijun Duan,Guiyuan Jiang
A series of meso-microporous copper-supporting chabazite molecular sieve (Cu-SAPO-34) catalysts with excellent performance in low-temperature ammonia selective catalytic reduction (NH 3 -SCR) have been synthesized via a one-pot hydrothermal crystallization method. The physicochemical properties of the catalysts were characterized by scanning electron microscopy, transmission electron microscopy , N 2 adsorption-desorption measurements, X-ray diffraction, 27 Al magic angle spinning nuclear magnetic resonance , diffuse reflectance ultraviolet-visible spectroscopy, inductively coupled plasma-atomic emission spectroscopy, X-ray photoelectron spectroscopy , temperature-programmed reduction measurements, and electron paramagnetic resonance analysis. The formation of micro-mesopores in the Cu-SAPO-34 catalysts decreases diffusion resistance and greatly improves the accessibility of reactants to catalytic active sites. The main active sites for NH 3 -SCR reaction are the isolated Cu 2+ species displaced into the ellipsoidal cavity of the Cu-SAPO-34 catalysts.
研究领域:
材料化学
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环境催化
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多孔材料
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工业催化
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能源与环境科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2025
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IF 10.5
Jing Peng,Simin Liu,Ziyue Qiu,Luyin Zhang,Ziyan Dong,Qianying Zhou,Ting Huang,Yanfei Zhang,Jingyu Shi,Jin-Xiang Chen,Zong Dai,Jun Chen
miRNA is an effective disease diagnostic biomarker. Developing efficient miRNA in living cells analysis methods, holds significant importance for disease diagnosis and understanding disease progression. Strategies combining DNA nanostructures and enzyme-free amplification have been developed for highly sensitive and rapid imaging of miRNA. However, due to nonspecific amplification of enzyme-free amplification inherent in the probe assembly process and during transfection, these strategies suffer from significant background signals and reduced sensitivity, limiting their further application. To overcome these limitations, we have developed a DNA tetrahedral-based and photocontrolled intramolecular CHA strategy (TCHA-P) to achieve spatiotemporal, highly sensitive, and accurate imaging of miRNA in living cells. In this strategy, the DNA tetrahedron not only serves as a transfection vector but also enhances the local concentration of reactant probes, boosting the reaction kinetics and sensitivity of CHA. Additionally, the light-controlled group (PC-linker) can trigger CHA at selected times and locations, enhancing the spatiotemporal precision of miRNA imaging and ensuring high sensitivity and accuracy by eliminating nonspecific amplification during the assembly and transfection processes. Using miR-10b as a proof-of-concept model, the proposed strategy demonstrated higher signal-to-noise ratio compared to traditional CHA, with a detection limit of 28 pM. Living cell imaging experiments showed that the strategy could effectively differentiate the expression of miR-10b between normal breast cells and breast cancer cells, providing high imaging contrast. The strategy holds promise for broadening the path of nucleic acid amplification-based imaging and is expected to become an effective tool in nucleic acid-based disease diagnosis, treatment, and drug development.
研究领域:
分子生物学
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纳米生物学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Shulian Bai,Ting Wang,Zhang Zhang,Shangchun Sheng,Wen Yu,Guoming Xie
Adopting the concept and operation of hybridization-based associative toehold activation (HATA) concatemer induced catalyzed hairpin assembly (CHA), a novel colorimetric biosensor was developed for detecting target DNA and human alpha-thrombin. The assemble of separate DNA hairpin components can be triggered by specific target binding that are otherwise unable to spontaneously happen. In the presence of four ingeniously designed hairpin structures, the target molecule can trigger two cascade recycling reactions: HATA concatemer reaction and CHA reaction. In the HATA concatemer reaction, the toehold and branch migration domain overhang at the 5′ end and 3′ end of hairpin probe H1, respectively. Upon the addition of target molecule, plenty of toeholds and branch migration domains can be brought into close proximity via DNA self-assemble. Then, the toeholds and branch migration domains of HATA products serve as the trigger of CHA. In this concatemer recycling, numerous guanine rich DNA duplexes can be produced through the highly effective use of target molecule. The proposed sensing system exhibits a remarkable analytical performance and an ultrahigh discrimination capability for proximal mismatch. Under optimal conditions, the calibration curves have a good linearity from 1 pM to 75 nM and 1 pM to 2.5 nM for target DNA and human alpha-thrombin, respectively. The corresponding detection limits are 0.14 pMand 0.68 pM Therefore, this new biosensor has a great potential for further applications in biomedical research and demonstrates excellent versatility for different analytes.
研究领域:
生物技术
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生物化学
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分子生物学
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分析化学
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DOI
高影响因子
PETROLEUM CHEMISTRY
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2022
·Vol. 62
·期 8
·962-971
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IF 1.258
Hao Zongyi,Niu Li,Long Xuefeng,Li Hongwei,Wang Dongliang,Ji Dong,Zhao Xinhong
Abstract Two different seed crystals (K–CHA and nano-scaled SAPO-34) were used to promote the rapid interzeolite conversion of USY and fumed silica to pure SSZ-13 zeolite phase via a grinding route. The results show that both seeds can shorten the crystallization time of SSZ-13 zeolite to 24h, which is much shorter than the syntheses in the absence of seeds. The phase purity, morphology, textural parameters, elemental compositions and acid properties of two typical H–SSZ-13 samples with seeds were analyzed by XRD, SEM, N 2 physisorption, EDS and NH 3 –TPD techniques in detail, and compared with the sample without seeds and a reference sample. The results indicate that the introduction of seeds can effectively modify the acid properties of H–SSZ-13, especially their medium strong acid sites. The methanol-to-olefins (MTO) reaction was employed as a probe to evaluate the catalytic stability of above four H–SSZ-13 catalysts. The results reveal that the catalytic lifetime of the two catalysts using seeds is much longer than that of the one without seeds and the reference sample H–SSZ-13–RS. It was speculated that the appropriate concentration of medium strong acid sites may play a pivotal role in prolonging their catalytic lifetime. Here our findings may throw light on the rational design of long-lifetime H-SSZ-13 catalysts in the MTO reaction.
研究领域:
化学工程
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催化
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材料科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2026
·
IF 11.8
Yan Wang,Boya Xu,Zehan Zeng,Haixin Wang,Lin Shi,Wanting Liu,Yanyan Chen,Xuexian Deng,Jun Chen,Jin-Xiang Chen
Extracellular vesicle (EV)-based liquid biopsy holds great promise for glioma diagnosis, but its clinical translation remains hindered by inefficient isolation of disease-relevant EV subpopulations and insufficiently integrated signal validation. Herein, we develop a platform that integrates a bifunctional nanozyme system (phosphatase-like MOF and peroxidase-like Pt) with a CHA-CRISPR/Cas12a cascade, achieving selective isolation and ultrasensitive detection of EGFR-positive glioma-derived EVs. Defective UiO-66-NH 2 loaded with Pt nanoparticles and functionalized with EGFR aptamers (UiO@Pt@Apt) is immobilized on Hook strand-modified glass 96-well plates via Apt-Hook hybridization. Upon introduction of EGFR-positive EVs, they specifically bind to aptamers on UiO@Pt@Apt and induce the release of UiO@Pt@Apt-EV complexes through perturbation and destabilization of the Apt-Hook interface. After EV lysis, EV-derived miRNA-21 activates the CHA-CRISPR/Cas12a cascade to generate a fluorescent signal, while the nucleotide fragments produced by Cas12a trans-cleavage are proposed to be hydrolyzed by phosphatase-like defective UiO-66-NH 2 to generate PO 4 3− . The PO 4 3− may contribute to Pt-mediated TMB oxidation, thereby supporting colorimetric signal amplification. The platform achieved a colorimetric EV detection limit of 427 particles/μL after coupling with the CHA-CRISPR system, representing a 6.9-fold improvement over the UiO@Pt@Apt system alone, and enabled a miRNA-21 detection limit of 87.0 fM. In plasma samples from 25 glioma patients and 20 healthy donors, the combined readout achieved an AUC of 0.968, showing numerically better discriminatory performance than either single readout, although the improvement was not statistically significant by DeLong analysis. This work provides a promising strategy for EV-based glioma liquid biopsy and offers a potentially adaptable framework for cascade-coupled dual-signal biosensing.
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Ye Tang,Dongyan Li,Ziyin Lu,Yaxi Wang,Wenjie Liu,Guoxing Jing,Wen Liu,Jianna Yu,Wenshan Li
The severe toxicity of Pb 2+ to human health, even at trace levels, the development of highly sensitive and dependable analytical methods remains of great importance. Here, we developed a label-free and enzyme-free colorimetric method for Pb 2+ assay by integrating a GR-5 DNAzyme recognition event with a catalytic hairpin assembly (CHA)-hybridization chain reaction (HCR) cascade amplification. Upon exposure to Pb 2+ , the GR-5 DNAzyme is specifically activated, catalyzing the site-specific cleavage of hairpin H1 to release the initiator strand T. The liberated T strand subsequently drives the CHA and HCR cascade reactions, generating long DNA superstructures enriched with multiple G-rich sequences. In the presence of hemin, these G-rich sequences fold into catalytically functional G-quadruplex/hemin DNAzymes, which enable a visible colorimetric signal generation. Under optimized conditions, the proposed colorimetric method delivered a linear response over the range of 50 pM to 50 nM and achieved a limit of detection of 16.71 pM. The practical applicability of the method was further demonstrated through Pb 2+ analysis in drinking water and fish samples, where satisfactory accuracy and reliability were obtained. In addition, inductively coupled plasma mass spectrometry was employed to validate the real-sample results, and the two methods showed good consistency. Owing to its simplicity, high sensitivity, and enzyme-free design, this colorimetric method shows strong potential for practical Pb 2+ monitoring in food safety and environmental analysis.
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
·
IF 3.154
Qian Luan,Ning Gan,Yuting Cao,Tianhua Li
A mimicking-enzyme-based colorimetric aptasensor was developed for the detection of kanamycin (KANA) in milk using magnetic loop-DNA-NMOF-Pt (m-L-DNA) probes and catalytic hairpin assembly (CHA)-assisted target recycling for signal amplification. The m-L-DNA probes were constructed via hybridization of hairpin DNA H1 (containing aptamer sequence) immobilized magnetic beads (m-H1) and signal DNA (sDNA, partial hybridization with H1) labeled nano Fe-MIL-88NH2-Pt (NMOF-Pt-sDNA). In the presence of KANA and complementary hairpin DNA H2, the m-L-DNA probes decomposed and formed an m-H1/KANA intermediate, which triggered the CHA reaction to form a stable duplex strand (m-H1-H2) while releasing KANA again for recycling. Consequently, numerous NMOF-Pt-sDNA as mimicking enzymes can synergistically catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) for color development. The aptasensor exhibited high selectivity and sensitivity for KANA in milk with a detection limit of 0.2 pg mL–1 within 30 min. The assay can be conveniently extended for on-site screening of other antibiotics in foods by simply changing the base sequence of the probes.
研究领域:
生物化学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yuanhuan Yang,Feng Gao,Ying Liang,Lichao Guo,Yang Pan,Peng Cao,Yue Zhang
The identification of Chinese medicinal herbs occupies a crucial part in the development of the food and drug market. Although molecular identification based on real-time PCR offers good versatility and uniform digital standards compared with traditional methods, such as morphology, the dependence on large-scale equipment hinders spot detection and marketable applications. In this study, we developed a DNA nanoclaw for colorimetric detection and visible on-site identification of Chinese medicines. When specific miRNA is present, the DNAzyme is activated and cleaves the substrate strand, triggering the catalytic hairpin assembly (CHA) reaction and forming branched DNA junctions on AuNP-I. This can then capture AuNP-II through hybridization and facilitate their aggregation, resulting in a noticeable color change that is observable to the naked eye. By harnessing the dual amplification of DNAzyme and CHA, this highly sensitive nanoprobe successfully achieved specific identification of Chinese medicines. This offers a new perspective for on-site testing in the herbal market.
研究领域:
生物技术
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纳米技术
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药学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Jing Bao,Changjun Hou,Yanan Zhao,Xintong Geng,Mickey Samalo,Huisi Yang,Minghong Bian,Danqun Huo
In present study, a sensitive and effective electrochemical microRNA (miRNA) sensing platform is successfully developed by integrating gold nanoparticles/polypyrrole-reduced graphene oxide (Au/PPy-rGO), catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) multiple signal amplification strategy. Firstly, Au/PPy–rGO was employed onto a bare GCE by electrodeposition that can greatly enhanced conductivity and effectively immobilize probes. Then, the thiolated capture probes (SH-CP) were self-assembled on the Au/PPy–rGO modified GCE via Au-S bond. The target miRNA triggered the dynamic assembly of the two hairpin substrates (H1 and H2), leading to the cyclicality of the target miRNA and the formation of H1–H2 complexes without the assistance of enzyme. Subsequently, the newly emerging DNA fragment of H2 triggered the HCR when a mixture solution (hairpins H3 and H4) and produced dsDNA polymers. Finally, a substantial amount of methylene blue (MB) as signal indicator was intercalated into the minor groove of the long dsDNA polymers to achieve detected electrochemical signal. The fabricated sensor is able to detect miRNA-16 (model target) with concentration range from 10 fM to 5 nM with a low detection limit (LOD) of 1.57 fM (S/N = 3). Current research suggests that the developed multiple signal amplification platform has a great potential for the applications in the field of biomedical research and clinical analysis.
研究领域:
生物医学工程
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分析化学
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纳米技术
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）